• Korean journal of food and cookery science
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• v.22 no.5 s.95
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• pp.720-727
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• 2006

The purpose of this study was to investigate the functional properties of medicinal plant extracts. Four kinds of medicinal plants, Dioscorea batatas(DB), Armeniacae Semen(AS) Crataegus pinnatifida Bunge(CP) and Ponciri Fractus(PF), were extracted with water and 70% ethanol and the extracts were tested for their electron donating ability(EDA), nitrite scavenging ability(NSA) and inhibitory effects on cancer cells(MDA cell and A549 cell)growth. EDA at 100-1,000 ppm of water extract ranged from 3% to 14%, 14% to 36%, 29% to 72% and 14% to 43%, and that of ethanol extract ranged from 9% to 62%, 27% to 59%, 33% to 89% and 14% to 44%, in DB, AS, CP and PF, respectively. NSA of extracts measured at various pH(1.2, 3.0, 4.2, 6.0) showed the highest ability in all extracts at pH 1.2 and decreased with increasing pH. The highest NSA of water extracts of 1,000 ppm at pH 1.2 was 6%, 31%, 55% and 44% and that of ethanol extract was15%, 32%, 69% and 52%, in DB, AS, CP and PF, respectively Inhibition ratio of water and ethanol extracts on MDA cell growth was 24% and 17%, 51% and 93%, 46% and 69%, and 48% and 47%, while that on A549 cell was 18% and 9%, 6% and 3%, 7% and 3%, and 43% and 11%, at 1,000 ppm, in DB, AS, CP and PF, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.8
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• pp.1293-1296
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• 2005

Nitrite plays an important role in the formation of carcinogenic nitrosamine. In order to effectively inhibit the formation of carcinogenic nitrosamine in food and biological systems, scavenging of residual nitrite is necessary. In screening test of nitrite-scavenging effect of seaweed, The methanol extract from Phaeophyta was more effective than those from Rhodophyta and Chlorophyta. Among the brown algae family, Laminariaceae, A. cribrosum (90.4$\%$) and E. cava (80.1$\%$), E. stolonifera (79.0$\%$) that belong to genus Ecklonia showed marked nitrite-scavenging effect at pH 1.2. Furthermore, these algae showed potent reducing power and radical scavenging activity. The nitrite-scavenging effect was correlated with level of reducing power.

• Journal of Mushroom
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• v.13 no.4
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• pp.330-333
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• 2015

This study was carried out to compare the anti-inflammation effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. We concentrated Ganoderma species and other medicinal mushrooms by extracting with ethanol. And We made it $100{\mu}g/ml$ concentration. As a result of nitrite scavenging activity, in the contrast to the positive control; Ascorbic acid was 25%, ASI 7080 of Ganoderma species was disappeared up to around 40%. And in the contrast to Ascorbic acid was 55%, ASI 7002 was 78.5% that was the highest anti-inflammation effect in the result of "No assay test". The Cordyceps militaris showed 75% and Hericium erinaceus showed 59.7% of anti-inflammation effect. As a result of the fungus yield control test of $TNF-{\alpha}$ through ELISA method to ASI 7002 of Ganoderma species that showed the highest anti-inflammation, it was reduced as same as LPS non-treatment. We extracted RNA from ASI 7002 Ganoderma species 10, 50, $100{\mu}g/ml$ concentration and LPS $10{\mu}g/ml$ of Raw 264.7 cell. And we tested the expression of iNOS, COX-2 and TNF-a that are kinds of inflammation gene after synthesizing RNA with cDNA. Finally we could find that iNOS, COX-2 and TNF-a were all controlled expression in the result of above experiment.

• Food Science and Preservation
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• v.13 no.3
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• pp.413-419
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• 2006

This study was investigated to analyze the antioxidant activity or extracts form Elaeagnus multiflora Thunb for development to the functional materials. The antioxidative activities of water ethanol and methanol extracts from the Elaeagnus multiflora Thunb were analyzed by electron donating ability (EDA), anti-oxidization activity, superoxide dismutase (SOD)-like activity, The superoxide anion radical-scavenging activity, and nitrite scavenging ability. The Etectron donating ability of methanol extract was higher in 1.0 mg/mL of extraction solution than those of ethers. The anti-oxidization activity of ethanol and methanol extracts by thiocyanate method using linolenic acid system was higher than those of the water extract. The SOD-like activity was increased with increase of the extract concentration in each extracts. The SOD-like activity was highest in 2.0 mg/mL of methanol extract. The superoxide anion radical-scavenging activity was increased with increase of the concentration in the ethanol extract and methanol extract. The nitrite scavenging ability of water extracts in 1.0 mg/mL of extraction soiution in pH 1.2 was higher than ethanol extrats and methanol extracts. The nitrite scavenging ability of all extracts was decreased according to increase of pH.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.12
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• pp.1503-1510
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• 2007

