• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.317-326
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• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its productivity and quality. Two cultivars of B. falcatum, originated from Jeongseon, Korea and Mishima, Japan were used. Some of the results obtained are as follows : Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of saikosaponin, but low saikosaponin content than that of Mishima. Both cultivars seeded on March 20 had long main root, big stem diameter, few stem branch, and high saikosaponin c content compared to those of late seeded one, April 30. Growth characteristics such as plant height, stem diameter, stem branch number, shoot weight, root diameter, root fresh and dry weight, and root branch number were increased in a low planting $density(30\;{\times}\;15cm)$, but the content of saikosaponin was not affected by planting density. Jeongseon and Mishima cultivars seeded on April 10 with $30\;{\times}\;15cm$ planting density and April 30 with $30\;{\times}\;10cm$ planting density contained the highest total saikosaponin levels, respectively. However, average root dry weight were not affected by planting time or density in both Bupleurum cultivars.

• Korean Journal of Poultry Science
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• v.33 no.1
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• pp.49-55
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• 2006

An experiment was conducted to investigate the effects of feeding Bupleurum falcatum L.(BFL) on performance, blood component and hormonal secretion in broiler breeder hens. A total of 800 broiler breeder hens were allotted to four treatments(BFL ; 0, 0.5, 1.0, 2.0%) with four replications for eight weeks. Weight gain was not significantly different between the groups in growing period($20{\sim}24$ weeks), but egg production and egg mass of broiler breeder hens fed BFL were significantly(p<0.05) higher than those of the control groups in laying period($24{\sim}28$ weeks). Dietary BFL did not affect serum triglyceride concentration, whereas serum cholesterol concentration was significantly increased(p<0.05) in 2.0% BFL-fed groups. No statistical differences were found in serum glucose, Ca, P, Mg, total protein, albumin, GGT, AST, ALT and BUN concentrations. However, albumin/globulin ratio was statistically higher in BFL-fed groups compared to the control groups(p<0.05). Serum estrogen concentrations were significantly increased by feeding a diet containing 0.5% BFL, but progesterone were not influenced. Serum IGF-I of BFL groups was greater than that of control groups at 28 weeks(p<0.05). In conclusion, the results suggest that the dietary addition of BFL into broiler breeder diets improved egg production, serum estrogen and IGF-I concentration, and decreased serum cholesterol concentration and albumin/globulin ratio.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.234-242
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• 2000

There are two cultivars, 'Jeongsun' and 'Mishima' of Bupleurum falcatum L., a medicinal plant, have been cultivated in Korea. Two cultivars were evaluated and compared in details for major growth characters and their seasonal changes. Jeongsun was higher in stem length and root weight per plant, and earlier in maturity than Mishima, while Mishima was higher in top weight per plant and the ratio of outer phloem layer(OPL) to whole root, and more in branch number. Coefficients of variations of most growth characters were higher in Mishima than in Jeongsun. In relationship between root and shoot growth characters, root weight, root diameter, etc. were positively correlated with stem thickness, top weight, branch number, etc. in both cultivars. In comparison of seasonal changes for growth characters between two cultivars, stem length and node number were almost not increased after September, but root length and branch number tended to increase until November. There were continuous increases of top and root weights in both cultivars after September. Increase rates of top and root weights were higher in Mishima and Jeongsun, respectively. The weight ratio of root part to whole plant in Jeongsun and the ratio of OPL to whole root in Mishima were higher during whole growth stages; especially Mishima showed their continuous increases after October. Jeongsun flowered earlier (August 17) than Mishima (September 16) by one month. Complete flowering date was on August 30 in Jeongsun and on September 30 in Mishima. Basic difference in plant growth between two cultivars was maturity, so shoot growth almost stopped after October in Jeongsun, and continued vegetative growth until November in Mishima with late flowering habit. These results indicate that higher root weight in Jeongsun is due to its earlier flowering character leading to relatively longer time for root growth, as compared with Mishima.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.344-352
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• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its quality. Furthermore, the effect of cultural methods and plant growth regulators$(GA_3,\;IAA,\;Kinetin)$ on the quality of B. falcatum were also investigated. In this study, Jeongseon cultivar collected in Korea and Mishima cultivar introduced from Japan were used. Some of the results obtained are as follows. Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of SSa and TSS, but low SSc content than that of Mishima. Although fresh and dried root weight of both cultivars were not affected by plant growth regulators treatment. compared with the untreated one, increasement of TSS content were made by $GA_3$ 100 ppm, IAA 50 ppm or 10 ppm and kinetin 10 ppm treatment in Jeongseon cultivar, and by $GA_3$ 10 ppm and IAA 100 ppm or 10ppm treatment in Mishima cultivar applied on June 1.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.353-365
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• 2002

