• Applied Biological Chemistry
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• v.47 no.4
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• pp.390-395
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• 2004

In order to characterize ion channels present in tomato roots, microsomes were incorporated into an artificial lipid bilayer arranged for electrophysiological analysis. Of the five different ion channels that could be found, a channel of 450 pS conductance was found most frequently. This channel displayed subconductance states of 450, 257 and 105 pS. All subconductance states showed linear current-voltage relationships. At positive holding potentials, high frequency of transient channel openings was observed; however, at negative potentials, the open times were long and open probability high. Po was 0.83 at -40 mV. When an additional 50 mM $K^+\;or\;Na^+$ was added to the cis side of bilayer, the reversal potentials shifted in the negative direction to near -10 mV. Thus, the 450 pS cation channel selects poorly between $K^+\;and\;Na^+$. In the presence of $100\;{\mu}M$ metal ions, the channel activity was severely inhibited by $La^{3+},\;Ba^{2+},\;and\;Zn^{2+}$, and Po was decreased to 0.2 or even less. However, $Al^{3+}\;and\;Cd^{2+}$ decreased the activity by only 20%. Interestingly, each metal ion showed different kinetics of channel inhibition. While $500\;{\mu}M\;La^{3+}$ inhibited the activities of all subconductance state, 1 mM $Zn^{2+}$ inhibited all except the 105 pS state. $Cd^{2+}$ changed the gating of the channel from a long-opening state to brief transient openings even at negative holding potentials. These data represent that the metal ions may have different binding sites on the channel protein and could be useful modulators and probes to investigate structural characteristics as well as the functional roles of the 450 pS channel on the root physiology.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.38 no.4
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• pp.284-288
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• 2002

The authors examined the response of electrocardiogram (ECG) to Mirror carp, Cyprinus carpio of body length. The experiments were performed of three classes (10~15, 15~20 and 20~25cm) were given to fishes with an electrode inserted into their bodies and then their ECGs were recorded continuously for 30 minutes in 16-18$^{\circ}C$. The results which are divided into by day and by night and then analyzed by fishes' conditions are as follows; 1 In case of body length 10~15 cm, the average heart rate 43.4 beat/min by day and 45.9 beat/min by night, the average action potential 4.38 $mutextrm{V}$ by day and 3.64 $mutextrm{V}$ by night, in narcotism condition(0~9 min). the average heart rate 69.4 beat/min by day and 67.4 beat/min by night, the average action potential 3.82 $mutextrm{V}$ by day and 3.50 $mutextrm{V}$ by night, in stable condition(9~30 min). 2. In case of body length 15～20 cm, the average heart rate 42.2 beat/min by day and 45.4 beat/min by night, the average action potential 4.13 $mutextrm{V}$ by day and 3.95 $mutextrm{V}$ by night, in narcotism condition(0-5 min). the average heart rate 67.6 beat/min by day and 65.3 beat/min by night, the average action potential 4.58$mutextrm{V}$ by day and 4.61 $mutextrm{V}$ by night, in stable condition (5~30 min). 3. In case of body length 20～25 cm, the average heart rate 47.5 beat/min by day and 47.5 beat/min by night, the average action potential 4.81 $mutextrm{V}$ by day and 4.20 $mutextrm{V}$ by night, in narcotism condition(0-4 min). the average heart rate 67.5 bea/min by day and 64.8 beat/min by night, the average action potential 5.31 $mutextrm{V}$ by day and 4.90 $mutextrm{V}$ by night, in stable condition (4~30 min).

• Journal of Life Science
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• v.20 no.11
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• pp.1711-1717
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• 2010

