Current Research on Agriculture and Life Sciences
- Volume 29
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- Pages.37-42
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- 2011
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- 2287-271X(pISSN)
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- 2288-0356(eISSN)
Putative Bax inhibitor from rice a conserved cell death suppressor, is isolated by yeast functional screening
효모 기능 선발을 이용한 벼의 세포사유발을 억제하는 유전자 선발
-
Lee, Gyu Ho
(Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture & Life Science, Kyungpook National University)
;
- Son, Ye Jin (Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture & Life Science, Kyungpook National University) ;
- Sawitri, Widhi Diya (Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture & Life Science, Kyungpook National University) ;
-
Sohn, Jae-Keu
(Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture & Life Science, Kyungpook National University)
;
-
Kim, Kyung-Min
(Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture & Life Science, Kyungpook National University)
-
이규호
(경북대학교 농업생명과학)
;
- 손예진 (경북대학교 농업생명과학) ;
- 위디 (경북대학교 농업생명과학) ;
-
손재근
(경북대학교 농업생명과학)
;
-
김경민
(경북대학교 농업생명과학)
- Received : 2011.11.30
- Accepted : 2011.12.20
- Published : 2011.12.31
Abstract
The plant-homologue of Bax Inhibitor, a gene described to suppress the cell death induced by Bax gene expression in yeast, was isolated from rice (Oryza sativa L.). Nucleic acid sequence and amino acid sequence were 741 bp and 247 bp, respectively. The amino acid sequence of the predicted protein was well conserved in plant (84 % in amino acids) and contained five membrane-spanning segments.
본 연구에서는 효모에서 과 발현하는 Bax inhibior와 관련된 유전자를 동정하여 특성화 하였다. Yeast functional screening이라는 방법을 이용하여, 일반적은 환경에서 재배된 벼의 cDNA를 QX95001에 형질전 환하여 SD-galactose-Leu--Ura-배지에서 생성된 8개의 클론을 선발하였다. 그 중 AtBI-1과 같은 domain이 있는 D2-234를 포함하여 5개의 클론을 선발하였다. D2-243는 741bp의 염기서열과 247개의 아미노산으로 구성되었고 5 membrane-spanning 단편으로 되어 있음을 확인하였다. D2-234는 SD-galactose-