• Journal of Mushroom
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• v.19 no.3
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• pp.210-215
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• 2021

For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.97-105
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• 2023

Concentration of nitrogen, one of the major elements, and ratio of two nitrogen forms (NH₄⁺ and NO₃^-) in the nutrient solution affect the quality and food safety of fresh vegetable produce. This study was conducted to find an appropriate strength and NH₄⁺:NO₃^- ratio of a nutrient solution for growth and development of a Romaine lettuce (Lactuca sativa L. var. longiflora) 'Caesar Green', a representative leafy vegetable, grown in a home hydroponic system. In the first experiment, plants were grown using three types of nutrient solution: A commercial nutrient solution (Peters) and two strengths (GNU1 and GNU2) of a multipurpose nutrient solution (GNU solution) developed in a Gyeongsang National University lab. Plants grown with the GNU1 and GNU2 had greater shoot length, leaf length and width, and biomass yield than Peters. On the other hand, the root hairs of plants grown with Peters were short and dark in color. Tissue NH₄⁺ content in the Peters was higher than that of the GNU1 and GNU2. The higher contents of NH₄⁺ in this solution may have caused ammonium toxicity. In the second experiment, eight treatment solutions, combining GNU1 and GNU2 solutions with four ratios of NO₃^- :NH₄⁺ named as 1, 2, 3 and 4 were used. Both experiments showed more growth in the GNU2 group, which had a relatively low ionic strength of the nutrient solution. The growth of Romaine lettuce showed the greatest fresh weight along with low tissue NO₃^- content in the GNU2-2. This was more advantageous in terms of food safety in that it suppressed the accumulation of surplus NO₃^- in tissues due to the low ionic trength of the GNU2 subgroup. In addition, this is preferable in that it can reduce the absolute amount of the input of inorganic nutrients to the nutrient solution.

• Journal of Life Science
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• v.28 no.7
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• pp.819-826
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• 2018

Orostachys japonicus (OJ) is a medicinal herb with immunoregulatory, anti-aging, anti-oxidative, and many other therapeutic properties. The purpose of this study was to elucidate the anti-cancer property of cultivated OJ. SW480 cell viability was significantly reduced by cumulative exposure to OJ extract. We also observed inhibitory effects of OJ after 72 hr through the growth and migration of SW480 cells using scratch assay. SW480 cells in OJ-free medium began to move into the scratch site at 24 hr; however, cells in medium containing OJ did not migrate into the scratch site until 48 hr. Male C57BL/6 mice (4 weeks old) were orally administered OJ extract for 31 days before injection of SW480 cells. At 7, 14, and 28 days after subcutaneous injection of SW480 cells, tumor weight and volume were analyzed. The body weight of the OJ-treated group was continuously increased during administration of the OJ extract relative to the control group. Injection of SW480 cells caused a reduction in body weight in all groups; however, the OJ-treated group exhibited a significant increase in body weight after 14 days. Tumor weight and volume were lower in the OJ-treated group than in the control group after 28 days. Although these results suggest that OJ suppresses the proliferation and migration of human colon cancer cells, additional studies are required to provide preclinical evidence before launching clinical trials evaluating OJ as an anti-cancer biohealth product.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.293-298
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• 2014

This study conducted experiments on the reclaimed land of Saemangeum located in Jeongrabuk-do in order to gain basic information about growth characteristics and yield ability according to soil salinity. Having soil excluding salt as a control group, this study adjusted the specimens' soil salinity to level 4 and then planted four varieties including Ilmichal Corn to investigate the growth or grain yield according to salinity. About the corn establishment rate according to soil salinity, over 97% up to $3.2dS\;m^{-1}$ was established, and then, it was reduced gradually according to the increase of concentration. According to the salt concentration of soil more required growth duration from seeding to heading comparing to non-treatment salt was delayed, at $1.6dS\;m^{-1}$, two days were delayed, at $3.2dS\;m^{-1}$, four to six days were delayed differently by varieties, and at $4.8dS\;m^{-1}$, six to 10 days were delayed. About the plant fresh weight according to soil salinity, Chalok 4 and Eolrukchal indicated 93%~97% or so compared with the salt-free one at $1.6dS\;m^{-1}$, and Chalok No. 4 showed 79% at the salinity of $3.2dS\;m^{-1}$, too, and it was a relatively higher growth percentage than those of the other varieties. In terms of dried seed weight according to soil salinity, compared with the corns cultivated in the control group, averagely 12.1% was lowered at the time of cultivation at $1.6dS\;m^{-1}$, and $3.2dS\;m^{-1}$ 40% was lowered, and about 70% was lowered at $4.8dS\;m^{-1}$. According to the result of examining the point of time that dried seed start to reduce due to soil salinity with the regression equation, soil salinity which starts the reduction of grain weight is $1.67dS\;m^{-1}{\sim}2.18dS\;m^{-1}$, and it differs by varieties, and EC of 50% that the yield reduces in half is $2.96dS\;m^{-1}{\sim}4.45dS\;m^{-1}$. And the degree of influence on each of the growth factors according to soil salinity is founded to be in the order of establishment rate

