• Korean Journal of Plant Resources
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• v.34 no.2
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• pp.156-165
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• 2021

The in vitro regeneration was established, and the genetic stability among the mother plants (control) and the micropropagated green plants was evaluated using ISSR markers in Imperata cylindrica 'Rubra', Poaceae which containing important bioenergy plants. Green shoots were multiply induced from growing point culture via callus on MS medium supplemented with 0.01 mg/L NAA and 2 mg/L BA, and the shoots were proliferated on the MS medium with rooting. Rooted plantlets were transplanted to the pot with 100% survival rate. Using ISSR markers, somaclonal variation was analyzed in eight mother plants (control), ten green-regenerant cultivated at culture room (ReR) and ten green-regenerant cultivated at field condition (ReF). All ISSRs produced a total of 97 bands, and the scorable bands varied from one to seven with an average of 4.4 bands per primer. The polymorphism rate of ReRs and ReFs was 4.1% and 3.1% respectively, showing higher rate than that of control (0%). The genetic similarity matrix (GSM) among all accessions ranged from 0.919 to 1.0 with a mean of 0.972. According to the clustering analysis, ReFs and mother plants were divided into two independent groups. The results indicate that no clear genetic diversity was detected among regenerated plants, and ISSR markers were useful tool for identification of somaclonal variation of regenerants.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.235-243
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• 2001

Genetic variation of the beet armyworm, Spodoptera exigua (Hubner), was analyzed by polymorphic allozymes. Field populations were subdivided by different hosts, geographical locations, and seasons. Estimated average heterozygosity ($0.443\pm$0.013) indicated high genetic variation in all field populations of S. exigua. There were significant inbreeding effects deviated from Hardy-Weinberg equilibrium in each of subpopulations. These significant nonrandom matings were caused by within-subpopulations probably due to sampling errors, but not by mating isolation among subpopulations. Wrights ($F_{ST}$ ) and Neis (D) genetic distances indicated little genetic differentiation among subpopulations, though some southern local subpopulations (Haenam and Sachon) were relatively different of northern subpopulations (Andong and Kunwi). Estimated number of migrants per generation was 5.9 among host subpopulations, 10.6 among geographical subpopulations, and 31.8 among seasonal subpopulations. These genetic analyses suggest that Korean S. exigua subpopulations have little genetic differentiation mostly due to their significant migratory capacity.

• Animal Systematics, Evolution and Diversity
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• v.13 no.1
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• pp.9-20
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• 1997

한국산 송사리 (Oryzias)속의 송사리 (O. latipes)와 대륙송사리(O. sinensis)의 집단 내 유전적 변이 및 종간 유연관계를 밝히고자 남한의 16개집단과 일본산 송사리(O. latipes) 1개 집단 등 총 17개 집단에 대한 isozyme 분석을 실시하였다. Isozyme 분석 결과 17개의 효소 및 비효소단백질에서 총 32개의 유전자를 검출한 결과, 전체 유전자중 Est-1과 Est-3 는 종 특유의 유전적 표식인자(genetic marker)였다. 송사리집단 중 진도집단의 유전적 변이 가 가장 낮았으며(Ho=0.011, He=0.043), 대륙송사리 서천집단의 유전적 변이가 가장 높았 다.(Ho=0.114, He=0.124). 한국산 송사리 11개 집단의 평균 유전적 변이 정도는 P=12.7%, Ho=0.029, He=0.042, 대륙송사리 5개 집단의 평균 유전적 변이정도는 P=26.3%, Ho=0.082, He=0.097로 각각 나타나 대륙송사리집단의 유전적 변이 정도가 송사리집단에 비해 약 2.5배 정도 높았고 일본산 송사리의 유전적 변이 정도는 P=15.6%, Ho=0.073, He=0.068로 나타났 다. 한국산 송사리와 일본산 송사리와의 유연관계는 S=0.761(D=0.243)로 나타나 유전적으로 뚜렷한 별개의 분류군으로 생각되며, 대륙송사리와 송사리간의 종간 유전적 근연치는 S=0.648(D=0.389)로 일반적인 척추동물의 종간 유전적 분화 수준을 나타냈다.

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.414-421
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• 2016

Fourteen Acer pseudosieboldianum populations in South Korea were used to estimate genetic diversity, genetic differentiation and genetic relationships using seven AFLP primer combinations. The average of effective alleles ($A_e$), the proportion of polymorphic loci (%P) and Shannon's diversity index (I) was 1.4, 82.2% and 0.358, respectively. The expected heterozygosity ($H_e$) under Hardy-Weinberg equilibrium was 0.231 and the expected heterozygosity (Hj) from Bayesian inference was 0.253. The level of genetic diversity was moderate compared to those of Genus Acer and lower than those of other species having similar ecological niche and life history. The inbreeding coefficient within populations ($F_{IS}$) from Bayesian method was 0.712 and it could be influenced by selfing or biparental inbreeding to induce homozygote excess. The level of genetic differentiation was 0.107 from AMOVA (${\Phi}_{ST}$) and 0.110 from Bayesian method (${\Phi}^{II}$). The genetic differentiation was lower than those of other species having similar ecological niche and life history. Ulleungdo population had the lowest level of genetic diversity and was genetically the most distinct population from others in the study. We consider that founder effect and genetic drift might be occurred to reduce genetic diversity and then the geographical isolation might interrupt gene flow to aggravate it.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.320-327
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• 2013

