• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.475-483
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• 2012

Tribolodon hakonensis(Cypriniformes; Leuciscinae) is anadromous; they are born in freshwater, migrate back to the ocean, then return to their home stream for spawning from mid-March to early-June. Here, five microsatellites were used to assess the level of gene flow among T. hakonensis populations from the Samcheok-Oship Stream, South Korea. The frequencies of dominant alleles across several loci differed between down-and upstream populations divided by several weirs, and pairwise multilocus $F_{ST}$ estimate was significantly high(0.083). However, there were no signs of any loss of genetic variation in the upstream population. Assignment tests of individuals in admixture model(K=2) to a set of baseline samples showed fairly correct assignment to each cluster; all of upstream individuals sere included in the first cluster, while the majority of downstream individuals(65%) comprise the second cluster. These results indicate reduced gene flow between up- and downstream populations but allowing passive downstream drift. It is likely that man-made structures might at least partially be a factor for creating and consolidating the current distribution patterns of genetic variation among T. hakonensis populations in the Samcheok-Oship Stream. This information will assist governing agencies in making informed decisions regarding conservation of anadromous fishes in Korean drainage systems.

• Journal of Life Science
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• v.19 no.9
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• pp.1183-1189
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• 2009

85 individual Spiraea prunifolia for. simpliciflora (Rosaceae) were sampled to examine the genetic diversity and population structure of S. prunifolia for. simpliciflora populations. Inter-simple sequence repeats (ISSR) produced 65 polymorphic loci and identified 78 ISSR genotypes. Three multilocus genotypes were shared by more than one plant within a population. Total genetic diversity values ($H_T$) and inter-locus variation in the within-population genetic diversity ($H_S$) were 0.293 and 0.183, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.373. This indicated that about 37.3% of the total variation was among populations. ISSR markers are very effective in classifying natural population levels of S. prunifolia for. simpliciflora in Korea. In addition, insights into the relative gene diversity among and within populations of S. prunifolia for. simpliciflora would be useful in plant breeding and also for the development of strategies for ex situ conservation of plant genetic resources.

• Korean Journal of Environment and Ecology
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• v.31 no.5
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• pp.461-471
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• 2017

Population genetic studies of 10 groups of Iksookimia pacifica were conducted to investigate the genetic diversity and population genetic structure across its known range in South Korea. Population DNA sequences of one mitochondrial gene (mtCOI) and three nuclear genes (IRBP, EGR2B, RAG1) were examined in samples collected from ten streams that flow into the East Sea. Both mitochondrial and nuclear sequences exhibited significant differentiation among populations except a few cases. The Bayesian analysis of the multi-locus genotypes inferred from the DNA sequences of nuclear genes clustered the individual fish largely into two geographical groups: a northern group (from Baebong stream to Cheonjin stream) and a southern group (Yangyangnamdae stream to Gangneungnamdae stream). Given that the streams flowing into the East Sea are geographically isolated water systems, such separation of genotypes can be interpreted by the geographical separation of common ancestors into north and south that had colonized South Korea. Since the initial geographical separation of the ancestral population by north and south, the ancestral groups seem to have experienced further differentiation into the current genetic clusters through the physical isolation of streams by the East Sea in each region. It is notable that many individuals in the Jasan stream formed a genetic cluster with those of Yangyangnamdae and Gangneungnamdae streams which are distant from each other. In addition, mitochondrial gene showed low genetic differentiation between some neighboring populations and very low level of genetic diversity in several populations. The present population genetic study will provide valuable information for the conservation and management of the Korean endemic fish species, I. paicifica.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.882-888
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• 2007

Enzyme electrophoresis was used to estimate genetic diversity and population genetic structure of Pseudobagrus fulvidraco in Korea. Nine of the 14 loci (64.3%) showed detectable polymorphism. Genetic diversity at the population and species levels were 0.286 and 0.277, respectively. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a substantial deficit of hetero-zygotes relative to Hardy-Weinberg expectations. This deficit is expected that it is due to a limited effective number of individuals per population. The average $G_{ST}$ for polymorphic loci was 0.064, indicating that most (93.6%) of the genetic diversity occurred within populations. The indirect estimate of gene flow based on mean $G_{ST}$ was 3.67. Given limited gene flow is expected to diverge genetically due to drift and reduced populations. Most populations in our study experience annual, severe demo-graphic bottlenecks due to drought and floods.

• Journal of Mushroom
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• v.18 no.4
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• pp.323-330
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• 2020

Agaricus bisporus is an important edible mushroom that is used as a functional food. In this study, European A. bisporus strains were analyzed for genetic diversity, population structure, and genetic differentiation using simple sequence repeat (SSR) markers. European A. bisporus strains were divided into four groups by distance-based analysis and two subpopulations by model-based analysis. The SSR markers used in this study did not group European A. bisporus strains by geographical region or pileus color. Genetic diversity was high in Group 4 based on distance-based analysis and Pop. 2 based on model-based analysis. A. bisporus strains showed very low genetic differentiation. The results of this study can be used for breeding A. bisporus in the future.

