• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.11
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• pp.1552-1563
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• 2016

To examine the cognitive function of onion (Allium cepa L.) beverages (odourless and fortified), we analyzed in vitro neuronal cell protection against $H_2O_2$-induced cytotoxicity and performed in vivo tests on amyloid beta ($A{\beta}$)-induced cognitive dysfunction. Cellular oxidative stress and cell viability were evaluated by DCF-DA assay and MTT assay. These results show that fortified beverage resulted in better neuronal cell protection than odourless beverage at lower concentration ($0{\sim}100{\mu}g/mL$). Fortified beverage also showed more excellent acetylcholinesterase (AChE) inhibitory activity ($IC_{50}$: 4.20 mg/mL) than odourless beverage. The cognitive functions of odourless beverage and fortified beverage in $A{\beta}$-induced neurotoxicity were assessed by Y-maze, passive avoidance, and Morris water maze tests. The results show improved cognitive function in both groups treated with beverages. After in vivo tests, cholinergic activities were determined based on AChE inhibition and acetylcholine levels, and antioxidant activities were measured as SOD, oxidized glutathione (GSH)/total GSH ratio, and MDA levels in mouse brain tissue. In a Q-TOF UPLC/MS system, main compounds were analyzed as follows: odourless beverage (five types of sugars and three types of phenolics) and fortified beverages (six types of phenolics and two types of steroidal saponins).

• The KIPS Transactions:PartB
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• v.10B no.3
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• pp.281-286
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• 2003

A large number of features are collected for problem solving in real life, but to utilize ail the features collected would be difficult. It is not so easy to collect of correct data about all features. In case it takes advantage of all collected data to learn, complicated learning model is created and good performance result can't get. Also exist interrelationships or hierarchical relations among the features. We can reduce feature's number analyzing relation among the features using heuristic knowledge or statistical method. Heuristic technique refers to learning through repetitive trial and errors and experience. Experts can approach to relevant problem domain through opinion collection process by experience. These properties can be utilized to reduce the number of feature used in learning. Experts generate a new feature (highly abstract) using raw data. This paper describes machine learning model that reduce the number of features used in learning using heuristic function and use abstracted feature by neural network's input value. We have applied this model to the win/lose prediction in pro-baseball games. The result shows the model mixing two techniques not only reduces the complexity of the neural network model but also significantly improves the classification accuracy than when neural network and heuristic model are used separately.

• Journal of Embryo Transfer
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• v.11 no.2
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• pp.111-124
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• 1996

The present study was carried out to investigate the effects of co-culture cells and growth factors on in vitro culture of Korean native cattle(KNC) embryos fertilized in vitro. Two-eight cell embryos were cultured in vitro using 4 types of co-culture cells and 3 growth factors singly or in combination. The results were as follows, In the co-culture of 2~8 cell embryos with bovine oviductal epithelial cell(BOEC), granulosa cell(BGC), uterine epithelial cell(BUEC) and mouse embryonic fibroblast (MEF) monolayers, the developing rate to blastocysts was significantly(P<0.05) higher with BUEC(32.1%) than with MEF(15.3%), BGC(13.2%) and non co-culture control(11.6%). When the morula co-cultured with BOEC for 5 days following in vitro fertilization were co-cultured with BOEC continuously or with BUEC, respectively, the developing rate to blastocysts was higher with BUEC(73.9%) than with BOEC(56.0%). To examine the effects of growth factors on in vitro development of 2~8 cell embryos, epidermal growth factor(EGF), transforming growth factor-$\beta$l(TGF-$\beta$l) and insulin-like growth factor-1(IGF-1) were added singly or in combination to TCM 199 maturation medium with respective concentration. In a addition of each 10, 30 and SOng /rnl EGF, the developing rate to blastocysts was the highest in lOng /ml EGF(25.3%). In addition of each 1, 2 and Sng /mi TGF-$\beta$1, the developing rate to blastocysts was the highest in lng /ml TGF-$\beta$1(28.8%). In addition of each 50, 100ng/ml JGF-l, the developing rate to blastocysts was higher in 100ng/ml IGF-l(16.5%) than in SOng/mi IGF-1(12.9%). When lOng /ml EGF and lng /ml TGF-$\beta$l was added singly or in combination, the developing rate to blastocysts was similar in groups added singly or in combination with EGF and TGF-$\beta$l (23.l~24.6%), although higher than in control(16.7%). In the co-culture of 2~8 cell embryos Wth BOEC + each 10, 30 and 5Ong /rnl EGF, the developing rate to blastocysts was significantly(p<0.05) higher in BOEC + long /ml EGF(32.3%) than in BOEC + 3Ong /ml EGF(18.9%) and BOEC + song /ml EGF(9.7%). In the co-culture of 2~8 cell embryos with BOEC + each 1, 2, Sng /ml TGF-$\beta$l the developing rate to blastocysts was higher in BOEC + Sng/rnl TGF-$\beta$l(28.2%) than in BOEC + lng /ml TGF-$\beta$l(21.7%) and BOEC + 2ng/ml TGF-$\beta$l(21.4%). In summary, higher developing rate to blastocysts were obtained with co-culture of BUEC for co-culture system, with addition of lOng /ml EGF or lng /ml TGF-$\beta$l for growth factor culture system, and with co-culture of BOEC + lOng /ml EGF or BOEC + Sng /ml TGF-$\beta$l for co-culture + growth factor culture system.

