Browse > Article
http://dx.doi.org/10.3746/jkfn.2011.40.12.1654

Effect of Angelica keiskei Extract on Apoptosis of MDA-MB-231 Human Breast Cancer Cells  

Jeong, Yu-Jin (Dept. of Food & Nutrition, Duksung Women's University)
Kang, Keum-Jee (Dept. of Food & Nutrition, Duksung Women's University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.12, 2011 , pp. 1654-1661 More about this Journal
Abstract
We investigated the effect of Angelica keiskei ethanol (AKE) extract on cell death in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the presence 125, 150 and 175 ${\mu}g$/mL concentrations of AKE for 24 hours. MTT assays demonstrated that mitochondrial dehydrogenase activities decreased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In contrast, the proportion of dual staining with Hoechst 33342/ethidium bromide(EtBr) for cell death increased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In particular, the levels of cell death caused by apoptotic program showed marked increases in the 150 and 175 ${\mu}g$/mL AKE groups, as revealed by flow cytometry. An apoptotic suppressor gene, Bcl-2, significantly decreased at the transcript level (p<0.05). The expression levels of proapoptotic genes, both Bax and caspase 3 significantly increased (p<0.05). Furthermore, the ratio of Bcl-2/Bax mRNA which is considered to be an important indicator of apoptosis, significantly decreased in a dose-dependent manner (p<0.05). These results taken together indicate that, the AKE extract used in this study induces cell death in MDA-MB-231 human breast cancer cells.
Keywords
Angelica keiskei; MDA-MB-231 cell; apotosis; Bcl-2; Bax; caspase-3;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Yin XM, Oltvai Z, Korsmeyer SJ. 1994. BH1 and BH2 domains of bcl-2 are required for inhibition of apoptosis and heterodimerization with bax. Nature 369: 321-323.   DOI
2 Kluck RM, Bossy-Wetzel E, Green DR, Newmeyer DD. 1997. The release of cytochrome c from mitochondria: a primary site for bcl-2 regulation of apoptosis. Science 275: 1132-1136.   DOI   ScienceOn
3 Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J. 1997. Prevention of apoptosis by bcl-2: release of cytochrome c from mitochondria blocked. Science 275: 1129-1132.   DOI
4 Yu MH, Im HG, Hwangbo MH, Lee JW, Lee IS. 2005. Induction of apoptosis by immature Prunus salicina Lindi. cv. Soldam. Korean J Food Sci Technol 37: 221-227.
5 Shimizu E, Hayashi A, Takahashi R, Aoyagi Y, Murakami T, Kimoto K. 1999. Effects of angiotensin I-converting enzyme inhibitor from Ashitaba (Angelica keiskei ) on blood pressure of spontaneously hypertensive rats. J Nutr Sci Vitaminol 45: 375-383.   DOI
6 Heo BG, Chon SU, Park YJ, Bae JH, Park SM, Park YS, Jang HG, Gorinstein S. 2009. Antiproliferative activity of Korean wild vegetables on different human tumor cell lines. Plant Foods Hum Nutr 64: 257-263.   DOI
7 Enoki T, Ohnogi H, Nagamine K, Kudo Y, Sugiyama K, Tanabe M, Kobayashi E, Sagawa H, Kato I. 2007. Antidiabetic activities of chalcones isolated from Japanese herb, Angelica keiskei. J Agric Food Chem 55: 6013-6017.   DOI
8 Comes F, Matrone A, Lastella P, Nico B, Susca FC, Bagnulo R, Ingravallo G, Modica S, Lo Sasso G, Moschetta A, Guanti G, Simone C. 2007. A novel cell type-specific role of p38 alpha in the control of autophagy and cell death in colorectal cancer cells. Cell Death Differ 14: 693-702.   DOI
9 Hoshino Y, Mio T, Nagai S, Miki H, Ito I, Izumi T. 2001. Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line. Am J Physiol Lung Cell Mol Physiol 281: 509-516.   DOI
10 Sun J, Liu RH. 2006. Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells. Cancer Lett 241: 124-134.   DOI
11 Kobayashi T, Ruan S, Clodi K, Kliche KO, Shiku H, Andreeff M, Zhang W. 1998. Overexpression of Bax gene sensitizes K562 erythroleukemia cells to apoptosis induced by selective chemotherapeutic agents. Oncogene 16: 1587-1591.   DOI
