Browse > Article
http://dx.doi.org/10.5352/JLS.2022.32.11.865

Antioxidative Effects of Tenebrio molitor Larvae Extract Against Oxidative Stress in ARPE-19 Cells  

Bong Sun, Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Ra-Yeong, Choi (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Eu-Jin, Ban (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Joon Ha, Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
In-Woo, Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Minchul, Seo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Life Science / v.32, no.11, 2022 , pp. 865-871 More about this Journal
Abstract
Tenebrio molitor larvae is well known as edible insect. Then, although it has been widely studied that Tenebrio molitor larvae has various bioactive functions such as antioxidant, anti-wrinkle, and anticancer. Nevertheless, antioxidant effects of Tenebrio molitor larvae water extract (TMH) has not been well described in Adult Retina Pigment Epithelial cell line (ARPE-19). In this study, we demonstrated that antioxidant effects of TMH against H2O2-induced oxidative stress in ARPE-19. Thus, we selected for our studies and performed a series of dose-response assay to determine the working concentration that lead to a consistent and high degree of cytotoxicity, which we defined as the level of H2O2 that killed 40% of the ARPE-19 cells. ARPE-19 cells were pre-treated with various concentrations of TMH (0.1 up to 2 mg/ml) before exposure to 300 µM H2O2. As we expected, TMH effectively prevented ARPE-19 cells from 300 µM H2O2-induced cell death in a dose-dependent manner. Furthermore, TMH inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) such as extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Overall, the inhibitory effects of TMH on H2O2-induced apoptosis and oxidative stress were associated with the protection cleaved caspase-3, Bax, Bcl-2, and HO-1. The TMH suppressed H2O2-induced cell membrane leakage and oxidative stress in ARPE-19 cells. Thus, these results suggest that the TMH plays an important role in antioxidant effect in ARPE-19.
Keywords
AMD; antioxidant; apoptosis; ARPE-19 cells; Tenebrio molitor;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Wankun, X., Wenzhen, Y., Min, Z., Weiyan, Z., Huan, C., Wei, D., Lvzhen, H., Xu, Y. and Xiaoxin, L. 2011. Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro. Mol. Vis. 17, 3512.
2 Weng, S., Mao, L., Gong, Y., Sun, T. and Gu, Q. 2017. Role of quercetin in protecting ARPE‑19 cells against H2O2‑induced injury via nuclear factor erythroid 2 like 2 pathway activation and endoplasmic reticulum stress inhibition. Mol. Med. Rep. 16, 3461-3468.   DOI
3 Yating, Q., Yuan, Y., Wei, Z., Qing, G., Xingwei, W., Qiu, Q. and Lili, Y. 2015.Oxidized LDL induces apoptosis of human retinal pigment epithelium through activation of ERK-Bax/Bcl-2 signaling pathways. Cur. Eye Res. 40, 415-422.   DOI
4 Yigit, M., Gunes, A., Uguz, C., Yalcin, T. O., Tok, L., Oz, A. and Naziroglu, M. 2019. Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model. Int. J. Ophthalmol. 12, 930.
5 Zhang, Y., Ren, S., Liu, Y., Gao, K., Liu, Z. and Zhang, Z. 2017. Inhibition of starvation-triggered endoplasmic reticulum stress, autophagy, and apoptosis in ARPE-19 cells by taurine through modulating the expression of calpain-1 and calpain-2. Int. J. Mol. Sci. 18, 2146.   DOI
6 Zhu, C., Dong, Y., Liu, H., Ren, H. and Cui, Z. 2017. Hesperetin protects against H2O2-triggered oxidative damage via upregulation of the Keap1-Nrf2/HO-1 signal pathway in ARPE-19 cells. Biomed. Pharmacother. 88, 124-133.   DOI
7 Yu, J. M., Jang, J. Y., Kim, H. J., Cho, Y. H., Kim, D. I., Kwon, O. J., Cho, Y. J. and An, B. J. 2016. Antioxidant capacity and Raw 264.7 macrophage anti-inflammatory effect of the Tenebrio Molitor. Kor. J. Food Preserv. 23, 890-898.   DOI
