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Identification of specific SNP molecular marker from Cudrania tricuspidata using DNA sequences of chloroplast TrnL-F region (구지뽕 나무의 엽록체 TrnL-F 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 확인)

  • Lee, Soo Jin;Shin, Yong-Wook;Kim, Yun-Hee;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.44 no.2
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    • pp.135-141
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    • 2017
  • Cudrania tricuspidata Bureau is a widely used medicinal perennial woody plant. For conservation and germplasm utilization of the plant, it is imperative to obtaining information regarding the genetic diversity of the plant populations. Although C. tricuspidata is an important medicinal plant registered in South Korea, no molecular markers are currently available to distinguish Korean-specific ecotypes from other ecotypes of different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific ecotypes of C. tricuspidata via the amplification refractory mutation system (ARMS)-PCR analyses. Molecular authentication of twelve C. tricuspidata ecotypes from different regions was performed, using DNA sequences in the trnL-F chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific C. tricuspidata ecotypes from different regions.

Assessment of Genetic Relationship among Watermelon Varieties Revealed by ISSR Marker (Inter-simple sequence repeat (ISSR) marker를 이용한 수박의 품종간 유연관계 분석)

  • Kwon Yong-Sham;Lee Won-Sik;Cho Il-Ho
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.219-224
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    • 2006
  • Inter-simple sequence repeat (ISSR) analysis were used to assess genetic diversity among 18 genotypes of watermelon (Citrullus lanatus Thunb.) including breeding lines and commercial varieties. The 21 ISSR primers selected from 100 primers were showed the amplification of 105 reproducible fragments ranging from about 200 bp to 5000 bp. A total of 58 DNA fragments were polymorphic with an average 2.7 polymorphic bands per primer. The polymorphic primers were divided into 18 anchored primers and 3 non anchored primers. All of the anchored primers were di-nucleotide repeat motif, and was more polymorphic than non anchored primers. Eighteen watermelon genotypes were classified into two large groups. Clustering was in some accordance with the division of fruit shape into 18 watermelon. Therefore, ISSR markers may be suitable for variety discrimination and for constructing a linkage map of watermelon.

Putative Bax inhibitor from rice a conserved cell death suppressor, is isolated by yeast functional screening (효모 기능 선발을 이용한 벼의 세포사유발을 억제하는 유전자 선발)

  • Lee, Gyu Ho;Son, Ye Jin;Sawitri, Widhi Diya;Sohn, Jae-Keu;Kim, Kyung-Min
    • Current Research on Agriculture and Life Sciences
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    • v.29
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    • pp.37-42
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    • 2011
  • The plant-homologue of Bax Inhibitor, a gene described to suppress the cell death induced by Bax gene expression in yeast, was isolated from rice (Oryza sativa L.). Nucleic acid sequence and amino acid sequence were 741 bp and 247 bp, respectively. The amino acid sequence of the predicted protein was well conserved in plant (84 % in amino acids) and contained five membrane-spanning segments.

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Development of specific SNP molecular marker from Thistle using DNA sequences of ITS region (엉겅퀴의 ITS 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.45 no.2
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    • pp.102-109
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    • 2018
  • Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

Isolation and Characterization of Saccharomyces cerevisiae from nuruk for Production of Ethanol from Maltose (누룩으로부터 맥아당 이용능과 에탄올 생산성이 우수한 효모의 분리와 특성)

  • Choi, Da-Hye;Choi, Yeong-Hwan;Yeo, Soo-Hwan;Kim, Myoung-Dong
    • Microbiology and Biotechnology Letters
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    • v.44 no.1
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    • pp.34-39
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    • 2016
  • Wild-type yeast strains were isolated from nuruk, a type of microbial starter culture used for fermenting grains to produce alcoholic products, that was collected from different areas in Korea. Strains were identified based on the analysis of 18S rRNA sequences. Fifty strains shared the highest sequence similarity with Saccharomyces cerevisiae and were designated MBYK1-MBYK50. Among these S. cerevisiae isolates, MBYK45 produced $44.0{\pm}0.3g$ of ethanol from 200 g maltose after incubation at $30^{\circ}C$ for 48 h. Maximum ethanol production of $110.80{\pm}0.81g/l$ with productivity of $3.79{\pm}0.14g^{-1}l^{-1}h^{-1}$ was obtained at optimum culture conditions of pH (6.0), maltose (200 g/l), and temperature ($35^{\circ}C$). This study indicates that the MBYK45 strain of S. cerevisiae, isolated from nuruk, might be suitable for traditional liquor production from malts.

Identification of the Cell-envelope Proteinase of Lactic Acid Bacteria Isolated from Kimchi. (김치 유래 젖산균의 Cell-envelope Proteinase 존재 확인)

  • 이유진;최재연;이형주;장해춘;김정환;정대균;김영석;김소미;이종훈
    • Microbiology and Biotechnology Letters
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    • v.30 no.2
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    • pp.116-122
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    • 2002
  • The partial 165 rDNA sequences of 6 lactic acid bacterial strains isolated from Kimchi were determined. Two strains were Leuconostoc mesenteroides and the rest were incorrectly classified and turned out to be Lactobacillus. As the case of dairy lactic acid bacteria, the strains isolated from Kimchi also had cell-envelope proteinase (CEP) activity. As the result of partial CEP gene amplification with CEP-specific primers, the expected 1.2-kb amplificate was obtained not from Leu. mesenteroides but from Lactobacillus strains. The deduced amino acid sequence of PCR product amplified from the genomic DNA of Lactobacillus pentosus KFR1821 showed 95% and 92% homology with those of PrtPs from Lactococcus lactis subsp. cremoris and Lactobacillus paracasei subsp. paracasei, respectively. The PCR amplificate was used as a probe and the result of Southern hybridization illuminated the location of CEP gene in chromosomal DNA of Lb. pentosus KFR1821.

