In order to investigate developmental habit and morphology of maize tillers, time and location of tiller development. number of tillers per plant, tiller angle, height and diameter of tillers and root systems of tillers were examined under field condition for maize with tillers. Materials used were mostly from Korean local lines and a few lines from other countries were also included for comparison. The time of the first tiller development was about 18 to 20 days after emergence when planted on May in Yusong. The second tiller appeared about 4 to 5 days after the first tiller appeared. The tiller number per plant varied with lines and hybrids and ranged from two to ten. The location of tiller development was usually basal nodes of the main stem. Each tiller appeared to have its own root system. The angle between tillers and main stem was variable depending upon the maizes and the tiller angle could be classified into three categories. The height of tillers was also variable and seemed to be under genetic control. The most productive tillers were found among the Korean local derivatives.
This experiment was undertaken in order to localize the labeled dbcAMP (dibutyryl cyclic AMP) in oocytes whose development has been suppressed by cold dbcAMP for 6 or 19 hours in vitro. Mouse oocytes were obtained from the ovaries of 3-4 week old A strain female mice, by puncturing the Graafian follicles in the modified Krebs-Ringer bicarbonate salt solution under the dissecting microscope. Those oocytes which have intact germinal vesicle were cultured in the basic culture medium supplemented with 0.4% bovine serum albumin (BSA). Cultivation of the oocytes was carried out in a microtube developed by Cho (1974). The cultures were then incubated in a humidified 5% $CO_2$ incubator maintained at $37^{\circ}C$ for 6 or 19 hours (Donahue, 1968). DbcAMP was added to culture medium for a final concentration of 100ug/ml, and $^3H-dbc$ AMP (specific activity 13 Ci/mM) for a final concentration of $40{\mu}Ci/ml$ was also added to the medium. For electron microscopic autoradiography, those oocytes recovered from the culture were washed with phosphate buffer (pH 7.4), and immediately prefixed in a 2.5% glutaraldehyde overnight and postfixed for 2 hours at $4 ^{\circ}C$ in 1% osmium tetroxide in phosphate buffer with pH 7.4 (Palade, 1952). After fixation, the materials were dehydrated in graded alcohol series and embedded in Epon 812 mixture based on the standard procedures (Luft, 1961). The thin sections $600-700{\AA}$ thick were mounted on the grids of 200 meshes. The grids containing sections were coated with a nuclear emulsion Kodak NTB-3 and stored in a cold dark box (at $4^{\circ}C$) for 3 weeks. After exposure, the samples were developed with Kodak D-19 and stained with uranyl acetate and lead citrate. Routine observation was made with Hitachi HU-11E electron microsocope. The results of the observation were as followings: 1. It was found that the labeled dbcAMP penetrated the egg plasma membrane and dispersed at random in the cytoplasm. 2. It was also observed that most of the labeled dbcAMP was attached to microfibrillar lattices portion of the oocyte cytoplasm. There fore, it is presumed that the receptor of the dbcAMP is localized in the microfibrillar lattices of the oocyte. 3. It also seems that some other cell organells such as mitochondria, Golgi complex, cortical granules are not directly related to the action of the dbcAMP. 4. The labeled dbcAMP was neither observed in the membrane nor in the nucleus. Therefore, it seems that there is no relationship between the concentration of dbcAMP and the nuclear membranous permeability. 5. There was no difference in number of dbcAMP particles when oocytes were cultured for 6 hours and 19 hours. 6. However, it was observed that, in same of the oocytes suppressed in germinal vesicle by dbcAMP for 19 hours, cell organells were moved and concentrated to a small portion of the cytoplasm, and that the morphology of the organells greatly changed to an abnormal. form. Therefore, it is supposed that those oocytes were in the process of degeneration. From the above results, it is expected that dbcAMP penetrated the egg membrane and was bound to the receptor which seems to be located in the microfibrillar lattiees portion, and that this dbcAMP-receptor complex inhibited some enzyme system of the oocytes which are essential for the germinal vesicle breakdown.