8 Baek, M., Seo, M., Kim, M., Yun, E. Y. and Hwang, J. S. 2017. The antioxidant activities and hair-growth promotion effects of Tenebrio molitor larvae extracts (TMEs). J. Life Sci. 27, 1269-1275.   DOI
9 Chiang, Y. W., Su, C. H., Sun, H. Y., Chen, S. P., Chen, C. J., Chen, W. Y., Chang, C. C., Chen, C. M. and Kuan, Y. H. 2022. Bisphenol A induced apoptosis via oxidative stress generation involved Nrf2/HO-1 pathway and mitochondrial dependent pathways in human retinal pigment epithelium (ARPE-19) cells. Environ. Toxicol. 37, 131-141.   DOI
10 Costa, S., Pedro, S., Lourenco, H., Batista, I., Teixeira, B., Bandarra, N. M., Murta, D., Nunes, R. and Pires, C. 2020. Evaluation of Tenebrio molitor larvae as an alter- native food source. NFS Journal 21, 57-64.   DOI
11 Du, L., Chen, J. and Xing, Y. Q. 2017. Eupatilin prevents H2O2-induced oxidative stress and apoptosis in human retinal pigment epithelial cells. Biomed. Pharmacother. 85, 136-140.   DOI
12 Hong, J., Han, T. and Kim, Y. Y. 2020. Mealworm (Tenebrio molitor Larvae) as an alternative protein source for monogastric animal: a review. Animals 10, 2068.   DOI
13 Hytti, M., Piippo, N., Salminen, A., Honkakoski, P., Kaar- niranta, K. and Kauppinen, A. 2015. Quercetin alleviates 4-hydroxynonenal-induced cytotoxicity and inflammation in ARPE-19 cells. Exp. Eye Res. 132, 208-215.   DOI
14 Jajic, I., Popovic, A., Urosevic, M., Krstovic, S., Petrovic, M. and Guljas, D. 2019. Chemical composition of mealworm larvae (Tenebrio molitor) reared in Serbia. Con. Agr. 68, 23-27.   DOI
15 Johnson, J., Maher, P. and Hanneken, A. 2009.The flavonoid, eriodictyol, induces long-term protection in ARPE19 cells through its effects on Nrf2 activation and phase 2 gene expression. Investig. Ophthalmol. Vis. Sci. 50, 2398-2406.   DOI
16 Kauppinen, A., Niskanen, H., Suuronen, T., Kinnunen, K., Salminen, A. and Kaarniranta, K. 2012. Oxidative stress activates NLRP3 inflammasomes in ARPE-19 cells-implications for age-related macular degeneration (AMD). Immunol. Lett. 147, 29-33.   DOI
17 Kim, M. H., Chung, J., Yang, J. W., Chung, S. M., Kwag, N. H. and Yoo, J. S. 2003. Hydrogen peroxide-induced cell death in a human retinal pigment epithelial cell line, ARPE-19. Kor. J. Ophthalmol. 17, 19-28.   DOI
18 Koskela, A., Reinisalo, M., Petrovski, G., Sinha, D., Olmiere, C., Karjalainen, R. and Kaarniranta, K. 2016. Nutraceutical with resveratrol and omega-3 fatty acids induces autophagy in ARPE-19 cells. Nutrients 8, 284.   DOI
19 Li, Z., Dong, X., Liu, H., Chen, X., Shi, H., Fan, Y., Hou, D. and Zhang, X. 2013. Astaxanthin protects ARPE-19 cells from oxidative stress via upregulation of Nrf2-regulated phase II enzymes through activation of PI3K/Akt. Mol. Vis. 19, 1656.
20 Liang, R., Zhao, Q., Zhu, Q., He, X., Gao, M. and Wang, Y. 2021. Lycium barbarum polysaccharide protects ARPE19 cells against H2O2‑induced oxidative stress via the Nrf2/HO‑1 pathway. Mol. Med. Rep. 24, 1-8.
21 Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J., Tang, Y., Liu, L., He, J. and Huang, Z. 2017. Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Mol. Med. Rep. 16, 2069-2074.   DOI
22 Liu, L. and Wu, X. W. 2018. Nobiletin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage. Adv. Mol. Toxicol. 32, e22052.
23 Quan, J. H., Gao, F. F., Ismail, H. A. H. A., Yuk, J. M., Cha, G. H., Chu, J. Q. and Lee, Y. H. 2020. Silver nanoparticle-induced apoptosis in ARPE-19 cells is inhibited by Toxoplasma gondii pre-infection through suppression of NOX4-dependent ROS generation. Int. J. Nanomed. 15, 3695.   DOI
24 Ravzanaadii, N., Kim, S.H., Choi, W. H., Hong, S. J. and Kim, N. J. 2012. Nutritional value of mealworm, Tenebrio molitor as food source. Int. J. Indust. Entomol. 25, 93-98.   DOI