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http://dx.doi.org/10.4014/kjmb.1403.03002

Anti-Oxidative and Anti-Inflammatory Activities of Euptelea Pleiosperma Ethanol Extract  

Jin, Kyong-Suk (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Park, Jung Ae (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Lee, Ji Young (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Kang, Ji Sook (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, College of Natural Science, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.2, 2014 , pp. 170-176 More about this Journal
Abstract
In this study, the anti-oxidative and anti-inflammatory activities of Euptelea pleiosperma ethanol extract (EPEE) were evaluated using in vitro assays and cell culture model systems. EPEE possessed a more potent scavenging activity against 1,1-diphenyl-2-picryl hydrazyl than the ascorbic acid used as a positive control. EPEE effectively suppressed lipopolysaccharide (LPS), in addition to hydrogen peroxide induced reactive oxygen species on RAW 264.7 cells. Furthermore, EPEE induced the expression of the anti-oxidative enzyme heme oxygenase 1 (HO-1) and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2), dose and time dependently. The modulation of HO-1 and Nrf2 expression might be regulated by mitogen-activated protein kinases and phosphatidyl inositol 3 kinase/Akt as their upstream signaling pathways. On the other hand, EPEE inhibited LPS induced nitric oxide (NO) formation without cytotoxicity. Suppression of NO formation was the result of the down regulation of inducible NO synthase (iNOS) by EPEE. Suppression of NO and iNOS by EPEE may be modulated by their upstream transcription factor, nuclear factor ${\kappa}B$, and AP-1 pathways. Taken together, these results provide important new insights into E. pleiosperma, namely that it possesses anti-oxidative and anti-inflammatory activities, indicating that it could be utilized as a promising material in the field of nutraceuticals.
Keywords
Euptelea pleiosperma; anti-oxidative activity; anti-inflammatory activity; upstream transcription factor;
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1 Bryan HK, Olayanju A, Goldring CE, Park BK. 2013. The Nrf2 cell defence pathway: Keap1-dependent and -independent mechanisms of regulation. Biochem. Pharmacol. 85: 705-717.   DOI   ScienceOn
2 Cencioni C, Spallotta F, Martelli F, Valente S, Mai A, Zeiher AM, et al. 2013. Oxidative stress and epigenetic regulation in ageing and age-related diseases. Int. J. Mol. Sci. 14: 17643- 17663.   DOI
3 Chapple SJ, Siow RC, Mann GE. 2012. Crosstalk between Nrf2 and the proteasome: therapeutic potential of Nrf2 inducers in vascular disease and aging. Int. J. Biochem. Cell Biol. 44: 1315-1320.   DOI   ScienceOn
4 Giudice A, Arra C, Turco MC. 2010. Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents. Meth. Mol. Biol. 647: 37-74.   DOI   ScienceOn
5 Gonzalez-Burgos E, Gomez-Serranillos MP. 2012. Terpene compounds in nature: a review of their potential antioxidant activity. Curr. Med. Chem. 19: 5319-5341.   DOI
6 Grivennikov SI, Greten FR, Karin M. 2010. Immunity, inflammation, and cancer. Cell. 140: 883-899.   DOI   ScienceOn
7 Hu R, Saw CL, Yu R, Kong AN. 2010. Regulation of NF-E2- related factor 2 signaling for cancer chemoprevention: antioxidant coupled with antiinflammatory. Antioxid. Redox. Signal. 13: 1679-1698.   DOI   ScienceOn
8 Kalyanaraman B. 2013. Teaching the basics of redox biology to medical and graduate students: Oxidants, antioxidants and disease mechanisms. Redox. Biol. 1: 244-257.   DOI   ScienceOn
9 Kedare SB, Singh RP. 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48: 412-422.   DOI   ScienceOn
10 Kundu JK, Surh YJ. 2012. Emerging avenues linking inflammation and cancer. Free Radic. Biol. Med. 52: 2013-2037.   DOI   ScienceOn
11 Zhang M, An C, Gao Y, Leak RK, Chen J, Zhang F. 2013. Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection. Prog. Neurobiol. 100: 30-47.   DOI   ScienceOn
12 Zhang R, Kang KA, Piao MJ, Maeng YH, Lee KH, Chang WY, et al. 2009. Cellular protection of morin against the oxidative stress induced by hydrogen peroxide. Chem. Biol. Interact. 177: 21-27.   DOI   ScienceOn
13 Zhao CR, Gao ZH, Qu XJ. 2010. Nrf2-ARE signaling pathway and natural products for cancer chemoprevention. Cancer Epidemiol. 34: 523-533.   DOI   ScienceOn
14 Awad N, Khatib N, Ginsberg Y, Weiner Z, Maravi N, Thaler I, et al. 2011. N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation. Am. J. Obstet. Gynecol. 450: e15-20.
