• Korean Journal of Animal Reproduction
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• v.27 no.3
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• pp.259-268
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• 2003

Lactadherin (formerly known as BA46), a major glycoprotein of the human milk fat globule membrane, is abundant in human breast milk and breast carcinoma cells and is known to prevent symptomatic rotavirus infections. In this study, we tried to transfer the human lactadherin gene to the Chinese Hamster Ovary (CHO) cells using retrovirus vector system and tested inducible expression of the gene under the tetracycline-controllable promoter. At first, tetracycline-mediated inducibility was tested using E.coli LacZ marker gene. NIH3T3 cells co-infected with RevTet-On and RevTRE-LacZ retrovirus vectors showed that the cells responded to doxycycline (a derivative of tetracycline) in a dose-dependent manner, and prominent induction of the lacZ gene expression was observed from 1 $\mu\textrm{g}$/ml of doxycycline concentration. Based on the results of the pilot experiment, inductional expression of the human lactadherin gene was conducted using RevTet-On and RevTRE-Ltd retrovirus vectors. Analysis with the RT-PCR demonstrated successful inductional expression of the lactadherin gene in the Chinese Hamster Ovary (CHO) cells. Considering that constitutive overexpression of the exogenous genes in the target cells causes serious physiological imbalance, the results obtained in this study will be very useful especially in the studies of gene therapy and transgenic animal production.

• Journal of the Korea Concrete Institute
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• v.14 no.5
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• pp.689-697
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• 2002

The dynamic loads and load-point displacements of concrete three-point bend (TPB) specimens had been measured. The average crack velocities measured with strain gages were 0.16 ㎜/sec ∼ 66 m/sec. The fracture energy for crack extension was determined from the difference of the kinetic energy for the load-point velocity and the strain energy without permanent deformation from the measure external work. For all crack velocities, there were micro-cracking for 23 ㎜ crack extension, stable cracking for 61 ㎜ crack extension at the maximum strain energy, and then unstable cracking. The unstable crack extension was arrested at 80 ㎜ crack extension except the tests of 66 m/sec crack velocity. The tests less than 13 ㎜/sec crack velocity and faster than 1.9 m/sec showed static and dynamic fracture behaviors, respectively. In spite of much difference of the load and load-point displacement relations for the crack velocities, the crack velocities of dynamic tests did not affect on fracture energy rate during the stable crack extension due to the reciprocal action of kinetic force, crack extension and strain energy. During stable crack extension, the maximum fracture resistances of the dynamic tests was 147％ larger than that of the static tests.

• Journal of Advanced Marine Engineering and Technology
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• v.41 no.1
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• pp.99-104
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• 2017

This study evaluated the heating performance of a hybrid heat pump system. The system was installed in a $100-m^2$ greenhouse to utilize surplus solar energy. A hybrid heat pump system was installed at Jocheon-ri, Jeju Island, for an empirical evaluation of the performance. The system consists of a heat storage tank and plate heat exchangers for several heat exchanges between the greenhouse and heat pump or storage tank. The system uses R410a as the working fluid and is controlled automatically by a defined set temperature of the greenhouse. This system incorporates two kinds of heat sources: outdoor air and a storage tank that collects heat from the topside of the greenhouse. The results showed that the heating capacity was 19.9 kW in the outdoor air source mode and 21.4 kW with direct heating from hot water in the thermal storage tank. These results are very similar to those of a previous study.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.1
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• pp.91-96
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• 2010

The hydraulic hose can be divided with the low pressure, the medium pressure, and the high pressure hose according to the applied pressure. The power steering system in a passenger car can be divided with the high pressure and the low pressure hose. This study deals with the life prediction for high pressure hose to be given impulse pressure which was generated in turning the car. To adjust with external and internal condition, impulse pressure and oil temperature need to be controlled with impulse test system. The result, which is only controlled with the pressure and oil temperature, adapted Calibrated Accelerated Life Test(CALT) method to predict the life of the high pressure hose and analyzed the swagging part by finite element method during the impulse test.

• Science of Emotion and Sensibility
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• v.7 no.4
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• pp.35-41
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• 2004

This study addressed the development of an automobile aroma blast device to reduce fatigue of drivers. We also analyzed the adaptation character of the aroma blast device, The aroma blast device was designed in a way that volatilized aroma is mixed with oxygen (air) and blasted to the outside, It was devised to possibly change on/off time of 2-Port Solenoid Valve using the 8051 micro processor, through which the amount of aroma can be controlled, We conducted an experiment to examine time for occurrence/elimination of adaptation to aroma in 20 college students using 4 types of natural fragrances, The subjects had an average age of 22$\pm$2 years and included both men and women. The results showed that the occurrence and elimination times for adaptation to aroma were 1 minute 28 seconds and 3 minutes and 15 seconds for Jasmine 30%, 2 minutes and 41 seconds and 4 minutes and 3 seconds for Jasmine 50%, 1 minute and 47 seconds and 2 minutes and 59 seconds for Peppermint 30%, and 1 minute 59 seconds and 4 minutes and 11 seconds for Peppermint 50%, respectively.