In this study, fibrinolytic activities of fermented yellow agabean (FYA) and black agabean (FBA), and the antioxidation efficiencies of 70% ethanol extract of fermented yellow agabean (FYAE) and black agabean (FBAE) were investigated by selecting Bacillus sp. sm26 strain. Fibrinolytic activities of FYA and FBA were $6.38{\pm}0.5$ and $6.83{\pm}0.5\;U/ml$, which were 1.3 and 1.4 times higher than that of FSB, respectively. With regard to total phenolic contents, FYAE and FBAE were $3.40{\pm}0.44\;mg/g$ and $2.45{\pm}0.20\;mg/g$ respectively, suggesting that their contents were about twice as high as that of fermented soybean extract (FSBE) used as a control. In comparison with FSBE, total protein and sugar contents of FYAE were $0.56{\pm}0.11$ and $2.41{\pm}0.48\;mg/g$, respectively, and those of FBAE were $0.39{\pm}0.12$ and $2.72{\pm}0.63\;mg/g$, respectively. This result suggests that FYAE was 4.7 and 1.7 times higher than FSBE, respectively. The DPPH radical scavenging activity of FBAE was 79% at 1 mg/ml, which was highest among the fermented bean extracts, and was twice as high as FSBE in regards to activity. In addition, FBAE exhibited the highest reducing power at 1 mg/ml, which was higher than FSBE by two-fold. With regard to lipid peroxidation, FBAE and FYAE were 93% and 80% at 1 mg/ml, which were 3 and 2.5 times higher than FSBE, respectively. Of note, the hydrogen peroxide scavenging activities of FBAE and FYAE were 82% and 54% at 1 mg/ml, offering activity that was 4 and 2.5 times higher than FSBE, respectively. Based on these results, the fibrinolytic activity and antioxidation efficiency of the fermented agabeans were significantly higher than other soybeans. Therefore, these studies may suggest that the functional agabeans can be a potential candidate for a natural functional food.

• Journal of Life Science
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• v.23 no.1
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• pp.63-68
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• 2013

The effect of black garlic extract on the activation of spleen cells from a C57BL6 mouse was investigated to examine immune activities of of fermented black garlic containing a variety of bioactive substances. xtract obtained from the concentration of commercial Namhae black garlic was used for the analysis of immune activities. Treatment with the extract increased the expression of interleukin-2 (IL-2) cytokine. The simultaneous administration of the extract plus lipopolysaccharide (LPS) increased the expression of IL-2, tumor necrosis factor (TNF)-${\alpha}$, and interferon (IFN)-${\gamma}$ compared with that of a control group. This result suggests that cellular immunity can be induced by macrophages, resulting in the expression of T lymphocytes and T helper type 1 (Th1) cells. In addition, treatment with the extract increased the late response of IL-6 cytokines, and the extract plus LPS augmented the expression of IL-4 and IL-6 compared with that of an LPS-treated group. Meanwhile, the extract plus LPS decreased the late response of IL-10, suggesting that humoral immunity can be activated by stimulating B lymphocytes, suppressing cellular immunity, and effectively modulating the conversion into humoral immune responses. These findings demonstrate that the black garlic extract activates Th1 and Th2 cells by stimulating T lymphocytes in mouse spleen cells and leads to immunomodulation by activating cellular and humoral immune responses of the immune system.

• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.203-213
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• 1985

The in situ posture of anterior body of Metagonimus yokogawai was observed in experimental metagonimiasis of dog. The metacercariae were collected from naturally infected sweetfish by peptic digestion; a total of 8 dogs was orally infected with 10,000 metacercariae respectively. Two dogs were killed on 3 days, 9 days, 4 weeks and 10 weeks after the infection. The postures of worms in histological section of small intestine and of whole worms collected from the fixed intestinal mucosa were examined by light and scanning electron microscope. The results were summarized as follows: 1. The recovery rates of worms were 42.6% on 3 days, 55.0% on 9 days, 33.2% on 4 weeks and 9. 8% on 10 weeks after the infection respectively. 2. In histological sections of small intestine, most of worms were found at intervillous spaces as ovoid sections of posterior body. However, many worms, especially in 3 day-old worms, revealed protruded anterior body in glandular lumens of crypt. Some sections of anterior body were bifurcated or sacculated. 3. The worms collected from fixed intestinal mucosa under dissecting microscope exhibited a variety of postures. Many worms showed flat shapes with a concavity or curvatures. However, in many worms, the anterior body made a single or multiple protrusions. 4. By SEM observation of protruded anterior body, a longitudinal groove was found. 5. The frequency of worms with protruded anterior body decreased in 9 days, 4 weeks and 10 weeks than in 3 day-old worms. The above findings indicated that the anterior body of juvenile and adult M. yokogawai protruded to lumens of glandular crypt by folding their lateral portions to make a reversible tubelike structure. Frequent multiple protrusions were considered to be made to adapt the microniche of glandular crypts of dog intestine.

