• Korean Journal of Environment and Ecology
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• v.6 no.1
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• pp.9-11
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• 1992

To examine aluminum toxicity on woody plants, Forsythia koreana cuttings were grown in the aluminum solution and ground water(pH 6.75). Aluminum solution were prepared 1.0, 2.5 and 5.0mM aluminum potassium sulfate, dilulted with ground water. Shoot growth, leaf number, leaf injury and leaf chlorophyll content were measured and compared among the treatments. In all growth-related characters(shoot growth. leaf number, leaf injury and leaf chlorophyll content), differences among the treatments were highly significant. Forsythia koreana cuttings were severely stressed in aluminum solution more than 1.0mM concentration.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.117-122
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• 2000

This study was carried out to know the factors affecting on shoot formation and rooting for stable and routine production of plantlets in bioreactor culture of Scrophularia buergeriana. Multiple shoots were formed effectively when explants were transplanted on the MS media with decreased concentration of $NH_4NO_3$ as 413mg/ l . Three hundred stem explants (0.8-1.0cm) was appeared as proper inoculation size in bioreactor culture. IBA (0.05mg/L) was more effective for rooting of the shoots in liquid as well as solid media. Six weeks long culture of explants in bioreactor gave better shoot shape for rooting on solid half-strength MS media.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.263-269
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• 2007

To establish a optimum culture condition in vitro for production of seedlings in Wasabia japonica, the effects of salt strength, sucrose concentration, and $NH_4/NO_3$ ratio on the shoot growth in MS medium were investigated. The full strength of MS medium was the best condition for shoot growth, and in $1/4{\sim}1/2x$ or 2x MS medium the growth was significantly suppressed. Growth of shoots was enhanced when sucrose was added in MS medium after 2 weeks of culture, and the best result was found at 3% sucrose addition. ${NH_4}^+/{NO_3}^-$ ratio of 10:50 gave the highest biomass of shoot in the liquid culture with MS medium, and increasing the ${NH_4}^+$ ratio in the medium significantly reduced the growth of shoot.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.281-285
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• 2005

To establish the mass proliferation system of Ardisia pusilla DC, the shoot tips of Ardisia pusilla DC were cultured on the MS and half-strength MS medium supplemented with $0{\sim}5.0$ mg/L BA or $0{\sim}0.5$ mg/L thidiazuron(TDZ), respectively. A few multiple shoot formation observed when the shoots were cultured on MS medium containing TDZ. However, the frequency of multiple shoot formation was reached up to 82.4%, when the shoots were cultured on half-strength MS medium supplemented with 0.5 mg/L BA. Also the number of shoot per explant was 7.1. To promote rooting from multiple shoot, newly formed shoots were transferred to half-strength MS medium containing 0.5 mg/L IBA or 0.5 mg/L NAA, respectively. Regenerated plantlets were grown to normal mature plants in soil.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.15-23
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• 2001

Effects of growth regulators, scion length and scion diameter on shooting and seedling characteristics were investigated to increase seedling products by root cuttings of lacquer tree. Shoots developed and emerged above-ground from the root scion at 3∼5 weeks after cut plating, most of that was a shoot but some of that were 2∼3 shoots. Otherwise, non-shooting was the reason that cortical layer was decayed. Soaking in zeatin was the most effective on shooting of root cuttings and high concentration was effective. Also soaking on high contents of BA or kinetin was effective on shooting but was lower totally than soaking on zeatin. Seedling characteristics among 3 growth regulators and 3 concentrations were not any difference in statistical significant, but were move excellent than control. As root scion was tall, shoots were developed at early. 15cm length scion was the most excellent to use seedlings but 3cm length scion was wickedness. 10cm scion, however, was highest to product of seedlings than any others. 0.3∼0.5 cm length scion was showed 76% shooting rate at 10 weeks after cut planting, but seedling characteristics were not any difference among the kind of 3 diameters.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.310-317
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• 2019

Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica 'Fuji') leaf explants were developed in the present study. The effects of different basal media, types and concentrations of carbon sources, and concentrations of plant growth regulators were evaluated to determine the optimal shoot regeneration conditions for 'Fuji' apple leaf explants. On different treatments involving combinations of basal media, LS and N6 media, and different types and concentrations of cytokinins, 6-benzyl-adenine (BA) and thidiazuron (TDZ), shoot regeneration rates were the highest in the N6 medium combined with BA. Among the plant growth regulator and carbon source combination treatments, 5.0 mg/L BA, and 0.1 mg/L α-naphthalene acetic acid (NAA) with 40 g/L sorbitol was the optimal combination for shoot regeneration. In addition, the optimal sorbitol concentrations for shoot regeneration were 40 g/L and 60 g/L. The highest regeneration (81.8%) was achieved using 40 g/L sorbitol. The regenerated shoots elongated and rooted on rooting medium, consisting of 1/4 MS medium with 0.2 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and the regenerated plants exhibited normal phenotypes.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.101-104
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• 1995

