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Manufacturing of Monodisperse Pectin Hydrogel Microfibers Using Partial Gelation in Microfluidic Devices (미세유체 장치에서 부분젤화법을 이용한 단분산성 펙틴 하이드로젤 미세섬유의 제조)

  • Jin, Si Hyung;Kim, Chaeyeon;Lee, Byungjin;Shim, Kyu-Rak;Kim, Dong Young;Lee, Chang-Soo
    • Clean Technology
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    • v.23 no.3
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    • pp.270-278
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    • 2017
  • This study introduces a method to easily fabricate highly monodisperse pectin hydrogel microfibers in a microfluidic device by using partial gelation. The hydrodynamic parameters between the pectin aqueous solution and the calcium ions containing oil solution are precisely controlled to form a stable elongation flow of the pectin aqueous solution, and partial gelation of the pectin aqueous solution is performed by the chelating of the calcium ions at the interface between the two phases. The partially gelled pectin aqueous solution is phase-separated from the oil solution in an aqueous calcium chloride solution outside the microfluidic device and is completely gelled to produce monodisperse pectin hydrogel microfibers. The thickness of the pectin hydrogel microfiber is controlled in a reproducible manner by controlling the volumetric flow rate of the initially injected pectin aqueous solution. The pectin hydrogel microfibers were 200 to 500 micrometers in diameter and had a coefficient of variation below 5% under all thickness conditions, indicating that the pectin hydrogel microfibers produced by partial gelation are highly monodisperse. In addition, biomaterials can be immobilized to the pectin hydrogel microfibers produced by a single process, demonstrating the possibility that our pectin hydrogel microfiber can be used as carriers for biomaterials or tissue engineering.

Quantitative Analysis of Bacillus amyloliquefaciens GR4-5 in Soil (Bacillus amyloliquefaciens GR4-5 균주의 토양 내 정량 분석)

  • Kim, Dayeon;Kim, Byung-Yong;Ahn, Jae-Hyung;Weon, Hang-Yeon;Kim, Sung-Il;Kim, Wan-Gyu;Song, Jaekyeong
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.847-858
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    • 2015
  • Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor - of which soil was put in 14 mL tube after treatment - and field environments. Soil samples were collected over a four-week period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g $soil^{-1}$ immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g $soil^{-1}$) was similar to that of the control (6.38 log copies g $soil^{-1}$). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.

A 3.2Gb/s Clock and Data Recovery Circuit without Reference Clock for Serial Data Communication (시리얼 데이터 통신을 위한 기준 클록이 없는 3.2Gb/s 클록 데이터 복원회로)

  • Kim, Kang-Jik;Jung, Ki-Sang;Cho, Seong-Ik
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.46 no.2
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    • pp.72-77
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    • 2009
  • In this paper, a 3.2Gb/s clock and data recovery (CDR) circuit for a high-speed serial data communication without the reference clock is described This CDR circuit consists of 5 parts as Phase and frequency detector(PD and FD), multi-phase Voltage Controlled-Oscillator(VCO), Charge-pumps (CP) and external Loop-Filter(KF). It is adapted the PD and FD, which incorporates a half-rate bang-bang type oversampling PD and a half-rate FD that can improve pull-in range. The VCO consists of four fully differential delay cells with rail-to-rail current bias scheme that can increase the tuning range and tuning linearity. Each delay cell has output buffers as a full-swing generator and a duty-cycle mismatch compensation. This materialized CDR can achieve wide pull-in range without an extra reference clock and it can be also reduced chip area and power consumption effectively because there is no additional Phase Locked- Loop(PLL) for generating reference clock. The CDR circuit was designed for fabrication using 0.18um 1P6M CMOS process and total chip area excepted LF is $1{\times}1mm^2$. The pk-pk jitter of recovered clock is 26ps at 3.2Gb/s input data rate and total power consumes 63mW from 1.8V supply voltage according to simulation results. According to test result, the pk-pk jitter of recovered clock is 55ps at the same input data-rate and the reliable range of input data-rate is about from 2.4Gb/s to 3.4Gb/s.

