• Korean Journal of Head & Neck Oncology
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• v.12 no.2
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• pp.169-176
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• 1996

Tuberculous cervical lymphadenitis is still an important cause of neck mass in Korea. Tuberculosis is an important differential diagnosis in patients of cervical lymphadenopathy. Rapid and sensitive test for the diagnosis of tuberculosis is essential for the approapiate treatment. Up to now, conventional diagnostic methods for M. tuberculosis were acid-fast bacilli(AFB) stain and culture of M. tuberculosis. The direct microscopic examination of AFB by Ziehl-Neelsen stain is rapid, but often negative. The culture for M. tuberculosis is time-consuming, taking 4 to 8 weeks. Recently various methods to detect Mycobacterial DNA, including PCR and restriction fragment length polymorphism(RFLP) analysis have been reported. Here we represent a simple method for the confirmation of M. tuberculosis and exclusion of the other Mycobacterial species by RFLP analysis and silver staining of polyacrylamide gel electrophoresis after nested PCR for a repetitive DNA sequence(IS986) specific for M. tuberculosis from fresh or paraffin-embedded biopsy specimens. This result leads us to conclude that this method is simple, rapid and possibly applicable to confirm M. tuberculosis and rule out the other Mycobacteria species from the clinical specimens in the clinical laboratories.

• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.103-108
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• 2020

Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacteria and can cause severe foodborne disease. For STEC detection, conventional culture methods have disadvantages in the fact that conventional culture takes a long time to detect and PCR can also detect dead bacteria. To overcome these problems, we suggest a bacteriophage amplification assay, which utilizes the ability of bacteriophages to infect living cells and their high specificity. We used a combination of six bacteriophages infecting E. coli to make the bacteriophage cocktail and added ferrous ammonium sulfate as a virucidal agent to remove free-bacteriophages. When cherry tomato and paprika were artificially inoculated with the cocktail at a final concentration of around 3 log CFU/mL and were enriched for at least 5 h in mTSB broth with Novobiocin, approximately 2-3 log PFU/mL were detected through the bacteriophage amplification assay. Therefore, bacteriophage amplification assay might be convenient and a useful method to detect STEC in a short period of time.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.847-858
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• 2015

Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor - of which soil was put in 14 mL tube after treatment - and field environments. Soil samples were collected over a four-week period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g $soil^{-1}$ immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g $soil^{-1}$) was similar to that of the control (6.38 log copies g $soil^{-1}$). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.

• Journal of Food Hygiene and Safety
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• v.26 no.2
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• pp.120-124
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• 2011

Predictive mathematical models were developed for predicting the kinetics of growth of Listeria monocytogenes in smoked salmon, which is the popular ready-to-eat foods in the world, as a function of temperature (4, 10, 20 and $30^{\circ}C$). At these storage temperature, the primary growth curve fit well ($r^2$=0.989~0.996) to a Gompertz equation to obtain specific growth rate (SGR) and lag time (LT). The Polynomial model for natural logarithm transformation of the SGR and LT as a function of temperature was obtained by nonlinear regression (Prism, version 4.0, GraphPad Software). Results indicate L. monocytogenes growth was affected by temperature mainly, and SGR model equation is $365.3-31.94^*Temperature+0.6661^*Temperature^{\wedge^2}$ and LT model equation is $0.1162-0.01674^*Temperature+0.0009303^*Temperature{\wedge^2}$. As storage temperature decreased $30^{\circ}C$ to $4^{\circ}C$, SGR decreased and LT increased respectively. Polynomial model was identified as appropriate secondary model for SGR and LT on the basis of most statistical indices such as bias factor (1.01 by SGR, 1.55 by LT) and accuracy factor (1.03 by SGR, 1.58 by LT).

• Journal of the Economic Geographical Society of Korea
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• v.4 no.2
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• pp.57-77
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• 2001

The Honam region has been recognized as one of the most lagging regions in Korea. Drawing upon some decompositions oi Gross Regional Domestic Product(GRDP) per head and labor productivity, the overall trend of inequalities in Korea has been revealed and the trajectory of the Honam region's economic change has been identified by examining how the region's economic performance changed relative to that of Korea in the 1990s. There are some findings. First of all, Korea is characterized by strong divergence in the development of its region economies, in terms o\ulcorner per capita GRDP, which relies upon productivity differentials rather than differences in employment rates. Second, the Honam region has displayed poor economic performances in terms of GRDP per head except for Jeonnam. Third, the dramatic fall in cumulative population growth relative to the national average has been an important factor in the Jeonnam's overwhelming economic performance measured by per capita GRDP. Fourth, the decline in the relative productivity of the Honam region is mostly explained by falling relative regional productivity growth in construction, transport and manufacturing, although it should be noted that the latter sector has made positive contribution to raising relative regional productivity of Jeonnam. Fifth, overall the shift in employment to service sector, especially to social, personal, public, health, education, other services other than distribution, hotels, catering has tended to support relative regional productivities, along with the positive contribution being made oi agriculture, forestry and fishing to relative regional productivity growth in the Honam region.

