• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.581-585
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• 1986

A growth experiment of the Israeli strain of common carp (Cyprinus carpio) under varying dissolved oxygen levels in the recirculating water system was conducted at the Fish Culture Experiment Station of the National Fisheries University of Pusan from August 28, 1985 to September 17, 1985. Five tanks with a capacity of $5m^3$ of water each were used under the same condition of water parameters except for dissolved oxygen levels which were designed to maintain at 2.0, 2.5, 3.0, 3.5 and 4.0 mg/l ranges. The weight of fish in the beginning was about 300g and each tank was stocked with 200kg of fish. DO level of 3.5mg/l was found to be the best level with a feed coefficient of 1.57 and a daily growth rate of $1.411\%$ whereas 4.mg/l showed a slightly decreased performance of 1.63 and $1.365\%$ respectively. The amounts of feed consumed in 3.5 and 4.0mg/l DO levels were almost the same. Below 3.0mg/l DO levels the growth rate markedly decreased. Furthermore, in 2.0 and 2.5 mg/l groups, the fish did not accept feed vigorously and after feeding the fish usually concentrated around the inflow point showing oxygen deficiency response, The experiment indicates that the DO range of 3.5 to 4.0mg/l is the optimum level for the best growth at $27.5^{\circ}C$. DO concentration above these levels is considered a waste of energy resulting in uneconomical performance, and on the other hand, below these levels, the carp certainly shows a poor growth performance.

• Korean Journal of Ichthyology
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• v.18 no.1
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• pp.27-35
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• 2006

During spawning season of female in a Korean oily bitterling, Acheilognathus koreensis, the ventral region near the base of the pectoral fin becomes to be protruded outward of body and enlarged. This ventral process consists of both organs as rectum (vent) and inner ovipositor. The rectum consists of mucosa, lamina propria-submucosa, muscularis, and squamous epithelial layer (peritoneum=serosa) surrounding them. The mucosa contains numerous mucous cells meaning acid mucopolysaccharides in nature. The inner ovipositor is similar to that of the rectum, but the mucosa have no mucous cell, unlike that of the rectum. Whereas, the outer ovipositor has a straight and long tube which are not connected with the ventral process any more. The outer ovipositor was similar to the structure of the inner ovipositor in the ventral process. However, the outer ovipositor has no muscularis, and consists of three layers: mucosa, lamina propria-submucoa, and squamous epithelia. The outer ovipositor without the muscularis seems serves as a tube that eggs discharged from the outer ovipositor allow to send inside mussel, unlike that of the inner ovipositor performing rhythmic contractions of the layers of the muscularies for propelling to the matured oocytes to the outer ovipositor.

• Korean Journal of Ichthyology
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• v.25 no.2
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• pp.135-140
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• 2013

Microphysogobio koreensis, an endemic Korean freshwater fishes belonging to Cyprinidae, is protected as a 2nd grade of endangered fishes by the ministry of the Environment. For this species, the study on the spawning microhabitat including structure of streambed and spawning grounds was carried out. The streambed that this species mainly lives consists of boulders, cobbles, and pebbles, the so-called Bb type, with a depth of 0.3 to 1.0 m and a rapid velocity of $0.5{\pm}0.2$ m/sec. But the main spawning grounds where the spawning is actually occurring, is relatively shallower, $0.4{\pm}0.1$ (0.4~0.6) m depth, and faster, $0.8{\pm}0.1$ (0.6~0.9) m/sec velocity, whereas the region that the fertilized eggs are attached are rather slowest to 0.4m/sec. The spawning starts at a water temperature of $20^{\circ}C$ in earlier May, laying their eggs on the cobbles' or boulders' surface or in between them. In particular, with a wide range of streambed structures, natural spawning grounds' environments may let the fertilized eggs not be swept from rapid current and secure efficient dissolved oxygen. The individuals, however, do not appear at the grounds any more after the spawning season, and it is expected that they may move into deeper water such as Lakes or lower streams.

