• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.295-305
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• 1994

Greenhouse and laboratory studies were conducted to investigate the allelopathic potential of alfalfa and vetch residues on soybean and corn using various extract concentrations (0, 5, 10, 15 and 20%, w/v) and residue rates (0, 0.25, 0.5, 0.75 and 1%, w/w). Aqueous extracts of alfalfa (Medicago sativa L.) and vetch (Vicia spp.) exhibited an allelopathic effect on soybean and corn seed germination, seedling length and weight. The degree of inhibition significantly increased as the aqueous extract concentration increased. Alfalfa and vetch 20% extracts reduced soybean seed germination, seedling length and weight by 35%, 57%, 32% and 15%, 42%, 25% respectively, when compared to control. Corn germination, seedling length and weight was inhibited by 20%, 23%, 38% by alfalfa and 19%, 18%, 35% by 20% vetch extracts. Alfalfa and vetch extracts inhibited secondary root formation and branching as the extract concentration increased. Alfalfa and vetch 20% extracts inhibited by 41% and 32% secondary root numbers, respectively as compared to control. It was found that the aqueous extract of alfalfa resulted in greater reduction in germination, seedling length and weight of soybean than that of vetch. Alfalfa and vetch 1% residue rate inhibited soybean plant height by 30% and 10%, leaf area by 31% and 23%, and dry weight by 18% and 1%, nodule number by 27% and 20% also. Alfalfa and vetch residue significantly enhanced plant height, leaf area and dry weight of corn. The maximum stimulation occurred with 0.25% and 1% of alfalfa and vetch residue rates, respectively. Plant height, leaf area, and dry weight increased by 23%, 59%, 58% and 17%, 52%, 94% with alfalfa and vetch residues of 0.25% and 1%, respectively. This study demonstrates that there is an allelopathic potential resulting from alfalfa and vetch residues on soybean growth and yield. It also suggests that these residues may affect crop growth and development due to the inhibitory or stimulatory effects of allelochemicals existing in the residue.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.179-184
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• 2017

Tolaasin, a 1.9 kDa peptide toxin, is produced by Pseudomonas tolaasii and causes the brown blotch disease of cultivated oyster mushroom. It forms pores on the membrane and thus destroys cellular membrane structure, seriously reducing the productivity of mushroom cultivation. The mechanism of tolaasin-induced cytotoxicity is not known in detail. However, it has been reported to form a pore structure in the cytoplasmic membrane through the molecular multimerization. Therefore, food additives which can interact with tolaasin molecules may inhibit the pore formation by hydrophobic interactions with tolaasin molecules. In this study, various food additive materials have been identified as inhibitors of the tolaasin activity and named tolaasin-inhibitory factors (TIF). Most of TIFs are emulsifying agents for food processing procedures. Among various TIFs, polyglycerol and sucrose esters of fatty acids blocked effectively the cytotoxicity of tolaasins at the concentrations $10^{-4}-10^{-5}M$. These TIFs also successfully suppressed the blotch disease development in the shelf cultivation of oyster mushroom.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.41-46
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• 2016

Bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli is defective disease to watermelon cultivated areas. To control of BFB, we investigated control efficiency to use commercial antibacterial pesticides. Growth inhibition zone on medium were formed as oxolinic acid WP and oxytetracycline WP. Control efficacy of four anti-bacterial pesticides on seed and seedling stage were performed. As a results, oxytetracyclin WP is shown over 90% control efficiency on seed and acibenzolar-S-methyl + mancozeb WP shown over 90% control efficiency on seedling stage Hot-water treatment method could be possible to reduced infection rate on seed. The conditions of hot-water treatments are $50{\sim}55^{\circ}C$ on 20~30 minutes. These results suggested that the methods were helpful watermelon seedling nursery to control of the bacterial fruit blotch by A. avenae subsp. citrulli.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.207-216
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• 2003

