• Title/Summary/Keyword: 시료채취기준

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Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines (신선편이 농식품 생산라인의 환경미생물 오염도 측정을 위한 ATP 검사법의 이용)

  • Choi, Ji-Weon;Lee, Hye-Eun;Kim, Chang-Kug;Kim, Won-Bae;Kim, Ji-Kang
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.62-66
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    • 2012
  • With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

Analysis of Essential Oils from the Peel of Mandarine (Citrus unshiu Marc. Var. Okitsu) (온주 밀감의 껍질로부터 정유의 분리 및 분석)

  • Kim, Yu-Kyoung;Hyun, Seung-Won;Ko, Young-Hwan
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1178-1183
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    • 1999
  • Essential oils were isolated from the peel of mandarin (Citrus unshiu Marc. Var. Okitsu) cultivated in Cheju Island. Among three different isolation methods investigated, simultaneous distillation gave higher yield 1.14%(w/w) than solvent extraction or cold pressing. The densities of essential oils were between 0.8409 and 0.8530, which showed no significant effect of the isolation methods. Some variations in the number of constituents detected and the content of d-limonene in the peel oils were observed depending upon the isolation methods. The citrus fruit was collected seasonally from the field and used as a sample for analysis. The peel thickness and the peel content of citrus fruit were around 2 mm and about 17% on wet weight basis, respectively, in harvest season. The maximum yield of essential oils was obtained in September from citrus peels just prior to ripeness and thereafter the yield had a tendency of decrease but remained over 1%(w/w). The relative content of d-limonene, the major constituent of citrus peel oils. increased gradually with ripening up to 68.69%(relative peak area, RPA). The other minor constituents were ${\gamma}-terpinene,\;{\beta}-elemene$, farnesene, hexadecanoic acid, ${\alpha}-pinene,\; {\beta}-myrcene$ and linalool in the decreasing order of their own contents of 7.75, 2.96, 2.29, 1.76, 1.63, 1.56 and 1.46%(RPA).

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Comparative Study on the Adsorptive Loss of Reduced Sulfur Compounds (RSC) by the Selection of Tubing Materials (튜빙의 종류에 따른 환원황화합물들의 흡착손실 비교 연구)

  • Kim Ki-Hyun;Ahn Ji-Won;Choi Ye-Jin
    • Journal of the Korean earth science society
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    • v.26 no.7
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    • pp.668-673
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    • 2005
  • To collect or transfer samples of gaseous pollutants, various types of tubing are used. Hence, to analyze the uncertainties associated with the use of tubings, a series of comparative test were designed and conducted using the RSC standards with different concentration ranges. For the purpose of this study, we prepared tubings made of six different types of material which include: [1] silco-steel (S1), [2] stainless steel (S2), [3] silicone (S3), [4] PTFE Teflon (T1), [5] tygon (T2), and [6] brass (B). The patterns of RSC loss on to tubing walls, when compared on the basis of the least reactive material S1, exhibited that the extent of RSC loss varied dynamically. It was found that Teflon is highly stable. However, other materials tend to exhibit contrasting patterns of loss. S2 and B show significant loss of light RSC $(H_2S\;and\;CH_3SH)$, while S3 and T2 experience notable loss of heavy RSC (DMS and DMDS).

Estimation of Denitrification in the Ganghwa Tidal Flat by a Pore Water Model (공극수 모델로 추정한 강화도 갯벌의 탈질산화 작용)

  • Na, Tae-Hee;Lee, Tong-Sup
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.10 no.1
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    • pp.56-68
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    • 2005
  • We measured nitrate and other nutrients in sediment pore waters retrieved from three sites at the southern upper-tidal flats of the Ganghwa Island. Denitrification rate is estimated by applying a simple 1-D model to the nitrate profiles. Results from Jangwha and Dongmak sites are $7.8{\sim}9.4{\times}10^{-7}{\mu}mol{\cdot}cm^{-2}{\cdot}sec^{-1}$, and $1.4{\sim}3.6{\times}10^{-7}{\mu}mol{\cdot}cm^{-2}{\cdot}sec^{-1}$, respectively. Rates are comparable to those reported around the world in an order of magnitude. Denitrification was lower in summer. The rates were about 1.5 times higher at site where the surface sediments consist of relatively coarser particles. This implies that particle size would control the reactant supply to the subsurface sediment. One may claim the denitrification as an evidence of the biogeochemical purification function of tidal flat. However, the purification seems not a general attribute of a tidal flat when whole system is scrutinized by a thermodynamic criterion. Currently the term 'tidal flat' is used when describing the diverse coastal wetlands such as salt marshes, sandy tidal flats and muddy tidal flats, which exhibit quite different ecological functions. Thus it is worthy of mentioning that the classification of coastal wetlands on the basis of sedimentological characteristics and biogeochemical functions should facilitate our understanding.

