• Title/Summary/Keyword: 유용세균

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Recycling Marine Fish Farm Effluent by Microorganisms (유용미생물을 이용한 육상수조식 양식장 배출물의 재활용)

  • 문상욱;이준백;이영돈;김세재;강봉조;고유봉
    • Journal of Aquaculture
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    • v.15 no.4
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    • pp.261-266
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    • 2002
  • The effluent sediment from the land-based seawater fish farms of Jeju consists of proteins, fats, ash and moisture. An evaluation of the effluent sediment as substrate for growth of phototrophic or lactic acid bacteria revealed that the sediment supported the growth of phototrophic bacteria but could support lactic acid bacteria only on supplementation with sugar. The possibility of using phototrophic bacteria for recycling the land-based seawater fish farm effluent is shown.

Biological Control of Soft Rot on Chinese Cabbage Using Beneficial Bacterial Agents in Greenhouse and Field (유용세균(Beneficial Bacterial Agents)을 이용한 배추 무름병의 생물적 방제)

  • Shrestha, Anupama;Kim, Eun-Chang;Lim, Chuen-Keun;Cho, Sae-Youll;Hur, Jang-Hyun;Park, Duck-Hwan
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.325-331
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    • 2009
  • Three beneficial bacterial agents, Lactobacillus strain KLF01, Lactococcus strain KLC02 and Paenibacillus strain KPB3 were showed clear zone against plated Pectobacterium carotovorum subsp. carotovorum (Pcc) soft rot pathogen. In greenhouse test, bio-control efficacy was more significantly effective in the treatments by KLC02 and KPB3 as 64%, 50%, 56% and 66%, 57%, 58% according to date of evaluation, respectively. In case of KLF01 control effect was relatively lower than treatments of KLC02 and KPB3 but its efficacy was still significantly observed when compared to control (only water treatment). Furthermore, KLF01, KLC02 and KPB3 showed 55%, 60% and 62% bio-control efficacy, respectively in field test from early March to late July of 2009. Thus, we suggest that these strains can be useful as bio-control agents against soft rot caused by Pcc.

한국형 유산균의 in vitro에서 장내유해효소의 억제효과

  • 박혜영;김동현;배은아;한명주
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1997.04a
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    • pp.83-83
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    • 1997
  • 사람의 장내에 서식하는 장내세균은 사람의 건강을 지켜주는 유용균과 나쁜 영향을 주는 유해균이 있으며 이들 양자의 균형에 의하여 건강상태가 조절되고 있다. 사람의 건강에 대단히 중요한 역할을 하는 유용균의 대표적인 것이 Bifidobacterium이다. 이 Bifidobacterium은 장내에서 lactic acid 및 acetic acid를 생산하여 장내의 pH를 산성으로 유지시키고 부패균의 증식을 억제하는 역할을 하며 신체를 유해세균의 작용으로부터 방어하는 역할을 하고 있다. 현재 우리나라에서 사용하고 있는 Bifidobacterium은 대부분 외국에서 개발된 것으로 한국인에게 어느 정도 효과가 있는지에 대해서는 아직 정확한 보고가 없으며 한국인의 장내균총으로부터 유산균이 분리된다면 한국인에게 가장 잘 정착될 수 있는 균주일 것으로 생각된다.

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The Destruction of Bacterial Spores Upon Compressional Pressure (타정 압력에 의한 세균포자의 파괴현상)

  • Lee, Cherl-Ho;Kim, Young-Man;Lee, Jung-Chi;Jung, Pil-Keun
    • Korean Journal of Food Science and Technology
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    • v.12 no.4
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    • pp.272-277
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    • 1980
  • The tolerance of useful bacterial spores to the conditions of tablet making, specifically, the destruction of bacterial spores upon compressional pressure was investigated. The damage of bacterial spores occurred mainly during the tabletting. The bacterial spores obeyed a logarithmic destruction rate upon compressional pressure. The spore destruction rate was dependent upon the strains of microorganism. The Decimal Reduction Pressure, designated as P-value, were $2.9\;ton/cm^2$, $2.6\;ton/cm^2$ and $2.1\;ton/cm^2$ for the spores of Bacillus subtilis, Bacilus coagulans and Clostridium butyricum, respectively, and $1.7\;ton/cm^2$ for the vegetative cell of Streptococcus faecalis. The spore destruction upon compressional pressure was influenced by the type of filler. The P-value of the spore of B. coagulans was $2.8\;ton/cm^2$ in the lactose filler, but $2.0\;ton/cm^2$ in the starch filler. The number of viable spores was inversely proportional to the hardness and density of tablet, in case that the same type of filler was used. The starch filler, which resulted in the lower hardness and lower density of tablet, caused higher spore destruction rate compared with the lactose filler.