Most severe problem in production of potted kalanchoe during summer season is retardation of growth caused by high temperature. The aim of this experiment was aimed to investigate the effects of natural products such as algin-oligosacchride and glucosamine oligosaccharide, plant growth promoting rhizovacteria such as Pseudomonas sp. B and Pseudomonas sp. D2, and AG-solution on the growth of potted kalanchoe under the different root zone temperature in the greenhouse. Growth characteristics in terms of plant height, leaf length, leaf width, leaf area, leaf weight, fresh weight of shoot and root and root length were recorded under three root zone temperatures (25$^{\circ}C$, 30$^{\circ}C$, 35$^{\circ}C$). In 25$^{\circ}C$, the mixed treatment of Pseudomonas sp. B and glucosamine oligosaccharide resulted in the best growth in terms of plant height, leaf area and root weight. In 3-$^{\circ}C$, glucosamine oligosaccharide treatment gave fair result in plant height and leaf weight, but the mixed treatemtn of Pseudomonas sp. D2 and algin-oligosaccharide showed better growth on leaf area and root weight. In 35$^{\circ}C$, the mixed treatment of Pseudomonas sp. B and glucosamine oligosaccharide could greatly improve the plant height, leaf area, leaf weight and root weight. These results demonstrated that the mixed treatment of natural products and microorganisms could overcome the detrimental effects caused by high temeprature in the production of kalanchoe.

• Korean Journal of Organic Agriculture
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• v.19 no.2
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• pp.245-253
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• 2011

This study was carried out to investigate the biological effects of organic soybean extracts. Cellular and molecular analysis was performed to determine anti-oxidative, anti-inflammatory, anti-apoptotic, and anti-inflammatory effects of soybean extracts. First, we obtained various solvent extracts of soybean such as water, ethanol, and methanol. Molecular and cellular analysis were performed with 0.1 mg/ml concentration of each solvent extracts. The results of anti-oxidative, antiinflammatory and anti-apoptotic effects of organic soybean extracts were prominent. However, organic soybean extracts were not observed in anti-allergic effects determined by releasing histamine from rat mast cell line, RBL-2H3. Conclusively, organic soybean suppress inflammatory responses. In addition, organic soybean could be applied as a functional food ingredient for treatment of chronic inflammation, asthma, and atopic dermatitis with enhanced anti-inflammatory activities.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.44-48
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• 2015

This study was conducted to evaluate the efficacy of weed control and phytotoxicity of oxyfluorfen using a transgenic rice line (CPPO06 event) resistant to protoporphyrinogen oxidase (Protox) inhibiting herbicides in the direct-seeding flooded rice. Five annual weeds including Echinochloa oryzoides and two perennial weeds were occurred in the test field. Oxyfluorfen at 120 g a.i. $ha^{-1}$ in the application timing of two days before sowing and 0 and five days after sowing showed more than 90% weed control value except for Juglans mandshurica. Total weed control value was more than 95% in any application timing indicating a highly effective herbicide in the direct-seeding flooded rice. When compared with untreated control, no visual injuries were detected at single and double dosage of oxyfluorfen. The agronomic characteristics and yield components of CPPO06 event was reasonable in any time of application in this study. Based on these data, oxyfluorfen application before and after sowing can be applied to provide effective weed management in the direct-seeding flooded field.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.72-79
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• 2011

Here we report a simple screening system using hematoxylin staining (HS) of the root apex. It allowed rapid classification into different aluminum (Al) tolerance from 65 cultivars within one week. Using this system, we selected the most Al-tolerant (Jayae-2) and-sensitive (Pum-2) barley (Hordeum vulgare L.) The results show that the different responses in Al-induced growth inhibition, Al accumulation and expression of plasma membrane (PM) $H^+$-ATPase in root apices of selected two cultivars. It showed strongly Al-induced growth inhibition in a dosedependant manner only in Pum-2 but not in Jayae-2. Aluminum accumulation in root apices (10 mm) was significantly higher in Pum-2 only. The $H^+$-ATPase expression of PM vesicles by western blotting was decreased in Pum-2 but not in Jayae-2 treated with $20{\mu}M$ Al for 24 h. These finding indicate to screen from our system is rapid and reliable and to sustain the expression of PM $H^+$-ATPase at translational level is an important role in root growth as affected by Al.

