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Molecular Biological Characteristics of Vibrio cholerae O1 Isolated from Diarrheal patients in the Gyeongbuk province.  

이상조 (경북 보건환경연구원 미생물과)
이복권 (국립보건원 세균질환부 장내세균과)
이건주 (안동대학교 생물학과)
이희무 (안동대학교 생물학과)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.4, 2003 , pp. 334-341 More about this Journal
Abstract
This study was carried out to investigate the cause of cholera outbreak in Gyeongbuk province in 2001.90 strains of Vibrio cholerae O1 El Tor serotype Inaba were isolated from diarrheal patients. By multiplex-PCR, all of the isolated strains revealed positive for detection ctxA, hlyA and tcpA genes. There were DNA sequence difference of the cholera-toxin subunit A gene and subunit B gene between isolated V. cholerae O1 and the strain of GenBank. In analysis of PFGE patterns, all of the isolated strains were showed the same DNA fragments. We also collected plankton samples in the east coast of Gyeongbuk to isolate V. cholerae O1 and V. cholerae O139 from August to October 2002. The samples were examined to detect the rfb gene and cholera-toxin gene by multiplex-PCR. The cholera-toxin gene was detected and then we tried to isolate V. cholerae O1 and V. cholerae O139, but they were not isolated.
Keywords
Vibrio cholerae O1; Vibrio; Cholera; PCR;
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