• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.77-77
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• 2020

본 연구에서는 비트 새싹 추출물의 항산화 및 항염증 효능을 평가하기 위해 3가지 품종의 레드 비트(메를린, 우단, 디트로이트다크레드) 새싹을 60% 에탄올로 추출한 후 free radical 소거활성, 총 폴리페놀 함량을 측정하였다. 또한, LPS로 염증반응이 유도된 대식세포인 RAW264.7 세포에서 nitric oxide(NO) 생성억제 효과를 알아보았다. 메를린, 우단, 디트로이트다크레드 새싹 추출물의 총 폴리페놀 함량을 querceitn을 표준물질로 사용하여 측정한 결과 2.84 mg Que/g, 2.61 mg Que/g, 2.71 mg Que/g으로 나타났고 DPPH 라디칼 소거능은 농도 의존적으로 증가하였으며, 200 ㎍/ml일 때, 55%, 72%, 63%로 나타났다. 또한, DPPH 라디칼 소거능이 가장 높은 메를린 새싹 추출물은 LPS로 염증반응이 유도된 RAW264.7 세포에서 농도 의존적으로 iNOS와 COX-2를 억제하는 것을 확인하였다. 이러한 결과를 바탕으로 메를린 비트 새싹 추출물이 항산화 및 항염증제의 천연물 소재로 활용이 가능할 것으로 생각된다.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.79-79
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• 2003

생쥐 비장세포에 대한 아열대산 귀뚜라미(Gryllus bimaculatus)와 왕귀뚜라미 (Teleogryllus emma)로부터 3종 추출물(Al, A2, Bl)의 면역활성능을 조사하였다. 3종의 추출물 모두 normal spleen cell에 대하여 독성을 나타내지 않았다. Al과 A2는 세포의 proliferation을 증가시키는 경향을 나타내었으며, 1000ug/m1의 고농도에서는 약 10-20%의 증가를 보였다. Bl의 경우는 약간의 감소경향을 나타내었으며, T cell의 mitogen인 Con A에 대한 mitogenic response는 두드러진 활성을 나타내지 않았다. (중략)

• Journal of Applied Biological Chemistry
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• v.66
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• pp.250-257
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• 2023

Glutamate is an excitatory neurotransmitter distributed in the central nervous system of mammals. However, high concentrations of glutamate are known to cause neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and stroke by causing nerve cell death. In this study, the antioxidant activity and neuroprotective effect of subtropical natural products were analyzed. Among 11 subtropical plant extracts mainly tested, Sallacca wallichiana extract (SE) showed the greatest free radical scavenging activity. Then, we confirmed through WST-1 assay that SE protected HT22 cells against glutamate-induced cell death in a concentration-dependent manner. The protective effects of SE against glutamate-induced apoptosis in HT22 cells were also confirmed by flow cytometry analysis using Annexin V/PI double staining. We also confirmed using H₂DCF-DA single staining that SE inhibits glutamate-induced intracellular reactive oxygen species. And we were confirmed through that SE inhibited glutamate-induced phosphorylation of Mitogen-activated Protein kinases. Consequently, our results propose that SE may contribute to the development of therapeutics to prevent neurodegenerative diseases.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.12
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• pp.1937-1941
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• 2014

The effects of Tabebuia avellanedae (Taheebo) on exercise endurance capacity were investigated using mice in an adjustable-current water pool. Compared to the control group, a 1.8~2.0 fold increase in swimming time was observed in mice administered hot water extract (TAW) and 80% ethanol extract (TAE) of inner bark from T. avellanedae. Blood lactate level, an important fatigue relevance factor, was significantly lower in the TAW and TAE groups than in the control group. The total phenolic contents of TAW and TAE were $93.3{\pm}1.6$ and $115.7{\pm}1.5mgGAE/g$, respectively. The levels of flavonoids in the extracts were $77.3{\pm}1.3$ and $95.9{\pm}1.7mgCE/g$, respectively. Higher antioxidant activities of TAE were observed in each assay at the same concentration as compared to TAW. Correlation between antioxidant activities of TAE with total polyphenol contents was observed. These results suggest that extracts of Taheebo increase exercise endurance capacity by elevating antioxidative potentials.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.397-403
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• 2022