• Progress in Medical Physics
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• v.12 no.2
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• pp.161-169
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• 2001

For the head and neck radiotherapy, the technique of half beam using independent collimator is very useful to avoid overlapping of fields particularly when the lateral neck fields are placed adjacent to anterior supraclavicular field. Also abutting photon field with electron field is frequently used for the irradiation of posterior neck when tolerable dose on spinal cord has been reached. Using 6 MV X-ray and 9 MeV electron beams of Clinac1800(Varian, USA) linear accelerator, we performed film dosimetry by the X-OMAT V film of Kodak in solid water phantom and the dose distribution at beam center of 2 half beams further examined according to depths(0 cm, 1.5 cm, 3 cm, 5 cm) for single anterior half beam and anterior/posterior half beam. The dose distribution to the junction line between photon and electron fields was also measured. For the single anterior half beam, the absorption doses at 0.3 cm, 0.5 cm and 1 cm distances from beam center were 88%, 93% and 95% of open beam, respectively. In the anterior/posterior half beams, the absorption doses at 0.3 cm, 0.5 cm and 1 cm distances from beam center were 92%, 93% and 95% of open beam, respectively At the junction line between photon and electron fields, hot spot was developed on the side of the photon field and a cold spot was developed on that of the electron field. The hot spot in the photon side was developed at depth 1.5 cm with 7 mm width. The maximum dose of hot spot was increased to 6% of reference doses in the photon field. The cold spot in the electron side was developed at all measured depths(0.5 cm-3 cm) with 1-12.5 mm widths. The decreased dose in the cold spot was 4.5-30% of reference dose in the electron field. With above results, we concluded that when using electron beam or independent jaw for head and neck radiotherapy, the hot and cold dose area should be considered as critical point.

• Radiation Oncology Journal
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• v.23 no.1
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• pp.51-60
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• 2005

Purpose : Selective inhibition of multiple molecular targets may improve the antitumor activity of radiation. Two specific inhibitors of selective cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) were combined with radiation on the HeLa cell line. To investigate cooperative mechanism with selective COX-2 inhibitor and EGFR blocker, in vitro experiments were done. Materials and Methods : Antitumor effect was obtained by growth inhibition and apoptosis analysis by annexin V-Flous method. Radiation modulation effects were determined by the clonogenic cell survival assay. Surviving fractions at 2 Gy ($SF_2$) and dose enhancement ratio at a surviving fraction of 0.25 were evaluated. To investigate the mechanism of the modulation of radiosensitivity, the cell cycle analyses were done by flow cytometry. The bcl-2 and bax expressions were analyzed by western blot. Results : A cooperative effect were observed on the apoptosis of the HeLa ceil line when combination of the two drugs, AG 1478 and NS 398 with radiation at the lowest doses, apoptosis of $22.70\%$ compare with combination of the one drug with radiation, apoptosis of $8.49\%$. In cell cycle analysis, accumulation of cell on $G_0/G_l$ phase and decrement of S phase fraction was observed from 24 hours to 72 hours after treatment with radiation, AG 1478 and NS 398. The combination of NS 398 and AG 1478 enhanced radiosensitivity on a concentration-dependent manner in HeLa cells with dose enhancement ratios of 3.00 and $SF_2$ of 0.12 but the combination of one drug with radiation was not enhanced radlosensitivity with dose enhancement ratios of 1.12 and SF2 of 0.68 (p=0.005). The expression levels of bcl-2 and bax were reduced when combined with AG 1478 and NS 398. Conclusion : Our results indicate that the selective COX-2 inhibitor and EGFR blocker combined with radiation have potential additive or cooperative effects on radiation treatment and may act through various mechanisms including direct inhibition of tumor cell proliferation, suppression of tumor cell cycle progression and inhibition of anti-apoptotic proteins.

