• Title/Summary/Keyword: 시험종

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Domestic Test Species for Aquatic Toxicity Assessment in Korea (수생태계 독성평가에 적용 가능한 국내 시험종 선정)

  • An, Youn-Joo;Nam, Sun-Hwa;Lee, Jae-Kwan
    • Korean Journal of Ecology and Environment
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    • v.40 no.1
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    • pp.1-13
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    • 2007
  • The use of aquatic species in ecotoxicity research is well established in developed countries. However, there are limitations of using the species that are not native to Korea, and the toxicity data produced by domestic test species are significantly needed to reflect the domestic situation. The purpose of this study was to investigate the domestic species that can be applicable for the aquatic toxicity assessment in Korea. Aquatic toxicity data were collected in the framework of the project 'Development of integrated methodology for evaluation of water environment' to obtain a range of test species used for aquatic toxicity assessment internationally. The test species collected were evaluated in terms of domestic distribution based on the reliable references and the advices of experts. We figured out the 71 test species native to Korea. They included 7 fish, 26 invertebrates (2 annelids, 2 bryozoa, 13 crustaceans, 3 insects, 4 mollusc, 1 platyhelminth, and 1 protozoan), 26 plants (9 diatoms, 14 green algae, 3 macrophytes), and 12 others (2 amphibians, 3 bacteria, 6 blue-green algae, and 1 fungus). The result of this study should be a very useful information for ecotoxicity assessment in aquatic ecosystem, especially in choosing the test species applicable for the ecotoxicity in Korea hereafter.

Fundamentals of Ecotoxicity Evaluation Methods Using Domestic Aquatic Organisms in Korea : (II) Water Flea (국내 생물종을 이용한 생태독성평가 기반연구 : (II) 물벼룩류)

  • An, Youn-Joo;Nam, Sun-Hwa;Lee, Woo-Mi
    • Korean Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.357-369
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    • 2007
  • Water fleas are very important organisms in aquatic ecosystem because they are key constituents of food webs as either glazers or foods for predators. Daphnia magna is a representative test species and it has been extensively used in ecotoxicity evaluation. However, Daphnia magna has not been found yet in Korean water environment. There are limitations of using the foreign species such as Daphnia magna to reflect domestic situations in Korea. Consequently it is mandatory to find domestic species suitable for bioassay, and to develop corresponding toxicity test methods using the domestic species. In this study, we widely collected the domestic and foreign toxicity test methods performed by using domestic water fleas in Korea. The 58 test methods were collected from the standard methods (OECD, US EPA, ASTM), government reports, SCI papers and domestic papers. Ten domestic water fleas selected were Bosmina longirostris, Ceriodaphnia dubia, Ceriodaphnia reticulata, Daphnia obtusa, Daphnia puex, Moina macrocopa, Moina micrura, Simocephalus mixtus, Simocephalus serrulatus, and Simocephalus vetulus. We suggested the domestic ecotoxicity test methods using domestic water fleas in Korea through providing a range of test conditions, and future directions for toxicity test using water flea. This study could be a useful basis for establishing the aquatic toxicity test methods with domestic organisms in Korea.

Fundamentals of Ecotoxicity Evaluation Methods Using Domestic Aquatic Organisms in Korea: (KII) Green Algae (국내 생물종을 이용한 생태독성평가 기반연구: (III) 녹조류)

  • An, Youn-Joo;Nam, Sun-Hwa;Baek, Yong-Wook
    • Korean Journal of Ecology and Environment
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    • v.41 no.2
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    • pp.117-127
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    • 2008
  • Green algae are important primary producers in aquatic ecosystem, and they are sensitive test species in bioassay. Green algae are broadly used to assess the adverse effects of various chemicals by measuring the inhibition of metabolism, reproduction and survival. In this study, we extensively gathered domestic and foreign toxicity test methods conducted using green algae, which are distributed in Korean water environment. Selected eight domestic green algae were Chlamydomonas reinhardtii, Desmodesmus subspicatus (=Scenedesmus subspicatus), Scenedesmus abundans, Scenedesmus acutus, Scenedesmus quadricauda, Podohedriella falcata (=Ankistrodesmus falcatus), Pseudokirchneriella subcapitata (=Selenastrum capricornutum), and Chlorella vulgaris. Forty four test methods were collected from the standard test ones, government reports, SCI papers and Korean research papers. P. subcapitata and D. subspicatus are the most common test species recommended by the standard test methods. Initial cell density and dilution water were the main differences among the test methods we collected. We proposed the suitable ecotoxicity test methods based on domestic green algae in Korea. This study could be a fundamental basis to establish the ecotoxicity test methods by green algae distributed in Korea.