• The Korean Journal of Zoology
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• v.22 no.1
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• pp.1-10
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• 1979

From the experimental results of cellulose acetate membrane electrophoresis we concluded the followings in explaining the LDH isozyme patterns found in the retina and cerebrum of vertebrata. Lactate dehydrogenase of the retina and cerebrum of both Carassinus carassinus and Cyprinus carpio was found to have one diffused band located between $LDH_2$ and $LDH_1$. LDH isozyme patterns of heart, pectoral muscle, liver and stomach of the Cyprinus carpio had the same diffused band in all organs. LDH isozyme patterns of the cerebrum of Hynobius leechii and Rana nigromaculata were observed to be different, in Hynobius leeichi a single band moved to the negative pole and two bands of $LDH_5$ and $LDH_4$ were obtained in the Rana nigromaculata. The retina and cerebrum of Natrix tigrina lateralis were observed as one band but amyda maakii had different LDH isozymes of the retina and cerebrum. The retina of Amyda maakii had five distinct LDH isozyme bands which had decreasing activity in the order of $LDH_5, LDH_4, LDH_3, LDH_2 and LDH_1$. The cerebrum of Amyda maakii had one band like Natrix tigrina lateralis but it moved to the negative pole. LDH isozymes in the retina and cerebrum of Gallus gallus domesticus and Melopsittacus undulatus showed one band. Five characteristic LDH isozyme bands were obtained from the retina of mammals, Oryctolagus cuniclus, Canis familiaris, Sus scrofa bos taurus and in the cerebrum of mouse, albino rat, Rhinolophus ferrum-equinum kokai.

• Journal of Life Science
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• v.8 no.3
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• pp.348-351
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• 1998

Common DNA fragments of the ${\beta}$-globin gene were observed from six races of the adult common carp: Hybrid-Yamato, Japanese wild type, Mirror, Suwa-Yamato, Scale German, and Saku-Yamato. Chromosomal DNAs isolated from the above six races were digested with restriction endonucleased EcoRI and PstI. The digested fragments were transferred onto nitrocellulose filter and hybridized with a probe of carp ${\beta}$-globin cDNA. Molecular sizes of the hybridized DNA fragments digested with EcoRI were 3.6Kb(Kilo base), 4.3Kb and 15Kb in Hybrid-Yamato, Japanese wild type, Mirror, Scale German and Saku-Yamato carp DNAs. In Scale German and Saku-Yamato carp DNAs, two and one more hybridized DNA fragments were observed, respectively. Molecular sizes of the hybridized DNA fragments digested with PstI were 2.2Kb, 6.5Kb, 7.8Kb and 9.2Kb in Hybrid-Yamato, 2.2Kb, 6.5Kb and 9.2Kb in Japanese wild type, 2.2Kb, 6.5Kb, 7.8Kb, and 13Kb in Mirror, 2,2Kb, 5,5Kb, 6.5Kb, 7.8Kb, 9.2Kb and13Kb in Scale German, and 2.2Kb, 5.5Kb, 6.5Kb, 9.2Kb and Saku-Yamato carp DNA. Therefore, depending on carps, three to six DNA fragments were hybridized with ${\beta}$-globin gene probe. Thus it indicated polymorphysm in the globin gene family of carp.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.320-324
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• 2010

We investigated the proper feeding frequency and satiation rate for the growth of red- and white-colored carp fingerlings in a feeding experiment. Triplicate groups of fish averaging 6.3 g were fed diets at four feeding frequencies (1, 2, 3, or 4 meals a day) and two satiation rates (satiation and 80% satiation) for 6 weeks. The survival of the fish was not affected by either the feeding frequency or the satiation rate. The weight gain, feed efficiency, and protein efficiency ratio of fish were affected by the feeding frequency and satiation rate, and increased significantly with both. The feed efficiency and protein efficiency ratio with satiation feeding of fish fed four meals a day did not differ from those of fish fed three meals a day. The daily feed intake was affected by the feeding frequency and satiation rate. The moisture, crude lipid, and ash contents of whole body were affected by the feeding frequency. Moisture and crude lipid contents were affected by feeding satiation. Based on these results, we conclude that the proper feeding frequency and satiation rate for the optimum growth of red- and white-colored carp fingerlings is four meals a day with satiation feeding.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.3
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• pp.193-200
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• 2008