• Korea journal of population studies
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• v.20 no.1
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• pp.129-159
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• 1997

The main purpose of this study is to describe the different life chances among various occupational groups in Chonbuk Province which has been excluded in the process of Korea's industrialization since the 1960s. More specifically, this study analyzed to what extent 6 occupational groups have the different life chances in the sub-levels such as health conditions and leisure life. According to our data analysis, it is found that the 6 occupational groups have the different life chances. That is, while those who are engaged in the higher prestigious occupation have a good life chance, those who in the lower prestigious occupation have a worse life chance. For example, among 6 occupational groups, professional-managerial who secure the higher autonomy and stability in their work have the highest life chance in the labor and health, and leisure life. Next, professional-technical, white-collar, sales-service occupants have the higher life chance after the professional-managerial. However, these 3 occupation groups are partially inconsistent in the two sectors of the life chance. Specifically, the professional-technical, despite their autonomy and stability in the work world, conceived that their work conditions are worse as well as badly influencing on their own health. On the other band, the sales-service workers group, despite their lower social status, is relatively autonomous and stable in their work environment and thus has the higher life chances. Finally, agricultural and productive workers have relatively the lower lift chances. Nevertheless, there are some clear differences among these 2 occupational groups. That is, the farmers have relatively the higher life chances in the sectors of socio-economic characteristics, and labor and health rather than productive workers. As a result, the productive workers are generally located in the lowest level of life chance. This fact is particularly attributed to the fragility of manufacturing industries in Chonbuk Province.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.528-533
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• 2009

In the current study, the mesenchymal stem cells (MSCs) isolated and propagated from the human umbilical cord blood (UCB) were tested for their capabilities of differentiation into chondrocytes in vitro. The mesenchymal progenitor cells (MPCs) collected from UCB were cultured in a low glucose DMEM medium with 10% FBS, L-glutamine and antibiotics. The human MSC colonies were positively stained by PAS reaction. When the immunophenotypes of surface antigens on the MSCs were analyzed by fluorescence-activated cell sorter (FACS) analysis, these cells expressed positively MSC-related antigens of CD 29, CD44, CD 90 and CD105, whereas they did not express antigens of CD14, CD31, CD34, CD45, CD133 and HLA-DR. Following induction these MSCs into chondrocytes in the chondrogenic differentiation medium for 3 weeks or more, the cells were stained positively with safranin O. We clearly confirmed that human MSCs were successfully differentiated into chondrocytes by RT-PCR and immunofluorescent stain of type-II collagen protein. These data also indicate that the isolation, proliferation and differentiation of the hUCB-derived MSCs in vitro can be used for elucidating the mechanisms involved in chondrogenesis. Moreover this differentiation technique can be applied to developing cell-based tissue regeneration or repair damaged tissues.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.50-56
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• 2002

This study was carried out to optimize the fertigation method using fermented swine liquid manure for the growth of two western vegetables, broccoli and celery. Plants were grown in a rain-shelter house and fertilized with a range of dilutions(efflux 5 dilution=Ef. 5, efflux 10 dilution=Ef. 10, efflux 25 dilution=Ef. 25, and efflux 50 dilution=Ef. 50) of the liquid manure or with conventional application of N : P$_2$O$\_$5/ : K$_2$O = 200 : 70 : 500kg/ha for broccoli, 250 : 210 : 240 kg/ha for celery as controls. After harvest, soil pH and K content decreased after using a high concentration of the liquid manure, Ef. 5, than after treatment with weaker concentrations at Ef. 25 and Ef. 50. On the other hand, soil electrical conductivity, content of P$_2$O$\_$5/, organic matter, total nitrogen, and NO$_3$-N at Ef. 5 increased as concentration of swine liquid manure increased. After harvest, available P$_2$O$\_$5/ in plant tissue did not differ significantly between any of the treatments. In broccoli, the lower concentration (Ef. 50) of swine liquid manure increased flowering over the other treatments, perhaps because the level of absorption into the plants is higher with lower concentration. The amounts of K and Ca in plant tissue were greatest after Ef. 25 and Ef. 50 treatments. Plant growth was best at Ef. 50 in broccoli, head height, head width, and head weight were the best with Ef. 25 and Ef. 50 treatments after harvest. In celery, leaf length was greater after Ef. 25 and Ef. 50 treatments than any other treatments. Total yield of celery of Ef. 25 and Ef. 50 treatments was twice that of conventional cultivation. On the other hand, yield severely decreased after application of high-concentration treatment at Ef. 5. In conclusion, fertigation of swine liquid manure, diluted in the range of Ef. 25 to Ef. 50, could improve yield and quality in broccoli and celery.