• Journal of Korea Water Resources Association
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• v.44 no.12
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• pp.929-940
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• 2011

In this study, the future expected discharges are analyzed for Daecheong and Yongdam Dam Watershed in Geum River watershed using A1B scenario based RCM with 27 km spatial resolutions from Korea Meteorological Agency and SWAT model. The direct use of GCM and RCM data for water resources impact assessment is practically hard because the spatial and temporal scales are different. In this study, the problems of spatial and temporal scales were settled by the spatial and temporal downscaling from watershed scale to weather station scale and from monthly to daily of RCM grid data. To generate the detailed hydrologic scenarios of the watershed scale, the multi-site non-stationary downscaling method was used to examine the fluctuations of rainfall events according to the future climate change with considerations of non-stationary. The similarity between simulation and observation results of inflows and discharges at the Yongdam Dam and Daecheong Dam was respectively 90.1% and 84.3% which shows a good agreement with observed data using SWAT model from 2001 to 2006. The analysis period of climate change was selected for 80 years from 2011 to 2090 and the discharges are increased 6% in periods of 2011~2030. The seasonal patterns of discharges will be different from the present precipitation patterns because the simulated discharge of summer was decreased and the discharge of fall was increased.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.71-71
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• 2011

지난 세기동안 지구 평균 기온이 상승함에 따라 대기 중에 차지하는 수증기 함유량 또한 증가 추이를(7%/$^{\circ}C$) 보이고 있으며, 이는 전 세계적으로 수문 순환 패턴의 변화를 초래한다(IPCC, 2007). 그 중에서도 강수 특성의 변화는 궁극적으로 유출량의 변화를 초래하며, 이는 수자원 총량의 변화로 이어지게 된다. 특히, 여름철에 대부분의 강수 현상이 집중되는 우리나라의 경우 육지의 70% 정도가 산악 지형으로 이루어진 복잡한 지리적 영향으로 집중호우 시 홍수가 일시에 유출되어 이에 따른 인적 물적 피해가 해마다 되풀이 되고 있다. 수자원은 인간 생활과 밀접한 관계에 있기 때문에 이러한 극심한 기후변화에 의한 피해를 최소화하기 위해 수계단위의 효율적인 물관리가 필수적이다. 따라서 한반도 내 주요 강(한강, 금강, 영산강, 섬진강, 낙동강)을 중심으로 수계별 강수량 및 유출량의 장기 특성 변화를 살펴보고자 한다. 장기간의 자료를 보유하고 있는 기상청 산하 27개 지점의 시간 강수량 자료 및 국가 수자원관리 종합정보시스템에서 제공하는 장기유출 자료를 수집하여 수계 평균값을 산정하고, 각 수계별 강수량 및 유출량의 장기 추이 및 변동성, 상관도를 알아보고자 하였다. 최근 36년 동안(1973~2008년) 모든 수계에서 연총강수량이 증가하는 추이를 보였으며, 한강 수계에서 유의수준 5% 내에서 가장 높은 증가율(약 10 mm/yr)을, 섬진강 수계에서 가장 낮은 증가율(약 4 mm/yr)을 나타냈다. 여름철 집중호우(20 mm/hr 이상) 빈도 분석 결과, 모든 수계에서 호우 빈도의 증가 경향이 뚜렷함을 볼 수 있다. 특히, 최근 10년간(1999~2008) 호우빈도의 변화를 살펴보면 섬진강 수계의 경우 총 60번으로 가장 많았고 상대적으로 낙동강 수계에서 35번으로 가장 적었다. 여름철 무강수일수(강수량이 0.1 mm 미만인 일수)의 경우 모든 수계에서 거의 완만한 감소추세를 보임을 확인할 수 있었다. 1970~2001년간 연총유출량의 경우 한강 및 금강 수계의 경우 증가하는 경향을 나타내는 반면 섬진강 수계의 경우 오히려 감소하며, 영산강 및 낙동강 수계에서는 뚜렷한 변화를 볼 수 없었다. 월별 유출량의 경우 모든 수계에서 7월, 8월, 9월에 집중되며, 한강 수계에서 8월, 그 외 수계에서는 7월에 가장 높은 값을 보였다. 향후 장기적인 관점에서 바라 본 강수량과 유출량의 관계에 관한 추가적인 연구를 통하여 신뢰성 있는 기후변화에 따른 수자원 영향 평가에 기여할 수 있을 것으로 사료된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.135-135
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• 2011

