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http://dx.doi.org/10.5352/JLS.2005.15.4.626

Cytoprotective Effects of Polyamines Against Oxidative Stress  

Ahn Seoni (Department of Biology, Pusan National University)
Lee Ji Young (Institute of Genetic Engineering Pusan National University)
Chung Hae Young (Institute of Genetic Engineering, Pusan National University, Department of Pharmacy, Pusan National University)
Yoo Mi-Ae (Institute of Genetic Engineering, Pusan National University, Department of Molecular Biology, Pusan National University)
Kim Jong-Min (Department of Anatomy and Cell Bilogy, College of Medicine, Dong-A University)
Kim Byeong Gee (Department of Biology, Pusan National University, Institute of Genetic Engineering, Pusan National University)
Publication Information
Journal of Life Science / v.15, no.4, 2005 , pp. 626-632 More about this Journal
Abstract
The polyamines are essential components of all eukaryotic cells and absolutely necessary for cell growth. In the present study, the cytoprotective role of polyamine was characterized. When $Ac_2F$ rat liver cells were treated with 1M 2,2'-azobis (2-amidinopropane) dehydrochloride (AAPH), a water soluble free radical initiator, viability of the cells was noticeably decreased due to the increase of reactive oxygen species (ROS). The cytotoxic effect of AAPH as well as ROS generation were significantly inhibited by the treatment of polyamines. Among polyamines, especially spermine at $20{\mu}M$ concentration exerted over $45\%$ inhibition of AAPH-induced ROS generation. Western blotting was performed to determine whether superoxide dismutase(SOD) or catalase (CAT) expression was involved in oxidative stress. The AAPH treatment blocked both SOD and CAT protein expressions. Spermine could recover those protein expressions to the untreated control levels. According to the result of cycline E measurement, AAPH might block the entry of the cells into S phase of the cell cycle. The reduced expression of cyclin E protein could be fully recovered by the addition of spermine. The antioxidative effects of spermine was also further proved by the apopotitic morphological analysis using ethidium bromide and acridine orange.
Keywords
antioxidant; apoptosis; polyamine; ROS; spermine;
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