Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Assessment of the 4-week repeated dose oral toxicity test of Smilax sieboldii extract in ICR mice

ICR 마우스에서 청가시덩굴 추출물의 4주간 반복 투여 독성시험

  • Jung A, Lee (Bio-center, Gyeonggido Business and Science Accelerator) ;
  • Min-Hee, Hwang (Bio-center, Gyeonggido Business and Science Accelerator) ;
  • Young-Rak, Cho (Bio-center, Gyeonggido Business and Science Accelerator) ;
  • Eun-Kyung, Ahn (Bio-center, Gyeonggido Business and Science Accelerator)
  • Received : 2022.11.04
  • Accepted : 2022.12.01
  • Published : 2022.12.31
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Abstract

Smilax sieboldii is one of the Smilax species. A number of Smilax plants have long been used in traditional medicine in the tropics and subtropics worldwide. Repeated dose oral toxicity test is an essential experiment for toxicity evaluation before efficacy evaluation. The purpose of this study is to evaluate toxicity and the no-observed adverse effect level (NOAEL) using oral administration of Smilax sieboldii extract (SSE) in male and female ICR mice for 4 weeks. SSE was orally administered daily for 4 weeks at a dose of 500, 1000, and 2000 mg/kg/day (MPK). There were no significant differences in mortalities, clinical signs, body weight changes, food intake, hematological analysis, serum clinical chemistry test and relative organ weights in all animals administrated with SSE. The results obtained in this study suggest that SSE did not show any toxic effect in ICR mice and the NOAEL of SSE was regarded as over 2000 MPK.

청가시덩굴은 청미래덩굴속에 속하며 청미래덩굴속 식물들은 전세계적으로 열대 및 아열대 지역에 서식하며 전통 의학에서 오랫동안 사용되어 왔다. 본 연구의 목적은 수컷과 암컷 ICR 마우스를 이용하여 청가시덩굴의 안전성을 확인하기 위해 청가시덩굴 추출물을 4주 동안 경구 투여하여 최대무독성용량을 평가하였다. 청가시덩굴 추출물을 500, 1000 및 2000 MPK 용량으로 4주 동안 매일 경구 투여한 결과 투여한 모든군에서 사망률, 임상 증상, 체중 변화, 섭취량, 혈액학적 분석, 혈청 생화학 검사 및 상대 장기 중량에 유의한 차이가 없었다. 본 연구에서 청가시덩굴 추출물은 ICR 마우스에서 어떠한 독성 효과도 나타내지 않았으며 청가시덩굴 추출물의 최대무독성용량은 2000 MPK 이상으로 간주되었음을 시사한다.

Keywords

Acknowledgement

본 연구과제는 산림청(한국임업진흥원) 산림과학기술 연구개발사업(Project No. 2021371B10-2223-BD02)의 지원에 의하여 이루어진 것입니다.

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