This study was performed to investigate the nutrition status by weight loss(diet) of 287 female college students, focus on questionnaire about diet survey. The subjects mainly had irregularly one or two meal per a day so food behavior was not good. Over half of subjects had a tendency to overestimate body shape of self. The desired weight(46.95$\pm$0.39kg) was significant lower than real weight(50.32$\pm$0.9)(p=0.0114). The time when diet is first tried was mainly after high school(94.51%). Mean weight loss was 1~4kg but most of subjects didn't maintain the lost weight. The diet approach method tried was sequently the fasting 1 meal or the more in a day(23.61%), exercise(21.92%), restricting calories(15.85%) etc. The source of diet was orderly newspaper or magazine(30.86%), friends or relatives or family(28.86%) etc. The group who recognized the self body shape was normal or a little fat had higher self notion of health than other groups.
This study examined the effect of brand experience and personality on customer-brand relationships, brand attachment, and brand loyalty in domestic and global coffee shop brands. By comparing inter-structural relationships among factors between domestic brands and global brands, this study also provided strategic implications and directions for the effective marketing strategy of coffee shop brands. Data were collected through a self-administered survey of the consumers aged 20's to early 40's who used domestic and global brand coffee shops in Seoul and Kyunggi between August 1, 2014 and August 30, 2014. 500 surveys were distributed and 495 surveys were returned. Among them, data from 493 surveys were used for the analysis. The results from this study are as follows. First, it was found that the Think of experiences in coffee shop had a greater effect on consumer-brand relationship than the Sense-Feel. Second, the Sophistication of brand personality in coffee shop had a greater effect on consumer-brand relationship than the Excitement. Third, only Think of experience in coffee shop significantly impacted brand attachment, but Sense-Feel did not impact brand attachment. Fourth, only Excitement of brand personality in coffee shop significantly impacted brand attachment, but Sophistication did notact on brand attachment. Fifth, the consumer-brand relationship had a greater effect on brand loyalty than did brand attachment. Finally, the differences from a comparison analysis of domestic brands and global brands of coffee shop are as follows. In domestic brands, only the Sophistication of brand personality in coffee shop significantly impacted consumer-brand relationship. In global brands, the brand personality in coffee shop did not impact on consumer-brand relationship.
The purpose of this study was to examine the use of dentifrice among children in a bid to provide information on dentifrice including its function to children's parents, the major consumers of dentifrice for child. And it's additionally meant to offer useful information on the production of toothpaste for kid. The subjects in this study were parents with children, who dwelled in Seoul and Gyeonggi province. After a self-administered survey was conducted for about three months from May to July 2007 to gather data on their use of dentifrice for child, a comparative analysis was implemented. The findings of the study were as follows: 1. Concerning the state of the use of their children's dentifrice, the largest number of the children(58.1%) started to use toothpaste at the age of one or down, and the most common first toothpaste they used was dentifrice for preschooler(86.8%). As for whether to use fluoride-containing toothpaste, the largest group of the parents(58.1%) gave an affirmative answer. Regarding the form of the toothpaste in use, the majority of their children used toothpaste of cream type(86.2%). The greatest group of the children used the amount of toothpaste that was as large as the three-tenths or four-tenths of the bristles(35.9%). 2. As for the state of the use of dentifrice for kid, the greatest group used that kind of toothpaste(81.4%). In the event of those who didn't use it, the last time when their children used toothpaste for kid was when they were at the western age of 3(33.9%) and 4(33.9%). Concerning the reason why they replaced toothpaste for kid with one for adult, the largest group of the parents did that on their own judgment(58.1%). As to the right time for replacing toothpaste for kid by one for adult, the greatest group considered it advisable for children to start using toothpaste for adult at the western age of 4 or 5(43.2%). 3. In relation to the state of the purchase of toothpaste for their children, the largest group was under the moderate influence of the children when they bought it(45.2%). The greatest group attached importance to the ingredients of toothpaste(41.6%), and the most preferred ingredient was fluoride(56.3%). 53.0 percent took consideration on the content of ingredients or the instructions. 4. In regard to priorities for the choice of toothpaste for their children, they gave top priority to brand(16.7%), followed by quality(14.6%) and ingredients(13.5%). The age of the parents made a statistically significant difference to the children's age when they started using toothpaste, and how they started to do that and whether they used fluoride-containing toothpaste were statistically significantly different according to that as well(p < 0.01). In regard to the impact of the occupation of the parents, the use of fluoride-containing toothpaste was more common among the stay-at-home mothers(p < 0.01).
