• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.181-184
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• 1987

The cello·tape anal swab specimens from 936 primary school children in Seoul, 308 primary school children in a rural area and 92 children in Nanjido, were examined for Enterobius vermicularis eggs. Out of 936 specimens from a primary school in Seoul, E. vermicularis eggs were detected in 114 specimens, the egg detection rate being 12.2%. In 308 specimens from a primary school in a rural area,77 (25.0%) were positive for 5. vermicularis eggs. Among 92 specimens from Nanjido children in Seoul, egg Positive rate of E. vermicularis was 28.3%. The egg detection rate of primary school children in Seoul was significantly lower than that of primary school children in a rural area and of Nanjido children. The egg detection rate in boys was lower than that of girls. As the age of children increased, the egg detection rate decreased.

• Childhood Kidney Diseases
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• v.2 no.1
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• pp.1-8
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• 1998

Purpose : To evaluate the diagnostic value of dipstick urinalysis($Multistix^{(R)}$) as a screening test for urinary tract infection(UTI) in acute febrile children. Methods : We compared dipstick uinalysis (leukocyte estease(LE) and nitrite) with microscopic urinalysis (WBC and bacteria) and urine culture in 80 children with UTI and 605 children without UTI, who were admitted due to acute fever at EWHA Womans University Hospital between Oct. 1994 and Feb. 1996. Results : 1) In dipstick urinalysis, LE and nitrite were positive in 38.7%, and 13.8% of 80 children with UTI and in 2.1%. and 1.2% of 605 children without UTI. 2) In microscopic urinalysis, WBC and bacteria were positive in 31.2%, and 17.5% of 80 children with UTI and in 4.1%, and 0.6% of 605 children without UTI. 3) Positive rate of LE on dipstick urinalysis was 38.7%, which was significantly higher than 31.5% of WBC on microscopic urinalysis (P<0.05). Positive rate of nitrite was not significantly different to that of bacteria. 4) Sensitivity, specificity, positive predictive value, negative predictive value of dipstick urinalysis were 43.7%, 98.3%, 63.6%, 92.9% which were not significant different to 40.0%, 99.0%, 54.0%, 92.3% of microscopic urinalysis. The sensitivity of combined dipstick and microscopic urinalysis were still low(50%). 5) Additional positive rate of microscopic urinalysis to negative dipstick urinalysis was 6.3%(5/80) in 80 children with UTI. Conclusion : The sensitivity of dipstick urinalysis was low and additional value of microscopic urinalysis was minimal in febrile UTI. So urine culture is mandatory in acute febrile children even though dipstick and microscopic urinalysis are negative.

• Korean Journal of Agricultural Science
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• v.4 no.1
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• pp.66-74
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• 1977

To investigate the condition of infectious rate of fascioliasis in the farm animals. This survey was carried out, by the interdermal reaction from different animals, ages and pestures in Daejeon Suburbs and Cattle market and Abattoir. The average positive rate of infectious reaction were 31.0% in Korean Cattle, 38,7% in dairy Cattle, 33.8% in Korea native Goats and 26.9% in dairy Goat. The positive rate was higher at the age of 2~6 that compares with at age of yearling. From survey on the river side area, valley and wet area, the rate of positive was occured higher, than from plain, high land and in case of animals have not been grazing. And the detective rate of liver fluke among the positive reaction was occured 92.5% in Korean Cattle and 94.7% in Korea native Goat.

• Journal of Yeungnam Medical Science
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• v.4 no.1
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• pp.75-80
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• 1987

The positive rate for serum anti-HBs was analized among 424 of HB vaccinated and 2035 of non-vaccinated cases at the Yeungnam University Hospital, Computed Automated Med-screening Center. Most of them from Kyungbook province and they had the last HB vaccination in the periods of 3 to 42months prior to this study. The followings were obtained. 1. The positive rates for serum HBsAg were 0.7% in the vaccinees, 9.6% in the non-vaccinated and 8.0% in the whole cases. 2. The positive rates for serum anti-HBs were 66.3% in the vaccinees, 47.9% in the non-vaccinated and 51.0% in the whole cases. 3. The positive rates for serum anti-HBs were 51.4% in the cases with one time of vaccination and 68.6% in the cases with two times of vaccination. on the vasis of these findings the positive rate for serum anti-HBs among the vaccinees was significantly higher than thau of non-vaccinated(P<0.05). The positive rate for seaum anti-HBs shortly after vaccination was higher than that of present our study which was made relatively long period after vaccination. As the reason a natural decrease of the titers of the serum anti-HBs can be postulated as one of the contributing factors for the discrepancy. In order to keep the serum antibody of perfect protectivity against HBV, it may be better to check the serum anti-HBs just after vaccination, follow up and take booster injection when it is needed.

