• Title/Summary/Keyword: 식물대사

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Screening of Edible Mushrooms for the Production of Lovastatin and its HMG-CoA Reductase Inhibitory Activity (Lovastatin을 생산하는 식용버섯 선발과 HMG-CoA reductase 저해 효과)

  • Lee Jae-Won;Lee Soo-Min;Gwak Ki-Seob;Lee Ji-Yoon;Choi In-Gyu
    • Korean Journal of Microbiology
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    • v.42 no.2
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    • pp.83-88
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    • 2006
  • This research was performed to determine the production of lovastatin and its HMG-CoA reductase activity produced by fruit bodies and mycelial liquid cultures of domestic edible mushrooms (8 fungal strains). By deter-mining TLC analysis for the confirmation of the presence of lovastatin, all the extracts from fruit bodies and mycelial liquid culture showed same Rf value (0.46), whick was identical to that of the standard lovastatin. In order to extract lovastatin from fruit body, the mixture of water/acetonitrile/methanol was chosen as the most effective solvent. Extracts from fruit body and mycelial liquid culture of pleurotus ostreatus produced the high-est lovastatin 0.98 mg/g based on dry biomass, and 21.90 mg/L, respectively. In the inhibition rate of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, the highest was obtained in P. ostreatus as 67.8% among fruit bodies, and the rates of mycelial liquid culture extracts from P. ostreatus and Laetiporus sulphureus were 37.2% and 29.1%, respectively. Unusually L. sulphureus showed high inhibition rate with low content of lovastatin due to the contribution of campesterol and gamma-sitosterol with hypocholesterolemic activity as metabolites.

Growth and Quality Changes of Creeping Bentgrass by Application of Keratin Amino Acid Fertilizer (케라틴 아미노산 비료 시비에 따른 크리핑 벤트그래스의 생육과 품질 변화)

  • Jo, Gi-Woong;Kim, Young-Sun;Ham, Soun-Kyu;Lee, Jae-Pil;Kim, Doo-Hwan;Kim, Woo-Sung;Lee, Geung-Joo
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.260-267
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    • 2016
  • Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.

Environmental Characteristics and Vegetation of Hanabusaya asiatica Habitats (금강초롱꽃 자생지의 환경특성과 식생)

  • Jang, Su-Kil;Cheon, Kyeong-Sik;Jeong, Ji-Hee;Kim, Zin-Suh;Yoo, Ki-Oug
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.497-506
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    • 2010
  • This study intended to investigate the environmental factors including soil and vegetation in order to better understand the environmental and ecological characteristics of ten different habitats of $Hanabusaya$ $asiatica$. These habitats, according to investigations, are mostly located on the slopes of mountains facing north at an altitude of 580 m to 1,396 m above sea level with angles of inclination ranging from 5 degrees to 80 degrees. A total of 146 vascular plant taxa are identified in 32 quadrates of the ten habitats. The importance value of $H.$ $asiatica$ is 8.87%, and 5 highly ranked species such as $Carex$ $siderosticta$ (8.67%), $Ainsliaea$ $acerifolia$ var. $subapoda$ (7.10%), $Calamagrostis$ $arundinacea$ (6.79%), $Athyrium$ $yokoscense$ (5.33%), $Astilbe$ $rubra$ (3.11%) are considered to be an affinity with $H.$ $asiatica$ in their habitats. Dominant species of woody plants in ten habitats are represented as $Quercus$ $mongolica$ in tree layer (T1), $Acer$ $pseudo$-$sieboldianum$ in subtree layer (T2), $Rhododendron$ $schlippenbachii$ and $Tripterygium$ $regelii$ in shrub layer (S). The degree of their average species diversity is 1.30, and that of dominance and evenness are 0.08 and 0.88, respectively. The type of soil is sandy loam, loam and loamy sand, and the average field capacity of soil is 23.95%. Their average organic matter is 12.28%, soil pH 5.79, and available phosphorus is 25.48%. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of tree layers is correlated with richness, diversity, dominance, evenness and coverage of $H.$ $asiatica$.

