• Title/Summary/Keyword: 액체추출

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Culture Condition and Inoculum Volume of Liquid Spawn on the Bottled Cultivation of Agrocybe cylindracea (버들송이(Agrocybe cylindracea)의 액체종균 배양조건과 접종량)

  • Cheong, Jong-Chun;Hong, In-Pyo;Jang, Kab-Yeul;Park, Jeong-Sik
    • The Korean Journal of Mycology
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    • v.31 no.2
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    • pp.94-97
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    • 2003
  • In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.

Purification of Crude Protein Mixture from Panax ginseng and Hairy Root for Proteome Analysis (인삼 및 모상근의 프로테옴 분석을 위한 단백질 추출 방법)

  • 김승일;김수정;남명희;서종복;김수현;권경훈;김영환;최종순;유종신
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.347-351
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    • 2001
  • Panax ginseng C.A. Meyer is a well-known Korean traditional medicine. Until now, even though major research of ginseng has been focused on the pharmacological effect, clinical application and chemical analysis of extracted secondary metabolite for several years, the physiology and gene functions of ginseng were not well known. In this research, we have developed the protein extraction methods of ginseng root and hairy root for proteome analysis in order to elucidate the gene(s) function of ginseng. Using the liquid nitrogen (equation omitted) TCA method as protein extraction method, about 660 protein spots were detected on the 2-DE gel of hairy root. Additionally, comparative analysis result of 2-DEs of ginseng root (equation omitted) hairy root suggested that proteomes of same organism could be changeable according to the culture condition, growth stages and other stimulus.

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Application of Paint Spray Gun to Atomization of Barley Tea Concentrate (이류식(二流式) 노즐을 이용(理用)한 보리차 추출액(抽出液)의 분무(噴霧)에 관(關)한 연구(硏究))

  • Lee, Jeong-Cheol;Chun, Jae-Kun
    • Korean Journal of Food Science and Technology
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    • v.15 no.4
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    • pp.348-352
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    • 1983
  • The atomization of barley tea concentrate with paint spray gun of two fluid nozzle was investigated. The physical properties of barley tea concentrate were correlated to the operating parameters; soluble solid concentration, viscosity, specific gravity and surface tension. The air to liquid feed ratios at various barley tea concentration were correlated to the air pressures applied. The diameters of sprays at various air pressures (from 164 mmHg to 564 mmHg) and soluble solid concentrations (from 3.1% to 25.2%) were also investigated.

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용매추출법을 이용한 지하수중의 Polonium 측정

  • Jo Su-Yeong;Lee Gil-Yong;Yun Yun-Yeol;Kim Yong-Je
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.359-362
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    • 2005
  • 알파전용 액체섬광기를 이용하여 지하수내 Polonium을 간편하고 신속하게 측정하는 방법을 연구하였다. 추출섬광용액으로 HDEHP, TNOA, TOPO 또는 NDA 등을 사용하지만 본 실험에서는 POREX(77g TOPO, 180g Naphthalene, 4.0g PBBO/1L Toluene)를 사용하였다. 지하수의 액상을 $1M\;H_2SO_4-0.01M\;NaCl$로 맞추어주고 0.012M POREX를 추출액으로 사용하여, 분리 추출하므로써 실험의 추출효율 및 재현성을 조사하였다. 위 조건에서의 Polonium 분배계수는 $10^4$ 이상으로 알려져 있다. 표준물질인 Po-209(NIST SRM 4326)를 Po의 측정효율 및 추출효율을 조사하는데 이용하였다. 실제 지하수에 위 실험방법을 적용하여 지하수의 Polonium을 측정하였다.

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Thermal-Hydraulic Performance Analysis of KALIMER Conceptual Design Cores and Subassemblies (액체금속로 KALIMER 개념설계 노심 및 집합체 열유체 특성 분석)

  • 임현진;김영균;김영일;오세기
    • Journal of Energy Engineering
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    • v.13 no.2
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    • pp.101-111
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    • 2004
  • The main purpose of a liquid metal reactor core thermal-hydraulic design is to efficiently extract the core thermal power by distributing the appropriate sodium coolant flow according to the power distribution in the core. The thermal-hydraulic design procedure consists of the coolant flow distribution to the sub-assemblies, the coolant/fuel temperature calculations and detailed subchannel analysis. This paper describes the LMR core thermal-hydraulic design methodology and summarizes the major design and analysis results of KALIMER breeder and breakeven cores and subassemblies. KALIMER is a 150 MWe rated (392 MWth) heterogeneous core with U-TRU-Zr ternary alloy fuel and sodium coolant.