Fundamentals of Ecotoxicity Evaluation Methods using Domestic Aquatic Organisms in Korea : (I) Fish (국내 생물종을 이용한 생태독성평가 기반연구 : (I) 어류)

  • Nam, Sun-Hwa;Yang, Chang-Yong;An, Youn-Joo;Lee, Jae-Kwan
    • Korean Journal of Ecology and Environment
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    • v.40 no.2
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    • pp.173-183
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    • 2007
  • The Ministry of Environment plans to introduce the Whole Effluent Toxicity (WET) system in Korea. The WET test is well established in developed countries with recognizing of the limitations of physicochemical analysis method and potential risk of chemicals in water medium. Therefore, it is essential to build the ecotoxicity infrastructure for the induction of WET test. In this study, we extensively collected the domestic and foreign toxicity test methods which employ native test species to Korea. And we suggested that the domestic ecotoxicity test methods with domestic test species in Korea through extracting the range of test conditions. Five domestic fish species selected were Carassiu auratus (Crucian carp), Cyprinus carpio(Common carp), Gasterosteus aculeatus (Three spine stickleback), Misgurnus anguillicaudatus (Oriental weather fish) and Oryzias latipes (Japanese medaka), The toxicity test methods with native test species to Korea were collected from the standard methods (OECD, U.S. EPA, ASTM), government reports, SCI papers and domestic papers. We collected the 32 test methods, and suggested the suitable aquatic toxicity test methods for fish. It is expected that this study could prove a useful information to establish the ecotoxicity test methods with domestic aquatic organisms in Korea henceforth.

Longitudinal Bonding Strength Performance Evaluation of Larch Lumber (낙엽송 소경각재의 종접합 성능평가)

  • Lee, In-Hwan;Pack, Ju-hyun;Song, Da-bin;Hong, Soon-Il
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.1
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    • pp.85-92
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    • 2018
  • In order to use glued built up timber beam as a structural member for post and beam construction, it must be possible to manufacture long-span lumber. In this study, the researchers conducted a performance evaluation for longitudinal bonding of lumber (cross-section $89{\times}120mm$) made from larch. The specimens were prepared in six different forms using the longitudinal bonding method. The bonding strength of these specimens was tested through tensile strength tests and bending strength tests. The tensile strength test result of the longitudinally bonded parts was better than that of the double lap specimens. And, the tensile strength value of the scarf specimen was better than that of the hooked scarf specimen. The tensile strength of the GFRP (Glass Fiber Reinforced Plastic) rod insertion bonding specimen was 3.6 MPa, which was the highest. As for the bending strength test result of the longitudinally bonded part, the average MOR (modulus of rupture) of the specimen where a GFRP rod was inserted and bonded measured 29 MPa, while the specimens of other bonded parts showed a MOR no more than 11 MPa. Toughness destruction was observed in specimens where a GFRP rod was insertion-bonded. The rest of the specimens showed brittle destruction. The average MOR strength of the Rod + Lap specimen was 30.5 MPa, which was the highest among all longitudinally bonded specimens. The bending strength of the Rod + Lap specimens showed an effective strength that was 66% of that of the control group which were not longitudinally bonded.

Development of Sediment Toxicity Test Protocols using Korean Indigenous Marine Benthic Amphipods (국내산 저서 단각류를 이용한 퇴적물 독성시험법 개발에 관한 연구)

  • Lee, Jung-Suk;Lee, Seung-Min;Park, Gyung-Soo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.147-155
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    • 2008
  • A series of experiments were conducted to find standard test organisms and to develop test protocols for sediment toxicity tests using indigenous amphipods inhabited in Korean coastal environments. The indigenous amphipods Mandibulophoxus mai and Monocorophium acherusicum were well associated with various sediment substrates from sand to mud. The tolerance limits to various physico-chemical factors affecting bioassay results such as temperature, salinity and total ammonium as well as the sensitivities to contaminants in water and sediments were investigated using M. mai and M. acherusicum in the present study. These amphipods were tolerable to the adequate ranges of salinity ($10{\sim}30\;psu$), temperature ($10{\sim}25^{\circ}C$) and ammonia (<50 ppm). They have relevant sensitivities to the reference toxicants, dissolved cadmium as well as other metals and organic pollutants, when compared to the standard test species used in other countries. Field-sediment toxicity tests revealed that M. mai would be more sensitive to sediment-associated pollutants than M. acherusicum, while the sensitivity of M. acherusicum was comparable to those of other sediment test species in other countries. Overall results of this first attempt to develop an amphipod sediment toxicity test protocol in Korea indicated that M. mai and M. acherusicum should be applicable in the toxicity assessment of contaminated sediments, following the further evaluation encompassing various ecological and toxicological evaluation and the standardization of test method.

