• Journal of Life Science
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• v.22 no.4
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• pp.456-465
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• 2012

Heterodimeric recombinant human bone morphogenetic proteins (rhBMPs) are powerful tools for bone tissue engineering. However, BMPs have several important limitations in their application to bone regeneration. BMPs have a short half-life and must be used in high concentrations, which may be cost-inefficient. To overcome these problems, we established a stable cell line that expressed the fusion protein comprised of recombinant human BMP2/7 heterodimer protein and PTD (rhBMP2/7-PTD). This stable cell line enabled high process yields by continuously expressing rhBMP2/7-PTD products at high levels throughout cultivation. This synthesized BMP7 was fused to a BMP2 protein with four glycine residues (to allow free bond rotation of the domains) and PTD. To demonstrate that the rhBMP2/7-PTD protein that was secreted from an rhBMP2/7-PTD-expressing stable cell line exhibited biological activity consistent with its role as an osteogenic differentiation induction growth factor, we evaluated BMP-induced ALP activity. Our results suggest that this cell line may be a powerful and efficient tool for applications such as bone tissue regeneration.

• Journal of the Korean Applied Science and Technology
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• v.38 no.4
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• pp.1003-1009
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• 2021

A study on the associative properties of sodium hyaluronate (NaHA) and Alkane-bis (dimethylalkylammonium bromide) surfactants in aqueous solution was investigated in relation to the chemical structure of surfactants. As a result of measuring the interfacial tension, a parabolic graph showing the minimum value (c_min) at a specific concentration was shown. Above this minimum concentration the increase in interfacial tension is thought to be related to the formation of aggregates of NaHA chains and dimeric surfactants. The plot of viscosity vs surfactant concentration shows a slight maxium at c_min and a viscosity decrease at high surfactant concentrations. Viscosity nonlinear behavior is related to the size increase due to the complex growth and to the size shrinkage following from the interaction with electrolyte ions and free micelles. The results of surface tension measurements show a broad region of surface tension decrease, indicating the NaHA-surfactant interaction. The increase in surface tension above c_min may be related to the adsorption of clusters, consisting of free NaHA chains and dimeric surfactant. The strong adsorption of surfactant is observed at high concentrations.

• Journal of the Korean Chemical Society
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• v.20 no.1
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• pp.56-58
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• 1976

A new mechanism is proposed for the reduction of nitrobenzene in basic solution that does not involve hydroxylamine as an intermediate. This paper presents evidence that the azoxybenzene is not formed from the hydroxylamine, but formed instead from the dimerization of nitrosobenzene.

• Journal of the Korean Chemical Society
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• v.44 no.2
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• pp.166-169
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• 2000

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.6
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• pp.1566-1571
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• 2007

Oligodeoxyribonucleotide bearing the di-acetylenic linkage has been prepared. Staring from 5-Iodo-2'-deoxyuridine(1), a four-step sequence, consisting of the Pd(0)-catalyzed Heck-type C-C coupling with acetylenic group, protection of 5'-hydroxy group, generation of acetylenic hydrogen, and Glaser oxidative coupling reaction leads to the dimeric oligodeoxyribonucleotide(5).

• Journal of the Korean Chemical Society
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• v.55 no.3
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• pp.392-399
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• 2011

Two conformations of benzoic acid derivatives ($NH_2$, OH, H, F, Cl, CN, NO, $NO_2$) have been investigated at MP2, DFT and HF level using the 6-311++G(d,p) basis set. It was found that the cis isomers are more stable. Hydrogen bonding formation of benzoic acids has been estimated from stabilization energies. The calculated hydrogen-bonding energies of dimers showed a cooperative interaction in the cyclic ones. It was found that an electron-releasing group (ERG) into the phenyl rings resulted in the formation of more stable hydrogen bonding. Red shift of O-H bond was found from -565.3 to -589.3 for dimers. The natural bond orbital (NBO) analysis was applied to characterize nature of the interaction.

