• Korean Journal of Food Science and Technology
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• v.37 no.4
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• pp.513-518
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• 2005

Capillary electrophoresis (CE) method was developed to determine (+)-catechin and (-)-epicatechin contents in grape seed ethanol extract. CE separation was achieved using 100 mM phosphate and borate buffer at pH 6.0 as background electrolyte and fused silica capillary with 50 microns x 375 microns O.D. (effective length 20.0cm) maintained at $25^{\circ}C$. The applied voltage was 10kV, and detection was performed by DAD at 210 nm, Two catechins were well separated within 6 min with repeatability of <0.8% RSD for migration time and <2.0% RSD for peak area, and correlation coefficients higher than 0.994 were obtained from 58.0 to 174.0 mg/L with detection limit of 0.035 mg/L. Separated compounds were successfully determined. CE method was easy to handle and showed good reproducibility. CE method was compared with conventional coloring and HPLC methods, and main advantages of CE method were low amount of sample required, simple pre-sample treatment, good recovery rate, and short analysis time.

• Analytical Science and Technology
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• v.19 no.5
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• pp.369-375
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• 2006

Calibration gas mixtures were prepared using dynamic volumetric method according to ISO 6145-5 and the uncertainty was evaluated. Ten identical capillaries with 0.25 mm in inner diameter and 50 cm in length were applied in this system. Dilution ratio of parent gas was determined by the number of capillaries that passes parent gas and that passes balance gas through. Capillaries were made of Teflon which had good chemical stability against adsorption of gaseous substances. Mechanical valves were introduced in this system in order to minimize the thermal effect of solenoid valves. Concentration of prepared gases were compared with master grade standard gases in cylinders made by RiGAS Co. and calibration of the instrument were completed using comparison method according to ISO 6143. Experimental results showed that the coefficient of variance of diluted oxygen standard gases showed less then 0.2% in most dilution range, that of diluted hydrogen sulfide standard gases showed less then 1.0%. Therefore, it is proven that the standard gases prepared by this system are appropriate to be used as a calibration standards in ambient monitoring, etc.

• Proceedings of the Korean Society for Rock Mechanics Conference
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• 2000.09a
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• pp.105-115
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• 2000

As large underground projects such as radioactive waste disposal, hot water and heat storage, and geothermal energy become influential, the study, which consider all aspects of thermics, hydraulics and mechanics would be needed. Thermo Hydro-Mechanical coupling analysis is one of the most complex numerical technique because it should be implemented with the combined three governing equations to analyze the behavior of rock mass. In this study, finite element code, which is based on Biot's consolidation theory, was developed to analyze the thermo-hydro-mechanical coupling in continuum rock mass. To verify the implemented program, one-dimensional consolidation model under the isothermal and non-isothermal conditions was analyzed and was compared with the analytic solution. The parametric study on two-dimensional consolidation was also performed and the effects of several factors such as poisson's ratio and hydraulic anisotropy on rock mass behavior were investigated. In the future, this program would be revised to be used for analysis of general discontinuous media with incorporating discrete joint model.

• Tunnel and Underground Space
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• v.10 no.3
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• pp.355-365
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• 2000

As large underground projects such as radioactive waste disposal, hot water and heat storage, and geothermal energy become influential, the study, which consider all aspects of thermics, hydraulics and mechanics would be needed. Thermo-Hydro-Mechanical coupling analysis is one of the most complex numerical technique because it should be implemented with the combined three governing equations to analyze the behavior of rock mass. In this study, finite element code, which is based on Biot's consolidation theory, was developed to analyze the thermo-hydro-mechanical coupling in continuum rock mass. To verify the implemented program, one-dimensional consolidation model under the isothermal and non-isothermal conditions was analyzed and was compared with the analytic solution. The parametric study on two-dimensional consolidation was also performed and the effects of several factors such as poisson's ratio and hydraulic anisotropy on rock mass behavior were investigated. In the future, this program would be revised to be used for analysis of general discontinuous media with incorporating discrete joint model.

• Tunnel and Underground Space
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• v.21 no.1
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• pp.66-81
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• 2011

In this study, it was aimed to develop a thermal-hydraulic-mechanical coupled fracture mechanics code that models a fracture initiation, propagation and failure of underground rock mass due to thermal and hydraulic loadings. The development was based on a 2D FRACOD (Shen & Stephasson, 1993), and newly developed T-M and H-M coupled analysis modules were implemented into it. T-M coupling in FRACOD employed a fictitious heat source and time-marching method, and explicit iteration method was used in H-M coupling. The validity of developed coupled modules was verified by the comparison with the analytical result, and its applicability to the fracture initiation and propagation behavior due to temperature changes and hydraulic fracturing was confirmed by test simulations.

• Proceedings of the KIEE Conference
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• 1998.07c
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• pp.1140-1142
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• 1998

This paper presents object-oriented dynamic programing formulation of the unit commitment problem. This approach features the classification of generating units into related groups so called class. All object which share the same set of attributes and methods are grouped together in classes and designed inheritance hierarchy to minimize the number of unit combination which must be tested without precluding the optimal path. So this programming techniques will maximize the efficiency of unit commitment.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2006.05a
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• pp.20-24
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• 2006

In this paper we investigated the method of examining the accelerated life test on LED traffic signal module by the temperature. The longevity presumption of the LED type signal light by a general heat cycle test used and executed cycle when it was done to longevity by the heat cycle test number and the acceleration factor of a real system requirements of this heat cycle test. Therefore, it reports on the introduction of the acceleration type from which the LED traffic signal module is done here to clarify the above-mentioned acceleration factor with the object and the acceleration factor is requested the test atmosphere actually in the system requirements.

• Journal of fish pathology
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• v.12 no.1
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• pp.24-31
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• 1999

Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified $50{\mu}g/ml$ of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of $50{\mu}g/ml$ of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were $1{\times}10^5$ cells/ml and $1{\times}10^5$ cells/ml expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.

• Membrane Journal
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• v.31 no.5
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• pp.363-370
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• 2021

Organic solvent resistant hollow fiber membranes were fabricated via a thermally induced phase separation (TIPS) method using Polyketone polymer, a material with excellent resistance to organic solvents. The PEG300, DMSO₂ and Glycerine called the "green solvents" were used as diluents for TIPS method. The spherulite structure was formed with DMSO₂ by S-L phase separation behavior whereas the bicontinuous structures were formed with PEG300 and Glycerine, respectively. The morphology of the PK hollow fiber membranes was investigated using SEM. The pure water permeability and the durability test were conducted to understand the permeation properties of PK hollow fiber membranes. The tensile strength test was conducted for the property of mechanical strength. In this study, the fabrication of PK hollow fiber membranes with various diluents was discussed to understand the correlation between diluent and polymer in detail.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.185-192
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• 2018

The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ${\geq}0.3$ O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.