Study on the Genetic Variation of the Mitochondrial DNA in the Beet Armyworm, Spodoptera exigua (H bner), Using PCR-RFLP (PCR-RFLP를 이용한 파방나방 (Spodoptera exigua(H bner)) 미토콘트리아 DNA의 유전변이 연구)

  • 김용균;이명렬;정충렬
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.23-30
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    • 1998
  • Restriction fragment length polymorphism (RFLP) of a DNA has been a useful tool for analyzing genetic variation. This research was performed to establish an RFLP analytic method on the mitochondrial DNA (mtDNA) of the beet armyworm, Spodoptera exigua (Hiibner). To do this, total size of the mtDNA was measured and polymerase chain reaction (PCR) primers were selected. Its mitochondrial genome size was ca. 16kb. From a serial PCR test of 29 primers refered to the compilation of Simon et al. (1994), 22 primers were selected to amplify its mtDNA fragments. These primers resulted in short (300-700 bp) or long (1000-2000 bp) DNA products which represented a total or partial sequence of each of CO-I, CO-11, Cyt-B, ND-1, 12s rRNA, 16s rRNA, and some tRNAs. PCR-RFLP was performed in some variable mtDNA regions with 8 kinds of 4bp recognizing restriction enzymes. Different populations from Andong, Kyungsan, and Sunchun did not show any restriction site polymorphisms but had some length variation in certain regions of mtDNA.

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Proteome Analysis of Drosophila melanogaster Used 2-DE and MALDI- TOF-MS (이차원 전기영동과 펩타이드 지문 검색법을 이용한 초파리의 프로테옴 분석)

  • Park Jeong-Won;Cha Jae-Young;Song Jae-Young;Kim Hee-Kyu;Kim Beom-Kyu;Jeon Beong-Sam
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.427-433
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    • 2005
  • With the completely discovery of the Drosophila genome sequence, the next great challenge is to extract its biological information by systematic expression and to perform functional analysis of the gene. Here we reported a proteome analysis of D. melanogaster with two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The cell extracts of D. melanogaster, $200{\mu}g$ were resolved to more than 400 silver-stained spots by 2-DE. The most abundant protein spots were ranged from 4.0-7.5 of pI and from 15-90 kDa of molecular weight. The excised spots were destained and in-gel digested by trypsin. The masses of the resulting peptide mixtures were measured by MALDI-TOF-MS. Identified proteins were compared with measured peptide mass and a dynamic peptide searching database which is accessible via the internet. The results revealed that identified proteins were produced by 59 genes derived from 65 protein spots.

Molecular Cloning of the Gene for $\alpha$-Acylamino-$\beta$-lactam Acylhydrolase from Acetobacter turbidans by Immunochemical Detection Method (면역화학적 방법에 의한 Acetobacter turbidans의 $\alpha$-Acylamino-$\beta$-lactam Acylhydrolase의 유전자 클론화)

  • Nam, Doo-Hyun;Dewey D.Y. Ryu
    • Microbiology and Biotechnology Letters
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    • v.16 no.5
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    • pp.363-368
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    • 1988
  • Molecular cloning of gene for $\alpha$-acylamino-$\beta$-lactam acylhydrolase (ALAH) III from Acetobacter turbidans has been attempted by immunochemical detection method, in which polyclonal antibody from mouse Balb/c against this enzyme was employed as a probe. As a cloning vector, λ gtll was chosen for this purpose. Two positive clones has been selected from genomic libraries of A. turbidans, which had somewhat different binding affinities on anti-ALAH III umm and anti-$\beta$-galactosidase. By restriction analysis, both clones has been turned out to lose one of EeoRI sites. From these results, it concluded that deletion of DNA between lacZ gene and inserted DNA has occurred during replication of these clones in host cells.

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Molecular Biological Characteristics of Vibrio cholerae O1 Isolated from Diarrheal patients in the Gyeongbuk province. (최근 경북지역 설사환자 검체에서 분리된 Vibrio cholerae O1의 분자생물학적 특성)

  • 이상조;이복권;이건주;이희무
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.334-341
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    • 2003
  • This study was carried out to investigate the cause of cholera outbreak in Gyeongbuk province in 2001.90 strains of Vibrio cholerae O1 El Tor serotype Inaba were isolated from diarrheal patients. By multiplex-PCR, all of the isolated strains revealed positive for detection ctxA, hlyA and tcpA genes. There were DNA sequence difference of the cholera-toxin subunit A gene and subunit B gene between isolated V. cholerae O1 and the strain of GenBank. In analysis of PFGE patterns, all of the isolated strains were showed the same DNA fragments. We also collected plankton samples in the east coast of Gyeongbuk to isolate V. cholerae O1 and V. cholerae O139 from August to October 2002. The samples were examined to detect the rfb gene and cholera-toxin gene by multiplex-PCR. The cholera-toxin gene was detected and then we tried to isolate V. cholerae O1 and V. cholerae O139, but they were not isolated.