Browse > Article
http://dx.doi.org/10.3839/jabc.2022.051

Assessment of the 4-week repeated dose oral toxicity test of Smilax sieboldii extract in ICR mice  

Jung A Lee (Bio-center, Gyeonggido Business and Science Accelerator)
Min-Hee Hwang (Bio-center, Gyeonggido Business and Science Accelerator)
Young-Rak Cho (Bio-center, Gyeonggido Business and Science Accelerator)
Eun-Kyung Ahn (Bio-center, Gyeonggido Business and Science Accelerator)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 397-403 More about this Journal
Abstract
Smilax sieboldii is one of the Smilax species. A number of Smilax plants have long been used in traditional medicine in the tropics and subtropics worldwide. Repeated dose oral toxicity test is an essential experiment for toxicity evaluation before efficacy evaluation. The purpose of this study is to evaluate toxicity and the no-observed adverse effect level (NOAEL) using oral administration of Smilax sieboldii extract (SSE) in male and female ICR mice for 4 weeks. SSE was orally administered daily for 4 weeks at a dose of 500, 1000, and 2000 mg/kg/day (MPK). There were no significant differences in mortalities, clinical signs, body weight changes, food intake, hematological analysis, serum clinical chemistry test and relative organ weights in all animals administrated with SSE. The results obtained in this study suggest that SSE did not show any toxic effect in ICR mice and the NOAEL of SSE was regarded as over 2000 MPK.
Keywords
4-week repeated dose oral toxicity; ICR mouse; No-observed adverse effect level; Smilax sieboldii;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Bent S (2008) Herbal medicine in the United States: review of efficacy, safety, and regulation. J Gen Intern Med 23(6): 854-859. doi: 10.1007/s11606-008-0632-y   DOI
2 Lee CB (2003) Coloured Flora of Korea (2003) Korean Plant Encyclopedia, Hyangmunsa, Seoul
3 Kim JS, Kim TY (2011) korean tree. dolbegae, Suwon
4 Bluher M (2013) Adipose tissue dysfunction contributes to obesity related metabolic diseases. Best Pract Res Clin Endocrinol Metab 27: 163-177. doi: 10.1016/j.beem.2013.02.005   DOI
5 Okerson T, Patel J, DiMario S, Burton T, Seare J, Harrison DJ (2017) Effect of 2013 ACC/AHA Blood Cholesterol Guidelines on Statin Treatment Patterns and Low-Density lipoprotein Cholesterol in Atherosclerotic Cardiovascular Disease Patients. J Am Heart Assoc 6: e004909. doi: 10.1161/JAHA.116.004909   DOI
6 Cho YR, Lee JA, Kim YY, Kang JS, Lee JH, Ahn EK (2018) Anti-obesity effects of Clausena excavata in high-fat diet-induced obese mice. Biomed Pharmacother 99: 253-260. doi: 10.1016/j.biopha.2018.01.069   DOI
7 Park JH, Ahn EK, Hwang MH, Park YJ, Cho YR, Ko HJ, Jeong W, Yang SH, Seo DW, Oh JS (2021) Improvement of Obesity and Dyslipidemic Activity of Amomum tsao-ko in C57BL/6 Mice Fed a High-Carbohydrate Diet. Molecules 26(6): 1638. doi: 10.3390/molecules26061638   DOI
8 Yoo JG, Jung JY (2012) Studies on the repeated toxicity test of food red No.2 for 4 weeks oral administration in SD Rat. J Fd Hyg Safety 27: 42-49. doi: 10.13103/JFHS.2012.27.1.042   DOI
9 Ji KT, Kim KM, Oh JJ, Kim JW, Lee WJ, Cho H, Lee HK, Lee JY, Chae S (2020) Assessment of the 4-week repeated-dose oral toxicity and genotoxicity of GHX02. J Appl Toxicol 40(20): 270-284. doi: 10.1002/jat.3902   DOI
10 Aliyu A, Shaari MR, Ahmad Sayuti NS, Reduan MFH, Sithambaram S, Noordin MM, Shaari K, Hamzah H (2020) Subacute Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract Induced Liver and Kidney Toxicities in ICR Mice. Molecules 25(11): 2631. doi: 10.3390/molecules25112631   DOI
11 Park JH, Cho YR, Kim YM, Kang JS, Oh JS, Ahn EK (2019) 3-week repeated dose oral toxicity study of Clausena excavate extract in ICR mice. J Appl Biol Chem 62(2): 123-127. doi: 10.3839/jabc.2019.017   DOI
12 Lee JE, Kim HJ, Lee CH, Lee KC, Choi EK, Chai HY, Yun YW, Kim DJ, Nam SY, Lee BJ, Ahn BW, Chang HG, Kim YB (2003) Four-week repeated-dose toxicity study on Pinellia extract. Korean J Lab Anim Sci 19: 127-141
13 Roh HS, Seok JH, Jeong JY, Lee JK, Kim TS, Choi HK, Ha HY (2014) Repeated dose oral toxicity study of Alismatis rhizoma in SD Rats. J Korean Med Ophthalmol Otolaryngol Dermatol 27:79-90. doi: 10.6114/jkood.2014.27.1.079   DOI
14 Kim YB, Hong DH, Cho ES, Lm WJ, Kim IH, Son HY (2014) Effect of repeated Paecilomyces japonica treatment on rats. Korean J Vet Res 54:31-38. doi: 10.14405/kjvr.2014.54.1.31   DOI
15 Lee JS, Lee JS, Park YC, Choi SM, Lee SH (2014) A repeated-dose 28-day oral toxicity test of Aconitum jaluense extract in sprague-dawley rats. Yakhak Hoeji 58: 62-70