Antioxidant and Alcohol Dehydrogenase Activity of Water Extracts From Abalone Containing Medicinal Plants (전복과 천연 식물류 복합물의 항산화 및 알코올대사 효소 활성)

  • Shin, Jung-Hye;Lee, Soo-Jung;Choi, Duk-Ju;Kang, Min-Jung;Sung, Nak-Ju
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.182-187
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    • 2008
  • This study was performed to evaluate the possible production of a functional beverage derived from abalone and with several plants (AP). Five types of AP composites were prepared having different medicinal plant compositions (AP- I : abalone, garlic, jujube, Chinese matrimony fruit, dried orange peel, licorice root, zinger, honey; AP- II: abalone, garlic, black bean, Chinese matrimony fruit, cornus fruit, licorice root, zinger, honey; AP-III : abalone, jujube, arrow root, milk vetch, dried orange peel, licorice root, zinger, honey; AP-N: abalone, black bean, arrow root, milk vetch, cornus fruit, liquorice root, zinger, honey; AP-V : abalone, garlic, Chinese matrimony fruit, milk vetch, licorice root, zinger, honey). In vitro analysis were performed to examine the antioxidant contents and alcohol dehydrogenase activities of the composites. AP- II had the highest total phenol contents ($28.55{\pm}1.56\;mg/l00\;g$), and AP- V the highest level of flavonoids ($47.61{\pm}1.58\;mg/l00\;g$). At $78.89{\pm}0.16%$, AP- V displayed the strongest electron donating ability followed by AP-II($57.99{\pm}0.21%$) and API ($37.66{\pm}0.20%$). Reducing power was also significantly higher in AP- V. The Hydroxyl radical scavenging and SOD-like activities of all composites were less than 15% and 20%, respectively. At 12.5% alcohol concentration, ADH activity ranged from $114.47{\pm}2.18{\sim}121.39{\pm}4.36%$ and ALDH activity ranged from $100.04{\pm}2.90{\sim}129.54{\pm}4.80%$; AP- I , AP- II, and AP- Vin 12.5% of alcoholic concentration. The composites of AP- I , AP- II, and AP- V, all containing garlic and Chinese matrimony fruit, were significantly stronger than AP-III and AP-N. Finally, also at 12.5% alcoholic concentration, the ALDH activity of AP- V was higher than its ADH activity.

Development of the conventional crop composition database for new genetically engineered crop safety assessment (새로운 생명공학작물 안전성 평가를 위한 작물 성분 DB 구축)

  • Kim, Eun-Ha;Lee, Seong-Kon;Park, Soo-Yun;Lee, Sang-Gu;Oh, Seon-Woo
    • Journal of Plant Biotechnology
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    • v.45 no.4
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    • pp.289-298
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    • 2018
  • The Biosafety Division of the National Academy of Agricultural Science has developed a 'Crop Composition DB' that provides analytical data on commercialized crops. It can be used as a reference in the 'Comparative Evaluation by Compositional Analysis' for the safety assessment of genetically modified (GM) crops. This database provides the composition of crops cultivated in Korea, and thus upgrades the data to check the extent of changes in the compositional content depending on the cultivated area, varieties and year. The database is a compilation of data on the antioxidant, nutrient and secondary metabolite compositions of rice and capsicum grown in two or more cultivation areas for a period of more than two years. Data analysis was conducted under the guidelines of the Association of Official Analytical Chemists or methods previously reported on papers. The data was provided as average, minimum and maximum values to assess whether the statistical differences between the GM crops and comparative non-GM crops fall within the biological differences or tolerances of the existing commercial crops. The Crop Composition DB is an open-access source and is easy to access based on the query selected by the user. Moreover, functional ingredients of colored crops, such as potatoes, sweet potatoes and cauliflowers, were provided so that food information can be used and utilized by general consumers. This paper introduces the feature and usage of 'Crop Composition DB', which is a valuable tool for characterizing the composition of conventional crops.