Selection of Domestic Test Species Suitable for Korean Soil Ecological Risk Assessment (토양생태 위해성평가를 위한 국내 서식 토양독성 시험종 선별 연구)

  • Kim, Shin Woong;Kwak, Jin Il;Yoon, Jin-Yul;Jeong, Seung-Woo;An, Youn-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.5
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    • pp.359-366
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    • 2014
  • For an efficient and reasonable management scheme for protecting the soil environment, a soil ecological risk assessment (ERA) method should be developed prior to utilization, based on the contemporary uses and situations of each country. The Korean environmental policy focusing on soil protection is currently accelerating the development of the soil ecological risk assessment method. The soil ERA requires toxicological data on various trophic levels in the soil environment, and ultimately uses PNEC (Predicted No Effect Concentration), which is derived from collected toxicological data. Therefore, test species that are used to generate toxicity data are essential for conducting reliable ERA. This study aimed to select domestic test species for potential use in a reliable Korean ERA. Copper (Cu) and Nickel (Ni) were identified as target substances, with toxicity data (Cu, Ni) and standard test methods being collected to determine candidate species. The candidate species were first classified by soil trophic level, and then sorted into final domestic species. Forty out of 166 domestic species were determined as potential standard test species, whereas 17 out of 120 species were determined as potential Cu and Ni test species. Finally, this study presented potential soil test species based on the characteristics of the domestic soil environment, and established a preliminary step toward developing a reliable Korean soil ERA method.

Development of Mechanical Test Techniques for Irradiated Zircaloy Cladding in Hot Cell (조사 지르칼로이 피복관의 기계적 특성시험 기술 개발)

  • 김도식;홍권표;주용선;안상복;송웅섭;유병옥;김기하
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.213-213
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    • 2003
  • 고온 및 고압의 가혹한 방사선 분위기에서 사용되는 핵연료 피복관은 중성자 조사 및 수소화합물의 생성 등으로 인하여 기계적 성질이 저하된다. 따라서 조사된 핵연료 피복관의 손상기준 확립과 안전성 해석을 위해서는 연성 및 강도 등 기계적 특성을 정확히 이해하여야 할 필요가 있다. 핵연료 피복관의 종 및 횡 방향 인장특성 평가를 위하여 개발된 기존의 다양한 시험법들을 비교하고, 핫셀시험에 적합한 인장시험법을 개발하였다. 피복관의 종방향 인장시편은 튜브시편 또는 게이지부 내에서 균일한 변형률 분포를 얻도록 설계된 도그본 튜브시편(그림 1)을 사용한다. 피복관의 횡방향 인장시험에 사용되는 링시편(그림 2)은 게이지부 내에서 균일한 단축 원환변형율 분포 또는 평면변형율 조건을 나타내도록 설계한다. 연소 또는 조사된 피복관으로부터 시편을 제작하기 위해서는 핫셀 내에서 작업 이 가능한 방전가공기(그림 3)를 사용한다. 피복관의 종방향 인장시험용그립(grip)은 핀-부하형이며, 횡방향 인장시험의 경우는 시험 동안 시편의 곡률이 일정하게 유지 되도록 그립의 형상 및 치수를 결정한다(그림 4). 피복관의 종 및 횡방향 강도와 변형 등 기계적 특성을 평가하기 위한 응력-변형율 곡선은 시험기의 복합 강성(K)을 고려하여 결정한다. 이상과 같이 검토된 인장시험법은 피복관의 안전성 해석(safety analysis)과 관련 규정(regulatory)에서 사용되는 피복관 손상기준(fuel damage criteria)의 개선에 필수적인 자료를 제공한다.

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Characteristics and Toxicity Sensitivity of Korean Dominant Species Daphnia galeata for Ecotoxicity Testing: Comparative Study with Daphnia magna (생태독성실험을 위한 한국 우점종 유리물벼룩(Daphnia galeata)의 특성 및 독성민감도: 큰물벼룩(Daphnia magna)과의 비교연구)

  • Cui, Rongxue;Kwak, Jin Il;An, Youn-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.4
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    • pp.193-200
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    • 2016
  • Water flea is a widely used test species in the aquatic ecotoxicity test. In Korea, D. magna is currently used as a standard test species, however that do not inhabit in the Korean aquatic ecosystem. In this study, Daphnia galeata, which is a dominant species in the Korean aquatic ecosystem, was collected from domestic lake and investigated to suggest the D. galeata for ecotoxicity assessment in Korea. We investigated the characteristics, life span, and toxicity sensitivity compared with D. magna. The life span test of D. galeata was performed in this study, and then the results were compared to the sensitivity with D. magna to confirm the applicability for ecotoxicity assessment. The 48h-L(E)C50 values for seven heavy metals (As, Cd, Cr, Cu, Hg, Ni, and Zn) of D. galeata and D. magna were collected and analyzed. As a results, shorter lifetime, less reproduction, smaller body size of D. galeata were observed compared with D. magna. D. galeata was similar or more sensitive than D. magna for seven heavy metals. Therefore, we propose that D. galeata is a suitable test species for ecotoxicity testing in Korea.