• Journal of the Korean Society of Food Culture
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• v.21 no.3
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• pp.319-324
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• 2006

Volatile compounds in low-temperature and long-term fermented Baechu kimchi were extracted by high vacuum sublimation(HVS), and then analyzed by gas chromatography/mass spectrometry(GC-MS). A total of 62 compounds, including 7 sulfur-containing compounds, 8 terpenes, 5 esters, 8 acids, 15 alcohols, 2 nitrites, 2 ketones, 11 aliphatic hydrocarbons and 4 miscellaneous compounds, were found in low-temperature and long-term fermented Baechu kimchi. Among them, acetic acid and butanoic acid were quantitatively dominant. Aroma-active compounds were also determined by gas chromatography/olfactometry(GC-O) using aroma extract dilution analysis(AEDA). A total of 16 aroma-active compounds were detected by GC-O. Butanoic acid was the most potent aroma-active compound with the highest FD factor($Log_3FD$) followed by linalool, acetic acid, 2-vinyl-4H-1,3-dithin and 3-methyl-1-butanol. The major aroma-active compounds, such as acetic acid and butanoic acid, were related to sour and rancid or notes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1654-1661
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• 2011

We investigated the effect of Angelica keiskei ethanol (AKE) extract on cell death in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the presence 125, 150 and 175 ${\mu}g$/mL concentrations of AKE for 24 hours. MTT assays demonstrated that mitochondrial dehydrogenase activities decreased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In contrast, the proportion of dual staining with Hoechst 33342/ethidium bromide(EtBr) for cell death increased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In particular, the levels of cell death caused by apoptotic program showed marked increases in the 150 and 175 ${\mu}g$/mL AKE groups, as revealed by flow cytometry. An apoptotic suppressor gene, Bcl-2, significantly decreased at the transcript level (p<0.05). The expression levels of proapoptotic genes, both Bax and caspase 3 significantly increased (p<0.05). Furthermore, the ratio of Bcl-2/Bax mRNA which is considered to be an important indicator of apoptosis, significantly decreased in a dose-dependent manner (p<0.05). These results taken together indicate that, the AKE extract used in this study induces cell death in MDA-MB-231 human breast cancer cells.

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.1812-1816
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• 2009

이미지 해석에 의한 유속장 측정방법은 유체역학분야에서 지난 30 여년 동안 많이 활용되어온 속도측정 기법으로 오늘날에는 이를 수공학 분야에서 이를 유량측정 등 수리현상 해석에 활용하려는 시도가 다각적으로 이루어지고 있다. 이에 본 연구에서는 이미지 해석에 의한 유속장 측정방법을 용담댐 시험유역에 적용하여 그의 자연하천에서의 적용성을 검토하고자 한다. 이미지 해석에 의한 유속장 측정방법은 PIV(Particle Image Velocimetry)로 통칭되고 있으며, PIV는 seeding, illumination, recording, 및 image processing의 네 가지 요소로 구성된다. seeding을 위해서 유체를 따라 흐를수 있는 작은 입자를 유체에 첨가한다. 유체를 따라 흐르는 입자들의 선명한 이미지를 얻기 위해서illumination이 필요하다. PIV를 이용하여 흐름을 해석하기 위한 illumination은 일반적으로 이중펄스 레이저가 이용된다. 이렇게 유속장 해석을 하려는 유체에 대하여 seeding 및 illumination이 준비되면 단일노출- 다중 프레임법, 혹은 다중노출-단일 프레임법으로 흐름을 recording을 한다. image processing은 이미지를 다운로드하고, 디지타이징 및 화질향상을 하는 전처리(pre-processing), 상관계수의 산정에 의한 유속 벡터의 결정 및 에러 벡터를 제거하고 유속장을 그래프화하는 후처리(post-processing) 과정으로 구성된다. LSPIV(Large Scale PIV)는 PIV의 기본원리를 근거로 하여 기존의 PIV에 비하여 실험실 내에서의 수리모형실험이나 일반 하천에서의 유속측정과 같은 큰 규모$(4m^2\sim45,000m^2$)의 흐름해석을 할 수 있도록 Fujita et al.(1994)와 Aya et al.(1995)이 확장시킨 것이다. PIV와 비교시 LSPIV의 다른 점은 넓은 흐름 표면적을 포함하기 위하여 촬영시에 카메라의 광축과 흐름 사이의 각도가 PIV에서 이용하는 수직이 아닌 경사각을 이용하였고 이에 따라 발생하는 이미지의 왜곡을 제거하기 위하여 이미지 변환기법을 적용하여 왜곡이 없는 정사촬영 이미지로 변환시킨다. 이후부터는 PIV의 이미지 처리 방법이 적용되어 표면유속을 산정한다. 다만 이미지 변환을 PIV 이미지 처리 전에 하느냐 후에 하느냐에 따라 유속장 해석결과에 차이가 있다. PIV의 네가지 단계를 포함하여 LSPIV의 각 단계를 구분하면, seeding, illumination, recording, image transformation,image processing 및 post-processing의 여섯 단계로 나뉘어진다 (Li, 2002). LSPIV를 적용시 물표면 입자의 Tracing을 위하여 자연하천에서 사용하기에 적합한 환경친화적인 seeding 재료인 Wood Mulch를 사용하여 유속을 측정하였다. 적용지점은 용담댐 상류의 동향수위관측소 지점으로 이 지점은 한국수자원공사의 수자원시험유역이 위치하고 있다. 이미지의 촬영은 가정용 비디오 캠코더 (Sony DCR-PC 350)을 이용하여 두 줄기의 흐름에 대하여 각각 약 5분 동안의 영상을 촬영한후 이중에서 seeding의 분포가 잘 이루어진 약 1분간을 추출한후 이를 이용하여 PIV 분석에 이용하였다. 대체적으로 유속장의 계산이 무난하게 이루어지었으나 비교적 수질 상태가 양호하고, 수심이 낮고, 하상재료가 자갈로 이루어져 있어 비슷한 색상의 seeding 재료를 추적하기 어려운 구간이 발생한 부분에서는 유속의 계산이 정확히 이루어지지 않았다.

