Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Enhancement of Plant Growth and Drying Stress Tolerance by Bacillus velezensis YP2 Colonizing Kale Root Endosphere

Bacillus velezensis YP2 균주의 근권 정착에 의한 케일의 생육 촉진 및 건조 스트레스 완화 효과

  • 김다연 (농촌진흥청 국립농업과학원) ;
  • 한지희 (농촌진흥청 국립농업과학원) ;
  • 김정준 (농촌진흥청 국립농업과학원) ;
  • 이상엽 (농촌진흥청 국립농업과학원)
  • Received : 2017.10.31
  • Accepted : 2018.05.24
  • Published : 2018.05.31
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Abstract

Drought is a major obstacle to high agricultural productivity, worldwide. In drought, it is usually presented by the simultaneous action of high temperature and drying. Also there are negative effects of plant growth under drying conditions. In this study, the effect of Bacillus velezensis YP2 on plant growth-promotion and soil drying stress tolerance of kale plants, Brassica oleracea var. alboglabra Bailey, were investigated under two different conditions; greenhouse and field environments. Root colonization ability of B. velezensis YP2 was also analysed by using plating culture method. As a result of the greenhouse test, the YP2 strain significantly promoted the growth of kale seedlings in increasement of 26.7% of plant height and 142.2% of shoot fresh weight compared to control. B. velezensis YP2 have the mitigation effect of drying injury of kale by decreasing of 39.4% compared to control. In the field test, B. velezensis YP2 strain was also found to be effective for plant growth-promotion and mitigation of drying stress injury on kale plants. Especially, relative water contents (RWC; %) were higher in B. velezensis YP2 treated kales than in control at 7, 10, 14 day after non-watering. The root colonization ability of YP2 strain was continued at least for 21 days after soil drenching treatment of B. velezensis YP2. Our result suggested that enhancement of plant growth and drying injury reduction of kale plants were involved in kale root colonization by B. velezensis YP2, which might be contributed to increasing water availability of plants. Consequentially, the use of B. velezensis YP2 might be a beneficial influence for improving productivity of kale plants under drying stress conditions.

전 세계적인 지구 온난화로 인한 가뭄은 농작물의 생산성을 저해하는 주요 원인 중 하나이며, 고온과 건조가 복합적으로 작용하여 식물 생장을 감소시킨다. 본 연구에서는 Bacillus velezensis YP2 균주의 식물 생육촉진 및 건조 스트레스 내성 증진 효과를 온실과 시설하우스 포장에서 조사하였다. 또한 B. velezensis YP2 균주의 처리 전과 후 케일 근권과 뿌리에서 배양법에 의한 상대 정량 방법으로 B. velezensis YP2 균주의 근권 및 뿌리 정착능을 분석하였다. 온실 검정 결과 YP2 균주 처리구에서는 무처리구와 비교하여 케일 유묘의 초장 26.7% 및 지상부 생체중 142.2% 증가시키는 효과가 있었다. 또한 B. velezensis YP2 처리구에서는 무처리구와 비교하여 39.4%의 건조 피해 경감 효과가 있었다. 시설하우스 포장 검정 결과에서도 B. velezensis YP2 균주 처리에 의한 케일의 생장촉진 효과와 건조 스트레스 내성 증진 효과가 있었으며, B. velezensis YP2 처리구에서 케일 잎의 상대수분함량이 무처리구와 비교하여 7, 10, 14일에 모두 높은 것으로 나타났다. B. velezensis YP2 균주의 뿌리 정착능 분석 결과, 균주 처리 21일까지 케일 근권 및 뿌리 균밀도가 무처리구와 비교하여 B. velezensis YP2 처리구에서 유의하게 높은 것으로 나타났다. 따라서 균주 처리 후 최소한 21일이 경과할 때까지 B. velezensis YP2 균주가 케일 근권과 뿌리에 정착하여 식물과 상호작용함으로서 생육을 촉진하고 식물의 물 이용률을 증가시켜 건조 스트레스 내성을 증진하는 데 관련이 있을 것으로 판단된다. 본 연구 결과를 통하여 B. velezensis YP2 균주는 가뭄으로 인한 건조한 토양 조건에서 작물 생산성을 향상시키는 가능성이 있는 유용한 미생물로 이용할 수 있을 것으로 생각된다.

Keywords

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