The shortage of dental hygienists has been a long-standing problem in Korea. Small-scaled dental clinics suffer from a lack of dental hygienists, who seem to prefer working at large-scaled dental clinics. The purpose of this study was to confirm the differences in the working conditions according to the scales of dental clinics. We collected the working information registered via job advertisements through the web-sites of Korean Dental Hygienists Association, Dental Jobs, and Nurse Jobs from July to August 2016. The results were as follows: 96.7% of the advertisements wanted regular workers, while the proportion of part-time workers was the highest (34.8%) in the group with less than 3 employees. The average workdays per week was $5.32{\pm}0.55$ days, and the group with less than 3 employees had significantly longer workdays than the other groups. The daily working time was $8.99{\pm}0.44$ hours, and there was no difference among the groups. Night overtime hours were needed by 54.4%, 45.0%, and 31.3% of the groups with of the groups with 4~7 employees, more than 8 employees, and less than 3 employees, respectively. Information regarding annual leave (60.5%), monthly leave (63.9%), half a day off (32.4%) and vacations (43.1%) were presented in the job advertisements, and these proportions were significantly higher by the group with more than 8 employees. Information on overtime pay (14.4%), night-work pay (13.4%), incentives (34.1%), lunches (60.2%), vacation bonuses (33.8%), and self-development (20.4%) were presented in job advertisements. The group with 4~7 employees had significantly higher proportions in severance pay, vacation bonuses, self-development, and major national insurance. It is necessary to consider the improvement of working conditions, diversity of working styles, and welfare of dental hygienists, and it is suggested that small dental clinics provide more precise working conditions.
This study was conducted to investigate the types, socio-demographic characteristics, use patterns, and visit motivations of visitors to "Jangseong Pyunbaek Healing Forest (JPHF)." Data were collected from 216 samples selected by convenient sampling method using questionnaire survey between October and November 2016. Data obtained from 476 respondents of the same type of survey during the same period were partly used to examine the types of activities of visitors to the study site. The results showed that 77.0 percent of respondents were categorized as "visitor for recreation" (VR: Those who visit JPHF for the purpose of general recreational experiences such as appreciating landscapes, tourism, social interactions rather than better health or healing). On the other hand, only 10.3 percent of respondents were "visitor for healing" (VH: Those who visit JPHF for the purpose of better health or physical and mental healing). VH showed a higher level of visit experiences to JPHF than VR and tended to expect JPHF as "the place to enjoy calm and natural experiences" more than VR did. VH tended to visit JPHF "alone" more, and their group size was also smaller than VR. About 26.8 percent of all respondents were not aware that JPHF was the "healing forest," and VH showed higher awareness of it than VR. About 66.8% of respondents assessed the managerial and operating states of JPHF positively while only 6.2% assessed them negatively. There was no difference in the assessment of managerial and operating states between VR and VH. The visitors of JPHF showed the markedly high use in main forest roads (use rate: 47.4%) and "Healing Field" (use rate: 59.2%) of JPHF. Regarding the visit motivations to JPHF, VH showed higher importance on "quietude," "health," and "tension release" than VR while VR rated higher importance on "social interaction." The paper discussed the managerial implications, findings, limitations and further studies.