Seismic Behavior and Performance Evaluation of Uckling-restrained Braced Frames (BRBFs) using Superelastic Shape Memory Alloy (SMA) Bracing Systems (초탄성 형상기억합금을 활용한 좌굴방지 가새프레임 구조물의 지진거동 및 성능평가)

  • Hu, Jong Wan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.3
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    • pp.875-888
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    • 2013
  • The researches have recently progressed toward the use of the superelastic shape memory alloys (SMAs) to develop new smart control systems that reduce permanent deformation occurring due to severe earthquake events and that automatically recover original configuration. The superelastic SMA materials are unique metallic alloys that can return to undeformed shape without additional heat treatments only after the removal of applied loads. Once the superelastic SMA materials are thus installed at the place where large deformations are likely to intensively occur, the structural system can make the best use of recentering capabilities. Therefore, this study is intended to propose new buckling-restrained braced frames (BRBFs) with superelastic SMA bracing systems. In order to verify the performance of such bracing systems, 6-story braced frame buildings were designed in accordance with the current design specifications and then nonlinear dynamic analyses were performed at 2D frame model by using seismic hazard ground motions. Based on the analysis results, BRBFs with innovative SMA bracing systems are compared to those with conventional steel bracing systems in terms of peak and residual inter-story drifts. Finally, the analysis results show that new SMA bracing systems are very effective to reduce the residual inter-story drifts.

A Study of the Cooling Effect of an Evaporation-Type Cool Roof Fan (기화방열식 Cool Roof Fan의 냉풍효과에 대한 연구)

  • Kim, Yeong Sik;Chung, Hanshik;Jeong, Hyomin;Choi, Soon-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.40 no.3
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    • pp.191-200
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    • 2016
  • The ventilation effect of a ventilation system, which is classified as the forced ventilation and natural ventilation, is predominantly dependent on the combination of air supply and discharge. Perhaps the simplest ventilation is merely supplying the air as it is. However, to improve the indoor working environment during the summer, an air supply that is cooled to some extent has been widely adopted. Recently, a cooling method utilizing the vaporization of water was introduced. In this study, the performance of an evaporation-type air supply unit that was produced by Japan K-company and was installed in a shoe-manufacturing plant in Busan was investigated. The purpose of the experiment was to measure how much the supplied air could be cooled. From this experimental study, we confirmed that the evaporation-type air supply system is efficient, capable of improving the working environment during the summer while minimizing the energy cost.

UPnP-based QoSAgent for QoS-guaranteed Streaming Service in Home Networks (서비스 품질이 보장되는 홈 네트워크 스트리밍 전송을 위한 UPnP 기반의 QoSAgent에 대한 연구)

  • Lee Hyun-Ryong;Moon Sung-Tae;Kim Jong-Won;Shin Dong-Yun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.5B
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    • pp.430-441
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    • 2006
  • As the various A/V devices and home networks are delivered to users, home networks are changing to an entertainment network. It is expected that the required network bandwidth and the amount of usage of media content in home entertainment networks will be increased. Although the access networks and home networks becoming a high speed network, there remains the problems for QoS-guaranteed media content transfer in home networks. Also, in the home network, there can be network traffic caused by applications like video conferencing, video telephone, and VoIP(voice over IP) as well as inner network traffic of home network. Since media content transfer requires the real-time delivery, it is very important and basic requirement that is to transfer media content to A/V device user wants while keeping the media quality. Even though there are many middleware protocol for home networking, they provide basic device discovery and control or simple functions for QoS-guaranteed media content transfer that are not enough to provide QoS-guaranteed media transfer service that user wants. Thus, in this paper, we propose the technique based on UPnP(universal plug and play) protocol for QoS-guaranteed media content transfer in the home network. The proposed technique is compatible with UPnP and can be used with UPnP as additional functions. In this paper, we utilize VideoLAN application to verify the proposed technique. We add the additional modules that support the proposed technique's function to VideoLAN and we verify the its functions through various test scenarios.