• Microbiology and Biotechnology Letters
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• v.42 no.2
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• pp.170-176
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• 2014

In this study, the anti-oxidative and anti-inflammatory activities of Euptelea pleiosperma ethanol extract (EPEE) were evaluated using in vitro assays and cell culture model systems. EPEE possessed a more potent scavenging activity against 1,1-diphenyl-2-picryl hydrazyl than the ascorbic acid used as a positive control. EPEE effectively suppressed lipopolysaccharide (LPS), in addition to hydrogen peroxide induced reactive oxygen species on RAW 264.7 cells. Furthermore, EPEE induced the expression of the anti-oxidative enzyme heme oxygenase 1 (HO-1) and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2), dose and time dependently. The modulation of HO-1 and Nrf2 expression might be regulated by mitogen-activated protein kinases and phosphatidyl inositol 3 kinase/Akt as their upstream signaling pathways. On the other hand, EPEE inhibited LPS induced nitric oxide (NO) formation without cytotoxicity. Suppression of NO formation was the result of the down regulation of inducible NO synthase (iNOS) by EPEE. Suppression of NO and iNOS by EPEE may be modulated by their upstream transcription factor, nuclear factor ${\kappa}B$, and AP-1 pathways. Taken together, these results provide important new insights into E. pleiosperma, namely that it possesses anti-oxidative and anti-inflammatory activities, indicating that it could be utilized as a promising material in the field of nutraceuticals.

• Korean Journal of Environmental Agriculture
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• v.27 no.1
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• pp.60-65
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• 2008

Current soil remediation principles for toxic metals have some limitations even though they vary with different technologies. An alternative technology that transforms hazardous substances into nonhazardous ones would be environmentally beneficial. Objective of this research was to assess optimum conditions for Cr(VI) reduction in soils as influenced by ZVI(Zero-Valent Iron), organic matter and moisture content. The reduction ratio of Cr(VI) was increased from 37 to 40% as organic matter content increased from 1.07 to 1.75%. In addition, Cr(VI) concentration was reduced as soil moisture content increased, but the direct effect of soil moisture content on Cr(VI) reduction was less than 5% of the Cr(VI) reduction ratio. However, combined treatment of ZVI(5%), organic matter(1.75%) and soil moisture(30%) effectively reduced the initial Cr(VI) to over 95% within 5 days and nearly 100% after 30 days by increasing oxidation of ZVI and concurrent reduction of Cr(VI) to Cr(III). The overall results demonstrated that ZVI was effective in remediating Cr(VI) contaminated soils, and the efficiency was synergistic with the combined treatments of soil moisture and organic matter.

