• Title/Summary/Keyword: 실험동

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Insect and Invertebrate Fauna in Pine Mushroom (Tricholoma magnivelare) Habitat (송이버섯 자연 군락지의 곤충상)

  • Chung, Duck-Young;Kang, Myung-Gi;Park, Sun-Nam;Seo, Mi-Ja;Lee, Jong-Shin;Youn, Young-Nam
    • Korean journal of applied entomology
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    • v.45 no.2 s.143
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    • pp.153-159
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    • 2006
  • The pine mushroom fruit body have a harvest only from natural pine forest instead of artificial culture like other edible mushrooms until now on. We investigated the interrelationship between pine mushroom colonies and insect/invertebrates fauna, and changes of their seasonal populations. Two famous pine mushroom producing district were selected on Bong-wha and Gan-sung Eup, and were surveyed on the ground and in the soil with/without pine mushroom hyphae per month from June to November, 2005. There was some difference in collected Insects and invertebrates between two producing districts. Total number of collected species and individuals were 73 and 22, and 63 individuals with 19 species in Bon-wha and Gan-sung area, respectively. Otherwise, there were many mites and nematodes were living in soil with mushroom hyphae. The population densities of mites were Increased to November from June, however, the population of nematodes were fluctuated with low in hot weather and high in cool.

A study on the Analysis Method and Pollution Measure of existed VOCs during the Bulk Liquid Cargo Operation at Vessels (산적 액체 화물 작업중 배출되는 VOCs 분석방법 및 오염 측정 연구)

  • Cheong, Kwang-Hyun;Lee, In-Ho
    • Proceedings of KOSOMES biannual meeting
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    • 2007.05a
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    • pp.21-26
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    • 2007
  • The Protocol adopted in Sep. 1997 included the new Annex VI of MARPOL 73/78, which entered into force on 19 May 2005. Revision for the accept MARPOL Annex VI with make public on 29 Dec. 2005 and enforce on 29 Jun. 2006. For all that not installation at oil terminal or if installed do not used. The purpose of this study is to research and evaluate the methods of sampling and analysis, and the pollution measure of existed VOCs during the bulk liquid cargo operation at vessels. As the sampling instrument of VOCs, Tedlar bag has the effect of decrease in concentration by the chemical reaction and absorption, and the method using the solvent extraction has error cause because of the extraction, the absorption tube has error by the breakthrough. But canister is the best sampling instrument because of having no sampling mistake. The study on VOCs emission is not sufficient for the research on the pollution cause and the analysis data on the HAP during the bulk liquid cargo operation at vessels. Hence, monitoring on the management hazardous VOCs need to be continued during the cargo operation at vessels and VOCs management field must be study for the make progress at a good pace for reason of crew's health.

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The Cytoskeletal and Chromosomal Constitution of Vitrified Immature Mouse Oocytes (초자화동결된 생쥐 미성숙란의 세포골격과 염색체성상)

  • Park, Se-Pill;Yi, Bong-Kyung;Kim, Eun-Young;Nam, Hwa-Kyung;Lee, Keum-Sil;Yoon, San-Hyun;Chun, Kil-Saeng;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.3
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    • pp.363-368
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    • 1999
  • This study was to confirm whether the vitrification method using EFS40 freezing solution has detrimental effect on the cytoskeleton and chromosome constitution of the immature mouse oocytes by indirect immunocytochemistry and chromosome analysis. Immature mouse oocytes were vitrified using EFS40 (40% EG, 18% ficoll, 0.5 M sucrose diluted in M2 medium), thawed and then survived oocytes were in vitro matured for 16 hr. When the microtubule morphology and micro filament distribution in vitrified-thawed immature mouse oocytes were examined, normal percentage of two cytoskeleton in vitrified group (93.9 and 100.0%) was not significantly different from that in control (100.0 and 100.0%) and exposed group (94.4 and 100.0%). The rate of oocytes containing a normal chromosome number in vitrified group was 65.8%, this result was not significantly different from that in control (79.6%) and exposed group (69.0%). These results indicated that exposure to cryoprotectant or freezing has not effect on the alteration of cytoskeleton morphology and the chromosome constitution of mouse oocytes and that our vitrification methods using EFS40 freezing solution was suitable for the cryopreservation of immature mouse oocytes.