Properties of Hydration Heat of High-Strength Concrete and Reduction Strategy for Heat Production (고강도 콘크리트의 수화열 특성 및 발열 저감대책에 관한 연구)

  • Jaung, Jae-Dong;Cho, Hyun-Dae;Park, Seung-Wan
    • Journal of the Korea Institute of Building Construction
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    • v.12 no.2
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    • pp.203-210
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    • 2012
  • Recently, the interest and demand for large-scale buildings and skyscrapers have been on the rise, and the performance of concrete is an area of high priority. Securing 'mass concrete and high strength concrete' is very important as a key construction technology. For high strength concrete, the high heat of hydration takes place inside the concrete because of the vitality of hydration in cement due to the large amount of powder, and leads to problems such as an increase of thermal stress due to the temperature difference with the outside, which results in cracks and slump loss. For this reason, measures to solve these problems are needed. This study aims to reduce the hydration heat of high strength concrete to control the hydration heat of mass concrete and high strength concrete, by replacing the type of admixture, The purpose of this study is to control the hydration heat of high strength concrete and mass concrete. Our idea for this purpose is to apply not only the types and contents of admixture but also incorporation mixing water to ice-flake. As a result of the test, the use of blast furnace slag and fly ash as admixture, and the use of ice-flake as mixing water can improve the liquidity of concrete and reduce slump loss. Significantly dropping the maximum temperature will contribute greatly to reducing cracks due to hydration heat in mass concrete and high strength concrete, and improve quality.

Trend Evaluation of Self-sustaining, High-efficiency Corrosion Control Technology for Large-scale Pipelines Delivering Natural Gas by Analyzing Patent Data (특허데이터 분석을 통한 천연가스 공급용 대규모 파이프라인을 위한 자립형 고효율 부식 방지 기술의 동향평가)

  • Lee, Jong-Won;Ji, Sanghoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.730-736
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    • 2019
  • The demand for natural gas, which is considered an environmentally friendly energy source, is increasing, and at the same time, the market share of large pipelines for natural gas supply is increasing continuously. On the other hand, the corrosion of such large pipelines reduces the efficiency of natural gas transportation. Therefore, this study aims to establish a strategy for securing the patent rights of related technologies through quantitative analysis of patents on energy-independent high-efficiency corrosion prevention technology for large-scale pipelines for natural gas supply. In this patent technology trend study, Korean, US, Japanese, and European patents filed, published, and registered by June 2018 were analyzed, and a technical classification system and classification criteria were prepared through expert discussion. To use fuel cells as an external power source to prevent the corrosion of natural gas large-scale pipelines, it is believed that rights can be claimed using an energy control system and methods having 1) branch structures of pipeline and facility designs (decompressor/compressor/heat exchanger) and 2) decompression/preheating and pressurization/cooling technology of high pressure natural gas.

Formation Control of Calcium and Magnesium Compounds by Electrodeposition Process in Seawater (해수 중 전착 프로세스에 의한 칼슘 및 마그네슘 화합물의 형성 제어)

