• Journal of Food Hygiene and Safety
• /
• v.33 no.5
• /
• pp.399-403
• /
• 2018

In this study, safety investigations on harmful microorganisms and mycotoxins were conducted on honeybee drone pupae as a new food material, which is rich in nutrients and capable of being mass produced in apiaries. The honeybee drone pupae produced in apiaries were collected from three different regions in Korea and frozen immediately. Subsequently, the samples were subjected to freeze-drying. According to the Korean Food Code test method, coliforms, Salmonella species, Staphylococcus aureus, and enterohemorrhagic Escherichia coli were not detected in 280 honeybee drone pupas. In addition, mycotoxins, aflatoxin $B_1$, ochratoxin A, deoxynivalenol, and zearalenone were not detected. Therefore, it is proposed that the honeybee drone pupae collected from the beehives and immediately frozen as safe from harmful microorganisms and mycotoxins and can be used as a food material.

• Journal of Apiculture
• /
• v.32 no.3
• /
• pp.269-273
• /
• 2017

We investigated whether purified bee venom increases the enzymatic activity of the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). ADH and ALDH assay were tested by in vitro kits. The purified bee venom was assayed by ultra performance liquid chromatography, The contents of melittin, apamin and phospholipase A2, as main component of purified bee venom, were 63.9%, 2.3%, and 10.9%, respectively. The ADH and ALDH acitivity of purified bee venom(at 1mg/ml) were $88.6{\pm}7.34%$ and $94.6{\pm}0.57%$, respectively compared with positive control at 2mg/ml. These results showed that purified bee venom induces the activity of ADH and ALDH which reduce the aldehyde concentration in the blood, suggesting the possibility of purified bee venom as resource of medicine or functional beverage for hangover relieving.

• Journal of Apiculture
• /
• v.34 no.1
• /
• pp.15-26
• /
• 2019

Tracheal mite (Acarapis woodi) is an internal parasite that is parasitic on the bronchus of adult bees and sucks fluid from the trachea. Since its first report by Rennie, it has been spread throughout Europe and in some Asian regions, with adjacent Japan and China reported in 2011 and 2012, respectively. Korea detected specific genes of A. woodi in 2015, but only one of 99 samples has been identified and the being of A. woodi has not been confirmed. In this study, we established a specific nested PCR method to confirm for detecting low-copy number of A. woodi-specific gene in bee samples. As a result, A. woodi-specific COI gene was amplified in 15 of 23 samples, and they were judged positive by melting point analysis and sequencing analysis. Although we could not observe the existence of the mites in bees, our results suggest that tracheal mit might exist in nature.

• Journal of Sericultural and Entomological Science
• /
• v.51 no.1
• /
• pp.56-62
• /
• 2013

We bred new mulberry cultivar through local adaptability test, which was registered as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Chuncheon and Jinju) for four years since 2003. This is tetraploidy variety belonging to (Morus Lhou(Ser.) Koidz.) made by colchicine treatment on growing point of winter buds. Daejappong was high yielding in productivity by 3% compared to control cultivar "Chungilppong" for two years.The sugar contents of Daejappong was higher than that of control cultivar 'Chungilppong(Morus alba L.)'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Journal of Sericultural and Entomological Science
• /
• v.52 no.1
• /
• pp.64-72
• /
• 2014

We bred a mulberry cultivar named Suhyang through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test was carried out at four places (Suwon, Kongju, Wanju and Jinju) for six years from 2007 to 2012. This is belonging to (Morus alba L.) crossed between Josaenghongpisang (Morus alba L.) and Kaeryang (Morus alba L.) among mulberry genetic resources preserved in Suwon. Suhyang was high yielding cultivar in fruit productivity by 12% compared to control cultivar 'Chungil (Morus alba L.)' for four years. Although Suhyang was higher in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Journal of Sericultural and Entomological Science
• /
• v.51 no.2
• /
• pp.130-136
• /
• 2013

