• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.293-300
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• 2011

This study is conducted to develop an efficient transformation system via particle bombardment with PLBs (Protocorm-like bodies) in Cymbidium. For this, pCAMBIA3301 vector which carries a herbicide-resistant bar gene and gus gene as a reporter gene was used for transformation with Cymbidium cultivars 'Youngflower ${\times}$ masako' line. To select transformants, proper concentration of herbicide, PPT (phosphinotricin), should be determined. As a result, 5 mg/l of PPT was selected as a proper concentration. Further, proper conditions for particle bombardment were determined to obtain a high frequency of transformation. Results showed that 1.0 ${\mu}g$ of DNA concentration, 1,100 and 1,350 psi for helium gas pressure, 1.0 ${\mu}m$ of gold particle and 6 cm of target distance showed the best result for the particle bombardment experiment. Also, pre-treatment with combination 0.2 M sorbitol and 0.2 M mannitol for 4 hrs prior to genetic transformation increased the transformation efficiency up to 2.5 times. Using transformation system developed in this study, 3.2 ~ 4.0 transgenic cymbidium plants can be produced from 100 bombarded PLBs on average. Putative transgenic plants produced in this system confirmed the presence of the bar gene by PCR analysis. Also, leaves from randomely selected five transgenic lines were applied for Basta solution (0.5% v/v) to check the resistance to the PPT herbicide. As a result, three of them showed resistance and one of them showed the strongest resistance with the maintenance of green color as non-transformed plants showed. Using this established transformation system, more genes of interests can be introduced into Cymbidium plants by genetic transformation in the future.

• Research in Plant Disease
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• v.20 no.3
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• pp.227-233
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• 2014

The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 - 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 ($10^7$colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.715-718
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• 2010

$Cymbidium$ 'Purple Princess' ('Show Girl' x 'Eiko') is a new cultivar having dark purple sepal and petal (RHS, RP59A) with purple lip (RHS, RP59A). A cross was made between pink colored $C.$'Show Girl' as maternal line and dark purple colored $C.$ 'Eiko' as paternal line in 1994. It was preliminarily selected as Line No. 94019267 in 1999 considering the characters of flower shape and color, leaf growing shape and growth habit in normal culture condition. Multiplication, cultivation, and characteristic trials were conducted from 2000 to 2003. The line was named as Wongyo F1-7 and phenotype was characterized in 2004 as a new 'Purple Princess'. 'Purple Princess' has about 13.2 flowers per flower stalk and flower size of 5.2 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming starts from early December under optimal culture condition. Leaf attitude and twisting is erect and very weak respectively. Erect leaf will be suitable for growing and handling in the nursery as pot orchid flowers.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2004.03a
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• pp.228-232
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• 2004

• Horticultural Science & Technology
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• v.16 no.1
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• pp.40-41
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• 1998

This experiment was conducted to investigate the effects of growth retardant uniconazole on the growth and flowering of Cymbidium Pine Clash 'Moon Venus' and Cym. Green Sour 'A One'. Reduction in leaf length of both cultivars was proportional to the amount (0-5g) of uniconazole and growth reduction of 'A One' was more remarkable than 'Moon Venus'. The inflorescence length was reduced 18.4% in 'Moon Venus' and 15.3% in 'A One' by 5g treatment of uniconazole. There was no significant difference in flowering time, number of inflorescences and florets and size of lower sepal regardless of uniconazole treatment. It was concluded that uniconazole treatment is effective in reducing the leaf growth of Cymbidium. However, as high concentration (5g) of uniconazole reduced the growth of inflorescence causing low quality, 1g was recommended as useful concentration.

