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Efficacy of Listeria Innocua Reduction on Enoki Mushrooms by Utilization of an Air Sterilization Device

공기 살균 장치 적용 팽이버섯 재배사의 Listeria Innocua 저감 효과

  • Lee, Hyun-Dong (Smart Farm Development Division, National Institute of Agricultural Science, RDA) ;
  • Yu, Byeong-Kee (Smart Farm Development Division, National Institute of Agricultural Science, RDA) ;
  • Seo, Da-Som (Smart Farm Development Division, National Institute of Agricultural Science, RDA) ;
  • Kim, Se-Ri (Microbial Safety Division, National Institute of Agricultural Science, RDA) ;
  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwak, Kang-Su (Smart Farm Development Division, National Institute of Agricultural Science, RDA)
  • 이현동 (국립농업과학원 스마트팜개발과) ;
  • 유병기 (국립농업과학원 스마트팜개발과) ;
  • 서다솜 (국립농업과학원 스마트팜개발과) ;
  • 김세리 (국립농업과학원 유해생물과) ;
  • 이찬중 (국립원예특작과학원 버섯과) ;
  • 곽강수 (국립농업과학원 스마트팜개발과)
  • Received : 2021.09.15
  • Accepted : 2021.09.17
  • Published : 2021.09.30

Abstract

For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

팽이버섯 재배사의 Listera속 미생물 살균을 위하여 공기 살균 장치가 부착된 파일럿 버섯 재배사를 개발하여 물리적, 화학적 살균처리에 대한 살균 효과 검증실험을 수행하였다. 파일럿 버섯 재배사의 내부 온도는 상부 6.62℃±0.30, 중간 6.46℃±0.24, 하부 6.48℃±0.25, 습도는 79.97%±4.42, 79.43%±4.06, 79.94±4.30%로 설정 온도 6.5℃, 상대습도 75%에 근사하게 제어되었다. 공기 살균 장치 적용에 적합한 팽이버섯 재배단계는 생육단계 조건인 온도 6.5~8.5℃, 습도 70~80% 범위였고 유사 조건에서 이온 클러스터 발생기의 오존 발생농도는 160 ppb 수준으로 나타났다. 물리적 살균처리 후 Listeria innocua의 생존율은 이온클러스터 살균의 경우 0.1~0.9%, UV공기 살균은 9.3~10.6%로 나타났고, 화학적 살균처리인 75% 에탄올과 3% 유기산 수용액 처리구에서는 모두 사멸하는 것으로 나타났다. 소재에 대한 Listeria innocua 생존율은 금속시편의 경우 9.3~10.6%, 플라스틱 권지 9.9~16.2%로 나타났는데, 특히 권지의 거친면에서 생존율이 높게 나타났다. 본 연구 결과에 따르면 버섯 재배사의 Listeria균 발생을 억제하기 위해서 금속 소재로 구성된 재배사 벽면과 재배 선반에 대해서는 이온클러스터 공기 살균처리가 노동력을 절감하면서 살균 가능한 방법이며, 플라스틱 재질의 권지의 경우 화학적 살균처리가 효과적인 것으로 나타났다.

Keywords

Acknowledgement

이 연구는 농촌진흥청 연구사업(세부과제번호: PJ01414201, 팽이버섯 재배사 친환경 살균기술 개발)의 지원에 의해 이루어진 것입니다.

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