• Journal of Energy Engineering
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• v.24 no.3
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• pp.40-47
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• 2015

Integrated gasification combined cycle (IGCC) power plants not only emit less greenhouse gases and air pollutants than conventional coal-fired power plants, but also use low-price, low-quality, and internationally easily procurable coal. Thus we can benefit from safeguarding energy security through building the IGCC power plant. This paper attempts to value the benefits of energy security enhanced by IGCC power plant. To this end, we report here the results from a contingent valuation survey of randomly selected 600 households. A combination of a double-bounded model and a spike model is applied for the purpose of increasing statistical efficiency and dealing with zero(0) willingness to pay data, respectively. The results show that the respondents are additionally willing to pay 6.05 won for 1kWh of electricity generated from IGCC power plant. In other words, the benefits from safeguarding energy security through building the IGCC power plant are 6.05 won per kWh. Given that the expected amount of generation from the Taean IGCC power plant that is scheduled to be built in late 2015 is 2.27 TWh per year, the benefits are estimated to be 13.74 billion won per year.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.6
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• pp.1042-1049
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• 2000

Irradiation-induced volatile flavor compounds in irradiated (1, 3, 5, 10 kGy) beef were analyzed by liquid liquid continuous extraction (LLCE) and gas chromatography/mass spectrometry (GC/MS) methods. One hundred fifty volatile compounds were detected in irradiated beef. These compounds were composed mainly of 71 hydrocarbons, 35 aromatic compounds, 15 aldehydes, 7 ketones, 4 acids, 6 esters and 12 miscellaneous compounds. Among these, only 6 volatile compounds including (E) -2-hexenal, nonene, 2-nonenal, cyclodecene, dodecene and cyclododecene were detected as irradiation-induced volatile flavor compounds, comparing with unirradiated beef meat. However, 4 volatile compounds, such as cyclodecene (r=0.88), (E)-2-hexenal (r=0.85), nonene (r=0.74) and 2-nonenal (r=0.70), having a positive correlation coefficient with the increment of irradiation dose, were considered as marker compounds for detecting irradiation dosages in irradiated beef.

• The Korean Journal of Pesticide Science
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• v.18 no.2
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• pp.79-87
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• 2014

Pyriofenone is an aryl phenyl ketone fungicide that is newly registered in Korea in 2013 to control powdery mildew on food. The objective of this study was to develop reliable and sensitive analytical method for determination of pyriofenone residue in agricultural products for ensuring the food safety. The pyriofenone residues in all samples(Korean melon, pepper, potato, mandarin, soybean, and hulled rice) were extracted with acetonitrile, partitioned with dichloromethane, and then purified with a silica cartridge. The purified samples were analyzed by HPLC-UVD and confirmed with LC-MS. The linear range of pyriofenone was 0.05~5 mg/kg with the correlation coefficient ($r^2$) > 0.999. Average recoveries of pyriofenone ranged from 72.8% to 99.5% at the spiked level of 0.05 and 0.5 mg/kg, while the relative standard deviation was 2.3%~6.4%. In addition, the limit of detection and limit of quantification were 0.01 and 0.05 mg/kg, respectively. The results revealed that the developed and validated analytical method was suitable for pyriofenone determination in agricultural products.

