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http://dx.doi.org/10.5352/JLS.2012.22.4.456

Establishment of a Stable Cell Line Expressing Human BMP2/7-PTD for Efficient Osteogenic Induction  

Park, Seung-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kang, Seok-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Goo, Tae-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Paik, Soon-Young (Department of Microbiology, College of Medicine, The Catholic University of Korea)
Publication Information
Journal of Life Science / v.22, no.4, 2012 , pp. 456-465 More about this Journal
Abstract
Heterodimeric recombinant human bone morphogenetic proteins (rhBMPs) are powerful tools for bone tissue engineering. However, BMPs have several important limitations in their application to bone regeneration. BMPs have a short half-life and must be used in high concentrations, which may be cost-inefficient. To overcome these problems, we established a stable cell line that expressed the fusion protein comprised of recombinant human BMP2/7 heterodimer protein and PTD (rhBMP2/7-PTD). This stable cell line enabled high process yields by continuously expressing rhBMP2/7-PTD products at high levels throughout cultivation. This synthesized BMP7 was fused to a BMP2 protein with four glycine residues (to allow free bond rotation of the domains) and PTD. To demonstrate that the rhBMP2/7-PTD protein that was secreted from an rhBMP2/7-PTD-expressing stable cell line exhibited biological activity consistent with its role as an osteogenic differentiation induction growth factor, we evaluated BMP-induced ALP activity. Our results suggest that this cell line may be a powerful and efficient tool for applications such as bone tissue regeneration.
Keywords
Bone morphogenetic protein; protein transduction domain; stable cells; bone tissue regeneration;
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