Studies on the Simultaneous Analysis of Organochlorine Pesticide Residues by Gas-Liquid Chromatography (I). Solvent Extraction and Cleanup of Pesticides (기체-액체 크로마토그래피에 의한 잔류 유기염소제 농약들의 동시 분석에 관한 연구 (제1보). 농약의 용매추출 및 불순물 제거)

  • Taek-Jae Kim;Song-Ja Park;Young-Sang Kim
    • Journal of the Korean Chemical Society
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    • v.29 no.5
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    • pp.496-502
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    • 1985
  • The solvent extraction and cleanup processes for the simultaneous gas-liquid cliromatographic determination of 16 kinds of organochlorine pesticide residues were investigated. The pesticides were extracted out from-various crops with the aqueous acetone solution acidified (pH < 1.5) by adding conc. $H_3PO_4$. Most of the pesticides were partitioned from the solution with petroleum ether. Evaporated the extracting solvent, the residues were dissolved in ethylether-petroleum ether (6 : 94) eluent and eluted through the Florisil column activated at 650$^{\circ}$C for 2.5hrs. The extraction efficiency was over 94% and impurities were effectively removed by the column chromatography.

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Antimicrobial Activity of Herb Extracts (허브추출물의 항균 작용)

  • Yoo, Mi-Young;Jung, Yoon-Ju;Yang, Ji-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.8
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    • pp.1130-1135
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    • 2005
  • The objective of this study was to evaluate the antimicrobial activity of four herb extracts from lavender, patchouli, rosemary and eucalyptus possibly used as food preservatives. Minimum inhibitory concentration of France rosemary extracts was 29.1 mg/mL against C. albicans ATCC 10231 and 14.5 mg/mL against B. subtilis ATCC 6613 and E. coli ATCC 25922. Both of France rosemary and American lavender herbal extracts were thermally stable between 40$^{\circ}C$ and 121$^{\circ}C$ and were stable at only neutral pH. Microbial growth was repressed by adding 2.9 mg of a commercial herbal extracts in 20 mL liquid medium with France rosemary extracts and American lavender extracts.

Enhancement of Anticarcinogenic Potentials of Submerged-Liquid Culture of Agaricus blazei Murill on Mouse Ascites Cancer by Rice Hull (왕겨에 의한 신령버섯균사체 액체배양액의 생쥐 항복수암성 증가)

  • Kim, Young-S.;Jang, Wook-J.;Rakib, A.;Kwon, Jung-M.;Ahn, Chae-R.;Kim, So-Y.;Cho, Yong-U.;Ha, Young-K.;Kim, Jeong-O.;Ha, Yeong-L.
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1402-1408
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    • 2010
  • The effects of rice hull (RH) powder on the anticarcinogenic activity of submerged-liquid cultures of Agaricus blazei Murill (AB) were assessed for mouse ascites cancers induced by mouse Sarcoma S-180 (S-180) cancer cells. Optimal growth of AB mycelia in the basal liquid culture medium, containing soybean meal, was achieved by culturing at $25^{\circ}C$ for 5 days, when evaluated by $\beta$-glucan content, Brix, and mycelial weight, relative to other culture conditions. Hot-water extract (HWE) of the submergedliquid culture of AB mycelia grown at $25^{\circ}C$ for 5 days exhibited a stronger anticarcinogenic activity, relative to HWE from other culture conditions. No such effects were obtained from AB mycelial cultures by alternative temperature-controlling cultures. Both cytotoxicity for S-180 cells and anticarcinogenic potentials for mouse ascites cancer of the HWE from AB mycelia grown in the basal medium containing 1% RH powder for 5 days at $25^{\circ}C$ were significantly (p<0.05) enhanced, relative to HWE from the AB mycelia culture of the basal medium without RH powder. These results indicate that HWE of submerged-liquid culture of AB mycelia, incubated in media containing 1% RH powder at $25^{\circ}C$ for 5 days, enhanced anticarcinogenic activity against S-180 cell-induced mouse ascites cancer, and suggest that RH powder is an excellent ingredient for the improvement of the anticarcinogenic potentials of the submerged-liquid culture of mushroom mycelia.