• Horticultural Science & Technology
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• v.34 no.6
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• pp.845-859
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• 2016

This study was conducted to examine the effect of nighttime ventilation and heating on changes in temperature and humidity, the occurrence of white rust, and growth of standard chrysanthemum (Dendranthema grandiflorum) 'Baekma' in a greenhouse. For the ventilation treatments, the mean nighttime humidity in the control greenhouse, which had a closed side window, was higher (94.5%), but the humidity in the natural and natural+fan ventilation treatments was lower (74.3% and 72.8%, respectively). The rate of occurrence of white rust at 34 days after treatment was 100, 98.3, 75.6, and 43.3% for the control, fan ventilation, natural ventilation, and natural+fan ventilation treatments, respectively. The number of infected leaves and telia were the lowest in the natural+fan ventilation treatment compared with the other treatments. The growth of the chrysanthemum 'Baekma' was significantly suppressed in the control because of the occurrence of white rust and high humidity, while plant height, number of leaves, stem diameter, and fresh weight were the greatest in the natural+fan ventilation treatment. For the heating treatments, the mean nighttime temperature of the control (RH 95% heating), which was heating and held at 95% humidity, was $18.4^{\circ}C$, while the temperature of the heating treatment, which was held at 70% relative humidity (RH 70% heating) was $25.8^{\circ}C$. The rate of occurrence of white rust (34.4%), number of infected leaves (0.9), and telia (1.0) were the lowest in the RH 70% heating treatment compared with the other heating conditions. Also, the RH 70% heating treatment showed the best growth in terms of plant height, stem diameter, number of leaves, and fresh weight. Therefore, the natural+fan ventilation and RH 70% heating treatments were effective for the control of white rust and the growth of standard chrysanthemum 'Baekma' in a greenhouse.

• Korean Journal of Environmental Agriculture
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• v.20 no.3
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• pp.180-185
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• 2001

Control effects of phosphorous acid were investigated on Phytophthora blight of red pepper plants in greenhouse and field. In vitro test, the mycelial growth of Phytophthora capsici was inhibited by the phosphorous acid more than 97% at 1,000 ${\mu}g$ and 10,000 ${\mu}g$ a.i./mL in the liquid and solid culture, respectively. At concentration of 10 ${\mu}g$ a.i./mL of phosphorous acid, in the liquid culture the mycelial growth of P. capsici was inhibited 46.2%, however inhibited only 4.9% on the soild culture. Zoosporangial formation was also inhibited 89.1% by phosphorous acid at 100 ${\mu}g$ a.i./mL. Phosphorous acid affected more zoosporangial formation of P. capsici than its mycelial growth. At the concentrations of 10,000, 1,000 and 100 ${\mu}g$ a.i./mL of phosphorous acid, germination of zoosporangia was inhibited 100, 84.3 and 44.2%, respectively. Mycelial growth and zoosporangial formation of P. capsici were little affected at the concentration of 10 ${\mu}g$ a.i./mL of phosphorous acid. Cermination of zoosporangia was also little affected at this concentration, however growth of the germ tubes was inhibited and the abnormal mycelial growth was observed. Phosphorous acid suppressed the incidence of Phytophthora blight of red pepper plants up to $77.0{\sim}62.0%$, in greenhouse. Phosphorous acid suppressed the incidence of Phytophthora blight of red pepper plants up to 54.0% at the conventional culture in field. Treatments of phosphorous acid increased up to 113% in height, 135% in number of fruit, and 129% in weight of fruit.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.317-323
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• 1993