지구온난화와 이상기후에 따라 최근 우리나라를 둘러싼 기후패턴의 변화가 가속화되고 있으며 한반도는 장마기간이 소강상태를 보이는 반면, 장마 후 국지성 집중호우가 증가하고 태풍이 내습하는 현상이 빈번해짐으로써 홍수에 대한 위험과 피해규모도 증가하고 있다. 특히 도시지역에서는 강우규모가 배수시스템의 용량을 초과하거나, 하천수위 상승으로 관로 내에 역류가 발생하는 등 우수 배제 기능을 제대로 수행하지 못할 경우 발생하는 지표침수로 인해 심각한 인명 및 재산피해가 발생하고 있다. 실제로 현재 홍수해석 및 홍수위험지도 작성시 내수시스템을 반영하지 않아 침수면적 및 범위의 오차가 존재하며 홍수위험지도 작성시 내수범람과 외수범람을 따로 고려하는 문제점이 있다. 따라서 도시 침수 해석시 내수시스템을 반영한 정확한 침수심 및 침수면적계산뿐만 아니라 이상기후에 대비한 복합적 요인으로 인한 침수해석이 필요하다. 따라서 본 연구에서는 하천해석을 위해 1차원 하천 해석 모형인 FLDWAV모형을 적용하고 가상의 제방 파제 시나리오를 통하여 외수범람 영향을 구하였으며, 배수시스템의 SWMM모형과 제내지에서 내수와 외수범람의 영향을 고려한 DEM기반의 2차원 범람해석을 연계한 Dual-Drainage모형에 대하여 외수범람 영향에 따른 흐름의 양상, 침수심, 침수위 등을 분석하였다. 개발한 모형에 대한 적용성을 검토하기 위하여 대구 신암5동 유역을 선정하였고 대상유역의 수치지도를 활용하여 정형 격자 20m 크기로 지형자료를 구성하였으며, 건물의 영향도 고려하기 위해 DEM에 건물자료를 합성하였다. 침수해석 결과 내수시스템의 영향을 고려하지 않을 때가 고려하였을 때 보다 Node(맨홀)에서의 재유입의 영향으로 인하여 최대 침수심이 더 높게 나왔으며 침수면적도 넓게 나타나는 것을 확인하였고, 기존의 홍수위험지도 작성시 외수침수와 내수침수를 구분하여 해석하였던 것을 본 연구에서 통합하여 외수범람의 영향을 고려한 통합침수해석을 실시하여 내수에서 발생할 수 있는 유출량과 내수시스템의 월류량 등에 대한 고려가 없는 외수침수만 해석시 보다 최대침수심이 더 높게 나타났으며 침수면적 또한 넓게 나타남을 알 수 있었다. 본 연구를 통해서 도시홍수, 돌발홍수 등의 발생시 정확한 도시 침수 해석이 가능하며 도시침수구역에 대한 적절한 예 경보 및 피난대책 수립에 활용될 수 있을 것으로 사료된다. 또한 국내의 홍수위험지도나 도시 침수해석과 연계하여 선행시간을 확보한 정확도 높은 홍수정보시스템 구축에 크게 기여할 것으로 판단된다.

• Journal of KIISE
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• v.42 no.4
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• pp.451-458
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• 2015

Previous multimodal learning methods focus on problem-solving aspects, such as image and video search and tagging, rather than on knowledge acquisition via content modeling. In this paper, we propose the Multimodal Concept Hierarchy (MuCH), which is a content modeling method that uses a cartoon video dataset and a character-based subtitle generation method from the learned model. The MuCH model has a multimodal hypernetwork layer, in which the patterns of the words and image patches are represented, and a concept layer, in which each concept variable is represented by a probability distribution of the words and the image patches. The model can learn the characteristics of the characters as concepts from the video subtitles and scene images by using a Bayesian learning method and can also generate character-based subtitles from the learned model if text queries are provided. As an experiment, the MuCH model learned concepts from 'Pororo' cartoon videos with a total of 268 minutes in length and generated character-based subtitles. Finally, we compare the results with those of other multimodal learning models. The Experimental results indicate that given the same text query, our model generates more accurate and more character-specific subtitles than other models.

• Journal of KIISE:Computer Systems and Theory
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• v.36 no.5
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• pp.412-421
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• 2009

Due to the physical characteristics of NAND flash memory, overwrite operations are not permitted at the same location, and therefore erase operations are required prior to rewriting. These extra operations cause performance degradation of NAND flash memory file system. Since it also has an upper limit to the number of erase operations for a specific location, frequent erases should reduce the lifetime of NAND flash memory. These problems can be resolved by delaying write operations in order to improve I/O performance: however, it will lower the cache hit ratio. This paper proposes a policy of page management using double cache for NAND flash memory file system. Double cache consists of Real cache and Ghost cache to analyze page reference patterns. This policy attempts to delay write operations in Ghost cache to maintain the hit ratio in Real cache. It can also improve write performance by reducing the search time for dirty pages, since Ghost cache consists of Dirty and Clean list. We find that the hit ratio and I/O performance of our policy are improved by 20.57% and 20.59% in average, respectively, when comparing them with the existing policies. The number of write operations is also reduced by 30.75% in average, compared with of the existing policies.