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Communication overhead management techniques based on frequency of convergence contents using the P2P environment (P2P 환경에서 융복합 컨텐츠 사용 빈도수에 따른 통신 오버헤드 관리 기법)

  • Han, Kun-Hee;Jeong, Yoon-Su
    • Journal of Digital Convergence
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    • v.13 no.5
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    • pp.245-250
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    • 2015
  • The P2P technology is becoming a technology for receiving a service, such as the latest YouTube and Facebook. P2P technology can be easily downloaded to a large amount of multimedia content over a distributed environment. However, pictures, music, a large amount of multimedia content such as videos each user is unable to manage the content management operating smoothly because, Song communication processing overhead caused when the reception is likely to remain the problem. In this paper, we propose a convergence content management technique that provides a low communication overhead of the frequency of use of the convergence content when trying to provide a large amount of convergence content to users in a distributed environment. Our method can be used to select the appropriate frequency is high among the shared content on the P2P multimedia environments that are distributed across a frequency of use of the convergence content and provides the service. Experimental evaluation proposed method, the convergence content browsing speed than conventional techniques improved communication about 13.3% and reduced overhead to about 9.5%.

Quality Characteristics and Antioxidant Activities of Macaron with Opuntia ficus-indica var. saboten Powder (백년초 분말을 첨가한 마카롱의 품질 특성 및 항산화 활성)

  • Kim, Sook-Young;Han, Gi Dong;Jung, In-Chang;Kim, Ki-Ju
    • Journal of the East Asian Society of Dietary Life
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    • v.27 no.3
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    • pp.332-340
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    • 2017
  • The aim of this study was to evaluate the quality characteristics, antioxidant activities, and sensory properties of macaron with Opuntia ficus-indica var. saboten powder (OP). Physicochemical and sensory properties of macaron with different amounts (0%, 2%, 4%, and 6%) of OP were examined. The quality of macarons was evaluated based on spread factor, baking loss rate, color, texture, antioxidant activities, and sensory evaluation. As amount of OP addition increased, spread factor and a value increased, whereas baking loss rate, and L and b values decreased (p<0.05). For texture properties, hardness, springiness, and brittleness were lower as OP content increased while cohesiveness and gumminess were not significantly different (p<0.05). For total polyphenolic contents, DPPH and reducing power significantly increased upon addition of OP at high concentrations (p<0.05). In the sensory evaluation of macarons, scores for color, flavor, taste, texture, and overall acceptance were highest with 4% OP, whereas macaron containing 6% OP showed the lowest scores for taste and texture (p<0.05). These data suggest that addition of 4% OP is the optimal concentration for making macaron.

Effects of Activated Charcoal on in vitro Ruminal Fermentation Characteristics and Nutrient Disappearances (사료에 대한 활성탄의 첨가가 in vitro 시험시의 발효성상 및 영양소 소실율에 미치는 영향)

  • Lee, B.D.;Lee, S.K.;Lee, K.D.
    • Korean Journal of Agricultural Science
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    • v.26 no.2
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    • pp.25-32
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    • 1999
  • An in vitro study was conducted to examine the effects of the addition of activated charcoal (AC) on the ruminal fermentation characteristics, nutrient disappearance, and ruminal gas production. AC was added at the levels of 0.00, 0.25, and 0.50 % to each of the four types of diets (roughage/concentrate ratio : 8/2, 6/4, 4/6 and 2/8), respectively. Although not significant, ruminal pH tended to increase by adding AC, and as the concentrate level increased, ruminal pH decreased (P<0.05). Acetate concentration and acetate/propionate molar ratio tended to decrease in AC diets. but molar % of propionate tended to increase by the addition of AC. Ruminal degradation of dry matter, crude protein, NDF, ADF, and hemicellulose in AC diets tended to increase than in non-AC diet, however, no tendency in ruminal degradation of crude fat was observed. As the concentrate level increased, rumunal degradation of dry matter and nutrients in AC diets increased significantly(P<0.05). Ruminal gas production tended to decrease in the 0.50 % AC diets, however, it tended to increase in high roughage diets. Although there appeared some beneficial effects in adding AC to ruminant diets in this study, more works should be done with AC before we can make clear conclusion on the use of AC in the ruminant diets.

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Influence of Water Temperature on Growth of Yearling Sea Bass, Lateolabrax japonicus in Indoor Tank (농어, Lateolabrax japonicus 유어의 성장에 있어 사육 수온의 영향)