The Roles of Dietary Polyphenols in Brain Neuromodulation (뇌 신경조절에서의 식이 폴리페놀 화합물의 역할)

  • Lee, Hyeyoung;Lee, Heeseob
    • Journal of Life Science
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    • v.28 no.11
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    • pp.1386-1395
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    • 2018
  • Over recent years, it has become evident that the central nervous system bidirectionally interacts with the gastrointestinal tract along the gut-brain axis. A series of preclinical studies indicate that the gut microbiota can modulate central nervous system function through a multitude of physiological functions. Polyphenols are ubiquitous plant chemicals included in foods such as fruits, vegetables, tea, coffee and wine, and their consumption is directly responsible for beneficial health effects due to antioxidant, anti-inflammatory, antimicrobial, immunomodulatory, anticancer, vasodilating, and prebiotic-like effects. There is increasing evidence that dietary polyphenol can contribute to beneficial effects in neuronal protection acting against oxidative stress and inflammatory injury as well as in cognitive functions. In this paper, we overview the neuroprotective role of dietary polyphenols especially focusing on the neuroinflammation and neurovascular function by interaction with the gut microbiome. Polyphenol metabolites could directly act as neurotransmitters crossing the blood-brain barrier and modulating the cerebrovascular system or indirectly modulating gut microbiota. In addition, evidence suggests that dietary polyphenols are effective in preventing and managing neurological disorders, such as age-related cognitive decline and neurodegeneration, through a multitude of physiological functions. Dietary polyphenols are increasingly envisaged as a potential nutraceuticals in the prevention and treatment of neurological disorders, because they possess the ability to reduce neuroinflammation, to improve memory and cognitive function and to modulate the gut microbiota.

Protective effects of Sasa quelpaertensis Leaf Residue Extract against Potassium Oxonate-induced Hyperuricemia in Mice (생쥐에서 제주조릿대 잎 잔사 추출물의 고요산 혈증 저감 효과)

  • Jang, Mi Gyeong;Song, Hana;Lee, Ju Yeop;Ko, Hee Chul;Hur, Sung-Pyo;Kim, Se Jae
    • Journal of Life Science
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    • v.29 no.1
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    • pp.37-44
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    • 2019
  • Leaves of Sasa quelpaertensis Nakai are used in folk medicine for their anti-inflammatory, antipyretic, and diuretic properties. To ensure efficient utilization of S. quelpaertensis leaf, we previously reported a preparation method for phytochemical-rich extract (PRE) using the leaf residue, which was produced after hot water extraction. This study was undertaken to evaluate the hypouricemic potential of S. quelpaertensis leaf PRE in potassium oxonate (PO)-induced hyperuricemic mice. The administration of PRE significantly reduced serum uric acid (UA), blood urea nitrogen (BUN), and serum creatinine levels and increased urine UA and creatinine levels in the PO-induced hyperuricemic mice. It also reduced liver UA levels and xanthine oxidase (XA) activity. A histological analysis revealed that PRE administration protected against PO-induced liver damage, pointing to anti-inflammatory and cytoprotective effects in PO-induced hyperuricemic mice. We analyzed the transcriptome response to PRE administration in PO-induced hyperuricemic mice using RNA sequencing (RNA-Seq) in kidney tissues. The administration of PRE mainly enriched genes involved in mediating immune and inflammatory responses and the metabolic pathway. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the metabolic pathway, purine metabolism, and antibody biosynthesis were the major pathways altered in the PRE and PO groups. These results suggest a potential role for PRE in the prevention and treatment of hyperuricemia with inflammation.

The Periodical Formation and Phase of Change of Cheongpyeongsa Temple Zen Garden (청평사(淸平寺) 선원(禪園)의 시대적(時代的) 형성(形成)과 변천상(變遷相))

  • Yoon, Young Hwal
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.29 no.1
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    • pp.1-13
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    • 2011
  • Cheongpyeongsa Temple was originally built in the early years of Goryeo Dynasty, but its current structural framework was made by the Lord Jinrakgong Lee Ja Hyeon(1061-1125) of the middle Goryeo period based on the Zen thought after he began living in the Cheongpyeong mountain around the temple in 1089. The purpose of this study is to conceptualize, based on old documents, historical changes of the appearance and survival of man-made structures with in the Zen garden formed and developed after Lee Ja Hyeon laid the foundation for Munsuwon Zen garden. Among the eight, outside-the-temple hermitages built at the time of Lee Ja Hyeon's Munsuwon Zen garden, only three hermitages, which are Sik-am, Gyeonseong-am, Yangshin-am had been remaining thanks to restoration and repair until late Joseon Dynasty and preserved as symbolic hermitages. Also, the Yeongji Pond built at the time of Lee Ja Hyeon still remains as precious landscape relics which is meaningful as a genuine Goryeo-period pond. The nine pine trees said to be planted by Lee Ja Hyeon remained until middle 1800s through their descendant trees. When the Buddhist monk Bowoo Daesa(1509-1565)changed the name to Cheongpyeongsa Temple in middle Joseon based on the Munsuwon Zen garden built by Lee Ja Hyeon and greatly expanded it, he newly built and expanded all buildings inside the temple except for Neunginjeon(main temple building), resulting in the present temple structure. In addition, by greatly enhancing the level of scenery by reconstructing Yeongji Pond outside the temple area and transplanting garden plants from the royal court, he made Cheongpyeongsa Temple the most prosperous Zen garden in its history. But after the mid-1800s, which is late Joseon period, Cheongpyeongsa Temple failed to thrive further and began to decline, and so currently most buildings of the Zen garden have disappeared except for some parts of the temple and other facilities are neglected.