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Comparative Analysis of Immunosuppressive Metabolites Synthesized by an Entomopathogenic Bacterium, Photorhabdus temperata ssp. temperata, to Select Economic Bacterial Culture Media (곤충병원세균(Photorhabdus temperata ssp. temperata) 유래 곤충 면역 억제물질 생성 비교 연구를 통한 저렴한 세균 배지 선발)

  • Seo, Sam-Yeol;Jang, Ho-Jin;Kim, Kun-Woo;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.49 no.4
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    • pp.409-416
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    • 2010
  • An entomopathogenic bacterium, Photorhabdus temperata ssp. temperata (Ptt), suppresses insect immune responses and facilitates its symbiotic nematode development in target insects. The immunosuppressive activity of Ptt enhances pathogenicity of various microbial pesticides including Bacillus thuringiensis (Bt). This study was performed to select a cheap and efficient bacterial culture medium for large scale culturing of the bacteria. Relatively cheap industrial bacterial culture media (MY and M2) were compared to two research media, Luria-Bertani (LB) and tryptic soy broth (TSB). In all tested media, a constant initial population of Ptt multiplied and reached a stationary phase at 48 h. However, more bacterial colony densities were detected in LB and TSB at the stationary phase compared to two industrial media. All bacterial culture broth gave significant synergism to Bt pathogenicity against third instars of the diamondback moth, Plutella xylostella. Production of bacterial metabolites extracted by either hexane or ethyl acetate did not show any significant difference in total mass among four culture media. Reverse phase HPLC separated the four bacterial metabolites, which were not much different in quantities among four bacterial culture broths. This study suggests that two industrial bacterial culture media can be used to economically culture Ptt in a large scale.

Aerobic Bacteria Image Management System (호기성 세균 화상 관리 시스템)

  • Koo Bong-Oh;Shin Yong-Won;Park Byung-Rae
    • The Journal of the Korea Contents Association
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    • v.5 no.1
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    • pp.37-44
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    • 2005
  • In this study, we integrated 40 kinds of aerobic bacteria that has a higher appearance rate and data for experts and educations, and constructed the aerobic bacterial images database. Constructed system is useful to culture of bacteria for novice without expert's heuristics and deep knowledge and to decrease time and money for handling of patient's examination results. Moreover, it can add new bacterial information in database and contribute to raise a medical quality and it is useful to support a expert's technology in department of laboratory medicine.

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Rapid Microbiological Assessment Method by using ATP-Bioluminescence in Ginseng Powder (ATP-Bioluminescence를 이용한 인삼분말의 미생물 신속검사법)

  • 곽이성;김천석;송용범;고셩룡
    • Journal of Ginseng Research
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    • v.25 no.3
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    • pp.127-129
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    • 2001
  • Bioluminescence technique was applied to ginseng powders. ATP bioluminescence can be used as a rapid method that can be implemented for microbiological monitoring of contaminated ginseng powders. The RLU (relative light units) of ATP was proportion to bacterial CFU (colony forming units) when in high contaminated ginseng powders ($\geq$ about 1.0$\times$10$^4$CFU/g). However, when in low contaminated ginseng powders ($\times$10$^4$CFU/g), the RLU was not proportion to CFU, respectively.

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Comparison of Automated Systems for Identification of Vibrio Species (Vibrio 속 세균 동정에 대한 자동화동정 시스템의 비교)

  • 박진숙;권주리
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.62-66
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    • 2002
  • Twenty one Vibrio strains were isolated from costal water and their phenotypic properties were determined. We identified bacterial isolates by using automated identification systems, Vitek and MIDI, and compared their identification results with each other. The comparison of them provided identical species information for only one isolates, TL33( V. alginolyticus). From Vitek and MIDI,16 (76%), and 6 (29%) out of 21 isolates were identified to the species level, respectively. Vitek was more useful than MIDI for identification of Vibrio species.

Characterization of Algal-Bacterial Ecological Interaction and Nutrients Removal Under Municipal Wastewater Condition (실제 하수조건에서 조류-세균 복합군집의 생태적 상호작용 및 영양염류 제거 특성 규명)

  • Lee, Jang-Ho;Park, Joon-Hong
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.5
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    • pp.314-324
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    • 2011
  • Algal biomass cultivated by wastewater is potentially useful resource for biodiesel production. However, little is known about algal nutrient metabolism and microbial interaction with bacteria under real municipal wastewater condition. In this work, we characterized nitrogen and phosphorus removals of municipal wastewater by a representative wastewater-growing algal population. Ankistrodesmus gracilis SAG 278-2, and analyzed its ecological interaction with wastewater bacterial communities. Compared to wastewater sludge itself, algal-bacterial co-culture improved nutrient removal. According to bacterial community analysis with 16S rRNA genes, a selective and dominant growth of a Unclassified Alcaligenaceae population resulted from algal growth in the algal-bacterial co-culture. The selectively stimulated bacterial population is phylogenetically close to Alcaligenes faecalis subsp. 5659-H, which is known to be co-present interact with algae in aquatic environment. These findings suggest that algal growth/metabolism may have effects on selection of a specific bacterial population in algal-bacterial co-cultures that can efficiently remove nutrients from municipal wastewater.