• Food Science and Preservation
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• v.21 no.4
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• pp.593-599
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• 2014

This study was conducted to determine the antioxidant potentials of some available plants to source alternate antioxidants and antibiotics. The antioxidant activity was evaluated by determining the antioxidant activity reducing powers and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities of extracts from some local edible native plants. The DPPH radical scavenging activity of the extracts was found to have been 0.41-94.84%. The Elsholtziasplendens NAKAI extract ($85^{\circ}C$, ethanol, accelerated solvent extraction) showed the highest level of antioxidant activity. Eight samples of plant extracts were evaluated for their antimicrobial activities against three microflora (Bacillus subtilis, Escherichia coli, and Candida albicans) using disc diffusion assay. Two samples (Geranium krameri FR. Et SAV. and Cleyera japonica Thunb.) had much more intensive antimicrobial activities than the control. However, their DPPH free radical scavenging activity levels were only 14.50 and 13.85, respectively. It is suggested that they could be used as natural preservatives against bacterial contamination in cosmetics and foods, in place of the common synthetic preservatives currently used.

• Food Engineering Progress
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• v.21 no.4
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• pp.326-331
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• 2017

A cultivar (Malus domestica cv. Fuji) of apple was selected to make apple peel (AP) powder by three different powdering methods. Frozen AP was thawed and subsequently was dried or ground without drying. After AP was dried by hot-air drying at $60^{\circ}C$ or freeze-drying, the dried AP was ground using a conventional blender. Separately, the thawed AP was powered by using a cryogenic micro grinding technology (CMGT). The ground AP and three types of AP powder were extracted using deionized water, 20, 40, 60, 80, or 100% methanol, followed by vacuum evaporation. The total phenolics contents (TPC), total flavonoids contents (TFC), DPPH, and ABTS radical scavenging capacities of each extract were compared to determine an efficient powdering method. Lyophilized AP powder extract using 60% methanol showed the highest TPC and DPPH radical scavenging capacity. In contrast, 60% methanol extract of the powder by CMGT, resulting in the smallest particle, exhibited the highest TFC and ABTS radical scavenging capacity. This study suggests that the extraction yield of bioactive compounds from AP may be varied according to different powdering methods and that a new powdering process such as CMGT may be applicable to develop functional foods efficiently.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.264-270
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• 2013

Imidacloprid is a systemic insecticide which act as an insect neurotoxin. It used for control of pest such as aphids and other sucking insects in fruits and vegetables. Systemic pesticides move inside a crop following absorption by the plant, and these were converted into a variety of metabolites. Sometimes these metabolites make a problem about safety of agricultural products. So a simultaneous determination method of pesticide and its metabolites is needed, to monitor their presence in agricultural product and study on the fate of pesticide in a plant. This study's aim is to investigate simultaneous analysis method of imidacloprid and its metabolites, imidacloprid guanidine, imidacloprid olefin, imidacloprid urea, and 6-chloronicotinic acid in red pepper using QuEChERS method and LC-MS/MS systems. QuEChERS method was modifed beacuase $MgSO_4$ salts decreased the recoveries of 6-chloronicotinic acid in extraction procedure. Imidacloprid and its metabolites were extracted by acetonitrile with 1% glacial acetic acid and the extracts were purified through QuEChERS with primary secondary amine (PSA) and $C_{18}$ and analyzed with LC-MS/MS in ESI positive mode. Standard calibration curves were made by matrix matched standards and their correlation coefficients were higher than 0.999. Recovery studies were carried out on spiked pepper blank sample at four concentration levels (0.01, 0.04 and 0.1, 0.4 mg/kg). The average recoveries of imidacloprid and its metabolites were in the range of 70~120% with < 20% RSD. This result indicated that the method using QuEChERS and LC-MS/MS was suitable for the simultaneous determination of imidacloprid and its metabolites in red pepper.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.6
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• pp.958-964
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• 2002

To select the irradiation-induced marker components from volatile flavor compounds in irradiated chicken, and complement the extraction problems of liquid continuous extraction (LLCE) method, the volatile compounds of irradiated (0,1,3,5 and 10 kGy) chicken were analyzed by Purge and Trap (P&T) and gas chromatography/mass spectrometry (GC/MS) methods. A total of 119 compounds were detected in irradiated chicken, and these compounds were composed mainly of 7 aldehydes,22 ketones,8 alcohols,30 esters,36 hydrocarbons,8 aromatic compounds and 8 miscellaneous compounds. Among these, only 21 compounds were detected in both LLCE and P&T methods, and the 98 other were detected in omly P&T method. Among volatile compounds detected in irradiated chicken, only 3 compounds such as hexene (r=0.96, p<0.01), propanol (r=0.93, p<0.05) and 1,3-bis(1,1-dimethylethyl) benzene (r=0.96, p<0.05) were newly selected as marker compounds in irradiated chicken by P&T method, which showed significant and high positive correlation coefficient in the change of relative concentration according to the increment of irradiation dosage.