The experiment was conducted to identify the optimal in vitro propagation condition for P. lactiflora Pall. Through apical shoot tip and axillary shoot tip culture of winter bud. When apical shoot tip and axillary shoot tips excised from winter bud were cultured on MS medium supplemented with various concentrations of plant growth regulators, all the apical shoot tips elongated regardless of the composition of the medium but axillary shoot tips responded differently. Shoot of 'Uisong' local cultivate was well elongated in the medium containing 0.01mg/L NAA. Frequency of shoot formation and subsequent shoot growth in axillary shoot tip culture were promoted in the medium containing 0.01 mg/L NAA and 5.0mg/L zeatin. 30% of the elongated shoots were vigorously rooted on the medium containing 0.1mg/L NAA with vermiculite as a support medium.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.63-69
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• 2000

In order to establish a micropropagation system for buffalo gourd (Cucurbita foetidissima ) and common milkweed (Asclepias syriaca), the effects of several plant growth regulators and culture temperature on shoot multiplication and rooting were investigated. In buffalo gourd, the greatest number of shoot from shoot tip culture and well growth of formed shoot were obtained on the MIS medium supplemented with 1.0 mg/L BA and 0.3 or 0.6 mg/L IAA. Whereas kinetin and 2iP were not effective for shoot multiplication in vitro. It was found that 22$^{\circ}C$ and $25^{\circ}C$ were suitable for shoot multiplication. Roots were easily formed by the addition of auxins, especially 1.0 or 2.0 mg/L IBA and 2.0 mg/L IAA. Over 90% of plants survived successfully after being transferred into the field. In common milkweed, BA was more effective than kinetin or 2iP for its micropropagation in vitro. The increased shoot weight and number of nodes per shoot were obtained on the medium containing 3.0 mg/L BA and 0.3 or 0.6 mg/L IAA. But 2iP promoted the shoot elongation. In addition. common milkweed was sensitive to culture temperature in vitro. Temperature around 22$^{\circ}C$ was favorable for shoot multiplication and growth, whereas temperature higher than $25^{\circ}C$ usually reduced the rate of shoot survival rate.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.270-277
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• 2021

Veronica rotunda var. subintegra (Nakai) T. Yamaz. is perennial herb belonging to the family Scrophulariaceae, native to Korea. Plant growth regulators, cutting mediums, cutting position, and leaf size were examined to investigate the requirements for cutting propagation of V. rotunda var. subintegra. This study was conducted over a period of 6 weeks from March to May 2017 in the Division of Plant Resources, Korea National Arboretum. For all treatments, survival and rooting percentage was 100%. In PGRs experiment, the number of roots and shoot length treated with 100 mg/L IBA treatment was significantly higher than that of NAA treatment. The shooting percentage was decreased with an increase of the PGRs concentration. In growing substrates experiment, the number of roots on commercial soil (61.2) was the highest, followed commercial + kanuma soil (45.7), and the lowest was kanuma soil (41.8). The shoot length treated with commercial soil (8.9 cm) was significantly greater than that of kanuma soil (4.1 cm). In cutting positions experiment, the number of roots treated with apical (56.6) was significantly higher than that of basal (38.8). In leaf-cut experiment, the number of roots and of shoot length treated with full leaf was significantly higher than that of half leaf. As a result, we recommend treating cuttings with 100 mg/L IBA treatment, commercial soil, apical position, and full leaf for successful cutting propagation of V. rotunda var. subintegra.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.407-412
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• 1997

Allelopathic activity of methanol extract from medicinal plants was determined based on the inhibition of germination, shoot and root length of Echinochloa crus-galli. The response was depended on the source of extracts and concentrations. Of the 20 test plants tested, Cnidium officinale showed most inhibition according to germination, shoot and root length of E. crus-galli. Based on the plant parts, the root extract of C. officinale had more inhibitory effect on E. crus-galli than the stem+leaf extract. Phytotoxic effects of the root extract on E. crus-galli showed 75% inhibition of seed germination, 95% inhibition of shoot growth, and 100% inhibition of root growth at the concentration of 1g/petridish. Sequential partitioning of root extract with organic solvents with increasing polarity showed that the ethylacetate fraction had the greatest inhibitory effect on E. crus-galli. Germination was not inhibited at any rate of ethylacetate fraction but shoot and root growth, were significantly inhibited even at 2mg/petridish. This indicates that the most toxic phytotoxin present in the root extract may be moderate polar compound.