• Childhood Kidney Diseases
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• v.3 no.2
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• pp.117-122
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• 1999

Purpose : We studied the correlation between urinalysis and radiologic findings in infants and children with urinary tract infection. Method : Urine Dipstick test and unstained urine microscopic examination were carried out in 56 infants and children who were hospitallized with the diagnosis of urinary infection by pocitive urine culture at Seoul Adventist from September 1996 through August 1998. Urine was collected by midsream, catheter, urine bag after cleansing or bladder puncture. Renal sonography and $^{99m}Tc-DMSA$ renal scan or voiding cystoureterography were studied. Results : 1)In dipstick analysis, leukocyte esterase(LE) were positive in 30 cases of 56 patients(54%) and Nitrite were positive in 20 cases of 56 patients(36%) and LE or Nitrite were positive in 38 cases of 56 children with UTI(68%). 2)In microscopic analysis, WBC were positive in 38 cases of patients(68%) and bacteria were positive in 23 cases of 56 patients(41%) and WBC or bacteria were positive in 41 cases of 56 children with UTI(71%). 3)Ten cases of 56 UTI patients(18%) showed negative finding in Dipstick and microscopic analysis. 4)There was no significant difference in positive rate of dipstick(71% vs 66%) and microscopia analysis(83% vs 66%) between two group with or without renal scar on $^{99m}Tc-DMSA$ scan (P=0.117). 5)There was no significnt difference in positive rate of dipstick(100% vs 91%) and microscopic analysis(100% vs 100%) between two groups with or without vesicoureteral reflux on VCUG. Conclusion : There was no specific relationships between the abnormal urinalysis and the abnormal findings on $^{99m}Tc-DMSA$ scan or VCUG.

• Parasites, Hosts and Diseases
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• v.24 no.2
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• pp.205-208
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• 1986

A total of 860 cellotape anal swab specimens from 326 school children of urban area, 243 of rural farm village and 291 of rural fishing village was submitted for Enterobius vermicularis egg detection from September through November, 1985. Each specimen was collected in the morning. Among the total 860 children's submitting specimens, 316 (36.7%) were positive for E. vermicularis eggs. Of the 326 children in the urban area, E. vermicularis eggs were detected in the 45 (13.8%). This detection rate was lower significantly as compared with 143 (55.6%) of 243 children in the farm village or 136 (46.7%) of 291 children in the fishing village. The egg detection rate in the male children was lower remarkably than that in the female children.

• Tuberculosis and Respiratory Diseases
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• v.40 no.1
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• pp.23-28
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• 1993

Background: Since an important component of carcinogenesis is unregulated growth, many investigators have reported the methods to detect cell proliferation in tissues including PCNA. PCNA is a 36 Kd intranuclear polypeptide and plays a critical role in cell proliferation. Thus progressive dysregulation of proliferation during carcinogenesis can be directly visualized in the paraffin embedded tissue using immunohistochemistry for PCNA which has an advantage of simplicity and maintenance of tissue architecture. The heterogeneity of PCNA expression is known to be related with proliferating fraction, histologic grade, DNA ploidy, and susceptibility of anticancer drugs, etc. We analyzed the biologic significance of the expression of PCNA in lung cancer tissues. Method: 43 lung cancer tissues, which were resected by surgery and were embedded in paraffin, were stained immunohistochemically by one hour MicroProbe System and the results were corelated with cell type, stage, site and survival. Result: 1) Suamous cell type showed high positivity (89%) than in adenocarcinoma (54%). 2) No significant difference related to tumor stage was noticed. 3) No significant difference between primary site and metastatic site was noticed. 4) No significant difference in 12-month survival between positive group and negative group was noticed. Conclusion: From this study, we concluded that imunohistochemistry for PCNA expression of routinely processed tissue is a simple technique for the assessment of proliferation in non-small cell lung cancer. Whether the labelling index has an independent prognostic value and deserves special attention in pathobiological evaluation in lung cancer remains to be investigated from large series with longer follow-up and to be correlated with multiple biological markers.

