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http://dx.doi.org/10.5352/JLS.2013.23.1.63

Effect of Black Garlic Extract on Cytokine Generation of Mouse Spleen Cells  

Seo, Min Jeong (Medi-Farm Industrialization Research Center, Dong-A University)
Kang, Byoung Won (Medi-Farm Industrialization Research Center, Dong-A University)
Park, Jeong Uck (Medi-Farm Industrialization Research Center, Dong-A University)
Kim, Min Jeong (Department of Biotechnology, Dong-A University)
Lee, Hye Hyeon (Department of Biotechnology, Dong-A University)
Ryu, En Ju (Department of Cosmetology, Hanseo University)
Joo, Woo Hong (Department of Biology, Changwon National University)
Kim, Kwang Hyuk (Department of Microbiology, Kosin University College of Medicine)
Jeong, Yong Kee (Medi-Farm Industrialization Research Center, Dong-A University)
Publication Information
Journal of Life Science / v.23, no.1, 2013 , pp. 63-68 More about this Journal
Abstract
The effect of black garlic extract on the activation of spleen cells from a C57BL6 mouse was investigated to examine immune activities of of fermented black garlic containing a variety of bioactive substances. xtract obtained from the concentration of commercial Namhae black garlic was used for the analysis of immune activities. Treatment with the extract increased the expression of interleukin-2 (IL-2) cytokine. The simultaneous administration of the extract plus lipopolysaccharide (LPS) increased the expression of IL-2, tumor necrosis factor (TNF)-${\alpha}$, and interferon (IFN)-${\gamma}$ compared with that of a control group. This result suggests that cellular immunity can be induced by macrophages, resulting in the expression of T lymphocytes and T helper type 1 (Th1) cells. In addition, treatment with the extract increased the late response of IL-6 cytokines, and the extract plus LPS augmented the expression of IL-4 and IL-6 compared with that of an LPS-treated group. Meanwhile, the extract plus LPS decreased the late response of IL-10, suggesting that humoral immunity can be activated by stimulating B lymphocytes, suppressing cellular immunity, and effectively modulating the conversion into humoral immune responses. These findings demonstrate that the black garlic extract activates Th1 and Th2 cells by stimulating T lymphocytes in mouse spleen cells and leads to immunomodulation by activating cellular and humoral immune responses of the immune system.
Keywords
Black garlic extract; cytokine; T lymphocytes;
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