• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1535-1544
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• 2020

The primary objective of this study was to isolate and identify amine oxidase-producing probiotic Bacillus strains from traditional fermented soybean paste. Biogenic amines (BA)-forming bacteria isolated from the samples were identified as Bacillus sp. TS09, Bacillus licheniformis TS17, Bacillus subtilis TS19, Bacillus cereus TS23, Bacillus sp. TS30, Bacillus megaterium TS31, B. subtilis TS44, Bacillus coagulans TS46 and Bacillus amyloliquefaciens TS59. Meanwhile, B. subtilis TS04 and TS50 isolated from the same samples exhibited good probiotic properties, including the tolerance to artificial gastric juice and bile salts, the adhesion to intestinal epithelial cells, and the production of bacteriocin(s) active against BA-forming bacteria (Bacillus sp. TS30 and B. subtilis TS44). In addition, the amine oxidase produced by B. subtilis TS04 and TS50 significantly decreased the formation of BA, especially cadaverine, putrescine, and tyramine, therefore, these strains could be considered good potential probiotic candidates to prevent or reduce BA accumulation in food products.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.626-632
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• 2005

The polyamines are essential components of all eukaryotic cells and absolutely necessary for cell growth. In the present study, the cytoprotective role of polyamine was characterized. When $Ac_2F$ rat liver cells were treated with 1M 2,2'-azobis (2-amidinopropane) dehydrochloride (AAPH), a water soluble free radical initiator, viability of the cells was noticeably decreased due to the increase of reactive oxygen species (ROS). The cytotoxic effect of AAPH as well as ROS generation were significantly inhibited by the treatment of polyamines. Among polyamines, especially spermine at $20{\mu}M$ concentration exerted over $45\%$ inhibition of AAPH-induced ROS generation. Western blotting was performed to determine whether superoxide dismutase(SOD) or catalase (CAT) expression was involved in oxidative stress. The AAPH treatment blocked both SOD and CAT protein expressions. Spermine could recover those protein expressions to the untreated control levels. According to the result of cycline E measurement, AAPH might block the entry of the cells into S phase of the cell cycle. The reduced expression of cyclin E protein could be fully recovered by the addition of spermine. The antioxidative effects of spermine was also further proved by the apopotitic morphological analysis using ethidium bromide and acridine orange.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2001.11a
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• pp.295-296
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• 2001

동물 축사, 폐수 및 하수처리장, 피혁공장, 생선처리시설 등에서 주로 발생하는 암모니아(NH$_3$) 및 트리메틸아민(($CH_3$)$_3$N)과 같은 악취 물질 제거에 대한 많은 연구가 진행되고 있다. 악취 물질에 대한 처리 기술에는 직접 산화법, 고온 연소법, 효소분해법, 흡착법, 촉매 산화법, 플라즈마 제거법 등과 같은 악취 물질을 분해 제거하는 방법과 단순히 악취를 은폐시키는 마스킹법이 있다. (중략)

• Korean Journal of Food Science and Technology
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• v.44 no.2
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• pp.141-147
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• 2012

Biogenic amines have been used as chemical indicators to estimate bacterial spoilage of foods, particularly fish and fish products, cheese, and fermented foods. So far many chromatography methods have been developed to detect biogenic amines in foods. Although these instrumental analyses exhibit good sensitivity, they cannot be used as rapid detection methods due to the chemical treatment of the samples and the time-consuming process involved. For the rapid and simple detection of biogenic amines, enzyme linked immunosorbent assay kits are commercially available. In addition, analytical systems with enzyme-based amperometric biosensor detection have been increasingly developed. The biosensors used to detect the biogenic amines are based on the action of either amine oxidases or amine dehydrogenases that catalyzes the oxidative deamination of biogenic amines to the corresponding aldehydes and ammonia. This review mainly focused on the principle, development, and applications of the detection methods for rapid detection of biogenic amines in foods.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.6
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• pp.437-444
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• 2003

Objective of this experiment was to investigate the role of strontium in intracellular processes in mung bean (Vigna radiata L.). Diamine oxidase (DAO) induction by $Sr^{2+}$ appeared to a decrease in putrescine levels correspondently. DAO activities in the hypocotyls were in a range of 0.5 to $1.8unit{\cdot}mg^{-1}\;protein{\cdot}min^{-1}$. The decrease in Put levels in the cotyledons might be partly resulted from Put degradation by DAO. It was observed that the accumulation of spermidine and spermine by $Sr^{2+}$ was in the range of 1 mM to 10 mM. Spermidine levels were 2 to 3 fold higher than in the absence of strontium. The increase in polyamine levels was observed not only on a basis of g fresh weight but also a RNA basis. These results demonstrated that the inhibitory action of $Sr^{2+}$ may be closely related with polyamine metabolism as well as diamine oxidation and polyamine accumulation.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.17-24
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• 2019

