• Korean Journal of Environment and Ecology
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• v.8 no.1
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• pp.74-77
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• 1994

To examine the effects of gibberelin treatment on seed germination of five Viola species - Viola variegata, Viola dissecta var, chaerophylloides, Viola collina, Viola hirtipes and Viola manshrica - in Wonju district, pretreated seeds of five species were sowed on Jiffy strips with mixed soil(sand： peat moss： perlite： vermiculite, 1:1:1:1, v/v) in 25, May 1994. Seeds were soaked in 5, 25, 50ppm gibberelin solution and ground water during 24 hours. Seed germination rate was measured and compared among species and treatments. Seed germination rate of Viola hirtipes was differed higly significant among pretreatments, but those of other species did not significant at 5of level. In general GA$_3$pretreatment raised seed germination rate of 5 Viola species, and proper concentration of GA$_3$was 25~50ppm.

• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.56-61
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• 1995

To examine the effects of gibberelin treatment on seed germination of six wild herbaceous species -Dicentra spectabilis, Aster pekinensis, Agastache rugosa, Elsoholtzia ciliata, Boehmeria tricuspis and Pulsatillia cerrma var. chaerophyiloides - in Kangwon-do, pretreated seeds of five species were sowed on plastic pots with mixed soil(sand：peat moss：perlite： vermiculite, 1：1：1：1, v/v) in 23,April 1995. Seeds were soaked in 25,50 ppm gibberelin solution and ground water during 24 hours. Pretreated seeds of Pulsatiilia cerrma var. chaerophylloides were sowed on nursery soil in 1, June 1995. Seed pretreatments in 31, May 1995 were same method. Seed germination rate was measured and compared among species and treatments. Seed germination rate of Dicentra spectabilis, Agastache rugosa and Elsoholtzia ciliata was differed higly significant among pretreatments ,but those of that of Aster pekinensis, Boehmeria tricuspis and Pulsatillia cerrma var chaerophylloides did not significant at 5% level. Gibberelin pretreatment raised seed germination rate of Dicentra spectabilis and Agastaohe rugosa and proper concentration of GA$_3$ was 25ppm and seed germination rate of Elsoholtzia ciliata was highest at GA$_3$ 50ppm plot. The utilization of wild herbaceous species were discussed.

• Asian Journal of Turfgrass Science
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• v.3 no.2
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• pp.65-72
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• 1989

Tes ratablish a liawn Zoysia' Japonica Steud. with seeds. a experiment was conducted for the invrati ignt ic in oil seed's physic sit ogical maturity. The rt.sultt an' Summarized as follows: 1. The point of maximum weight off 1000 seeds and 100 seedling, germination speed and germination percentage were reached at 30 day. after anthesis. it would be considered as physiological maturity of Zoysia japonica seed. 2. The seeds moistures contents at physiological maturity was decreased in 18%. 3. Germination vigor deteriorated prior to viability. 4. The seeds were capable of germination in 3:4% at 10 days after anthesis. 5. Field deterioration of seeds occurer from 40 (lays after anthesis to 60 days and sprouted seedilng vigor was very poor.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.285-290
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• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.461-471
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• 2016

This study was conducted to establish an in vitro propagation system for sea-milkwort (Glaux maritima L.), which is an endangered coastal plant species with high horticultural value. Two phenotypes, 'Red type (RT)' and 'Pistachio type (PT)' based on the colors of stem and flower, were obtained from a personal horticulturist in 2009 and used for this study as plant materials. The stock plants showed typical morphologies in flower, capsule, and seed appearances as previously reported. Low temperature treatment at $4^{\circ}C$ for four or more weeks after in vitro sowing maximized seed germination percentage, indicating that imbibition of seed and subsequent low temperature treatment are crucial for its germination. The in vitro seedlings had phenotypic variation, falling into 'RT' and 'PT' classes like the stock plants. Although slight differences depending on genotype and medium were recognized, the fourth or fifth nodes detached from the in vitro seedlings revealed the best multiplication efficacy when estimated on the basis of total number of nodes of newly developed axillary shoots. In addition, the nodes from 'RT' and 'PT' regenerated the most shoots on medium supplemented with $0.5mg{\cdot}L^{-1}$ BA alone and $0.5mg{\cdot}L^{-1}$ BA plus $0.5mg{\cdot}L^{-1}$ IAA, respectively. The node culture-derived plantlets were well acclimatized in a culture room ex vitro and completed the pseudo-annual life cycle coincident with that in the natural salt march habitat with the current cultivation method of applying fresh water-irrigation under an inland environment. This work represents the first report of in vitro propagation of sea-milkwort. Thus, our study will contribute to exo-habitat conservation and natural habitat restoration of this endangered species in addition to development of a horticultural product.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.111-115
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• 2009