We bred a mulberry cultivar named Shimheungppong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Wanju, and Jinju) for six years from 2004. This is tetraploidy variety belonging to (Morus alba L.) selected among mulberry genetic resources preserved in Suwon. Shimheungppong was high yielding cultivar in fruit productivity by 5% compared to control cultivar 'Chungilppong(Morus alba L.)' for four years. Although Shimheungppong was a little lower in sugar content of mulberry fruits, it was bigger than that of Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
• /
• 2000.11a
• /
• pp.139-139
• /
• 2000

종이는 제조 후 시간의 경과에 따라 노화가 야기되기 시작하며 이에 수반되는 현상으로서 종이의 기계적 강도 손실 및 종이의 색 변화를 들 수 있다. 종이의 노화 현상은 주로 빛, 열, 대기 오염물질, 미생물, 곤충 및 화학약품 등의 외부 인자들에 의해 종이 내에서의 가수 분해 또는 산화작용을 발생시키며 이는 종이의 폭넓은 이용올 제한하는 중요한 원인이 되고 있다. 종이의 노화기작은 주로 산 가수분해 및 산화작용 그리고 가교결합 둥으로 해석되고 있다. 이는 종이의 주 구성요소인 셀룰로오스의 수산기가 반웅하여 카르보닐기를 형성하면 서 저분자화 되거나 산소에 의해 산화되면서 저분자화 되어 종이의 강도적 손실이 일어난다 고 보고되고 있으며 종이의 황색화(Yellowing) 현상은 주원인이 종이에 잔존하고 있는 리그 년이 빛과 열에 의해 반응하여 산화됨으로써 야기된다고 설명되고 있다. 즉, 열이나 자외선 및 가시광션의 조사로 인한 셀룰로오스 및 기타 종이 구성물의 산화에 의해 종이가 퇴색되 거나 강도가 저하되는 현상이 일어나게 된다. 특히 이러한 노화 거동은 상온의 경우에서는 펄프와 종이의 황색화가 천천히 일어나지만 옹도가 점차 올라갈수록 그 속도는 빨라진다. 종이가 노화되면서 일어나는 산화반용은 주로 대기 중의 산소와 접촉하기 쉬운 표변에서부 터 발생하기 쉽다. 열처리를 통해 표면에서의 산화 작용은 촉진되고 종이의 구성원소의 결 합에 화학적 변화가 야기된다. 이를 분석하기 위해서 모든 원소가 독특한 결합에너지를 가 지고 있다는 것에 착안 시료 표면에 특정 x-선 및 전자빔을 입사하여 방출하는 광전자의 에너지를 측정함으로써 시료 표면의 조성 및 화학적인 결합상태를 알 수 있는 ESCA ( (Electron Spectroscopy for Chemical Analysis)를 이용하였다 .. ESCA는 주로 표면 원소의 규 명 및 정량분석과 화학결합 상태의 정성, 정량 분석, 깊이에 따른 원소의 농도 분포 분석, 고분자화합물의 특성 조사, 표면 원소의 화학결합에 따른 전자상태 연구 둥에 활용되 고 있 다. 즉, 종이가 노화되면서 원소들 사이에 변화되는 결합을 이러한 에너지 분석에 의해 원소 정성분석 또는 정량분석을 하고자 하였으며, 이를 분석하여 열처리 시 종이 표면에서 일어 나는 변화를 구명하고자 하였다. 이에 따라 본 연구에서는 종이의 노화를 가속화시키는 빛, 대기오염물질, 및 기타 다른 인 자들은 배제하고 열 만을 가해 노화의 진행속도를 높인 후, 노화 진행 시 종이 표변에 일어 나는 산화작용 및 가수분해를 표면 분석 장치인 ESCA를 이용하여 종이의 주 구성원소인 탄소와 산소가 열처리 시 변하는 에너지를 측정하였다. 또한 카르복실기 정량과 종이의 pH 측정 및 X -ray Diffractometer를 이용하여 결정화도를 측정하였다. 본 연구의 결과, 시간의 경과에 따라서 탄소의 결합에너지는 분포가 C-H에서 COO-, 또는 C=O로 달라짐으로써 종 이가 산화되고 있다는 것을 알 수 있었다. 또한 이 결합에너지 분포의 변화가 펄프의 종류 에 따라서 다르게 이동함으로써 제조된 시트의 표면 산화반응이 서로 다르게 일어나고 있음 을 알 수 있었으며, 이는 사용한 펄프의 화학 조성분의 차이에 기인한 것이라 사료된다.