• Archives of design research
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• v.17 no.2
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• pp.351-362
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• 2004

Generally, they respond to a phenomenon, with their any way, which they have looked at in their surroundings, and also put into their action successively and variously according as what it is. A person who has not so much problems in experiential education added to mental and physical ability prefers controlling his manner by himself as seeing, listening and feeling to being cured it by other physical support. Meanwhile, even though there are tools that we use conveniently in everyday life, it is sometimes required that user is able to control his action by himself with a certain interactive function to deal with a accidental situation. For example, in the home, when they were cooking, washing dishes and taking a bath they would not often control their minds on how to act about flowing water through the faucet going back and forth between saving and easygoing. By reasons of those statements, the project has been studied to propose the new conceptual faucet which digital technology is applied to, for recognizing the volume of water flowed through water pipe as counting it with built-in flow meter, and then saving water as controling the water-flow with faucet lever. It means that homemakers can observe the flow rate of water from the faucet placed in front of the sink in kitchen and control it right away for saving water. For studying this project, the kinds and features of the various flow-meters that measure the volume of water-flow were researched and analyzed for taking a reasonable type to the new ideal faucet. According to this analyzing, turbine-flow-meter was selected as appropriate form for the digital display-built-in faucet that would be presented in this project. As the next step, the basic structure was created for developing a new conceptual faucet. Finally two models have been presented through several steps for making the suitable shape to the new style faucet.

• Journal of the Korean Geotechnical Society
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• v.17 no.6
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• pp.47-51
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• 2001

There are two strategies to cope with the troubles in landfill site after closure. The first method is active in a way that the wastes are dug up and the recyclable materials are reutilized, meanwhile the materials not recyclable are incinerated in order to minimize the volume of residues to be disposed of. The second method is rather passive and defensive in a way that the source of contamination, that is, buried wastes are not treated. Instead, the transport of leaking leachate and gases generated from the wastes are intercepted and controlled. In this study, as a passive way of the efficient leachate blocking process, applicabilities of geocrete and soluble sodium silicate as a substitute to control leachate leaking from landfill sidewall were investigated. In case of compression test, the strength of mixture I (Geocrete:Sodium silicate=1:3.9 v/v) and mixture II (Geocrete:Sodium silicate=1:2.5 v/v), even after 7 days' curing was higher than the minimum allowance to tolerate the loading(5 kg/$\textrm{cm}^2$). Soaking in the acid fur 4 days and 7 days respectively, the compressive strength of the specimens reduced seriously. The toxicity of geocrete is not detected through the bioassay test, once it was mixed with sodium silicate and the complex was formed. The hydraulic conductivity of the mixtures even after 7 days' curing was lower than the threshold limit $(1.0\times10_{-7}cm/s)$.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.6
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• pp.863-871
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• 2013

Recently application of open protocols and external network linkage to the national critical infrastructure has been growing with the development of information and communication technologies. This trend could mean that the national critical infrastructure is exposed to cyber attacks and can be seriously jeopardized when it gets remotely operated or controlled by viruses, crackers, or cyber terrorists. In this paper virtual Honeynet model which can reduce installation and operation resource problems of Honeynet system is proposed. It maintains the merits of Honeynet system and adapts the virtualization technology. Also, virtual Honeynet model that can minimize operating cost is proposed with data analysis and collecting technique based on the verification of attack intention and focus-oriented analysis technique. With the proposed model, new type of attack detection system based on virtual Honeynet, that is Cybertrap, is designed and implemented with the host and data collecting technique based on the verification of attack intention and the network attack pattern visualization technique. To test proposed system we establish test-bed and evaluate the functionality and performance through series of experiments.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.19-26
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• 2019

To construct buildings on limited land, the size of the building is important. The development process needs to be minimized because determining the size of a structurally safe building at the planning stage incurs considerable time and cost. This study proposes the Limit Slenderness Ratio Approximation Equation. This study examined an outrigger structure system among several systems proposed for controlling the lateral displacement in tall buildings. This study compared the Limit Slenderness Ratio Approximation Equation with the approximate equation by changing the variables of the building model, and examined the size of the building using the approximate Equation. As an analysis program, the MAIDAS architectural structural analysis program was used to conduct model-specific analysis. The appropriate scale of the building to minimize the error between the approximate value calculated by the Limit Slenderness Ratio Approximation Equation and the analysis result of the structural analysis program is as follows. As the number of outrigger installation increases, the error can be reduced; the ratio of the cores is reasonable, from 20% to 30%, and the arrangement of the column is suitable only for the outer column without an internal column.

• Journal of the Korean Institute of Landscape Architecture
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• v.37 no.2
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• pp.78-89
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• 2009

The purpose of this study is to build fundamental data for the classification of landscape type as a base for landscape planning and management practices. To do this, prior dissertations and landscape plan reports were analyzed, which presented the classification criteria for landscape type. Based on this, classification criteria for landscape type which could be usable in zoning ordinances has been suggested. The result is as follows: Firstly, in landscape analysis and assessment study based on ecological and formal aesthetic models, landscape type is classified by the character of the landscape element. Secondly, there is no logical classification of landscapetype in urban landscape planning according to mixed use of landscape type for analysis and planning. It is therefore difficult to identify the object of landscape planning, which is intimately linked with the shortage of concrete practice for landscape management. In connection with this issue, classification criteria for landscape type are suggested based on utility in landscape planning. This could be divided into internal criteria and external criteria. The former are land-use, topographical characteristics, characteristics of the view object, and landscape elements while the latter are viewpoint, distance to view object, and urban form. Applying the landscape type classified by the criteria suggested in this paper, it is possible to manage an entire urban area. In addition, landscape type could be reference data for operating a zoning system.