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.143-151
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• 2002

The present study was conducted to investigate the effects of antioxidants and growth factors on early development of porcine follicular oocytes fertilized in vitro. The embryos without cumulus cells were transferred into NCSU 23 medium and cultured for 5~6 days. The proportions of embryos developed to morula and blastocyst stages were significantly higher (P＜0.05) in medium with 1.0(21.1%) and 2.0mM(22.3%) NAC than in control (14.7%) and 0.5mM NAC (12.2%). When the embryos were cultured in medium with ebselen, the highest proportion(36.9%) of embryos developed to morula or blastocyst stages was obtained in medium with 10 $\mu$M ebselen. Higher proportions of embryos were developed to rnorula and blastocyst stages during culture with 100$\mu$M than control, 50 and 200$\mu$M glutathione. The EGF and PDGF were evaluated as growth factors supplemented to the culture medium for in vitro development of porcine embryos. The proportions of embryos developed to morular and blastocyst stages were significantly higher (P＜0.05) in medium with 100ng/m1 ECF than control, 10 and 50ng/$m\ell$ EGF. However, the proportions of embryos developed to morular and blastocyst stages were not significantly different among concentrations of PDGF. In conclusions, these results indicate that NAC, ebselen and glutathione or EGF and PDGF can increase the proportion of embryos that develop beyond morula stage.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.301-308
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• 2008

DNA variation of MYL2 intron 5 A345G and ADCYAP1R1 intron 2 A337G were investigated for carcass trait association in finished pigs. Three genotypes(two homozygotes and their heterozygote) were found at 10.6% AA, 45.6% AG and 43.8% GG in MYL2 and 60.5% AA, 34.6% AG, and 22.2% GG for ADCYAP1R1. In finished pig population, individuals containing genotype G- of MYL2 had significantly heavier carcass weight by more than 2.4 kg and thicker backfat thickness by more than 1.3 mm than those of AA homozygous pigs(p<0.05). No significant difference was found in other traits tested in this study such as marbling score, meat color, texture, moisture and separation score(p>0.05). The ADCYAP1R1 intron 2 377GG homozygotes showed coarse texture, i.e., meat quality was inferior than those of AG and AA genotypes, and the moisture level of homozygote AA was higher than those of AG and GG genotypes(p<0.05). The other carcass traits were not significantly associated with ADCYAP1R1 genotypes(p>0.05). The genetic polymorphism of MYL2 and ADCYAP1R1 genes affected the carcass traits in finished pig population. Further studies to explain the association between genetic variations and their phenotypic effects including economic traits in pigs are required including critical mutation in both genes through molecular approaches.

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.347-353
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• 2005

Pesticide risk assessment for pesticide operators as well as for consumers has become one of the pesticide regulatory tools to reduce any unreasonable adverse health effects from pesticide use. The risk for pesticide operators can be quantified by comparing the acceptable operator exposure level(AOEL) with exposure level during pesticide application. This study is to evaluate the risk of benzimidazole fungicides application worker. The exposure level of pesticide applicators were calculated using Japanese operator exposure study tested with EPN 45% EC. The AOELs for pesticides were obtained dividing relevant lowest no observed abuse effect levels(NOAELs) for the exposure scenario into uncertainty factor, 100. For the non-cancer and cancer occupational risk assessment, $Q_1^*$ produced by US/EPA and life time average daily dose(LADD) calculated from average daily dose(ADD), treatment days per year, worked years for life time were used. Operator exposure for benzimidazole fungicides application were benomyl 0.2, carbendazim 0.36 and thiophanate-methyl 0.42 mg/kg/day. Short-term AOELs for benomyl, carbendazim and thiophanate-methyl were 0.3, 0.1, and 0.2 mg/kg/day, and long-term AOEL were 0.025, 0.025, 0.08 mg/kg/day, respectively. LADDs were benomyl 0.0038, carbendazim 0.0067, thiophanate-methyl 0.0081 mg/kg/day. The ratios of exposure to AOEL were $0.28{\sim}1.5$ for short-term and $3.73{\sim}9.88$ for long-term. Cancer risk for operator were $9.12{\times}10^{-6}$ for benomyl, $1.61{\times}10^{-5}$ for carbendazim and $1.13{\times}10^{-4}$ for thiophanate-methyl by the standard application scenario. The result showed 3 fungicides exceed the risk criteria, $1.0{\times}10^{-6}$. The above risk assessments were based upon conservative assumptions and therefore are believed to be protective of the applicator. To refine the risk at the more actual conditions, further risk assessment with more realistic data would be needed.

