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http://dx.doi.org/10.5010/JPB.2005.32.4.281

In vitro Mass Propagation of Ardisia pusilla DC.  

Kang Gwan-Ho (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University)
Oh Owel-Sun (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University)
Goo Dae-Hoe (National Horticultural Research Institute, RDA)
Eun Jong-Seon (College of Agriculture & Life Sciences, Chonbuk National University)
Kim Hyung-Moo (College of Agriculture & Life Sciences, Chonbuk National University)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 281-285 More about this Journal
Abstract
To establish the mass proliferation system of Ardisia pusilla DC, the shoot tips of Ardisia pusilla DC were cultured on the MS and half-strength MS medium supplemented with $0{\sim}5.0$ mg/L BA or $0{\sim}0.5$ mg/L thidiazuron(TDZ), respectively. A few multiple shoot formation observed when the shoots were cultured on MS medium containing TDZ. However, the frequency of multiple shoot formation was reached up to 82.4%, when the shoots were cultured on half-strength MS medium supplemented with 0.5 mg/L BA. Also the number of shoot per explant was 7.1. To promote rooting from multiple shoot, newly formed shoots were transferred to half-strength MS medium containing 0.5 mg/L IBA or 0.5 mg/L NAA, respectively. Regenerated plantlets were grown to normal mature plants in soil.
Keywords
Ardisia pusilla; BA; micropropagation; shoot tip; thidiazuron;
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Times Cited By KSCI : 4  (Citation Analysis)
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