This study was carried out to investigate antioxidant substances (phenolic compounds and brown pigment), antioxidative effect and nitrite scavenging ability (NSA) of solvent extracts (hexane: HE, methanol; ME, water: WE) from fermented soybean foods, Chungkukjang and Doenjang. The antioxidant activities were determined by the measurements of peroxide value (POV) on the linoleic acid system, electron donating ability (EDA) and superoxide dismutase (SOD)-like activity. The contents of phenolic compounds and total brown pigments in Doenjang were $28.5{\pm}0.3mg/100g$ and optical density (OD) $1.98{\pm}0.03$, respectively, whereas their contents in Chungkukjang were significantly lower with $5.8{\pm}0.3mg/100g$ and OD $0.95{\pm}0.02$. The brown pigment contents of hydrophilic extracts increased more than that of lipophilic extracts through the fermentation and ripening processes. The ME of Doenjang exhibited higher inhibitory effects against the peroxidation of a linoleic acid system than that of Chungkukjang. The EDA and NSA of MEs were higher than those of WEs. Among the MEs, Doenjang showed the highest levels of EDA and NSA. On the other hand, the SOD-like activities of WE were higher than those of ME at the same concentrations and their activities of Doenjang were significantly higher than that of Chungkukjang. In conclusion, the antioxidative effects and NSA of Doenjang was significantly higher than those of Chungkukjang. It seemed that phenolic compounds and brown pigments formed during fermentation and ripening partly affect the antioxidative activities of fermented soybean foods.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.185-190
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• 2017

Antioxidative ability of 50% ethanol extract from leaves of Leucaena leucocephala (Lam.) de Wit was investigated. The extraction yield, total polyphenols and total flavonoids of the ethanol extract were $18.3{\pm}0.40%$, $121.3{\pm}11.04{\mu}g$ gallic acid equivalents/mg and $105.2{\pm}2.77{\mu}g$ quercetin equivalents/mg, respectively. The ethanol extract possessed the antioxidative activities such as free radical, cation radical, and nitrite scavenging activities as well as reducing power and lipid peroxidation inhibitory activity. These antioxidative activities of the ethanol extract increased in proportion to the extract concentration. $EC_{50}$ values for free radical scavenging, cation radical scavenging nitrite scavenging, and reducing power were $133.51{\mu}g/mL$, $667.22{\mu}g/mL$, 2.58 mg/mL, and $250.00{\mu}g/mL$, respectively. Polyphenols and flavonoids are suggested to be the key contributors for the antioxidative ability of the ethanol extract from Leucaena leucocephala leaves.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.167-172
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• 2019

In vitro antioxidant effect of 50% ethanol extract from Napier grass (Penninsetum purpureum) was investigated. The yield and polyphenol content of the Napier grass extract were $6.3{\pm}0.35%$ and $79.6{\pm}3.65{\mu}g$ gallic acid equivalents/mg-extract, respectively. Antioxidant ability of the Napier grass extract such as fee radical and cation radical scavenging activities, reducing power, nitrite scavenging activity, and lipid peroxidation inhibitory activity proportionally increased as concentration of the extract increased. $EC_{50}$ values of the Napier grass extract for free radical scavenging, cation radical scavenging, reducing power, and nitrite scavenging were 1,930.0, 350.0, 840.0, and $1,470.0{\mu}g/mL$, respectively. In the presence of $85.0{\mu}g/mL$ of the Napier grass extract, lipid peroxidation was potently inhibited by 74.6%.

• Food Science and Preservation
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• v.13 no.5
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• pp.548-554
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• 2006

This study investigated the effect of the addition of germinated brown rice extract powder (0, 0.67, 3.35 and 6.70%) to the fermentation of Korean cabbage kimchi. During the fermentation of Korean cabbage kimchi with GBREP, content of total free amino acid and essential amino acid was showed higher value by increasing the addition of GBREP. In particular, ${\gamma}$-aminobutyric acid (GABA) content of Korean cabbage kimchi, prepared with 6.70% GBREP was 2 times higher than control Korean cabbage kimchi. By increasing the content of GBREP, total phenol content was showed high value and the content was more increased during fermentation except control Korean cabbage kimchi. By increasing the content of GBREP, electron donating activity and superoxide radical scavening activity were showed higher value and then showed maximum scavening activity on palatable period. Nitrite-scavenging ability of Korean cabbage kimchi with GBREP was higher than control Korean cabbage kimchi and showed the highest scavenging ability at pH 1.2.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.489-493
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• 2003

This study was to search antioxidant constituents of ethyl acetate extract from Stachys siebodlii MIQ. The test of nitrite scavenging abilities were performed on the extracts of methanol, hexane, chloroform, ethyl acetate, butanol, and water, Ethyl acetate extract, The most promising one was fractionated on a silical gel column using elution solvent(chloroform:methanol:water=70:30:5 lower phase) at a flow rate 1.0ml/min. UV-VIS spectral data of each fraction showed adsorption maxima in the range of $284{\sim}330nm$ which is the characteristic range of $210{\sim}290nm$ and $300{\sim}550nm$ phenolic compounds. These results suggested that Stachys siebodlii MIQ. shows natural antioxidant activity. The nitrite scavenging abilities reached the maxium at pH 1.2 and the ethyl acetate fraction of root showed stronger scavenging ability.

• Korean journal of food and cookery science
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• v.16 no.4
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• pp.372-377
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• 2000

Six kinds of herbs(applemint, geranium, lemonbalm, rosemary, sage and thyme) were extracted with water and 95% methanol, and the electron donating ability, antibacterial activity and nitrite scavenging effect were examined. The electron donating ability of herbs ranged from 60.7 to 92.2% and the highest value was observed in geranium, followed by sage and lemonbalm. Antibacterial activity of methanol extracts was much stronger than that of water extracts and gram positive bacteria were more sensitive than gram negative bacteria. Rosemary and geranium exhibited excellent antimicrobial activities against a wide range of bacteria. Nitrite scavenging activity, which was measured at various pH conditions(1.2, 3.0, 3.6, 4.2), was the highest in all herbs at pH 1.2 and the lowest at pH 4.2, suggesting that it is pH dependent. Methanol fraction also provided higher nitrite scavenging effect than the water fraction.