• Korean Journal of Food Science and Technology
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• v.41 no.5
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• pp.477-482
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• 2009

Toll-like receptors (TLRs) play a critical role in the induction of innate immune responses that are essential for host defense against invading microbial pathogens. In general, TLRs have two major downstream signaling pathways, namely MyD88- and TRIF-dependent pathways, leading to the activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and interferon regulatory factor 3 (IRF3) and the expression of inflammatory mediators. TLR4 dimerization is required for the activation of downstream signaling pathways and may be one of the first lines of regulation in activating TLR-mediated signaling pathways. In this paper, the molecular targets of curcumin, 6-shogaol, and cinnamaldehyde in TLR signaling pathways will be discussed. Curcumin, 6-shogaol, and cinnamaldehyde with ${\alpha},{\beta}$-unsaturated carbonyl groups inhibit the dimerization of TLR4 induced by lipopolysaccharide, resulting in the downregulation of NF-${\kappa}B$ and IRF3. These results suggest that phytochemicals with the structural motif conferring Michael addition inhibit TLR4 dimerization, suggesting a novel mechanism for the anti-inflammatory activity of phytochemicals.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.8-15
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• 2004

Hwangjenegyeng somun. Treatise on the parting and Meeting of Yin-Yang is the theory of the parting and meeting about Yin-Yang. It is harmonized treatise Yin-Yang and systemic constant. Taechung and Gwangmyeng are the concretely applied form of the parting and meeting of Yin-Yang at human body. The parting and Meeting of Three-Yin- Three-Yang has six types symbols of circulated Yin-Yang. They have Six-meeting space structure as match like up-down, backside and front-side, left-side and right-side. These pairs express symbolized meaning of creation of Saeng-jang-shu-chang and also apply the same things. Somum Treatise on the Parting and Meeting of Yin-Yang explains acting of human body by the parting and meeting of Three-yin-three-yang as six structures of Opening-covering-axis of Three-yin-three-yang. This principal is expression of the parting and meeting of Yin-Yang and concretes the opening and closing of circulation process for symmetry by each acting of Three-yin-three-yang. As result. the match of six-meeting in space structure and the Opening-covering-axis of Three-yin-three-yang are the basic principal of oriental medicine about the treatise of Gyeng-Lak and the treatise of Six-Gyeng of Sanghanlon.

• Journal of the Korean Chemical Society
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• v.55 no.2
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• pp.199-203
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• 2011

The title one-dimensional hydrogen-bonded coordination compound $[Cu^{II}(C_{13}H_{17}N_3OBr)(C_8H_5O_4)]{\cdot}2H_2O.CH_3OH$ has been synthesized and characterized by single crystal X-ray diffraction study. The monomeric unit contains a square-planar $Cu^{II}$ centre. The four coordination sites are occupied by a tridentate anionic Schiff base ligand (4-bromo-2-[(2-piperazin-1-yl-ethylimino)-methyl]-phenol) which furnishes an $N_2O$-donor set, with the fourth position being occupied by the oxygen atom of an adjacent terephthalate unit. Two adjacent neutral molecules are linked through intermolecular N-H---O and O-H---N hydrogen bonds and generate a dimeric pair. Each dimeric pair is connected with each other via discrete water and methanol molecules by hydrogen bonding to form a one-dimensional supramolecular network.

• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.813-820
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• 2019

The influence of cholesterol on the physicochemical properties of the oleic acid was clarified through the measurements of density, viscosity, IR, $^1H$ NMR, self-diffusion coefficient for the oleic acid samples containing a small amount of additives such as cholesterol, cholestanol, cholestane, cholesteryl oleate, benzene, and ethanol. Cholesterol, possessing one OH group and one double bond in its molecular structure, largely increased the viscosity and reduced the self-diffusion coefficient and the intramolecular movement of oleic acid. Oleic acid forms a complex with cholesterol as well as with ethanol. On the basis of these complex formations and the existence of the clusters composed of oleic acid dimers, it was known the role and the fundamental mechanism of cholesterol to the intermolercular and intramolecular movements of oleic acid in the liquid state.