Smilax sieboldii is one of the Smilax species. A number of Smilax plants have long been used in traditional medicine in the tropics and subtropics worldwide. Repeated dose oral toxicity test is an essential experiment for toxicity evaluation before efficacy evaluation. The purpose of this study is to evaluate toxicity and the no-observed adverse effect level (NOAEL) using oral administration of Smilax sieboldii extract (SSE) in male and female ICR mice for 4 weeks. SSE was orally administered daily for 4 weeks at a dose of 500, 1000, and 2000 mg/kg/day (MPK). There were no significant differences in mortalities, clinical signs, body weight changes, food intake, hematological analysis, serum clinical chemistry test and relative organ weights in all animals administrated with SSE. The results obtained in this study suggest that SSE did not show any toxic effect in ICR mice and the NOAEL of SSE was regarded as over 2000 MPK.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.1
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• pp.49-58
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• 2024

Elaeocarpus sylvestris var. ellipticus is an evergreen tree of the family Elaeocarpaceae, which is a plant that grows naturally only in subtropical regions due to its vulnerability to cold. E. sylvestris has been reported to have flavonoids, coumarins, and polyphenols, and it is reported that these components have antibacterial and antioxidant effects. In this study, a roasting technique was introduced to increase the physiological activity of E. sylvestris, and antioxidant, total phenol, and total flavonoid content were measured after confirming changes in the ingredients of roasted E. sylvestris extracts and fractions. We analyzed the extracts before and after roasting and found an increase in the content of four components (brevifolin, ellagic acid, quercetin, and kaempferol), with the roasted extracts showing better antioxidant activity. The optimal roasting condition was confirmed to have the best antioxidant effect when roasting at 200 ℃ for 30 min, and the total phenol and total flavonoid content were also confirmed to be the best. E. sylvestris extract produced under optimal roasting conditions has been confirmed to exhibit improved antioxidant effects, and it is believed that it can be used as a cosmetic and food material in the future.

• Food Science and Preservation
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• v.22 no.4
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• pp.577-584
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• 2015

The objective of this study was to investigate the antioxidant activity of imported tropical and subtropical fruits including dragon fruits, green kiwi, papaya, pineapple, pomegranate, and yellow mango. A seventy percent of ethanol extracts were prepared. Total phenolic content, DPPH (1,1-diphenyl-2-picryl hydrazyl)-, ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), superoxide anion radical scavenging activity, FRAP (ferric reducing antioxidant power), tyrosinase inhibitory activity, and reducing power were investigated for the comparisons of antioxidant activities. The phenolic content expressed as gallic acid equivalents (GAE) was found to be highest in pomegranate (12.22 mg GAE/g), followed by pineapple (3.77 mg GAE/g). Pomegranate and pineapple exhibited higher antioxidant activity than those of other fruits except for FRAP. DPPH and ABTS radical scavenging activity from pomegranate were 93.00%, and 98.98%, respectively, at a concentration of 5 mg/mL, which were equal to those of ascorbic acid used for a positive control.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.68-77
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• 2005