This study was conducted to investigate the effect of cultural methods(cutting, flower removal) on growth and quality of B. falcatum L. Jeongseon local cultivar collected in Korea and Mishima local cultivar introduced from Japan were used. Some of the results obtained are as follows; Jeongseon local cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon local cultivar showed tall plant height, high root fresh and dry weight, and high levels of saikosaponin-a and total-saikosaponin, but low saikosaponin-c content than that of Mishima. Both cultivars seeded on March 20 had longer main root, bigger stem diameter, few stem branch, and high saikosaponin-c content compared with those of late seeded one, April 30. Growth characteristics such as plant height, stem diameter, stem branch number, shoot weight, root diameter, root fresh and dry weight, and root branch number were increased in a low planting $density(30{\times}15\;cm)$, but the content of saikosaponin was not affected by planting density. Jeongseon and Mishima cultivars seeded on April 10 with $30{\times}15\;cm$ planting density and April 30 with $30{\times}10\;cm$ planting density contained highest total saikosaponin levels, respectively. However, average root dry weight was not affected by planting time or density in both Bupleurum cultivars. Both cutting and flower removal increased the length of main root, fresh and root weight, and saikosaponin-a, saikosaponin-d and total-saikosaponin in Jeongseon cultivar than that of Mishima. As the harvesting time was delayed, plant height, stem diameter, shoot weight, length of main root, root fresh and dry weight were increased, but content of saikosaponin-a, saikosaponin-d and total-saikosaponin were decreased.

• Korean Journal of Environment and Ecology
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• v.22 no.1
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• pp.18-34
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• 2008

Based on external morphology, each of five species can be classified into three groups: 1) B. falcatum group (B. falcatum, B. scorzonerifolium), 2) B. euphorbioides group (B. euphorbioides) and 3) B. longiradiatum group (B. longiradiatum, B. latissimum). B. falcatum group has cauline leaves linear or lanceolate in shape and attenuate at base and not surrounding the stem. In contrast, B. longiradiatum group and B. euphorbioides group have cauline leaves ovate, lanceolate or panduriform in shape and auriculate or cordate at base and completely surrounding the stem. The inflorescence is basically compound umbels terminated at the apex of stem. But B. euphorbioides group is small in size and pedicels are rather short and the number of the pedicel is ca. 20. On the other hand, B. longiradiatum and B. falcatum groups are large in size and their pedicels are long and the number of the pedicel is around 10. The pore of pollen aperture of B. longiradiatum and B. latissimum is partially projected or not while those of B. falcatum group and B. euphorbioides is usually remarkably projected. The number of somatic chromosomes was counted as 2n=20 in B. falcatum, 2n=12 in B. scorzonerifolium and B. longiradiatum, and 2n=16 in B. euphorbioides and B. latissimum. Although chromosome numbers of B. euphorbioides and B. latissimum are the same, the two species are not likely to relate because the karyotypes of the two species are different from each other. Based on these observations, it can be concluded that B. latissimum is most closely related to B. longiradiatum. However, molecular data indicated that the species is probably related to B. bicaule distributed in central Siberia. In terms of conservation of B. latissimum, overexploitation by human and grazing by goat are most threatened factors.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.125-125
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• 1993