15 Chawla A, Nguyen KD, Goh YP. 2011. Macrophage-mediated inflammation in metabolic disease. Nat. Rev. Immunol. 11: 738-749.   DOI   ScienceOn
16 Lee JC, Hou MF, Huang HW, Chang FR, Yeh CC, Tang JY, et al. 2013. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell Int. 13: 55.   DOI   ScienceOn
17 Khansari N, Shakiba Y, Mahmoudi M. 2009. Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent Pat. Inflamm. Allergy Drug Discov. 3: 73-80.   DOI   ScienceOn
18 Kocanova S, Buytaert E, Matroule JY, Piette J, Golab J, de Witte P, et al. 2007. Induction of heme-oxygenase 1 requires the p38MAPK and PI3K pathways and suppresses apoptotic cell death following hypericin-mediated photodynamic therapy. Apoptosis. 12: 731-741.   DOI   ScienceOn
19 Kundu JK, Surh YJ. 2008. Inflammation: gearing the journey to cancer. Mutat. Res. 659: 15-30.   DOI   ScienceOn
20 Li J, Zhang H, Huang W, Qian H, Li Y. 2012. TNF-$\alpha$inhibitors with anti-oxidative stress activity from natural products. Curr. Top Med. Chem. 12: 1408-1421.   DOI
21 Li L, Dong H, Song E, Xu X, Liu L, Song Y. 2014. Nrf2/ARE pathway activation, HO-1 and NQO1 induction by polychlorinated biphenyl quinone is associated with reactive oxygen species and PI3K/AKT signaling. Chem. Biol. Interact. 209: 56-67.   DOI
22 Liochev SI. 2013. Reactive oxygen species and the free radical theory of aging. Free Radic. Biol. Med. 60: 1-4.   DOI   ScienceOn
23 Lu Y, Suh SJ, Kwak CH, Kwon KM, Seo CS, Li Y, et al. 2012. Saucerneol F, a new lignan, inhibits iNOS expression via MAPKs, NF-$\kappa{B}$ and AP-1 inactivation in LPS-induced RAW264.7 cells. Int. Immunopharmacol. 12: 175-181.   DOI
24 Noworyta-Sokolowska K, Gorska A, Golembiowska K. 2013. LPS-induced oxidative stress and inflammatory reaction in the rat striatum. Pharmacol. Rep. 65: 863-869.   DOI
25 Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic. Biol. Med. 49: 1603-1616.
26 Saw CL, Wu Q, Su ZY, Wang H, Yang Y, Xu X, et al. 2013. Effects of natural phytochemicals in Angelica sinensis (Danggui) on Nrf2-mediated gene expression of phase II drug metabolizing enzymes and anti-inflammation. Biopharm. Drug Dispos. 34: 303-311.   DOI   ScienceOn
27 Park CM, Jin KS, Lee YW, Song YS. 2011. Luteolin and chicoric acid synergistically inhibited inflammatory responses via inactivation of PI3K-Akt pathway and impairment of NF-$\kappa{B}$ translocation in LPS stimulated RAW 264.7 cells. Eur. J. Pharmacol. 660: 454-459.   DOI   ScienceOn
28 Park CM, Park JY, Noh KH, Shin JH, Song YS. 2011. Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-$\kappa{B}$ modulation in RAW 264.7 cells. J. Ethnopharmacol. 133: 834-842.   DOI   ScienceOn
29 Pillai S, Oresajo C, Hayward J. 2005. Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation- induced matrix degradation - a review. Int. J. Cosmet. Sci. 27: 17-34.   DOI   ScienceOn
30 Su ZY, Shu L, Khor TO, Lee JH, Fuentes F, Kong AN. 2013. A perspective on dietary phytochemicals and cancer chemoprevention: oxidative stress, nrf2, and epigenomics. Top Curr. Chem. 329: 133-162.
31 Tsai HH, Lee WR, Wang PH, Cheng KT, Chen YC, Shen SC. 2013. Propionibacterium acnes-induced iNOS and COX-2 protein expression via ROS-dependent NF-$\kappa{B}$ and AP-1 activation in macrophages. J. Dermatol. Sci. 69: 122-131.   DOI   ScienceOn
32 Wang FW, Wang Z, Zhang YM, Du ZX, Zhang XL, Liu Q, et al. 2013. Protective effect of melatonin on bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis in vitro. J. Cell Biochem. 114: 2346-2355.   DOI   ScienceOn
33 Yagi H, Tan J, Tuan RS. 2013. Polyphenols suppress hydrogen peroxide-induced oxidative stress in human bone-marrow derived mesenchymal stem cells. J. Cell Biochem. 114: 1163- 1173.   DOI   ScienceOn