  • Park, Jun-Mu;Hwang, Seong-Hwa;Choe, In-Hye;Gang, Jun;Lee, Myeong-Hun
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.164-164
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    • 2017
  • 해양환경 중 많이 사용되는 철강재료들은 그 가혹한 부식환경에 대응하기 위하여 일반적으로 피복 도장방식법이나 음극방식법이 적용되고 있다. 여기서 음극방식법은 선박 및 해양구조물의 해중부 부식에 대해 가장 효과적인 방식법으로 알려져 있다. 한편, 이와 같이 해수 중 철강재에 음극방식을 적용할 경우, 피방식체인 그 강재 표면에 해수 중 용존된 산소의 음극환원 반응이 일어나며 국부적인 알카리 표면 조건을 형성시켜 $Mg(OH)_2$$CaCO_3$의 막을 석출시킨다. 이와같이 음극방식 중 형성된 전착물은 방식해야 될 표면적을 감소시켜 방식전류밀도를 감소시키는 효과가 있는 것으로 보고되고 있다. 이렇게 석출된 전착물은 음극표면에 부분적으로 형성되고, 여러 가지 환경 조건 등의 영향을 받아 그 피막의 형성 정도도 가늠하기 어렵기 때문에 음극방식 설계 시 그 정도에 따른 영향을 고려-반영하기가 곤란하다. 또한 이 전착물은 그 형성 메커니즘에 관한 해석이나 강도, 균일한 밀착성, 장기적인 방식효과 및 효율성 등이 아직 충분히 입증되어 있지 않은 실정에 있다. 따라서 본 연구에서는 해수 중 다양한 전착 프로세스에 의해 제작된 전착물의 기간별, 도장코팅 종류별 특성변화를 분석 및 평가하고, 전착물에 의한 희생양극 소모전류 변화 측정 분석을 통해 전착막을 균일하고 치밀하게 형성시키기 위한 최적의 조건을 찾고자 하였다. 또한 석출속도, 밀착성 및 내식특성을 향상시키기 위해 해수 중 기체를 용해시켜 제작한 막의 특성을 분석-평가하였다. 본 연구에 사용된 강 기판은 일반구조용강(KS D 3503, SS400)으로 ${\varnothing}42.7{\times}1,000mm{\times}4.0t$의 형상으로 제작하였다. 인가된 전류밀도는 1, 3 및 $5A/m^2$이고 도장 코팅 종류별 전착 석출물의 형성차이 비교 분석을 위한 실험은 선박 및 해양구조물에 많이 사용되는 Universal Epoxy 도료 2종을 선정하여 진행하였다. 또한 Steel Wire Mesh의 영향을 알아보기 위해 Mesh를 설치하여 실험을 진행하였다. 기간별-도장 종류별 외관관찰, 전착물의 두께 측정, SEM, EDS 및 XRD를 통해 막의 모폴로지, 조성원소 및 결정구조를 분석하였으며, 전착물의 내식성과 내구성을 평가하기 위해 테이핑 테스트(Taping Test) 및 전기화학적 양극분극 시험을 실시하였다. 희생양극 소모율에 대한 전착물의 영향을 확인하기 위해 외부전원을 인가하여 전착 피막을 형성시킨 강 기판에 희생양극을 연결하여 희생양극 소모효율 측정 시험을 진행하였다. 전착물의 석출량은 시간 및 전류밀도의 증가에 따라 비례하여 증가하였으며, 음극전류 인가 시 금속과 용액 계면 사이의 확산층에서 발생한 $OH^-$ 이온으로 인해 금속과 용액 계면 사이 pH가 부분적으로 증가하여 $Mg(OH)_2$ 화합물이 많이 생성되는 것으로 확인되었다. 또한 Mesh의 부착으로 평활하지 않게 형성된 미세한 굴곡구조 및 표면적 증가로 인하여 단계적으로 피복되는데 필요한 시간이 지연되면서 $CaCO_3$에 비해 $Mg(OH)_2$ 화합물이 상대적으로 증가한 것으로 사료된다. $CaCO_3$(Aragonite) 구조는 견고한 피막으로 치밀하고 화학적 친화력이 높아 우수한 밀착성을 보였으며 전착물의 영향으로 양극 전류가 감소하였고, 이로인해 방식전류 절감효과를 얻을 수 있을 것으로 기대된다.

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Detailed Deterioration Diagnosis and Analysis of Site Environment for the Taean Dongmunri Maaesamjonbulipsang (Rock-carved Standing Triad Buddha Statue), Korea (태안 동문리 마애삼존불입상의 정밀 훼손도 진단과 위치환경 분석)

  • Lee, Sun-Myung;Jun, Byung-Kyu;Shin, Eun-Jeong;Lee, Chan-Hee
    • Journal of Conservation Science
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    • v.27 no.4
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    • pp.381-393
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    • 2011
  • This study focus on the surface deterioration diagnosis based on the material characteristics of Taean Dongmunri Maaesamjonbulipsang(rock-carved standing triad Buddha Statue) and the weathering environment analysis. Rock materials of the triad Buddha is coarse-grained biotite granite which is composed mainly of quartz, K-feldspar, plagioclase, biotite and muscovite. The triad Buddha was serious surface irregularity(13.5%), exfoliation(12.3%) and discoloration(46.6%), and the physical characteristics using ultrasonic velocity appeared fourth grade (0.59, highly weathered). Korean style wooden shelter of the triad Buddha(closed type) maintained high humidity environment (daily mean; 86.6%) than exterior environment, and appeared the possibility of deterioration by freezing-thawing because the temperature showed below zero temperatures in winter. The shelter was changed from closed-type to open-type to relieve the moisture problem recently. But the moisture problem is yet to be solved because surface water flowing along northern wayside is flowed into triad Buddha. Therefore, environmental control will need to stop inflow of water into triad Buddha.