The seeds of Phellodendron amurense have difficulty to obtain because these plants show dioecism with independent distributions. This experiment was conducted to find the effects of soaking and prechilling treatment on the germination of P. amurense seeds. Seeds were soaked for 3, 5 and 10 days (soaking treatment), and placed on petri-dish at $4^{\circ}C$ for 10, 20, 30 and 50 days after soaked at room temperature for 24 hours (prechilling treatment). After the treatments, germination percentage, mean germination time, germination speed and germination performance index were analyzed. As a result of sowing, prechilled seeds for 30 days had the highest percent of germination, whereas all of the soaked seeds showed lower percent of germination than non-treatment (control). In case of mean germination time, only prechilling for 30 days was more effective than control. In germination speed, all of the soaked seeds had lower values than control whereas all prechilled seeds except prechilled seeds for 20 days showed higher values than control. Especially prechilled seeds for 30 days showed the highest rate among the prechilling treatments. Germination performance index was similar to germination speed.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.385-392
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• 2016

This research was performed to develop seminal propagation method of Saururus chinensis (Lour.) Baill. by conducting a rigorous germination study. Well-selected seeds were dry-stored at 4 ± 1.0℃ during the experiment. To study dormancy type, non-stored seeds were analyzed by embryo observation, germination test and detecting for any difficulties in seed coat to absorb moisture. Then to improve germination, seeds were submerged for 24 hours in a solution of varying concentrations containing one of plant growth regulators and minerals. According to research, fleshly matured seeds had an undifferentiated embryo and had a low germination rate below 5%. In addition, water submersion led to moisture absorption, embryo in the seeds grew and germinated so it was deemed morphophysiological dormant seeds. Percent germination (PG) and germination energy (GE) was greatly improved by soaking in plant growth regulators and minerals for 24 hours. Especially, 500 ㎎/L GA₃ treatment resulted in the highest GE as 46.1%. KNO₃ meaningfully improved PG (54.3∼57.7%) at 10∼20 mM but effect of minerals on germination acceleration as GE were negatively impacted in all concentrations.

• FLOWER RESEARCH JOURNAL
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• v.18 no.2
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• pp.142-146
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• 2010

'Arirang', an early-flowering pure white gladiolus with purple core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 1998. The selection was derived from the cross between the progenies of the early flowering dark orange 'Arianne' and bright red domestic cultivars 'Hongkwang'. The breeding was conducted in 1993 and finally selected in 1998 after five (5) years (1994 to 1998) of experimentation. 'Arirang' is a cut flower with bright white color petals and purple core which exhibited early flowering and vigor without stem bending. These can be cultivated in areas or regions with strong winds during summer. 'Arirang' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant had high tolerance to flooding and resistance hence could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Arirang' had a 10.5 cm wide flower and 140 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Arirang'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

• Korean Journal of Medicinal Crop Science
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• v.6 no.4
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• pp.258-264
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• 1998

This study was conducted to establish mass propagation system from seeds of Lavandula angustiolia Mill. Only 4% of dry seeds were germinated when they were incubated in $4^{\circ}C$. Germination rate of stratified seeds for 9 weeks was 15% higher in light than darkness. Soaking with 1000mg/l of $GA_3$ was helpful to overcome the effect of darkness. The optimal temperature for germination was $25^{\circ}C$ for the seeds that were treated with$GA_3$, solution. For the improvement of germination rates, pretreatment of $GA_3$ at 500-2000mg/l showed about 75% of germination, and in the combination treatment of $GA_3$, and BA, germination rate increased by about 10% in the treatment of 1000mg/l $GA_3+10mg/l$ BA compared with the 1000mg/l $GA_3$ treatment. Cold stratification treatment was very effective for seed germination, and over 70% of seeds were germinated when they were incubated in $4^{\circ}C$ for 9-12 weeks. In addition, there was synergic effect on the seed germination subject to stratification and 1000mg/l $GA_3$, treatment for 6 months. In the mixture of vermiculite: peatmoss (1 : 1, v/v), emergence rate was 6.7% in control and 65% in 1000mg/l $GA_3$ treatment, respectively.

• Journal of Korean Society of Forest Science
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• v.30 no.1
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• pp.50-60
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• 1976

For finding out the proper method of developing Buxus koreana var. insularis, this study was carried out in Pokil-Do, Wan-Do, Chonnam, in 1975. The ecology, morphology and characteristics of the plant were mainly investigated and analyzed. The results are summarized as follows; 1. The small islands of the southern sea of Korea; Pokil-Do, Chin-Do and So-Huk-San-Do, turn out to be the site of the natural communities of B. koreana var. insularis. 2. The growth of the plant is fairly good in the acid soil of these islands. 3. This vegetation of composed as the three-layered communities, these communities consit of Camellia, Buxus and Cares. 4. The plant is a board leaved evergreen shrub that is tolerant to shade and native to the temperate zone. 5. The size of the leaves, fruits and seeds is slightly larger than those of B. koreans. 6. As fruiting and germination of the seeds are favorable. it is easy for us to make a number of seedlings. 7. The proper times for sowing and harvesting the seeds are around the middle of July and August, respectively. 8. The seedlings is difficult to grow in the naked ground because the germinated seeds are seriously damaged by frost. 9. The plant growth per annum is about 8 to 10cm in height, 1.5mm in diameter. It is graceful looking as the branches and leaves as of the dense foliage type 10. It is possible to develop the plant in many ways, such as; ornamental plant, bee plant, sculptural and industrial materials.