• 한국동물위생학회지
• /
• 제37권1호
• /
• pp.11-18
• /
• 2014

Porcine reproductive and respiratory syndrome (PRRS) causes a significant economic loss in the swine industry not only in Korea but also all over the world. Andong and Hapcheon region were selected for Area Regional Control (ARC) programme to reduce the shedding of PRRS virus (PRRSV) and decrease PRRS outbreaks. Before conducting the PRRS ARC, sera of pigs were tested for both antibody using ELISA and antigen using RT-PCR, then phylogenetic classifications was analysed. Pigs of 138/275 (50.2%) in Andong and 352/425 (82.8%) in Hapcheon were seropositive. Also, the RT-PCR results revealed that 27 heads (8.2%) in Andong, 112 heads (22.0%) in Hapcheon were positive for PRRSV antigen. PRRSVs were mainly detected between the ages of 40 to 60 days. PRRSV ORF5 regions were used to determine genetic clusters based on previous report. All PRRSV type I detected in both Andong and Hapcheon were classified as Cluster I. The PRRSV type II isolates in Andong were assorted to Cluster II, whereas the PRRSV type II isolates in Hapcheon were the viruses were unassembled into any cluster except one identified to Cluster III. Phylogenetic analysis indicated that new clusters of PRRSVs type II were prevalent in Hapcheon.

• 대한수의학회지
• /
• 제45권1호
• /
• pp.55-61
• /
• 2005

Serum samples of 1,175 pigs from 148 Korean swine farms not using Mycoplasma hyopneumoniae (M. hyo) vaccines were collected for seroepidemiological study of M. hyo infection by indirect ELISA method. Informations of each farm were provided about province where the farm was located and season when blood samples were collected. Then, the selected farms were divided into farm units which had 5 serum samples according to production stages : sow, suckling piglet (<30 days old), nursery pig (30-70 days old), and growing pig (>70 days old). Seroprevalence of M. hyo infection according to production stages, province, and season was investigated by using ELISA-positve rate of the selected samples for each study. This study showed that 85.34% (78.94-91.78%, 95% CI) of farms were positive to M. hyo infection and 34.81% (32.09-37.53%, 95% CI) among pigs were sero-positive to M. hyo infection in Korean swine farms. In the study of seroprevalence by production stage, most farms had sows and growing pigs which were sero-positive to M. hyo infection (sow: 83.05%, growing pigs: 87.72%) and most pigs seemed to be naturally infected by M. hyo at 8-10 weeks of age. Also, M. hyo infection showed seasonal pattern that most pigs were infected in late fall to early winter. However, in the study of seroprevalence by province, there was no significant correlation between province and M. hyo sero-positive rate.

• 한국동물위생학회지
• /
• 제37권4호
• /
• pp.233-240
• /
• 2014

Salmonella spp. are one of the most common bacteria that causes heavy losses in swine industry and have implications for public health. In this study, the correlation between Salmonella seroprev-alence on farms and the isolation rate from slaughtered pigs was analyzed and the antimicrobial resistance of the isolated Salmonella spp. was investigated. A total of 3,001 serum samples for ELISA were collected from 17 farms during two consecutive years (2012-2013). The mean values of ELISA OD% for each 8 age groups were as follows; gilt 27.83 (n=472), sow 23.75 (n=367), 150 days (d) of pig 16.53 (n=278), 130 d 11.87 (n=366), 100 d 9.46 (n=378), 20 d 9.17 (n=394), 70 d 6.56 (n=382), 40 d 3.72 (n=364). From July 2013 to January 2014, a total of 53 (8.0%) Salmonella strains were isolated from 665 slaughtered pigs shipped from those 17 farms. The mean values of ELISA OD% for each age groups serum samples that were collected in the second half of 2013 showed a positive correlation at 100 d (0.61, P<0.05), 130 d (0.45, P<0.1) with the isolation rate of Salmonella spp. in the slaughtered pigs. All the isolates were identified by a real-time PCR and tested for antimicrobial susceptibility. As a result, the predominant serovar was S. Typhimurium (52.8%) and there were 15 strains showing their own antimicrobial resistance pattern. All strains were susceptible to amoxicillin, cefepime, ciprofloxacin and some of them were resistant to streptomycin and tetracycline (60%), ampicillin (53.3%), chloramphenicol (33.3%), respectively.