We experimentally investigated effects of four concentrations (4, 6, 8, and 10%) each of dietary Spirulina and Chiorella, as well as four concentrations of dietary astaxanthin (40, 60, 80, and 100 ppm in Carophyll Pink), on the body color of red- and white-colored carp, Cyprinus carpio. The total carotenoid concentration in the skin tissue of the red parts of the carp fed dietary Spirulina, Chiorella, and astaxanthin increased up to the second week of the experiment but decreased thereafter. The redness value of the Spirulina-and Chiorella-treated fish decreased up to the sixth week of the experiment and increased thereafter. However, the redness value of the astaxanthin-treated fish consistently increased, beginning in the second week. Of the three agents tested, astaxanthin in Carophyll Pink was the most effective at enhancing the redness of both red- and white-colored carp. The redness value of the fish did not statistically differ among the various concentrations of astaxanthin. Therefore, dietary supplementation with 40ppm astaxanthin would be the most economical method for enhancing the redness of red- and white-colored carp fingerlings.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.484-489
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• 2011

This study examined whether N-acetylcysteine (NAC), a glutathione precursor, could attenuate toxic effects of three fish anti-parasitic agents, trichlorfon, hydrogen peroxide ($H_2O_2$) and formalin, all of which are known to exert side effects through free radical production. Common carp Cyprinus carpio were fed with NAC (approx. 50 mg/kg/day) for 3 consecutive days prior to anti-parasite bathing for a 24 hr period. Mortality rates were examined during this 24 hr bathing period, and selected hematological and biochemical parameters were also assessed at the termination of anti-parasite exposure. The mortality rates and plasma glucose elevations caused by all three anti-parasitics were significantly reduced by NAC pretreatment. Trichlorfon, but not $H_2O_2$ or formalin, elevated plasma levels of aspartatetransaminase (AST) and alanine-transaminase (ALT), and these elevations were attenuated by NAC. There was no change in hematocrit values in any treatment. The results provide evidence for the attenuating effects of NAC against toxicity caused by anti-parasite agents that act through free radical-producing properties. The results found in this study also suggest that NAC may be administered to fish to minimize toxicity in fish parasiticide use.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.4
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• pp.337-342
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• 2012

A feeding trial was conducted to investigate effect of three paprika levels (0%, 5% and 10%) with two lipid levels (4% and 10%) in diets on growth performance and skin coloration of red- and white-colored fancy carp, Cyprinus carpio var. koi. Two replicate groups (an average body weight of $4.3{\pm}0.2g$) of fish fed one of eight experimental diets for 8 weeks. Survival, weight gain and feed efficiency of the fish were not significantly affected by both dietary paprika and lipid levels (P>0.05). After 8 weeks feeding period, the values of $a^*$, $L^*$ and $b^*$ of fish skin were significantly changed by dietary paprika level (P<0.05) but not lipid level. The $a^*$ value of fish fed the diets containing paprika was higher than that of fish fed control diet (P<0.05). Additional inclusion of 50-100 ppm canthaxanthin in diets containing 5% and 10% paprika did not improve $a^*$ value of fish skin. Based on the results of this study, it can be concluded that dietary inclusion of 5% paprika powder could increase the redness of red- and white-colored fancy carp fingerling.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.745-749
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• 2017

We investigated the effects of different dietary lipid levels (7% or 14%) and feeding frequency (1-4 meals per day) on the growth performance and body composition of juvenile fancy carp Cyprinus carpio var. koi. Three replicate groups of fish (initial mean weight, 11.7 g) were fed to visual satiety with sinking dry pellet diets for 8 weeks. Neither feeding frequency nor lipid level affected fish survival. Weight gain was affected by feeding frequency but not dietary lipid level. Weight gain significantly increased with increased feeding frequency. Feed efficiency and protein efficiency ratio were affected by feeding frequency, but not dietary lipid level. Daily feed intake was affected by feeding frequency and dietary lipid level. Whole body moisture, crude lipid and ash contents were affected by feeding frequency, but not dietary lipid level. Based on these results, we conclude that the appropriate feeding frequency for the optimum growth of fancy carp fingerlings is four meals per day using sinking dry pellet.