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http://dx.doi.org/10.14480/JM.2021.19.3.210

Efficacy of Listeria Innocua Reduction on Enoki Mushrooms by Utilization of an Air Sterilization Device  

Lee, Hyun-Dong (Smart Farm Development Division, National Institute of Agricultural Science, RDA)
Yu, Byeong-Kee (Smart Farm Development Division, National Institute of Agricultural Science, RDA)
Seo, Da-Som (Smart Farm Development Division, National Institute of Agricultural Science, RDA)
Kim, Se-Ri (Microbial Safety Division, National Institute of Agricultural Science, RDA)
Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kwak, Kang-Su (Smart Farm Development Division, National Institute of Agricultural Science, RDA)
Publication Information
Journal of Mushroom / v.19, no.3, 2021 , pp. 210-215 More about this Journal
Abstract
For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.
Keywords
Enoki mushroom; Listeria monocytogenes; Listeria Innocua; Ion-cluster; UV;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Yang CW. 2021. Inspection of Listeria monocytogenes in Enoki mushrooms distributed in Korea. Master Thesis. Korea University, Seoul, Korea (in Korean).
2 J The korean institute of Electrical and electronics Material Eng, Hong YW. 2010. Thick film type cluster in Mg2SiO4/glass composite ceramics for anion generation. J Korean Inst Electr Electron Mater Eng 23: 118-123 (in Korean).   DOI
3 Lee JE, Kim SA, Shim WB. 2018. Reduction measures and contamination status of Listeria monocytogenes among fresh produce. Safe Food 13: 24-33 (in Korean).
4 Lee KW, Jeon JO, Lee KJ, Kim YH, Lee CJ, Jang M-J. 2019. Analysis of growth environment of Flammulina velutipes using the smart farm cultivation technology. J Mushrooms 17: 197-204 (in Korean).
5 KATI (Korea Agro-Fisheries & Food Distribution Corporation). 2020. Export statistics of Enoki mushroom. Available at: https://www.kati.net.
6 Centers for Disease Control and Prevention. 2020. Outbreak of Listeria infections linked to enoki mushrooms. Available at: https://www.cdc.gov/listeria/outbreaks/enoki-mushrooms-03-20/index.html/.
7 Food Safety Authority of Ireland. 2020. 1st Trimester National Microbiological Survey: Microbiological safety/quality of raw mushrooms. Available at: https://www.fsai.ie/uploadedfiles/raw_mushrooms.pdf.
8 Jee YJ. 2010. Cluster ion generation technology for a comfortable indoor environment. Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea 39: 17-23 (in Korean).
9 Hu M, Gurtler JB. 2017. Selection of surrogate bacteria for use in food safety challenge studies. J Food Prot 80: 1506-1536.   DOI
10 Kim SR, Kim WI, Yoon JH, Jeong DY, Choi SY, Hwang IJ, Nagendran R. 2020. Growth survival of Listeria monocytogenes in Enoki mushroom (Flammulina velutipes) at different temperatures and antilisterial effect of organic acids. J Food Hyg Saf 35: 630-636 (in Korean).   DOI
11 Saunders T, Wu J, Williams RC, Huang H, Ponder MA. 2018. Inactivation of Salmonella and surrogate bacteria on cashews and macadamia nuts exposed to commercial propylene oxide processing conditions. J Food Prot 81: 417-423.   DOI
12 Ministry of Agriculture, Food and Rural Affairs. 2019. Cash crop production records.
13 Jhune CS, Leem HT, Yun HS, Kong WS, Cho JH, Sung GH, Lee CJ. 2010. The influence on cultivation characteristics of fruiting body of winter mushroom by growing humidity. J Mushrooms 8: 116-121 (in Korean).
14 KDCA (Korea Disease Control and Prevention Agency Infectious Disease Portal). Listeria monocytogenes infection. Available at: http://www.kdca.go.kr/npt/biz/npp/portal/nppSumryMain.do?icdCd=ND0611&icdgrpCd=04&icdSubgrpCd=ND0006.
15 Lee HH, Kwon YL, Cho YM. 2020. A study on the effect of ventilation and ion cluster on the distribution of bioaerosols in multi-use facilities. In Abstracts from the Meeting of the Korean Society for Atmospheric Environment. pp. 168 (in Korean).
16 Pennone V, Lehardy A, Coffey A, Mcauliffe O, Jordan K. 2018. Diversity of Listeria monocytogenes strains isolated from Agaricus bisporus mushroom production. J Appl Microbiol 125: 586-595.   DOI