This study was conducted to develop a plan for emotional labor of clinical dental hygienists by understanding the factors affecting their emotional labor. Self-administered questionnaires were distributed to 293 dental hygienists who worked in dental hospitals and clinics located in Busan and Gyeongsangnam-do from June to September in 2013. The questionnaire items used in this study consisted of 5 items of general characteristics, 2 items of characteristics related to turnover, and 9 items of emotional labor. t-test, ANOVA, and multiple regression analysis were performed using the SPSS Statistics ver. 17.0 for Windows program for data analysis. The average level of the study subjects was $69.85{\pm}12.82$. With regard to the emotional labor according to the general characteristics, the frequency of emotional expressions (p<0.037) showed significant differences in the marital status. The workplace showed significant differences in the frequency of emotional expressions (p<0.001), attentiveness of emotional expressions (p<0.002), emotional dissonance (p<0.032), and total (p<0.001). The turnover intentions showed significant differences in the frequency of emotional expressions (p<0.001), attentiveness of emotional expressions (p<0.001), emotional dissonance (p<0.001), and total (p<0.001). As a factor affecting emotional labor, turnover intention (p<0.001) and workplace (p<0.001) had an effect on emotional labor. The results showed that turnover intention and workplace were associated with the emotional labor of dental hygienists. There is a need to develop an emotional education program to make it possible to effectively manage emotional labor of dental hygienists for providing high-quality medical services of dental institutions.
The purpose of this study was to investigate the differences in health belief related to vaccination among dental hygiene students, identify the factors affecting hepatitis B vaccination, and provide basic data for health education concerning hepatitis B prevention. Hepatitis B vaccination was positively correlated (r=0.179, p=0.003) with seriousness of believing that hepatitis B would affect psychological, physical, and social activities and negative correlation (r=-0.183, p=0.002) was also found between perceived barriers related to hepatitis B. As for the factors affecting hepatitis B vaccination, juniors were 0.39 times (95% confidence interval [CI]=0.21, 0.71) more likely to have it affected than seniors and those who were more serious, and who had greater barriers were more likely to have it affected-1.80 times (95% CI=1.29, 2.52), 0.61 times (95% CI=0.41, 0.90) respectively (p<0.05). It is therefore urgent to develop a program within a curriculum that can begin with freshmen along with specific education with the objective of obliging dental hygiene students to receive hepatitis B vaccination. It is also necessary to provide consecutive sessions of education rather than one-time education and to develop a specific plan for obligatory hepatitis B vaccination through correct and thorough education.
Kim, Shin-Hye;Hur, Hae Young;Lee, Kyu Hyoung;Moon, Ja Young;Chae, Kyu Young
Clinical and Experimental Pediatrics
/
v.50
no.8
/
pp.746-752
/
2007
Purpose : Total hydroperoxide (TH), free radical-mediated oxidation product can be used as a measure of free radical injury. The aim of the present study was to see if preterm newborns are at increased risk for oxidative stress compared with term newborns, and to determine whether oxidative stress during postnatal first 1 week is associated with clinical outcomes in preterm infants. Methods : Serum TH levels of preterm infants (n=39) were compared with those of term infants (n=24) on the postnatal day 1. Among the preterm infants, serum TH levels of uncomplicated group (n=23) were also compared with those of complicated group (n=16) who developed oxygen radical related diseases on the postnatal day 1 and 7. Retrospective analysis was performed to find out risk factors for oxygen radical injuries based on birth history, laboratory data, neuroimaging findings and clinical progress in two preterm groups. Results : Serum TH levels on postnatal day 1 were higher in the preterm infant group than the term infant group. Serum TH levels on postnatal day 1 in the complicated preterm infant group were significantly higher compared with uncomplicated group, but there was no significant difference in serum TH levels on postnatal day 7. Also, there was no significant difference in serum TH levels between uncomplicated preterm infants and term infants. Serum TH level on postnatal day 1 was independently associated with higher morbidity after adjusting for gestational age, Apgar score (5 min), arterial blood gas analysis. Conclusion : Complicated preterm newborns are at increased risk for oxidative stress compared with uncomplicated newborns and term newborns. Oxidative injury during the prenatal or postnatal day 1 is associated with adverse outcomes in preterm infants. Elevated TH levels on postnatal day 1 may have a value to predict clinical outcomes in preterm infants.
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