• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.198-206
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• 1981

A field research was conducted to evaluate the effects of inoculation of Rhizobium meliloti and lime application on the early growth of alfalfa, var. Luna, in an acid soil. Alfalfa seeds were inoculated by lime-peat pelleting method with $A_3$ strain isolated from alfalfa roots. Both inoculated and noninoculated seeds were sown in acid soil (pH 5.5) with three levels of lime application (1, 3, 6 ton/ha) on mid-April. Inoculated seeds produced many effective nodules in early stage and increased nitrogen and chlorophyll contents of leaf, consequently stimulating alfalfa growth. Pseudo nodules were found in noninoculated, while they were seldom formed in inoculated. Lime application increased soil pH, plant height, root length, and shoot and root weight. Liming effect on nodulation was significant at first cut, but no longer after then. Both inoculation and liming increased the forage yield of alfalfa. Inoculation and liming of 6 ton/ha together produced the highest yield. As compared to control plot of nitrogen application, the effect of seed inoculation only could be equal to about 120 kg/ha nitrogen application. The total nitrogen contents of forage were little affected by number or weight of nodules and amounts of lime application.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.469-480
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• 2015

The study was conducted on the effects of time of defoliation on fruit quality of pear (Pyrus pyrifolia Nakai) trees, managing with low-pesticides, and regrowth of stem cuttings in vitro. Treatments included for 40% of uniform defoliation at early-August, end-August, and early-September, as well as control (no defoliation). Defoliation at early-September and control increased growth of water sprouts as well as concentrations of carbohydrates, total nitrogen, and free sugar in one-year old shoots. Defoliation at early-September and control increased fruit yield and mean fruit weight, with high soluble solids content and fruit surface color of $a^*$ observed for both defoliation at end-August and early-September. Defoliation at early-August increased rates of electrolyte leakage in stem cuttings at $-18^{\circ}C$ in vitro. There were no significantly different for germination rates of the cuttings between the treatments at -18 and $-21^{\circ}C$ in vitro, with the highest germination of the cuttings observed for defoliation at early-September and control at $-27^{\circ}C$. Therefore, orchard management should be performed to be minimized for defoliation of the spur leaves until end-August, causing from precipitation and pests.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.198-204
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• 1994

This study was carried out to investigate the auxin(IAA, IBA, NAA) treatment and the effect of shading rate in Asarum sieboldii. The results obtained were summerized as follows: By the soaking treatment of auxins to the cutted rhizome enhanced root growth and plant weight. By the increment of shading rate, plant growth was much better compare to the control. Leaf fallen times appeared about 20 days more earlier at plain area then the alpain area. Root yield was much higher by the treatment of shading then the conventional cultivation so it seem to be the useful for large scale cultivations of A. sieboldii.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.833-847
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• 2016

Drought stress is a major agricultural limitation to crop productivity worldwide, especially by which leafy vegetables, plant leaves eaten as vegetable, could be more lethal. The study was carried out to know the effect of drought tolerance plant growth promoting bacteria (PGPB) on water stress of kale seedlings. A total of 146 morphologically distinct bacterial colonies were isolated from bulk soil and rhizosphere soil of leafy vegetables and screened for plant growth promoting microbioassay in greenhouse. Out of them the isolate SB19 significantly promoted the growth of kale seedlings in increasement of about 42% of plant height (14.1 cm), 148% of leaf area ($19.0cm^2$) and 138% of shoot fresh weight (1662.5 mg) attained by the bacterially treated plants compared to distilled water treated control (9.9 cm, $7.7cm^2$, 698.8 mg). Shoot water content of SB19 treated kale seedlings (1393.8 mg) was also increased about 152% compared with control (552.5 mg). The SB19 isolated from bulk soil of kale plant in Iksan, Korea, was identified as species of Bacillus based on 16S rRNA gene sequencing analysis. We evaluated the effect of drought tolerance by the Bacillus sp. SB19 on kale seedlings at 7th and 14th days following the onset of the water stress and watering was only at 7th day in the middle of test. In the survey of 7th and 14th day, there were mitigation effect of drought stress in kale seedlings treated with $10^6$ and $10^7cell\;mL^{-1}$ of SB19 compared to distilled water treated control. Especially, there were more effective mitigation of drought damage in kale seedlings treated with $10^7cell\;mL^{-1}$ than $10^6cell\;mL^{-1}$. Further, although drought injury of bacterially treated kale seedlings were not improved at 14th day compared with 7th day, drought injury of $10^7cell\;mL^{-1}$ of SB19 treated kale seedlings were not happen rapidly but developed over a longer period of time than $10^6cell\;mL^{-1}$ of SB19 or control. The diffidence of results might be caused by the concentration of bacterial suspension. This study suggests that beneficial plant-microbe interaction could be a important role of enhancement of water availability and also provide a good method for improving quality of leafy vegetables under water stress conditions.

• Korean Journal of Organic Agriculture
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• v.26 no.2
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• pp.217-232
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• 2018

Drought is a major obstacle to high agricultural productivity, worldwide. In drought, it is usually presented by the simultaneous action of high temperature and drying. Also there are negative effects of plant growth under drying conditions. In this study, the effect of Bacillus velezensis YP2 on plant growth-promotion and soil drying stress tolerance of kale plants, Brassica oleracea var. alboglabra Bailey, were investigated under two different conditions; greenhouse and field environments. Root colonization ability of B. velezensis YP2 was also analysed by using plating culture method. As a result of the greenhouse test, the YP2 strain significantly promoted the growth of kale seedlings in increasement of 26.7% of plant height and 142.2% of shoot fresh weight compared to control. B. velezensis YP2 have the mitigation effect of drying injury of kale by decreasing of 39.4% compared to control. In the field test, B. velezensis YP2 strain was also found to be effective for plant growth-promotion and mitigation of drying stress injury on kale plants. Especially, relative water contents (RWC; %) were higher in B. velezensis YP2 treated kales than in control at 7, 10, 14 day after non-watering. The root colonization ability of YP2 strain was continued at least for 21 days after soil drenching treatment of B. velezensis YP2. Our result suggested that enhancement of plant growth and drying injury reduction of kale plants were involved in kale root colonization by B. velezensis YP2, which might be contributed to increasing water availability of plants. Consequentially, the use of B. velezensis YP2 might be a beneficial influence for improving productivity of kale plants under drying stress conditions.