  • 강덕영;한형균;전창영
    • Journal of Aquaculture
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    • v.17 no.4
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    • pp.240-245
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    • 2004
  • Two experiments were conducted to study the effect of temperature on growth and survival of yearling sea bass, Lateolabrax japonicus reared from 3$^{\circ}C$ to 33$^{\circ}C$. In the I st experiment, we used yearlings fish averaging 19.2$\pm$0.2 cm/fish (Mean$\pm$S.E.M.) of total length (TL) and 67.0$\pm$1.8 g/fish (Mean$\pm$S.E.M.) of body weight (BW), and we cultured the fish at 3, 6, 9, 12 and 17$^{\circ}C$ for 90 days. In the 2nd experiment, the experimental fish averaging 24.9$\pm$0.1 cm/fish (Mean$\pm$S.E.M) of TL and I 46.4$\pm$3.0 g/fish (Mean$\pm$S.E.M) of BW were reared at 21,24,27,30 and 33$^{\circ}C$ for 90 days. During these experiments, we measured food intake, feed efficiency, survival and growth (TL and BW) in the both experiments. Although food intake of the yearling increased with the temperature, the feed efficiency was only enhanced within the temperature range, from 21 to 27$^{\circ}C$. Growth of yearling was normal within the temperature range from 17 to 3$0^{\circ}C$, but it was stoped or reduced in other temperatures. Survival rate was significantly reduced in 3$^{\circ}C$ from the 1st experiment and in 30 and 33$^{\circ}C$ from the 2nd experiment, but there was no significant difference among other groups.

Biological Potential of Enzymatic and Polyphenol Extracts from Ecklonia cava (감태 효소 추출물 및 폴리페놀 추출물의 생리활성에 관한 연구)

  • Lee, Su Min;Kim, Jin Eun;Oh, Myoung Jin;Lee, Joo Dong;Jeon, You-Jin;Kim, Bora
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.39 no.1
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    • pp.19-24
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    • 2013
  • To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. cava ($SC_{50}$ 42.9 ppm) and AG-DK ($SC_{50}$ 6.4 ppm) showed a strong DPPH free radical scavenging activity. The anti-glycation ability of the enzymatic extract of E. cava and AG-DK was tested using bovine serum albumin (BSA), which inhibited the formation of advanced glycation end-products (AGEs) in the BSA/glucose system. The enzymatic extract of E. cava ($IC_{50}$ 97.2 ppm) and AG-DK ($IC_{50}$ 7 ppm) had inhibitory effects on tyrosinase activity. Moreover, the enzymatic extract of E. cava and AG-DK had an anti-inflammatory effect through the inhibition of nitricoxide (NO) and prostaglandin E2 ($PGE_2$). These findings suggest that the enzymatic extract of E. cava and AG-DK can be applied to skin-care products as cosmetic ingredients.

Optimization of Extraction Condition on Fig (Ficus carica L.) by Response Surface Methodology (반응표면분석법에 의한 무화과 열수 추출조건의 최적화)

  • Kim, Jung-Ok;Kwon, Soon-Tae;Lee, Gee-Dong;Hong, Joo-Heon;Moon, Doo-Hwan;Kim, Tae-Wan;Kim, Dae-Ik
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.66-73
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    • 2008
  • Response surface methodology (RSM) was applied for monitor the yields of desirable substances from fig (Ficus carica L) under different extraction conditions. The maximum yield was 66.46% at 22.08 mL/g of solvent to sample ratio, $90.59^{\circ}C$ extraction temperature and 148.04 min extraction time. The maximum total phenolics was $121.31{\mu}g/mL$ at 17.87 mL/g, $98.82^{\circ}C$, and 130.80 min. The maximum electron donating ability was 54.09% at $121.31{\mu}g/mL$, 18.13 mL/g, and $98.81^{\circ}C$. The maximum value of protease activity was 54.51 unit/min at 17.45 mL/g, $99.01^{\circ}C$, and 131.43 min. In addition, the maximum value of reducing sugar content was 19.14 mg/mL in 22.66 mL/g, $86.30^{\circ}C$, and 153.59 min. The optimum conditions estimated by RSM for maximal extraction of the effective components were $17{\sim}25$ mL/g of solvent to sample ratio, $80{\sim}100^{\circ}C$ of extraction temperature, and $100{\sim}170$ min of extraction time.

Studies on the Submerged Culture of Lentinula edodes Mycelia in Brewer's Yeast Extract Medium (맥주효모 추출물을 이용한 표고버섯 균사체의 심부배양에 관한 연구)

  • Lee, Jae-Yun;An, Won-Gun;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.22 no.3
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    • pp.266-275
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    • 1994
  • Brewer's yeast extract can be used as a good substrate for the culture of Lentinula edodes Mycelia(LEM). We found that it was better to filter the extract through three kinds of sieves and then to heat for hydrolysis and concentration at $90^{\circ}C$ prior to use. Also the maximum condition for the growth of LEM was investigated. We found that addition of inorganic salts such as calcium enhanced the growth of LEM. On the other hand, addition of carbon and nitrogen sources to the medium did not affect, and even inhibited under certain conditions, the growth of LEM. The maximum temperature for the growth of LEM was around $25^{\circ}C$. Also, it grows better when agitated by shaking at 100 rpm for airration. The appropriate concentration of the extract to use was 10%. Under these conditions, LEM could reach to the confluency after cultivation of 12 days. Our extract formula seems better than other available media for LEM growth, producing higher crude protein content and better taste.

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