• Proceedings of the Korean Society of Sericultural Science Conference
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• 2004.04a
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• pp.28-28
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• 2004

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.186-188
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• 1996

곤충애 있어서 호르몬의 작용기작과 그 이용에 관하여 요야하면 다음과 같다. 곤충호르몬에는중추신경계의 신경분비세포에서 합성, 분비되는 peptide의 neuropeptide hormone (PTTH, bombyxin, diapause hormone등)이 있고, 상피계의 내분비선에서 합성, 분비되는 sesquiterpene의 유약호르몬 ( juvenile hormone)과 steroid의 탈피호르몬 (ecdysone)이 있다. 곤충호르몬은 특정한 표족세포에 있는 수용체와 높은 특이성과 높은 친화성을 가지고 결합해서 세포의 작용을 조절한다. 일반적으로 peptide hormone은 표적세포의의 세포막을 통과할 수 없으므로 표적세포의 막표면에 있는 수용체와 결합하는 것에 의해 세포내 대사제를 활성화시킴으로서 peptide hormone의 특이적인 발현이되는 것으로 알려지고 있다. 한편, ecdysone과 같은 steriod hormone이나 juvenlie hormone은 표적세포의 세포막을 용이하게 통과할수 있으므로 세포내의 세포막을 용이하게 통과할 수 있으므로 세포내로 들어가 수용체와 결합해서 hormone-receptor comlpex는 핵내로 들어가 DNA의 특이적인 영역에 결합하므로서 이들 호르몬 특이적인 기능이 발현되는 것으로 알려져 있다. Ecodysone의 활성이 있는 ecdysteroid가 여러 식물에서 발견되고 있어, 금후 양잠의 상족에 이용이 기대되고 있다. 또한, 향유약호르몬(AJH) 활성물지인 imidazole계 화합물은 양잠에 있어서 세섬도고치 생산에 그 이용이 기대되고 있다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2002.04a
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• pp.95.1-95
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• 2002

• Journal of Sericultural and Entomological Science
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• v.14 no.1
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• pp.25-35
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• 1972

This investigation was carried out to find the effects of silkworm rearing technique on the harvesting amounts. In spring and autumn of 1971, 14 silkworm rearing farmers at Botong Ri and Youngsoo Ri, Chongnam Myun, Hwaseung Kun, Kyunggi Do in Korea were selected as sample farmers. They were devided into two groups, high productivity group and low productivity group according to their harvesting amounts per box of silkworm eggs in 1970. The high productivity group represents the farmer who produced above average harvesting amounts per box, and the low group represents below average group. In this investigation, all farmers were adjusted to share uniform rearing condition with even mulberry leaves. Under this uniform condition, the effects of rearing techniques on the harvesting amounts per box of silkworm eggs and the effects of rearing condition on the harvesting amounts were inferred. Results obtained are as fellows; (1) Measuring from the 14 sample farmers, the effect of rearing technique on the harvesting amounts per box covers 15% of farmers in spring rearing season but 33% in autumn season. (2) Measuring from these sample farmers, the effects of the nutrition condition is less than 56% in spring rearing season but less than 69% in autumn, and it can be concluded that these effects include the effect of climate factor. (3) In the low productivity group. the most important facto. affecting the low productivity is non-disinfection of rearing environment.

• Journal of Environmental Science International
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• v.32 no.11
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• pp.833-840
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• 2023

Insect-healing program is designed for farm use, and this study attempted to scientifically investigate its healing effect based on application methods. The insects used in the program are Trypoxylus dichotomus, Papilio xuthus, and Bombyx mori. Insect-healing program was conducted on 61 adults of over 50 years old and at two different farms for 12 weeks. Stress, anxiety, insomnia, and cognitive function were measured through pre- and post-surveys. Result of the analysis showed that cognitive function changed positively in direct experienced group (those that encountered live insects) and indirect experience group (those that encountered insects only through photos and videos). However, psychological variables such as stress and anxiety decreased and sleep time increased significantly only in the direct experienced group. These results demonstrate that an agro-healing program that involve the use of insect must include activities such as raising and taking care of live insect to achieve positive psychological healing effects.

• Journal of Life Science
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• v.31 no.10
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• pp.898-904
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• 2021

Locusta migratoria is a widespread locust species in many parts of the world and is considered an alternative source for the production of protein for value-added ingredients. We previously identified putative antimicrobial peptides derived from L. migratoria through an in silico analysis of its transcriptome. However, its anti-inflammatory effect has not been studied. In this study, we investigated the anti-inflammatory activities of the antimicrobial peptide locustacin (KTHILSFFPSFLPLFLKK-NH₂) derived from L. migratoria on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Locustacin (50, 100, and 200 ㎍/ml) significantly reduced the production of nitric oxide (NO) in LPS-stimulated macrophages without any cytotoxicity. Locustacin also inhibited the mRNA and protein expression of pro-inflammatory mediators, such as inducible NO synthase and cyclooxygenase-2, in contrast to the presence of LPS alone. Locustacin decreased the release of LPS-induced pro-inflammatory cytokines, including interleukin (IL)-6 and IL-1β, and their gene expression in a dose-dependent manner. Furthermore, locustacin (100 and/or 200 ㎍/ml) inhibited phosphorylation levels of extracellular signal regulated kinase, p38, and c-Jun N-terminal kinase. Locustacin also suppressed the degradation of inhibitory kappa B alpha, which was considered to be an inhibitor of nuclear factor kappa B (NF-κB). Collectively, these results demonstrate that locustacin can exert anti-inflammatory effects through the inhibition of mitogen-activated protein kinase (MAPK) phosphorylation, activation of NF-κB, and downstream inflammatory mediators in LPS-stimulated macrophage cells.