• Journal of Korean Society of Transportation
• /
• v.35 no.1
• /
• pp.63-78
• /
• 2017

The purpose of this study is to determine the optimal aggregation interval to increase the reliability when estimating representative value of individual vehicle travel time collected by DSRC equipment in interrupted traffic flow section in National Highway. For this, we use the bimodal asymmetric distribution data, which is the distribution of the most representative individual vehicle travel time collected in the interrupted traffic flow section, and estimate the MSE(Mean Square Error) according to the variation of the aggregation interval of individual vehicle travel time, and determine the optimal aggregation interval. The estimation equation for the MSE estimation utilizes the maximum estimation error equation of t-distribution that can be used in asymmetric distribution. For the analysis of optimal aggregation interval size, the aggregation interval size of individual vehicle travel time was only 3 minutes or more apart from the aggregation interval size of 1-2 minutes in which the collection of data was normally lost due to the signal stop in the interrupted traffic flow section. The aggregation interval that causes the missing part in the data collection causes another error in the missing data correction process and is excluded. As a result, the optimal aggregation interval for the minimum MSE was 3~5 minutes. Considering both the efficiency of the system operation and the improvement of the reliability of calculation of the travel time, it is effective to operate the basic aggregation interval as 5 minutes as usual and to reduce the aggregation interval to 3 minutes in case of congestion.

• Korean journal of applied entomology
• /
• v.56 no.4
• /
• pp.331-338
• /
• 2017

In the present study, we investigated the effects of two herbicide-resistant genetically modified rice (GM rice) varieties, Milyang 204 and Iksan 483, recently developed in Korea on non-target insects and a spider. No difference in host preferences of the English grain aphid Sitobion avenae and the brown planthopper Nilaparvata lugens were observed between GM rice and non-GM rice. Wolf spider Pirata subpiraticus, feeding on N. lugens reared on GM rice or non-GM rice, revealed no significant difference in body weight. P. subpiraticus, fed with N. lugens reared on Milyang 204, showed survival rates similar to that in P. subpiraticus fed with N. lugens reared on non-GM rice. However, P. subpiraticus feeding on N. lugens reared on Iksan 483 demonstrated significantly lower survival rates than that in P. subpiraticus feeding on N. lugens reared on Milyang 204 or non-GM rice. In addition, when larvae of the western honeybee Apis mellifera were supplied with Iksan 483 pollen, a significantly longer pupal period occurred, as compared with that of A. mellifera supplied with pollen of Milyang 204 or non-GM rice. As GM rice has negative effects on P. subpiraticus, which is an important predator in agricultural ecosystems, and on A. mellifera, which plays important roles in pollination and honey production, additional studies on risk assessment of GM rice should be conducted before releasing newly developed herbicide-resistant GM rice to the agricultural environment.

• Journal of Sericultural and Entomological Science
• /
• v.52 no.2
• /
• pp.147-154
• /
• 2014

We bred a mulberry cultivar named Palcheong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four places (Suwon, Kongju, Wanju, and Jinju) for seven years from 2007. This is belonging to (Morus Lhou(Ser.) Koidz.) selected among mulberry genetic resources preserved in Suwon. Its sex expression was hermaphrodite, which can be used for the production of seedling for fruit production by artificial crossing as pollen parent Palchung showed lower yielding in fruit productivity by 16% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Palchung was higher in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.