• 한국축산시설환경학회지
• /
• 제2권1호
• /
• pp.1-11
• /
• 1996

본 연구는 사육, 급사, 급수, 번식, 건강 관리, 환기 및 열 관리, 자료 분석 등에 대한 기계화 및 자동화의 현황과 수준을 분석 평가하고, 이를 토대로 양돈 시설의 최적화 생산 시스템 모델 개발의 연구 방향을 설정하기 위하여 수행되었으며 앞으로의 연구 방향을 다음과 같이 설정하였다. 1) 종모돈, 종빈돈, 분만돈은 단사식으로 사육하고, 자돈, 육성돈, 비육돈은 군사식으로 사육하는 것과 돈방 배열은 복열식으로 하는 것을 기준으로 한국형 환경 제어용 스라트 무창돈사를 개발한다. 2) 한국형 환경 제어용 스라트 무창돈사에 적합한 환경 제어 시스템과 분뇨 처리 시스템을 개발한다. 3) 전자식 개체식별장치를 개발한다. 4) 전자식 개체식별장치와 컴퓨터를 이용한 자동 개체 습식 급사 시스템을 개발한다. 5) 번식돈을 위한 전자식 개체식별장치에 의한 개체 관리 시스템을 개발한다. 6) 건강 관리 및 자료 분석 등을 종합 관리할 수 있는 전문가 시스템을 개발한다.

• 한국농공학회논문집
• /
• 제64권1호
• /
• pp.1-13
• /
• 2022

In Korea, attention is being paid to the use of renewable energy in the livestock industry, and Ground Source Heat Pump (GSHP), which is advantageous for temperature control, is considered as one of the ways to reduce the use of fossil fuels. But GSHP is expensive to install, which proper capacity calculation is required. GSHP capacity is related to its maximum energy load. Energy loads are affected by climate characteristics and time, so dynamic analysis is required. In this study, the optimal capacity of GSHP was calculated by calculating the heating and cooling load of pig farms using BES (Building Energy Simulation) and economic analysis was performed. After designing the inside of the pig house using TRNSYS, one of the commercial programs of the BES technique, the energy load was calculated based on meteorological data. Through the calculated energy load, three heating devices and GSHP used in pig farms were analyzed for economic feasibility. As a result, GSHP's total cost of ownership was the cheapest, but the installation cost was the highest. In order to reduce the initial cost of GSHP, the capacity of GSHP was divided, and a scenario was created in which some of it was used as an auxiliary heating device, and economic analysis was conducted. In this study, a method to calculate the proper capacity of GSHP through dynamic energy analysis was proposed, and it can be used as data necessary to expand the spread of GSHP.

• 한국동물위생학회지
• /
• 제44권4호
• /
• pp.315-320
• /
• 2021

To analyze prevalence of mycotoxins, a total of 74 feedstuff samples were collected from silos (n=37) and hoppers (n=37) in nine pig farms. Six mycotoxins were tested with commercialized ELISA kits. All samples were contaminated with four or more mycotoxins. Zearalenone was detected in all of the tested samples. Ochratoxin, deoxynivalenol and H-2/HT-2 toxin were detected in more than 90% of the samples. And also, fumonisin was positive in 89.2% of the samples from the silos, 75.2% from the hoppers, respectively. On the other hand, aflatoxin was detected in about 40% of the samples. When the behavior of lactating sows was observed, possible mycotoxicosis was suspected. It was confirmed that their feedstuffs were contaminated with high levels of mycotoxins such as ochratoxin and T-2/HT-2 toxin. After cleaning the feedline, the clinical symptoms in sows suspected with mycotoxicosis were disappeared. Although mycotoxin concentration in most of the feedstuffs was below the acceptance level, these data indicate that what are required is more monitoring and continuous management for mycotoxins in pig feedstuffs.

• 한국동물위생학회지
• /
• 제44권4호
• /
• pp.217-225
• /
• 2021

We investigated the effect of temperature and stock density on the collection efficiency of oral fluid in the pig farm in Korea. Three pig farms with similar breeding environmental conditions were selected and four pens of each farm (total 12 pens) were tested for the collection efficiency of oral fluid from pigs. Collection rate was considered as significant when oral fluid was collected from 70% of pigs within a pen. In the case of growing pigs, when internal temperature of pig barn increased by one designated degree (5℃), the oral fluid collection rate significantly decreased by 24.7% (P<0.05). The collection rate of oral fluid also decreased by 7.1% (P<0.05) as the density rate increase by one designated degree (12.5%). It was estimated that the collection efficiency of oral fluid decreased when the internal temperature of pig barn was 30℃ or higher, or barn density is higher 25% or high. On the other hand, in the case of stall-housing sows, unlike growing pigs, there was no significant differences according to the temperature, so oral fluid collection was considered to be efficient even in hot season.