Saikosaponin은 한방에서 중요하게 사용되고 있는 시호의 항염증작용 성분으로서 주로 염증의 후기 단계에 작용하고 있다. 그러나 현재 시호는 생약자체로만 사용되고 성분이 약물로서 개발되지 못하고 있다. 그것은 Saikosaponin의 용해성이 극히 저조하여 제제화하기 힘든 때문이다. 연자는 시호 Saponin의 용해성을 증가시킬 목적으로 시호 Saponin의 하나인 saikosaponin-a를 Pyridine 중에서 succinic anhydride와 반응시켜 6"-hemisuccinyl saikosaponin-a를 얻고 이를 THF에 용해한 후 KOH와 반응시킨 후 다량의 THF를 추가하여 hemisuccinyl esler의 potassium염을 합성하였다. 이 화합물의 용해도를 원래의 Saponin과 비교시험하였다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.384-391
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• 1997

In the cultivation of Bupleurum falcatum, one of the problems to surmount is long-term germination period and unstable germination. This experiment was done to examine the effect of $GA_3$ concentration [0(water), 0.01, 0.lmM], chilling, their treatment period [2, 4, 8 days ($GA_3$) : 0, 2, 4 weeks (chilling)] and light quality (red, white, dark) given during the period as pretreatment before sowing on the seed germination of its two cultivars (cv. Jaerae, cv. Jangsu). Light treatment was given during all the periods of $GA_3$ treatment or for 0, 2, 4 days at the end of the chilling treatment. There was no difference in the mean germination rate between the levels of all the treatments except the $GA_3$ concentration meaning that water imbibition and $GA_3$ treatment had the same effect. As light quality treatment during the water imbibition was forced, the mean germination rate of Jaerae, 2 to 4 days imbibition period or red light was more increased or accelerated compared to the other levels of the same treatment, respectively. The rate of Jaerae not affected by the light quality was the greatest in the 2 days water imbibition while the rate of Jangsu was the greatest when water-imbibed for 4 days or treated by red light. No chilling before sowing showed the highest rate due to the light quality and white light forced after sowing had greater rate than the dark treatment. Although there was no difference between the rates of light quality treatment levels in the condition of no chilling before sowing and white light treatment after sowing, the rate of Jangsu was enhanced or accelerated only under illumination during 2 days water imbibition before sowing.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.183-187
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• 2015

The electron donating ability, elastase inhibitory, procollagen synthesis and Matrix metalloprotease-1 (MMP-1) activities were measured in order to verify the anti-wrinkle properties of extracts from Buplerum falcatum as a functional ingredient for cosmetic products. Electron donating ability and elastase inhibition activities were 80 and 52% at a dose of $1,000{\mu}g/mL$ of B. falcatum 70% ethanol extract. Pro-collagen synthesis was increased with the increase concentration of B. falcatum extract on CCD-986sk in addition to decrease the amount of protein of MMP-1. The results suggested that B. falcatum extract can be used to reduced electron donating ability, elastase, pro-collagen synthesis and MMP-1 activity and is a potential candidate for cosmedical materials.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.61-65
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• 1995

Karyotype analysis was carried out in four lines of Bupleurum falcatum L. cultivated in Korea and SDS-PAGE was applied to determine the seed protein profiles among the lines. Chromosomes were classified into two groups, large and small ones. Two kinds of karyotype, 2n=20 and 2n=26, were identified. Chromosome 1 of 2n=20 were all submedian, while that of 2n=26 were median. Chromosomes 2, 3 and 5 of 2n=20 showed polymorphism in size and arm-ratio. Chromosome 2 was submedian, while others were median in the line of 2n=26. Karyotypcs of cultivars native of Korea were similiar each other, while those introduced from Japan showed different patterns. In SDS PAGE gels, qualitative difference s in high molecular weight proteins, more than 45KD, were detected among the lines. The numbers of specific band were three in lines of 2n=20 and two in 2n=26.