• Korean journal of applied entomology
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• v.23 no.1 s.58
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• pp.22-27
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• 1984

The effects of solarization on the suppression of soilborne plant pathogen and the growth promotion of cucumber plants were examined in artificially infested soil by vinyl mulching and not mulching from July 25 to August 25, 1983. During the solarization period, the highest temperatures were $58^{\circ}C,\;45^{\circ}C,\;and\;42^{\circ}C$, at 5cm, 15cm, and 25cm of soil depth respectively. The inoculum of cucumber wilt pathogen, Fusarium oxysporum f. sp. cucumerinum, was mixed with soil 30cm deep and saturated with water. The pathogen was completely killed after 30dys of solarization in 5cm soil depth and 98 percent of inoculum was eliminated in 15cm soil depth. But the survival rate of the fungi in 25cm soil depth of solarized plot did not show significant differences compared with those in nontreated plot in 5cm and 15cm depth. Although some of the pathogenic fungi might survive from solarized soil in 15cm and 25cm depth, the ability of microconidia production was reduced significantly The number of microconidia grown on Komada's medium in isolates the primary colonies from solarized soil was less than that in isolates from nontreated soil approximately by one fourth. The first subcultured solates from the solarized soil grown on potato dextrose agar also produced a small amount of microc. onidia compare with that of subcultured isolates from nontreated soil. Cucumber seedlings planted in the soil collected from solarized plot grew much better than that in the soil from nontreated plot at any of soil loved, especially in 5cm of soil depth. And the fruits harvested from cucumber plants grown in the solarized plot were more in number and leavier in weight than that from nontreated plot. Besides the typical symptom development, significant growth suppression wvas recognized with increase of inoculum density of F. oxysporum f. sp. cucumerinum at early stage of cucumber seedlings in steam sterilized soil.

• Korean journal of applied entomology
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• v.25 no.3 s.68
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• pp.169-173
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• 1986

Ginseng fields abolished by potato rot nematode (Ditylenchus destructor) (heavily infested with the nematode) were treated with selected nematicides after preplanting treatment with fumigants or non fumigant chemicals in the fall of 1982. Suppression of the nematode populations was noted in the fields fumigated by cylon (Cy) and treated with ethoprop (E) or triazophos (T). Also in these treatments, high percentages of shoot sprouting and low nematode damages were observed. In the fields fumigated by Cy, subsequent treatments with nonfumigant nematicides such as E, aldicarb (A), phenamiphos (Ph), oxamyl (O) and carbofuran (C) during cultivation had no effect on the significant decrease of the nematode, compared with Cy alone. No differences in shoot sprouting and symptom appearance were observed; however, survivalities of plants and root weights of 4-year old ginseng were high in A and E or E following preplanting treatment with E. Correlation coefficients of the nematode densities to host responses such as sprouting of shoots, symptom appearance, survivality of plants and weight of 4-year old ginseng roots were significant at and mostly before and after the time periods of survey.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.4
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• pp.389-394
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• 2011

This experiment was carried out to find effective weed control method of henbit deadnettle ($Lamium$ $amplexicaule$ L.) on cultivating field of Italian ryegrass ($Lolium$ $multiflorum$ Lam) using herbicide in Grassland and Forages Division, National Institute of Animal Science, RDA, Cheonan from 2008 to 2009. When Onehof was treated two times of spray, weed control ratio was most high as 90%, but this method can not be used because of much damage to Italian ryegrass. When MCPP was treated two times of spray, weed control ratio was high as 82%, and damage to Italian ryegrass was very insignificant. Especially, when the spray of MCPP was treated two times, dry matter yield of Italian ryegrass was 11,427 kg/ha, but that of non treatment was 1,658 kg/ha. That is to say, forage harvest was impossible in non treatment field. According to these results, to control henbit deadnettle on cultivating field of Italian ryegrass using herbicide, you need to treat with two times of spray of MCPP in mid-March. If you do, you can get regular harvest as much as 11,427 kg/ha.