• Journal of Life Science
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• v.31 no.11
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• pp.987-994
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• 2021

Our previous studies have reported that antimicrobial peptides (AMPs) derived from the larvae of white-spotted flower chafer (Protaetia brevitarsis seulensis) exert anti-inflammatory and neuroprotective activities. This study explored the anti-inflammatory effects of protaetiamycine 9 (CVLKKAYFLTNLKLRG-NH₂), a novel AMP, derived from P. b. seulensis against lipopolysaccharide (LPS)-mediated inflammatory response in RAW264.7 macrophage cells. Protaetiamycine 9 (25, 50, 75, and 100 ㎍/ml) did not cause cytotoxic effects against RAW264.7 cells. The RAW264.7 cells were pre-treated with various concentrations of protaetiamycine 9 (25-100 ㎍/ml) for 1 hr and then exposed to LPS (100 ng/ml) for 24 hr. Protaetiamycine 9 treatments decreased the LPS-induced secretion of inflammatory mediators, such as nitric oxide (NO), in a dose-dependent manner. Protaetiamycine 9 (25-100 ㎍/ml) effectively downregulated the LPS-induced increase in mRNA and the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), which are involved in the production of inflammatory mediators. Protaetiamycine 9 also suppressed the production and gene expression of pro-inflammatory cytokines, including interleukin (IL)-6 and IL-1β, compared to the presence of LPS alone. Furthermore, protaetiamycine 9 inhibited the degradation of inhibitory kappa B alpha (IκB-α) and the phosphorylation of mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. In conclusion, these results suggest that protaetiamycine 9 exhibits LPS-mediated inflammatory responses by blocking IκB-α degradation and MAPK phosphorylation.

• Journal of Life Science
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• v.32 no.11
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• pp.865-871
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• 2022

Tenebrio molitor larvae is well known as edible insect. Then, although it has been widely studied that Tenebrio molitor larvae has various bioactive functions such as antioxidant, anti-wrinkle, and anticancer. Nevertheless, antioxidant effects of Tenebrio molitor larvae water extract (TMH) has not been well described in Adult Retina Pigment Epithelial cell line (ARPE-19). In this study, we demonstrated that antioxidant effects of TMH against H₂O₂-induced oxidative stress in ARPE-19. Thus, we selected for our studies and performed a series of dose-response assay to determine the working concentration that lead to a consistent and high degree of cytotoxicity, which we defined as the level of H₂O₂ that killed 40% of the ARPE-19 cells. ARPE-19 cells were pre-treated with various concentrations of TMH (0.1 up to 2 mg/ml) before exposure to 300 µM H₂O₂. As we expected, TMH effectively prevented ARPE-19 cells from 300 µM H₂O₂-induced cell death in a dose-dependent manner. Furthermore, TMH inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Overall, the inhibitory effects of TMH on H₂O₂-induced apoptosis and oxidative stress were associated with the protection cleaved caspase-3, Bax, Bcl-2, and HO-1. The TMH suppressed H₂O₂-induced cell membrane leakage and oxidative stress in ARPE-19 cells. Thus, these results suggest that the TMH plays an important role in antioxidant effect in ARPE-19.

• Journal of Life Science
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• v.34 no.3
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• pp.191-198
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• 2024

To verify the nutritional value and safety of larvae fed with a soymilk residue-added food source, we compared and analyzed the nutritional components of and harmful substances in Tenebrio molitor larvae reared on wheat bran. Crude protein content based on dry weight was 1.2 times higher in the 10% soymilk residue-fed group (SR) (54.0%) than in the wheat bran-fed group (WB) (43.5%). Dietary fiber also tended to be 1.9 times more in the SR (4.9%) than the WB (2.5%). Among unsaturated fatty acids, the linoleic acid content was found to be 1.1-fold higher in the SR (32.9%) than in the WB (29.0%). Potassium, which was the most abundant among the macro minerals, was 1.1-fold more abundant in the WB (1,074.5 mg/100 g) than in the SR (1,014.0 mg/100 g). Among the micro minerals, zinc content was 1.2-fold higher in the SR (14.5 mg/100 g) than in the WB (11.9 mg/100 g). The results of the analysis of hazardous substances in the WB and SR revealed that the amount of heavy metals met the standards for heavy metals in edible insects, and food poisoning-inducing bacteria such as Escherichia coli and Salmonella spp. were not detected in all groups. These results indicate that mealworm larvae fed with 10% soymilk residue have abundant nutrients and are safe for intake. Thus, food sources with added soymilk residue has the potential to be used as feed ingredients.

• The Hankook-Saengyark Bo
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• no.260
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• pp.6-6
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• 2004

제1회 평창 약초축제 개최(당귀 최대 생산지 $\cdots$ 농가소득원 창출) - 누에 가공식품 웰빙시대 건강식 각광(양잠농가 소득증진에 '효자'노릇 '톡톡' - 장뇌 추출물로 기능성 화장품 개발