12 Oltvai ZN, Milliman CL, Korsmeyer SJ. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619.   DOI
13 Yang E, Zha J, Jockel J, Boise LH, Thompson CB, Kosmeyer SJ. 1995. Bad, a heterodimeric partner for bcl-XL and bcl-2, displaces bax and promotes cell death. Cell 80: 285-291.   DOI
14 Tabata K, Motani K, Takayanagi N, Nishimura R, Asami S, Kimura Y, Ukiya M, Hasegawa D, Akihisa T, Suzuki T. 2005. Xanthoangelol, a major chalcone constituent of Angelica keiskei , induces apoptosis in neuro blastoma and leukemia cells. Biol Pharm Bull 28: 1404-1407.   DOI
15 Kimura Y, Baba K. 2003. Antitumor and antimetastatic activities of Angelica keiskei roots, part I: isolation of an active substance, xanthoangelol. Int J Cancer 106: 429-437.   DOI
16 Takaoka S, Hibasami H, Ogasawara K, Imai N. 2008. Chalcones from Angelica keiskei induce apotosis in stomach cancer cells. J Herbs Spices Mes Plants 14: 166-174.   DOI   ScienceOn
17 Hsu YL, Taeng WS, Kuo PL, Lin CC. 2006. Chalcone inhibits the proliferation of human breast cancer cell by blocking cell cycle progression and inducing apoptosis. Food Chem Toxicol 44: 704-713.   DOI
18 Chuang JY, Huang YF, Lu HF, Ho HC, Yang JS, Li TM, Chang NW, Chung JG. 2007. Coumarin induces cell cycle arrest and apoptosis in human cervical cancer heLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-${\kappa}B$ down-regulation. In Vivo 21: 1003-1009.
19 Li L, Aldini G, Carini M, Chen CYO, Chun HY, Cho SM, Park KM, Correa CR, Russell RM, Blumberg JB, Yeun KJ. 2009. Characterisation, extraction efficiency, stability and antioxidant activity of phytonutrients in Angelica keiskei. Food Chem 115: 227-232.   DOI
20 Ogawa H, Okada Y, Kamisako T, Baba K. 2007. Beneficial effect of xanthoangelol, a chalcone compound from Angelica keiskei , on lipid metabolism in stroke-prone spontaneously hypertensive rats. Clin Exp Pharmacol Physiol 34: 238-243.   DOI
21 Fujita T, Sakuma S, Sumiya T, Nishida H, Fujimoto Y, Baba K, Kozawa M. 1992. The effects of xanthoangelol E on arachidonic acid metabolism in the gastric antralmucosa and platelet of the rabbit. Res Commun Chem Pathol Pharmacol 77: 227-240.
22 Clarke PG, Clarke S. 1995. Historic apoptosis. Nature 378: 230.   DOI
23 Ministry of Health and Welfare. 2008. National survey of cancer patients: Analysis of national cancer survey. Seoul, Korea. p 10.
24 Kang HG, Kim HS, Lee MR, Seol SM, Oh JH, Lee SH, Kang GH. 2005. Expressions of apoptotic gene (survivin, bcl-2, bax) and clinical relevance in osteosarcoma. J Kor Bone J oint Tumor Soc 11: 118-125.
25 American Institute of Cancer Research/World Cancer Research Fund. 2007. Food, nutrition, physical activity, and prevention of cancer: a global perspective. American Institute of Cancer Research, Washington, DC, USA. p 4-29.
26 Alnermri ES. 1997. Mammalian cell death proteases: a family of highly conserved aspartate specific cystein proteases. J Cell Biochem 64: 32-42.
27 Zhap ZQ, Budde JM, Morris C, Wang NP, Velez DA, Murak S, Guyton RA, Vinten JJ. 2001. Adenosine attenuated reperfusion induced apoptotic cell death by modulation expression of Bcl-2 and Bax proteins. J Mol Cell Cardiol 33: 57-68.   DOI
28 Kim OK, Lee MW, Park WB, Ham SS, Kung SS. 1992. The nutritional components of aerial whole plant and juice of Angelica keiskei Koidz. Kor J Food Sci Technol 24: 592-596.   과학기술학회마을
29 Toyoda M, Tanaka K, Hoshino K, Akiyama H, Tanimura A, Saito Y. 1997. Profiles of potentially antiallergic flavonoids in 27 kinds of health tea and green tea infusions. J Agric Food Chem 45: 2561-1564.   DOI
30 Okuyama T, Takata M, Takayasu J, Hasegawa T, Tokuda H, Nishino A, Nishino H, Iwashima A. 1991. Anti-tumorpromotion by principles obtain from Angelica keiskei. Planta Med 57: 242-246.   DOI