• Journal of Life Science
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• v.23 no.6
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• pp.728-735
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• 2013

Plantago asiatica (Plantaginaceae) is a wind-pollinated plant that grows mainly on fields in East Asia. Starch gel electrophoresis was used to investigate the allozyme diversity and population structure of 18 populations of this species. Although the plantain populations were isolated and patchily distributed, they maintained a high level of genetic diversity; the average percentage of polymorphic loci was 57.1%, the mean number of alleles per locus was 2.07, and the average heterozygosity for 18 populations was 0.201. The combination of a predominant wind-pollinated, mix-mating reproduction, large population sizes, high gene flow between subpopulations, and a propensity for high fecundity may explain the high level of genetic diversity within populations. A direct gradient in overall genetic diversity is associated with latitude. Genetic diversity of P. asiatica is markedly decreased from $35^{\circ}3^{\prime}$ to high latitude and decreased from $35^{\circ}3^{\prime}N$ to low latitude, whereas there does not show a longitudinal gradient in genetic diversity.

• Journal of Life Science
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• v.16 no.6
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• pp.898-903
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• 2006

The genetic diversity and population structure of fifteen Potentilla discolor Bunge populations in Korea were determined using genetic variations at 19 allozyme loci. Fourteen of the 19 loci (73.7%) showed detectable polymorphism. Genetic diversity at the species level and at the population level was high ($H_{ES}\;=\;0.215$, $H_{EP}\;=\;0.196$, respectively), whereas the extent of the population divergence was relatively low $(G_{ST}\;=\;0.069)$. Total genetic diversity values $(H_T)$ varied between 0.0 and 0.656, giving an average overall polymorphic loci of 0.292. The interlocus variation of genetic diversity within populations $(H_S)$ was high (0.274). On a per locus basis, the proportion of total genetic variation due to differences among populations $(G_{ST})$ ranged from 0.010 for Pgm-2 to 0.261 for Pgd-2 with a mean of 0.069, indicating that about 6.9% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. discolor. The estimate of gene flow based on $G_{ST}$ was high among Korean populations of P. discolor (Nm = 3.36).

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.72-72
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• 2022

자두나무(Prunus salicina Lindl.)는 세계에서 5번째로 많이 생산되는 과실로, 한국에서 재배하는 자두나무의 기본종은 중국 양쯔강 유역에서 기원했다. 2016년 과수용 자두나무와는 다른 자두나무가 양구에서 발견되었다. 양구군, 인제군, 고성군 일대의 자두나무 개체군의 유전다양성 및 집단 구조 분석을 통해 본 자두나무 개체군의 유전다양성 및 개체군 구조를 확인하고자 했다. 과수용 재배종을 포함한 시료를 채취하여 GBS 분석을 진행했고 주성분 분석과 STRUCTURE 분석을 통해 개체군간 유전적 구조를 확인했다. 재배종의 유전형이 다른 개체군에서도 나타나는 것으로 보아 유의한 유전적 분화가 일어났다고 보기 힘들었다. 하지만 고성군 고진동계곡 등 DMZ에 인접한 집단이 분계도에서 재배종 개체군과 가장 유전적 거리가 있는 것으로 나타났다. 하지만 본 분석으로는 야생집단으로 발견도니 자두나무의 실체와 기원을 단정짓기에는 부족한 것으로 보이며 외군 추가 및 더 많은 시료를 확보하는 등 추가 조사와 분석이 필요하다.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.29 no.4
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• pp.142-149
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• 2018

In this study, 844 Hanwoo proven bulls in South Korea (called KPN) were classified into 8 groups based on their birth year. Microsatellite (MS) marker information for paternity identification of each individual is provided at the homepage of the National Agricultural Cooperation Federation, Korea (NACF) and is mainly for the analysis of genetic diversity and structural changes. The polymorphism analysis of KPN whole groups revealed the average number of alleles in each marker (number of alleles), the expected heterozygosity ($H_{ex}$), the observed heterozygosity ($H_{ob}$), the polymorphism information content (PIC) and the $F_{is}$ mean as 10.54, 0.764, 0.773, 0.727 and -0.014, respectively. For group D, with the birth year 2004-2005, the $H_{ex}$ and $H_{ob}$ were 0.777 and 0.792 respectively and the PIC was 0.740. The $H_{ex}$ of group C and D, with birth years 2003-2004 and 2007-2008, respectively, were greater than $H_{ob}$. In all the other groups, $H_{ob}$ was greater than $H_{ex}$. Genetic composition and structure were analyzed using STRUCTURE software. According to the analyzed results, the generation of Hanwoo groups showed changes in specific genetic components according to the flow. It was confirmed that the continuous improvement in the Hanwoo affects the genetic structure of the proven bulls group. The results of this study are expected to be used for enhancing the efficiency of Hanwoo improvement project.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.877-881
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• 2007

The genetic diversity and population structure of Potentilla freyniana in Korea were determined using genetic variations at 19 allozyme loci. Thirteen of the 19 loci (68.4%) showed detectable polymorphism. Genetic diversity at the population level was high ($H_{EP}$ = 0.270). Total genetic diversity values ($H_T$) varied between 0.190 and 0.584, giving an average overall polymorphic loci of 0.371. The interlocus variation of genetic diversity within populations ($H_S$) was high (0.354). On a per locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.008 for Fe-2 to 0.310 for Gpi with a mean of 0.065, indicating that about 6.5% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. freyniana. The estimate of gene flow based on $G_{ST}$, was high among Korean populations of P. freyniana (Nm =3.57). Although P. freyniana usually propagated by asexually-produced ramets, I could not rule out the possibility that sexual reproduction occurred at a low rate because each ramet may produce terminal flowers.