In order to clarify the contents of active principles in floral inhibition cultured Angelica gigas roots, the amounts of crude extract and the contents of decursin and decursinol angelate in the yearly roots were analyzed and compared with respect to the root age, root part and growth stage. The woody cell, weight and width of pith and cortex were also investigated at different growth stage to observe the developmental characteristics of lignification in the roots as bolting and flowering in normal cultured Angelica gigas. The amount of crude extracts did not differ with plant ages, whereas the contents of decursin and decursinol angelate were differed and the highest in 3 year old roots. The contents of decursin in 1, 2 and 3 year old roots were 3.71, 4.76 and 8.20% and those of decursinol angel ate were 2.84, 3.40 and 5.01%, respectively. The amount of crude extracts, and the contents of decursin and decursinol angelate were the highest in fine roots, followed by the lateral roots and the lowest in the primary roots. On the other hand, the amount of the constituents in the cortex were much higher than those in the pith of the root. The amounts of crude extract, and the contents of decursin and decursinol angelate showed the highest value at the vagetative stage and decreased with development to bolting and blooming stage. Woody cells were accumulated in the pith of the root as advancing growth stage, so that the weight and radius of the pith increased, whereas the relative weight and width of the cortex decreased slightly.

• Korean Journal of Organic Agriculture
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• v.14 no.3
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• pp.315-323
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• 2006

The potential of the predatory mite Phytoseiulus persimilis (Acarina : Phytoseiidae) to control two spotted spider mite Tetranychus urticae (Acarina : Tetranychidae) was investigated on sustainable strawberry fields in Jeonnam area. The density of T. urticae increased from 4th and 7th weeks after single and two timings release P. persimilis, respectively. On the three timings of release plot, number(density) of T. urticae on a leaf maintained fewer than 10 during the 15 weeks after release. In the 1,000 release of P. persimilis per $100m^2$ plot, P. persimilis could not suppress T. urticae. In the 2,000 release plot, P. persimilis could suppress T. urticae with low density which was the similar in the 3,000 release plot. Two-spotted spider mite, T. urticae, occurred from late March and increased density in Hampyeong area. In the P. persimilis released field, T. urticae inhibited continuously after release. In Boseong area, density of P. persimilis increased 50 per 10 leaves through increasing of T. urticae. Percent of occurrence of T. urticae showed high $10.5{\sim}75.5%$ in none-release predatory mite but not high in release treatment. T. urticae on sustainable strawberry field could be inhibited by P. persimilis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.216-219
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• 2000

A field study was carried out to compare the performances of mulching with recycled paper, oil-treated paper and urea-coated paper in spring season potato culture. Soil temperature, weed occurrence, and the growth and yield of potato (cv. Sumi) were compared with unmulched control. The average soil temperatures at 5cm soil depth during the first 10 days after planting were 18.9$^{\circ}C$, under paper mulch and 20.6$^{\circ}C$, under oil-treated paper mulch, being lower 0.8$^{\circ}C$ and higher 1.2$^{\circ}C$, respectively, than unmulched control. Paper mulch lowered maximum soil temperature by 4.6$^{\circ}C$, on a fine day, while oil-treated paper mulch elevated it by over 6.6$^{\circ}C$, Urea-coated paper was decomposed fester than the mulch paper without urea coating. The former was decomposed 50% by 80days after mulching, but the later only 20%. In all mulch treatments, weeds were effectively controled throughout the potato growing season. The percent emergence of potato was not different significantly among treatments. However, a little lower emergence and poor early growth were observed under oil-treated paper mulch because of higher soil temperature. Except oil-treated paper mulch, the paper mulches with and without urea coating showed no difference in growth and yield of potato from the unmulched control. The growth and yield performances were poorest under oil-treated paper mulch. Oil-treated paper mulch would result in difference growth and yield performances if potato is planted earlier than April IS in the present experiment. Further researches are needed for this aspect.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.304-311
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• 2009

This study was conducted to develop functional sprout vegetables with antioxidant effects using seeds of Arctium lappa. The seeds germinated vigorously under light at $25^{\circ}C$, reaching germination rate of 82% within 4 days. Germinated seeds were placed under darkness at various temperatures to force growth in length, and it was demonatrated that $20^{\circ}C$ was optimum temperature. Greening treatment reduced growth in length, but promoted growth of cotyledons. Harvested A. lappa sprout vegetables maintained freshness longer at $10^{\circ}C$, rather than $4^{\circ}C$. Ventilation holes in storage containers had no effects on storage periods. Antioxidant activity of vegetable that received greening treatment for 1-3 days was investigated, and it was shown that free radical scavenging effects and ferrous ion chelating effects was higher than those of commercially available brocoli, cauliflower, pea and bean sprout. Contents of total polyphenol and flavonoid were also higher, especially by 3 day greening. The longer the treatment, the more the inhibition on peroxidation of linoleic acid. Sprout vegetable of A. lappa had higher antioxidant activity compared with adult plant. In conclusion, sprout vegetable of A. lappa has great potentiality for use as one of sprout vegetables.