Heavy Metals in Surface Sediments from Doam Bay, Southwestern Coast of Korea (한국 남서해안 도암만 표층퇴적물의 중금속 함량 및 분포 특성)

  • CHO, HYEONG-CHAN;CHO, YEONG-GIL
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.20 no.4
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    • pp.159-168
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    • 2015
  • Forty-four surface sediments from Doam Bay were analyzed for total organic carbon (TOC), total nitrogen (TN), total metal (Al, Fe, Mn, Cr, Cu, Ni, Pb, Zn) and further chemical partitioning of metals were carried out in some samples. The TOC (0.32~3.10%) and TN (0.03~0.26%) values of the samples were similar to those of other coastal area. The C/N ratios ranged from 7.9 to 11.9 with an average 9.3 which revealed that contribution of terrestrial organic matters was relatively rare. Contents of analysed metals showed a level lower than threshold effects level (TEL) in sediment quality guidelines. Based on the chemical speciation of metals, the lattice fractions were found in the order Cr > Cu > Ni > Zn > Pb > Mn, while Mn and Pb are the ratio of the non-lattice fractions accounted for more than 50%. The average baseline values were obtained relative cumulative frequency curves and linear regression analysis. The respective baseline concentrations for Cu, Ni, Pb, Zn, Cr and Mn were 11.8, 23.1, 26.8, 76.6, 56.7, 585 mg/kg, respectively. Based on geoaccumulation index ($I_{geo}$) with a baseline values of Mn showed that face the contamination phase from estuarine stations. However, in case of Zn and Pb, although there is no sign of contamination, it could be release from sediment when there is a change in the environment, which is caused from the high ratio of non-lattice fractions.

Debris Flow Mobility: A Comparison of Weathered Soils and Clay-rich Soils (풍화토와 점성토 위주의 토석류 거동과 유동특성)

  • Jeong, Sueng-Won
    • Journal of the Korean Geotechnical Society
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    • v.29 no.1
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    • pp.23-27
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    • 2013
  • The risks of debris flows caused by climate change have increased significantly around the world. Recently, landslide disaster prevention technology is more focused on the failure and post-failure dynamics to mitigate the hazards in flow-prone area. In particular, we should define the soil strength and flow characteristics to estimate the debris flow mobility in the mountainous regions in Korea. To do so, we selected known ancient landslides area: Inje, Pohang and Sangju debris flows. Firstly we measured physical and mechanical properties: liquidity index and undrained shear strength by fall cone penetrometer. From the test results, we found that there is a possible relationship between liquidity index and undrained shear strength, $C_{ur}=(1.2/I_L)^{3.3}$, in the selected areas, even though they were different in geological compositions. Assuming that the yield stress is equal to the undrained shear strength at the initiation of sliding, we examined the flow characteristics of weathered soils in Korea. When liquidity index is given as 1, 1.5 and 3.0, the debris flow motion of weathered soils is compared with that of mud-rich sediments, which are known as low-activity clays. At $I_L=1$, it seems that debris flow could reach approximately 250m after 5 minutes. As liquidity index increased from 1 to 3, the debris flow propagation of weathered soils is twice than that of low-activity clays. It may be due to the fact that soil masses mixed with the ambient water and then highly fragmented during flow, thereby leading to the high mobility. The results may help to predict the debris flow propagation and to develop disaster prevention technology at similar geological settings, especially for the weathered soils, in Korea.

Antifungal Activity of Bacillus sp. GJ-1 Against Phytophthora capsici (Bacillus sp. GJ-1의 Phytophthora capsici에 대한 항진균활성)

  • Lee, Gun-Joo;Han, Joon-Hee;Shin, Jong-Hwan;Kim, Heung Tae;Kim, Kyoung Su
    • The Korean Journal of Mycology
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    • v.41 no.2
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    • pp.112-117
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    • 2013
  • Phytophthora capsici is one of major limiting factors in production of pepper and other important crops worldwide by causing foliage blight and rot on fruit and root. Increased demand for the replacement of fungicides has led to searching a promising strategy to control the fungal diseases. To meet eco-friendly agriculture practice, we isolated microorganisms and assessed their beneficial effects on plant health and disease control efficacy. A total of 360 bacterial strains were isolated from rhizosphere soil of healthy pepper plants, and categorized to 5 representative isolates based on colony morphology. Among the 5 bacterial strains (GJ-1, GJ-4, GJ-5, GJ-11, GJ-12), three bacterial strains (GJ-1, GJ-11, GJ-12) presented antifungal activity against P. capsici in an fungal inhibition assay. In phosphate solubilization and siderophore production, the strain GJ-1 was more effective than others. The strain GJ-1 was identified as Bacillus sp. using 16S rDNA analysis. Bacillus sp. GJ-1 was also found to be effective in inhibiting other plant pathogenic fungi, including Rhizoctonia solani, Pythium ultimum and Fusarium solani. Therefore, the Bacillus sp. GJ-1 can serve as a biological control agent against fungal plant pathogens.