• Ecology and Resilient Infrastructure
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• v.5 no.3
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• pp.163-173
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• 2018

The biopolymer (BP) used in this study is mainly composed of xanthan gum and ${\beta}$-glucan derived from microorganism and has been introduced as a novel material for soil stabilization. However, the broad applicability of BP has been suggested in the field of geotechnical engineering while little information is available about the effects of BP on the vegetation. The goal of this study is to find the BP effects on the growth of Camelina sativa L. (Camelina) under drought condition. For more thorough evaluation of BP effects on the plant growth, we examined not only morphological but also physiological traits and gene expression patterns. After 25 days of drought treatment from germination in the soil amended with 0, 0.25, 0.5, and 1% BP, we observed that the BP concentration was strongly correlated the growth of Camelina. When plants were grown under drought stress, Camelina in 0.5% BP mixture showed better physiological parameters of the leaf stomatal conductance, electrolyte leakage and relative water content compared to those in control soil without BP. Plant recovery rate after re-watering was higher and the development of lateral root was lower in BP amended soil. RNA expression of Camelina leaf treated with/without drought for 7 and 10 days showed that aquaporin genes transporting solutes at bio-membrane, CsPIP1;4, 2;1, 2;6 and TIP1;2, 2;1, were induced more in the plants with BP amendment and drought treatment. These results suggest that the soil amended with BP has a positive effect on the transport of nutrients and waters into Camelina by improving water retention in soil under drought condition.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.893-899
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• 2004

Method for sample preparation and quantitative analysis of 19 permitted and non-permitted synthetic colors in foods was developed based on reversed-phase ion-pairing high performance liquid chromatography. For color extraction of samples, deionized water was added, and pH was appropriately adjusted with 1% ammonia water. Any undissolved matters were extracted with 50% ethanol or 70% methanol. Lipid in snacks was first removed using n-hexane with centrifugation, water was added to extract colors, followed by clean-up and concentration using Sep-Pak $C_{18}$ cartridge. Recovery efficiencies at known concentrations of 19 standard food colors spiked into foods were in 90.3-97.9% range far soft drink, 79.2-101.9% for candy, 84.1-103.4% for jelly, 86.4-100.8% for chewing gum, 83.5-103.4% for ice cream, and 78.5-95.6% for snack.

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.7-13
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• 1997

Tomatoes are flooded differently 0, 5, 10 and 15 ㎝, according to the developing stages such as transplanting stage under the condition of green house. Along with this, they are treated according to the time condition such as 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. As the depth of flooding got deeper and the hours got longer, plant height, number of leaves, shoot and root decreased significantly. Flowering was possible for 24 hours in the flooding of 0 ㎝, for 6 hours in $5{\sim}10$ ㎝, but not possible after 6 hours in 15 ㎝. Without regard to the depth of flooding, adventitious root came into being before or after 48 hours of the treatment. Root activity diminished gradually as hours of treatment went by, but diminished rapidly over the depth of 5 ㎝. Chlorophyll content decreased similarly as in the case of root activity. Diffusion resistance of stomata cell increased as hours of treatment passed and depth increased. Photosynthesis and respiration diminished according as the hours and depth of treatment increased. Respiration diminished a little gradually but photosynthesis weakened greatly as the depth of treatment became greater and after 48 hours of treatment. Diseases occurred remarkably in proportion to the depth of treatment and the increase of hours. The possibility of preventing by means of insecticide treatment showed the same tendency as in the seedling stage. But its effect was not significant. After 120 hours yields could not be expected because tomatoes died without regard to the depth of flooding. Instead of the depth, numbers of fruits per plant decrease of individuals or variation of average weight of a fruit was recognized. Especially average weight increased in accordance with the increase of the depth. There was positive correlation between all the characters, such as plant height, number of leaves, fresh weight, chlorophyll content, root activity and yield traits, but negative correlation between these and epinastic curvature, diffusion resistance and adventitious root.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.150-156
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• 2008

The influences of night break by costly artificial light sources were investigated on the photo-morphogenesis and growth of leafy perilla (Perilla ocymoides L.). The irradiation of red, blue, and three-colored light for night break significantly increased the stem length and stem diameter compared to dark. Three-colored light gave the highest fresh and dry weight of stem, followed by red and blue light. Floral induction was suppressed up to 100 days after the night break, by red and three-colored light, but the plants grown under the dark or treated with blue light showed 85% and 31% flowering rate, respectively. The time needed for floral induction after night break was 60 days in dark and 80 days in blue light. The number of leaf, leaf area, and fresh weight per plant were the highest in red and three-colored light night break, followed by blue light and dark. The photosynthetic rate observed 80 days after night break was the highest in red light, followed by blue and three-colored light. A low light compensation point of $20\;{\mu}mol\;m^{-2}{\cdot}s^{-1}$ was observed in three-colored light, while red and blue light tended to show higher measurements.