• Journal of Yeungnam Medical Science
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• v.5 no.2
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• pp.53-58
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• 1988

KOH examination is a simple, rapid and diagnostic procedure to confirm dermatophytic infections. It is important to select a proper examination site of the lesion. To determinate the proper examination site of the lesion, mycologic studies were done with multiple specimens collected from the center, margin and out of margin of the ring-shaped dermatophytotic skin lesion on the 58 patients. The results were as follows. Positive rate of KOH wet smear was 94.8% at the center and 100% at the margin of the lesions, 22.4% at the 1cm and 5.2% at the 2cm out of the lesions. The more hyphae were found in the lesion, the more hyphae were found out of the lesion. Culture was done on the Sabouraud's glucose agar from the highest KOH positive area and the positive culture was 48 strains(82.8%) of 58 patients. These findings suggested that the ring-shaped active margin was the best site to examine mycologic studies.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.167-172
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• 1998

한국 및 일본을 포함한 동아시아 지역에 벡터 매개성 바이러스에 의한 소 질병으로 인하여 경제적 손실이 몇년을 주기로 극심하게 나타나고 있다. 그러나 본 질병의 정확한 역학에 대하여는 아직까지 확실한 연구가 되어 있지 않은 상태이다. 본 연구에서는 벡터 매개성 질병에 대한 역학을 좀더 명확하게 밝히기 위하여, 일본 열도중 가장 멀리 떨어져 있으며, 아열대 지역에 속하는 오끼나와 섬에서 사육되고 있으면서 백신접종을 받지 않은 소(Japanese black cattle)를 대상으로 하여 1988년도부터 1992년도에 걸쳐 벡터 매개성 바이러스군에 대한 중화항체가를 측정하고 분석하였다. 소의 유행성 바이러스(bovine ephemeral virus)에 대한 항체 양성율은 연도 및 계절별로 크게 변화를 보였고, Ibaraki virus에 대한 항체양성율은 그 어느 다른 계절보다도 5월에 높은 항체가를 보였으며, Akabane virus에 대한 항체 양성율은 이바라키 바이러스에 대한 항체 양성율과 유사한 수준으로 나타났다. 한편 Chuzan virus에 대한 항체양성율은 계절적인 변화가 심하지 않은 것으로 나타났다.

• Biomedical Science Letters
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• v.2 no.2
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• pp.267-274
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• 1996

This study was performed to observe the present status of intestinal parasitic infections among the children in Iksan, Jeonbuk, Korea. During the period from February to June 1996, total 510 fecal samples(male 276, female 234) were collected and examined by formalin-ether sedimentation technique and trichrome stain for intestinal helminths and protozoan cysts. Of 510 samples examined, 62(12.2%) were positive for intestinal parasites. Helminth egg positives were 3(0.6%), and protozoan cyst positives were 59(11.6%). The female group showed higher positive rate(13.2%) than males(11.2%), and the highest positive rates were observed in 7 age group(17.5%), and lowest positive rates were 4 age group(9.5%). 3 species of helminths egg and 6 species of protozoan cyst were detected; Metagonimus yokogawai 0.4%, Enterobius vermicularis 0.2%, Trichuris trichiura 0.2%, Endolimax nana 4.1%, Entamoeba coli 3.9%, Ciardia lamblia 2.0%, Entamoeba histolytica 1.4%, Iodamoeba butschilii 0.8%, and Chilomastix mesnili 0.4%, respectively. In 3 helminth egg positive cases, single infection was 2(66.7%), and double infection 1(33.3%), among 59 protozoan cyst positives, single infection was 54(91.5%), and double infection was 5(8.5%). The highest positive rate of protozoan cyst was 7 age group(14.3), and lowest positive rate was 4 age group(9.5%).