Geochemical Characteristics of Surface Sediments in the Eastern Part of the Yellow Sea and the Korean West Coast (황해 동부 대륙붕과 한반도 서해안 표층퇴적물의 지구화학적 특성)

  • 조영길;이창복;박용안;김대철;강효진
    • The Korean Journal of Quaternary Research
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    • v.7 no.1
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    • pp.69-91
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    • 1993
  • A total of 76 surface sediment samples, collected from the Korean west coast and the eastern Yellow Sea areas, were analyzed for their elemental composition in order to understand the geochemical characteristics of these deposits. The analyzed elements included 9 major elements (Al, Fe, Na, K, Mg, Ca, Ti, P, Mn), 8 minor elements (Sr, Ba, V, Cr, Co, Ni, Cu, Zn), organic carbon and calcium carbonate. Contents of most analyzed elements, excluding K and Ba, were generally low compared to those of average crust. Contents of most elements, except K and Ca, also correlated with sediment grain size, though the degree of relationship varied widely from one element to another. For fine-grained sediments, a distinction could be made between those in the central Yellow Sea and those in the Keum Estuary based on their characteristic elemental composition: the former were rich in Fe, Na, K, Mg, Ca and V, and the latter in Mn, Co and Ni. The element/aluminium ratios, on the other hand, showed that the central Yellow Sea muds were enriched in Fe, Mg, V, Ni, Cu and Zn and depleted in K, Mn, Ba and Sr relative to the mud located near the Korean Peninsula. Based on the analysis of these results, as well as of the influences of particular mineral phases or pollution effects, we could suggest geochemical criteria which can be used in distinguishing muds from the two different sources, the Keum River and the Yellow River: the former by the higher Mn content and the latter by the higher Mg and V contents, relative to each other.

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Surficial Sediment Classification using Backscattered Amplitude Imagery of Multibeam Echo Sounder(300 kHz) (다중빔 음향 탐사시스템(300 kHz)의 후방산란 자료를 이용한 해저면 퇴적상 분류에 관한 연구)

  • Park, Yo-Sup;Lee, Sin-Je;Seo, Won-Jin;Gong, Gee-Soo;Han, Hyuk-Soo;Park, Soo-Chul
    • Economic and Environmental Geology
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    • v.41 no.6
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    • pp.747-761
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    • 2008
  • In order to experiment the acoustic remote classification of seabed sediment, we achieved ground-truth data(i.e. video and grab samples, etc.) and developed post-processing for automatic classification procedure on the basis of 300 kHz MultiBeam Echo Sounder(MBES) backscattering data, which was acquired using KONGBERG Simrad EM3000 at Sock-Cho Port, East Sea of South Korea. Sonar signal and its classification performance were identified with geo-referenced video imagery with the aid of GIS (Geographic Information System). The depth range of research site was from 5 m to 22.7 m, and the backscattering amplitude showed from -36dB to -15dB. The mean grain sizes of sediment from equi-distanced sampling site(50 m interval) varied from 2.86$(\phi)$ to 0.88(\phi). To acquire the main feature for the seabed classification from backscattering amplitude of MBES, we evaluated the correlation factors between the backscattering amplitude and properties of sediment samples. The performance of seabed remote classification proposed was evaluated with comparing the correlation of human expert segmentation to automatic algorithm results. The cross-model perception error ratio on automatic classification algorithm shows 8.95% at rocky bottoms, and 2.06% at the area representing low mean grain size.

Investigation of Dynamic Elemental Distribution in Six Human Tissues by Neutron Activation Analysis (중성자방사화분석에 의한 인체 6개 조직 내 기능성원소 함량 분포 조사)

  • Kang, Sang-Hoon;Moon, Jong-Hwa;Chung, Yong-Sam;Cho, Seung-Yeon;Lee, Han-Young
    • Analytical Science and Technology
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    • v.15 no.4
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    • pp.365-372
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    • 2002
  • In this study, inorganic elemental distribution in Korean adult human tissue of liver, lung, muscle, kidney, rib and thyroid has been investigated. These tissues were collected from human's body within 48 hours after their death according to IAEA's recommended method, dried by freeze drying method and then pulverized. Neutron activation analysis was applied to these samples to determine the concentrations of 5 major elements of Ca, Mg, Na, K, and Cl and 12 trace elements of Mn, I, Sr, As, Br, Co, Cr, Cs, Fe, Rb, Se, and Zn. To confirm accuracy of analytical result was performed using three kinds of standard reference materials and resulted that the relative error is within 10% for the most of elements and the Z-score indicating a performance of analysis is within 2. The elemental distribution shows that the major elements are distributed homogeneously with a specific concentration and that the trace elements shows a distribution of different concentration with kinds of tissue and their age. Particularly, iodine shows higher concentration with 1000 ${\mu}g/g$ in thyroid, and calcium and strontium show the concentration with 15.9% and 84.4 ${\mu}g/g$ in rib, respectively.