Recent Advances in the Biotechnological Production of Natural Vanillin (생물공학에 기반한 천연 바닐린 생산에 관한 최근 연구)

  • Kim, Hyun-Song;Kim, Young-Ok;Lee, Jin-Ho
    • Journal of Life Science
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    • v.31 no.11
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    • pp.1046-1055
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    • 2021
  • Vanillin is the primary flavor and fragrance compound of natural vanilla and is extensively used in the food, beverage, perfumery, pharmaceutical industries, and other applications. Vanillin can be produced by chemical synthesis, extraction from vanilla plants, microbial bioconversion of natural precursors to vanillin, and direct fermentation using glucose. Currently, most commercially available vanillin is produced by extraction from cured vanilla pods and by chemical synthesis using guaiacol and glyoxylic acid as starting raw materials. Due to environmental issues, health complaints, preference for natural sources, and the limited supply and soaring price of natural vanilla, biotechnology-based vanillin production is regarded as a promising alternative. As many microorganisms that are able to metabolize several natural precursors, including ferulic acid, eugenol, isoeugenol, and lignin, and accumulate vanillin, have been screened and evaluated, myriad strategies and efforts have been employed for the development of commercially viable production technology. This review outlines the recent advances in the biotechnological production of natural vanillin with the use of these natural precursors. Moreover, it highlights the recent engineering approaches for the production of natural vanillin from renewable carbon sources based on the de novo biosynthetic pathway of vanillin from glucose, together with appropriate solution strategies to overcome the challenges posed to increase production titers.

Effects of Ferulic Acid-Based Preparation on Performance, Carcass Characteristics, Blood Profiles, and Intestinal Microflora of Broiler Chicks (페룰산의 사료 내 첨가가 육계 생산성, 도체 특성, 혈중 성분 및 장내 균총에 미치는 영향)

  • Kim, Yong-Ran;Lee, Sang-Woo;Kim, Eun-Jip
    • Korean Journal of Poultry Science
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    • v.49 no.1
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    • pp.45-51
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    • 2022
  • This study aimed to evaluate the dietary effects of ferulic acid-based preparation on the performance, blood profiles, and microflora of broiler chicks. A total of 400 chicks (1 day old Ross × Ross) were divided into 20 groups and assigned to four treatments: a basal diet (control) or a treatment diet containing 0.1%, 0.2%, or 0.3% ferulic acid-based preparation. The birds were fed from 1 to 30 d of age. Average daily gain improved compared to that in the control group in all periods (P<0.05). There was no significant difference between treatments in Average daily feed intake during all periods. Feed conversion ratio during starter (day 1 to 21) and total day 1 to 30 periods significantly improved (P<0.05) in the treatments containing ferulic acid-based preparation compared to that in the control. The total cholesterol levels in all the treatments containing ferulic acid-based preparation were significantly lower than that in the control (P<0.05). The globulin content was significantly higher (P<0.05) in the treatment with 0.2% and 0.3% ferulic acid-based preparations than that in the control and 0.1% ferulic acid-based preparation treatments. Among the treatments, there was no significant difference in the total number of cecal bacteria. The number of lactic acid bacteria significantly increased in all treatments containing ferulic acid-based preparation compared to that in the control (P<0.05). The number of coliforms significantly decreased in all treatments containing ferulic acid-based preparation compared to that in the control (P<0.05). In conclusion, the addition of ferulic acid-based preparation to the diet improved broiler performance, blood profiles, and intestinal flora.