• Journal of the Korean Institute of Landscape Architecture
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• v.44 no.4
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• pp.86-99
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• 2016

This study was for analyzing the external environment and internal space structure and improving the way of use efficiency in waterfront through the Dragon boat festival to utilize waterfront actively. Through from the four target area, Hongkong, Busan, Incheon and Daejeon, this study was for an importance and satisfaction analysis for users about the element effect on the waterfront use efficiency and the contribution to cultural contents revitalization of waterfront by giving basic data. The result is as follows. First, in the importance analysis about 12 items, modern cultural infra around the waterfront was ranked highest, 8.26 and waterfront landscape, square & openspaces, convenience facilities, transport, green area, quality of viewing space, historic resources, pedestrian, suitability of width, wave, depth, water quality, berth & mooring were ranked in descending order. Second, waterfront landscape was interpreted by rather the external environmental impact according to city size than the matter of spatial structure in target area and judged as an important factor effect on site selection for waterfront. In the analysis of waterfront landscape, the reason of the high satisfaction about domestic target area was that riverside parks were recently made considering their waterfront activities. Viewing space was major infra where people could experience the pleasant waterfront and watch dynamic water leisure sports like Dragon boat three dimensionally and was thought to be improved for the use efficiency. Third, tourism resources were very important element that affect the use efficiency of waterfront, so waterfront users react sensitively to modern tourism resources rather than to historic resources. This meant that tourism infrastructure for shopping and leisure of the young affected the use efficiency of waterfront, so Hongkong and Busan were in a better position in terms of using waterfront that was near the tourism infrastructure. Fourth, in the analysis of traffic accessibility, both Hongkong and Busan were high evaluated in terms of excellent traffic accessibility by subway. Daejeon was low rated in terms of the satisfaction of use efficiency, because of the relative lower place awareness compared with transportation infrastructure. In Hongkong, waterfront was connected with downtown and in Busan, housing complex and shopping centers were located in the place for users in an easily accessible on foot, so the satisfaction was high-pitched. Finally, in the importance of water surface width and the analysis of satisfaction, except Incheon, all the three were over 200m in width of water surface and this meant the surface width above certain level was interpreted to interrupt the concentration of enjoying the water leisure sports. In the analysis of surface condition such as water quality, water depth and wave, through a survey, Busan had a problem with water quality and Gapcheon in Daejeon had a problem with optimal water depth by the festival participants.

• Korean Journal of Remote Sensing
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• v.31 no.6
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• pp.549-560
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• 2015

With the global scale impact of atmospheric $CO_2$ in global warming and climate system, it is necessary to monitor the $CO_2$ concentration continuously on a global scale, where satellite remote sensing has played a significant role recently. In this study, global monthly $CO_2$ concentrations obtained by satellite remote sensing were compared with ground-based measurements at Anmyeon-do and Gosan Korean Global Atmosphere Watch Center. Atmospheric $CO_2$ concentration has increased from 371.87 ppm in January 1999 to 405.50 ppm in December 2013 at Anmyeon-do station (KMA, 2013). Comparison of the continuous measurements by flask air sampling at Anmyeon-do shows the same trend and seasonal variations with those of global monthly mean dataset. Nevertheless, the trends of $CO_2$ over Northeast Asia showed the higher than those of global and the trends also changes with different slope. $CO_2$ products derived from Greenhouse Gases Observing Satellite (GOSAT) and Atmospheric Infrared Sounder (AIRS) were compared with ground-based measurement at Anmyeon-do. The monthly mean values of GOSAT and AIRS data are systemically lower than those obtained at Anmyeon-do, however, the seasonal cycle of satellite products present the similar trend with values of global and Anmyeon-do. The accuracy of $CO_2$ products from GOSAT and AIRS were evaluated statistically for two years from January 2011 to December 2012. GOSAT showed good correlation with the correlation coefficient, RMSD and bias of 0.947, 5.610 and -5.280 to ground-based measurements respectively, while AIRS showed reasonable comparison with 0.737, 8.574 and -7.316 at Anmyeon-do station, respectively.