• 한국동물위생학회지
• /
• 제46권4호
• /
• pp.325-334
• /
• 2023

Edema disease (ED) in pigs is enterotoxemia caused by Shiga toxin type 2e (Stx2e)-producing Escherichia coli (STEC) and frequently occurs in young piglets. Therefore, ED causes enormous economic losses in pig farms. In this study, a modified Stx2e (mStx2e) antigen was expressed and purified using commercial E. coli expression system. Monoclonal antibody was serviced by Ynto Ab Inc., using Phage Display Technique. Anti-Stx2e antibodies in piglets were measured by indirect ELISA using mStx2e antigens. Naive Stx2e in piglets were detected by Sandwich ELISA using Stx2e-monoclonal antibodies and commercial Stx2e-polyclonal antibodies. Among 3,480 piglets, anti-Stx2e antibodies were observed in 2,573 piglets. The 49.4% among 830 piglet serum samples possessed 0.625 ㎍/mL or more of Stx2e proteins. The 18.3% of 830 sera had 0.313 ㎍/mL of Stx2e proteins. The 32.3% of 830 samples held 0.156 ㎍/mL or less of Stx2e proteins. These results show that indirect ELISA using mStx2e antigen and Sandwich ELISA using Stx2e-monoclonal and polyclonal antibodies can be useful to detect ED in piglets.

• 한국환경농학회지
• /
• 제42권4호
• /
• pp.269-278
• /
• 2023

Antibiotics either kill or inhibit the growth of bacteria. However, antibiotic-resistant bacteria are difficult to treat with antibiotics. Infections caused by such bacteria often lead to severe diseases. Antibiotic resistance genes (ARG) can be horizontally transmitted across different bacterial species, necessitating a comprehensive understanding of how ARGs spread across various environments. In this study, we analyzed the plasmid sequences of 33 extended-spectrum beta-lactamases (ESBL) producing Escherichia coli isolated from pigs, farms, and their owners. We conducted an antibiotic susceptibility test (AST) with aztreonam and seven other antibiotics, as well as whole genome sequencing (WGS) of the strains using MinION. Our results demonstrated that the plasmids that did not harbor ARGs were mostly non-conjugative, whereas the plasmids that harbored ARGs were conjugative. The arrangement of these ARGs exhibited a pattern of organization featuring a series of ARG cassettes, some of which were identical across the isolates collected from different sources. Therefore, this study suggests that the sets of ARG cassettes on plasmids were mostly shared between pigs and their owners. Hence, enhanced surveillance of ARG should be implemented in farm environments to proactively mitigate the risk of antibiotic-resistant bacterial infections.

• 한국초지조사료학회지
• /
• 제26권1호
• /
• pp.15-24
• /
• 2006

본 연구는 제주도의 서부지역 돼지사육농가에서 생산되고 있는 돈분액비의 비료가치 및 전기전도도나 건물함량과 성분간 상관관계를 구하여 간편한 비료가치 구명방법을 찾고자 수행되었다. 돈분액비의 시료는 118개 농가에서 수집되었고 건물수준을 기준으로 4개의 형태로 분류하였다. 전기전도도 (EC), 건물함량(DM), 암모니아성질소$(NH_4-N)$과 여러 가지 무기물을 분석하였고 이들 성분간에 상관성이 조사되었다. 118개 양돈농가 중 92개 농가는 액비의 건물함량이 3% 미만이었으며, $3{\sim}6%$가 18농가, $6{\sim}9%$ 미만이 5개 농가였고 나머지 3개 농가는 9%이상의 건물함량을 보여 대부분의 돈분액비는 낮은 건물함량을 함유하고 있는 것으로 나타났다. 액비 중 건물함량은 EC, $NH_4-N$, T-P, Ca, Mg, Na과 높은 상관성을 보였으며(p<0.01), 또한 전기전도도(EC)도 Mg을 제외한 모든 성분간의 유의성을 보였다. 따라서 건물함량과 전기전도도는 액비의 양분함량의 평가지표로 사용될 수 있음을 시사 하였다.