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http://dx.doi.org/10.7732/kjpr.2013.26.2.320

Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius)  

Ryu, Jaihyunk (Department of Bioresources Science, Sunchon National University)
Kim, En-Hwan (Department of Bioresources Science, Sunchon National University)
So, Hyun-Su (Department of Bioresources Science, Sunchon National University)
Chung, Mi-Young (Department of Agricultural Education, Sunchon National University)
Song, Won-Seob (Department of Horticulture, Sunchon National University)
Bae, Chang-Hyu (Department of Bioresources Science, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.26, no.2, 2013 , pp. 320-327 More about this Journal
Abstract
This study was carried out to address an efficient in vitro regeneration system from seed-derived callus of Phragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration via calli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6 supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 loci detected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of different in vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2 mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect genetic variation of regenerants.
Keywords
ISSR; Genetic diversity; Phragmites communis Trinius; Regeneration;
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Times Cited By KSCI : 6  (Citation Analysis)
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