A characteristic of subtropical zones region MK-T2 compares with an gaeryangppong, and the 9-10 schedule the times when a leaf blooms to are fast, and ratio that a branch edge by the colds becomes lean showed 5.7%, and a growth of the new branch which went out delivers 67.2 cm, mulberry loaves of the new branch which went out, and 18.6, a form of a leaf is the 1.10 that length of a leaf grew more a bit than width of a leaf up. Thickness of a leaf is $228.2{\mu}m$, and an area is more similar than gaeryangppong as $225.6cm^2$. in plant taxonomy, the hair whom the style exists short with 0.7 mm, and go to the pistil head inside so as to be rare is distributed, and belong to Dolichostylae Pubescentes. The new branch cutting which executed without remedy processes was independent of a thickness of a case branch, and the form and 100% root was said, and an gaeryangppong compared with the fact that 10% root went out of 15 mm ideal, and was excellent very, and looked, a root went out a root the soil and water, all showed a characteristic to go out at central of a branch bases at 45% ratio. Length was 24.6 mm, and were water rate 78.8%, and mulberry of MK-T2 was carrying together sweetness and acidity to pH 4.7 while, besides, arrival was 19.21 Brix%. A larva period and pupa ratio, cocoon thickness ratio are almost similar to gaeryangppong, or weight of one cocoon, cocoon thickness, 20,002 cocoon quantity shows some results to drop, and be soft of a leaf, and feed value certifications are comparatively top-ranking. As a result of having analyzed amino acid of the 3rd day of 5th silkworm larva which bred to MK-T2, a collation absorbing an gaeryangppong went, and looked, but compared with a collation in case of tests to eat MK-T2, and looked, and the lie collations were not detected a difference at Leu, but MK-T2 tests were detected mutual almost similar amino acid creation. medical efficacy of the 3rd day of 5th silkworm larva ethanol extract which bred to MK-T2 and black results, histologic a case did not appear at HE dyeing about the kidney organization which extracted form the rats which ate a silkworm ethanol extract and dyeing all chemical organization immunity, and one step protein revelation became lower with almost unidentified levels.

• Journal of Life Science
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• v.28 no.1
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• pp.50-57
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• 2018

Millettia erythrocalyx, a species of plant in the Fabaceae family, is widely distributed in the tropical and subtropical regions of the world, such as the Indies, China, and Thailand. The antiviral activity of flavonoids from M. erythrocalyx has been reported; however, the antioxidative and anticancer activities of M. erythrocalyx remain unclear. In this study, we evaluated the antioxidative and anticancer effects of ethanol extract of M. erythrocalyx (EEME) and the molecular mechanism of its anticancer activity in human hepatocellular carcinoma HepG2 cells. EEME exhibited significant antioxidative effects, with a concentration at 50% inhibition ($IC_{50}$) value of $2.74{\mu}g/ml$, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay; moreover, it inhibited cell proliferation in a dose-dependent manner in HepG2 cells. Cell cycle analyses showed that EEME induced HepG2 cell accumulation in the subG1 phase in a dose-dependent manner. EEME also induced apoptosis of HepG2 cells, with increases in apoptotic cells and apoptotic bodies, as detected by Annexin V and 4,6-diamidino-2-phenylindole (DAPI) staining, respectively. Treatment with EEME resulted in increased expression of First apoptosis signal (Fas), a death receptor, and Bcl-2-associated X protein (Bax), a proapoptotic protein, and the activation of caspase-3, 8, and 9, resulting in the cleavage of poly (Adenosine diphosphate-ribose) polymerase (PARP). Collectively, these results suggest that EEME may exert an anticancer effect in HepG2 cells by inducing apoptosis via both the intrinsic and extrinsic pathways.

• Journal of Plant Biotechnology
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• v.47 no.3
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• pp.248-253
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• 2020

Moringa (Moringa oleifera), which is native to tropical and subtropical climates, now grows in Korea due to its warming climate. Since it is also imported and used for food and other products in various countries, it is necessary to analyze the properties of moringa being imported into Korea. In this study, the antioxidant activity and total phenol and flavonoid content were high in the leaves and twigs and showed a positive correlation. The mineral content was also high in the leaves and twigs, and potassium ions were the most abundant mineral. In addition, seven phenolic acids (caffeic acid, chlorogenic acid, cinnamic acid, coumaric acid, ferulic acid, gallic acid, and syringic acid) and four flavonoids (kaempferol, myricetin, quercetin, and rutin) were detected in the leaves, twigs, and stems, and the ferulic acid and quercetin content was particularly high. The results of this study can be used as comparative data for domestic moringa growers in the future.