• Horticultural Science & Technology
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• v.17 no.3
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• pp.344-345
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• 1999

This experiment was conducted to investigate the effects of a growth retardant uniconazole treatment time on the growth and flowering of Cymbidium Pine Clash 'Moon Venus' and Cym. Green Sour 'A One' in order to develop a substitutional technique on the highland cultivation. Leaf growth of both cultivars was more remarkably reduced by earlier uniconazole treatment time and growth reduction of 'A One' was more remarkable than 'Moon Venus'. Flowering time of 'Moon Venus' treated with uniconazole at May 7 and 27 was accelerated by 5 and 6 days respectively. Moreover, the number of inflorescences and florets showed a tendency to increase. However there was no difference in flowering time of 'A One' among treatments but there was a tendency to decrease in the number of inflorescences and florets by uniconazole treatment. In both cultivars, there was no difference among treatments in the length and width of lower sepal which represent the floret size.

• Research in Plant Disease
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• v.13 no.2
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• pp.126-129
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• 2007

Sooty leaf blight disease of Cymbium spp. was observed on orchid fields located at Gyeonggi-do in 2005-2006. Symptoms of the disease appeared on leaves and leaf spots were circular to nearly-circular, these circular blemished were yellow, with greater amounts of brown to black flecks forming as the spots enlarge. Severely infected leaves were dry and defoliated. These symptoms were realized wrongly as symptoms by virus. But Pseudocercospora cymbidiicola were isolated from the diseased plants. Conidiophores were produced on the lesion surface of the leaf with the blemished areas andconidia formed dark brown, cylindrical and straight to slightly curved, 5-9 septate, $23.7-85.0\;{\times}\;2.0-3.4\;{\mu}m$. Mycelial growth was mostly slow on potato dextrose agar and the optimum temperature for growth was $25^{\circ}C$. We were identified as Pseudocercoepora cymbidiicola based on the morphological characteristics.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.163-171
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• 2016

We investigated the intensity and pattern of the scent produced by diploid and tetraploid Cymbidium flowers, using an electronic nose with 6 metal oxide sensors (MOS). The MOS responses were evaluated by principal component analysis, discriminant function analysis, and sensor data. These analyses revealed that tetraploid flowers had a stronger scent than diploid flowers in Cymbidium Golden Elf 'Sundust'. Furthermore, among the different flower parts-column, lip, and petals-the column produced the strongest scent. There was no significant difference between the flowering periods of diploid and tetraploid potted Cymbidium Golden Elf 'Sundust' and Cymbidium Elma 'Orient Toyo' grown in a greenhouse. Moreover, there were no significant differences between the number of flowers per flower stem and the length of flower stems on the diploid and tetraploid plants of these two Cymbidium cultivars. This study provides potentially useful information for the breeding of polyploidy Cymbidium in the floriculture industry.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.201-206
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• 2010

The genetic relationship among 48 Cymbidium cultivars was analyzed using randomly amplified polymorphic DNA (RAPD) with eighty 10 mers random primers (Operon Technologies) and twelve 20 mers random primers. Forty eight Cymbidium cultivars included 34 oriental Cymbidium, 7 hybrids, and 7 western Cymbidium. 407 (9.9 per primer) and 56 polymorphic bands (9.5 per primer) were generated by polymerase chain reaction with selected thirty 10 mers primers, and nine 20 mers primers, respectively. The polymorphic fragments ranged from 0.4 to 1.5 kb in size. The dendrogram was constructed by using the UPGMA clustering algorithm based on genetic similarity. Forty eight Cymbidium cultivars were classified into four major groups at similarity coefficient value of 0.638.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.291-294
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• 2008

Cymbidium 'Honey Girl' was developed in 2005 by the National Horticultural Research Institute, Rural Development Administration. This cultivar came from cross between Cym. 'Yakoto' and Cym. 'Marian Simplicity' in 1995. Fifty seven seedlings were obtained after planting and acclimatization in green house. In 1999, a line was selected by its performance such as flower color, leaf shape, flower stalk, and vigorous growth which was named 'Wongyo F1-13'. The line code '9521928'($V_2$) had uniformity and excellent characteristics. The selected line was named as 'Honey Girl' after second characteristics test. This cultivar had white color ('WN 155B') and medium sized leaves and flowers (average leaf length is about 57 cm and width of flower is about 7.7cm) with many flowers and erect flower stalks. Blooming begins from the end of November to December under the normal culture conditions.