This study was carried out to address an efficient in vitro regeneration system from seed-derived callus of Phragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration via calli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6 supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 loci detected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of different in vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2 mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect genetic variation of regenerants.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.577-584
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• 2011

To estimate level of genetic variation and genetic differentiation among populations of 3 populations in Abies koreana and 5 populations in Abies nephrolepis, 5 nSSR markers were analyzed. Except 1 locus where too many alleles were observed excessively, population genetic parameters were recalculated with 4 loci. Mean expected heterozygosities ($H_e$) were 0.292 in A. koreana and 0.220 in A. nephrolepis, respectively. In both species, positive fixation coefficient was estimated (F=0.065 for A. koreana and F=0.095 for A. nephrolepis), which suggests that there is an excess of homozygotes relative to Hardy-Weinberg expectations within populations. Relatively high degree of population differentiation was observed in A. koreana ($F_{ST}=0.063$). compared to that of A. nephrolepis ($F_{ST}=0.039$). From 3-level Hierarchical estimation of F-staticstics, only 4.9% of the genetic variation was allocated between species ($F_{PT}$), which suggested that most of genetic variation was shared between two species. On the basis of results from analysis of genetic relationships among populations, 2 populations of A. koreana (Mt. Halla and Mt. Deogyu) were genetically distinct from the populations of A. nephrolepis but a population of Mt. Jiri was allocated within a group of populations of A. nephrolepis. Populations of both species seemed to have undergone genetic drift due to gradual decrease in population size induced by global warming after the last glacier, which resulted in increase of homozygotes by inbreeding. It could be also postulated that these species might be diverged recently and It is likely that the two species have not fully speciated yet.

• Korean Journal of Environment and Ecology
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• v.11 no.3
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• pp.277-289
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• 1997

Electrophresis methods were used to examined the degree of genetic relatedness and divergent times on the 4 genera 4 species of the family Corvidae and 1 species of the Family Cinclidae. Genetic relatedness between two families and between 4 genera of Corvidae showed similar to those reported at comparable taxonomic levels in other bird. But it appears to be considerably less than that of non avian taxa. Genetic relatedness between Garrulus glandarius brandtii and Corvus corone orientalis was the most close related(S=0.81), whereas Pica pica sericea(S=0.72) and Cyanopica cyanus koreensis(S=0.68) were showed less close relatedness respectively. The presumed divergent time between two families was 3.9 million years before present. Whereas among the 4 species of corvidae, the presumes divergent times of Cyanopica cyanus koreensis and Pica pica sericea were about 1.9 and 1.6 million years before present respectively, and between Garrulus glandarius brandtii and Corvus corone orientalis was showed recently about 1.0 million years before present

• Korean Journal of Plant Taxonomy
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• v.43 no.3
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• pp.236-243
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• 2013

Many plant species in subalpine regions are under threat of extinction as a result of climate change. In this study, the genetic diversity and geographic differentiation of three regions and six populations of Primula farinosa subsp. modesta (Bisset & Moore) Pax in Korea were assessed using the ISSR (Inter Simple Sequence Repeat) marker. The average genetic diversity (P = 60.62, SI = 0.299, h = 0.190) was relatively lower than that of other long-lived perennials, even though it is a self-incompatible species. AMOVA analysis showed that 50% of the total genetic diversity was partitioned among regions and Bayesian cluster analysis showed some remarkable geographic trends that were structured into 2 or 3 regions, suggesting limited gene flow among regions. Considering the population fragmentation, low level genetic diversity, and high genetic differentiation, it is essential to establish in situ and ex situ conservation strategies for P. farinosa subsp. modesta.

• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.267-274
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• 2012

To understand morphological and genetic differentiation between Euphorbia esula and E. maackii we examined 12 morphological characters and 11 isozyme loci from 14 populations of two species. Species of E. esula complex (A = 1.63, P = 44.83, $H_e$ = 0.198) in Korea maintain nearly as high as the genetic diversity reported in East Asian E. jolkinii and E. fauriei while lower than those of E. ebracteolata and E. pekinensis in Korea. Although the ranges of most morphological character variation of the two species overlap, E. esula and E. maackii were well recognized by the combination of the morphological traits, and the result of UPGMA phenogram supports the two distinct species inhibited in Korea. However, isozyme data do not support the recognition of E. esula and E. maackii. The discordance between morphological and allozyme data should be explained by the recent divergence or gene flow via introgressive hybridization between two species.

• Korean Journal of Plant Taxonomy
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• v.45 no.1
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• pp.1-7
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• 2015

Using starch gel electrophoresis, ten isozyme loci were examined in nine populations of Euphorbia ebracteolata in Korea. Populations of E. ebracteolata tend to have higher within-population levels of genetic variation (A = 2.2, P = 61.1, He = 0.165) along with low levels of genetic differentiation ($F_{ST}$ = 0.075) compared to previously reported Euphorbia species. The relatively high genetic variation in E. ebracteolata may be due to its long-lived regional distribution and to the outcrossed nature of the species. Low levels of genetic differentiation and the absence of unique alleles in E. ebracteolata populations suggest that Korean populations diverged recently. This study does not support the recognition of var. coreana and for. magna as separate taxa, and suggests that they should be combined with E. ebracteolata.