Bacteria in the polar region are under strong light and ultraviolet radiation. In this study, we investigated the effects of light on the growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. The growth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonas sp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentration-dependent manner along with ascorbic acid. N-acetylcysteine had no effect on the growth of the strain in the light. Thiamine and ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The results of this study indicate that thiamine provided by the lichen symbiosis system plays an important role in light-induced oxidative stress in this Arctic bacterium as an antioxidant. Our study provide insight into the biochemistry and physiology of Arctic bacteria under strong light and ultraviolet radiation.

• Proceedings of the Korean Fiber Society Conference
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• 1998.04a
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• pp.338-341
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• 1998

악취의 주원인은 염기성 취기의 대표인 암모니아, 산성 취기의 대표인 황화수소, 그리고 트리메틸아민이며, 이들은 법정 악취물질로써 지정되어 있다$^1$. 이와 같은 악취를 없애는 소취기구는 활성탄 및 제오라이트 등과 같은 물질에 대한 물리적 흡착에 의한 것, 산화제 및 환원제에 의한 화학반응에 의한 것, 미생물 및 효소에 의한 생물학적 반응에 의한 것으로 대별되지만, 물리 흡착은 재방출의 문제가 있고 화학 및 생물학적 반응에는 소취성분 자체의 유해성 및 반응후 물질의 유해성이 문제가 되는 경우가 있다.(중략)

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.7-13
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• 2004

Mung beans (Vigna radiata L.) were used to investigate the roles of strontium in hypocolyl elongation under IAA regime during the germination. After imbibition in a medium with or without IAA, $Sr^{2+}$ stimulated IAA oxidation. Three to five fold increasing in IAA oxidase activity seems to be direct evidence of growth inhibition through $Sr^{2+}$. Furthermore, the accumulation of spermidinc and spermine by $Sr^{2+}$ in the range of 1 to 10 mM was observed. Spermidine levels were 2 to 3 fold higher than in control seedling grown without strontium. The increase in polyamine levels was observed on a g fresh weight basis. In conclusion, it was demonstrated that the inhibitory action of $Sr^{2+}$ is closely related with the IAA oxidation and polyamine biosynthesis.

• Journal of the Korean Electrochemical Society
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• v.17 no.1
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• pp.30-36
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• 2014

Redox complexes to transport electrons from enzyme to electrodes are very important part in glucose sensor. Pentacyanoferrate-bound aniline ($Fe(CN)_5$-aminopyridine), was prepared as a potential redox mediator in a glucose dehydrogenase (GDH)-glucose sensor. The synthesized pyridyl-$NH_2$ to pentacyanoferrate was characterized by the electrochemical and spectroscopic methods. A amperometric enzyme-linked electrode was developed based on GDH, which catalyses the oxidation of glucose. Glucose was detected using GDH that was co-immobilized with an $Fe(CN)_5$-aminopyridine and gold nano-particles (AuNPs) on ITO electrodes. The $Fe(CN)_5$-aminopyridine and AuNPs immobilized onto ITO electrodes provided about a two times higher electrochemical response compared to that of a bare ITO electrode. As glucose was catalyzed by wired GDH, the electrical signal was monitored at 0.4 V versus Ag/AgCl by cyclic voltammetry. The anode currents was linearly increased in proportion to the glucose concentration over the 0~10 mM range.

• Journal of the Korean Electrochemical Society
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• v.10 no.2
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• pp.150-154
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• 2007

Redox complexes to transport electrodes from biomaterial to electrodes are very important part in commercial biosensor industry. A novel osmium redox complex was synthesized by the coordinating pyridine group with osmium metal. A novel osmium complex is described as $[Os(dme-bpy)_2(ap-im)Cl]^{+/2+}$. We have been studied the electrochemical characteristics of this osmium complex with electrochemical techniques such as cyclic voltammetry and chronoamperommetry. In order to immobilize osmium redox complexes on the electrode, we deposited gold nano-particles on screen printed carbon electrode(SPE). The electrical signal converts the osmium redox films into an electrocatalyst for glucose oxidation. The catalytic currents were monitored that the catalytic currents were linearly increased from 1 mM to 5 mM concentrations of glucose.