• Korean Journal of Microbiology
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• v.30 no.4
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• pp.291-298
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• 1992

Srrepromycec. corlirolar produces at least 4 catalase activity bands with different electrophoretic mobilities on polyacrylamide gel which vary during development. Spores and mycelia at stationary phase produced all the activity bands(Cat1. 760 kr); Cat3-I, 170 kD: Cat3-2, 140 kD: Cat3-3. 130 kD; Cat4, 70 kD) except for Cat2 (300 kD). Mycelia at mid-logarithmic phase produced only Cat2 and Cat3-2 bands, and mycelia at late-logarithmic phase produced bands except Catl and Cat\ulcorner. Catalase-deficient mutants were screened in S. coelicalur by H201 bubbling test following NTG mutagenesis. Wc tested sevcral non-bubbling or slow-bubbling mutants for their catalase activities. The overall activities in cell extracts decreased more than 5 fold. Activity bands in native gel selectively decreased in intensity or disappeared. In all the non-bubbling mutants testcd, Cat3-2 band decreased significantly or disappeared. suggesting that Cat3-2 is the major catalase. The selective disappearance of bands in mutants suggest that each band is governed by different genes. We purified catalase activity from -:ell extracts obtained at late-logarithmic phase. Following chromatographies on Sepharose CL-4B. DEAE Sepharose CL-6B. Phcnyl Sepharose CL-4B. and hydroxylapatite columns. only the Cat3-2 activity was obtained. The native form of Cat3-2 has molecular weight of approximately 140 kD, judged by gel electrophoresis. Thc electrophoretic mobility on SDS-polyactylamide gel suggests that this enzyme contains 2 identical subunits of 67 kD.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.362-369
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• 1996

Seven different drying methods were tested in peony roots of Euisung cultivar, harvested in February, in three year's old plant. The roots were selected in length and diameter and half of the samples were removed cork-layers to compare the effects of cork-layer in processing of drying. The 3$0^{\circ}C$ heat drying without cork-layer reduced in days to drying by five days compared to those of the with cork-layers at the same temperature. The 5$0^{\circ}C$ heat drying after cork-layers removed was the most effective in days to drying. In quality of skin color of the 5$0^{\circ}C$ heating was worse to compare with the lower drying temperature. In the drying at room temperature and the heat drying at lower temperature, the paeoniflorin content in drying after cork-layers removed were higher than that of the drying with cork-layers. However, in the boiling water treatment, the paeoniflorin contents in drying after cork-layers removed were lower than those of with cork-layers. In heat drying, paeoniflorin content showed a decreasing tendency to increase of drying temperature. Total sugars in the peony roots showed a decreasing tendency according to the drying temperature increasing, but starch concentration showed a increasing tendency at the same condition. Concentrations of crude protein, crude fiber and crude ash were showed no differences in various drying methods and the materials with or without cork-layers. Relationships between the paeoniflorin and total sugars, and the paeoniflorin and starch were different significantly by the materials of cork-layers removed or not. The 30~4$0^{\circ}C$ heat drying without cork-layers was the most advisable condition for drying in paeoniflorin concentration, days to drying and skin color after drying.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.269-274
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• 2007

This experiment was conducted to determine the effect of seeding method on winter survival and dry matter yield of Italian ryegrass(Lolium multiflorum Lam. "Kospeed") in paddy field for two years. The experiment was arranged in randomized complete block design with three replications. The treatment consisted of four different seeding methods, R-LS-P(rotary-line sowing-packing), R-BS-P(rotary-broadcast seeding-packing), R-BS(rotary-broadcast seeding), R-BS-TR(rotary-broadcast seeding-trifling rotary), BS-TR(broadcast seeding-trifling rotary). The emergence ratio of broadcast was decreased by 12% and winter survival was decreased by 17% compared with line sowing. The R-BS-TR treatment which was practical seeding method of rye was decreased seeding stand ratio by 65.2% and winter survival by 6.7% compared with others seeding methods. Dry matter yield of line sowing(8,151 kg/ha) was higher than that of broadcast(6,281 kg/ha) and R-BS-P treatment was the highest DM yield as 7,166 kg/ha. There was not found significant difference among seeding method in forage quality. The results of this experiment indicated that R-BS-P(rotary-broadcast seeding-packing) was recommendable the best seeding method after harvested whole crop rice in paddy field.

• Korean Journal of Food Science and Technology
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• v.45 no.3
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• pp.305-311
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• 2013

Effects of cultivation methods on quality characteristics, pasting characteristics, chemical components, and antioxidant activities of sweet potatoes (Ipomoea batatas (L.) Lam) were determined. The Brix degree, hunter color value, pasting characteristics, moisture, protein, and mineral contents of the sweet potatoes showed significant differences from cultivation methods. The total polyphenol and flavonoid contents of the methanolic extracts of the sweet potato's pericarp were higher than sweet potato's sarcocarp. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the sweet potato's pericarp on the conventional culture and successful cropped hairy vetch culture was 776.38 and 715.20 mg TE/100 g sample. The 2,2'-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) radical scavenging activity of the sweet potato's pericarp on the conventional culture and successful cropped hairy vetch culture was 708.03 and 708.58 mg TE/100 g sample. Generally, there was a difference in antioxidant compound content and radical scavenging activity on the methanolic extract of sweet potato with cultivation methods.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.845-852
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• 2014

Among various abiotic stress factors, soil salinity decreases the photosynthetic rate, growth, and yield of plants. Recently, many genes have been reported to enhance salt tolerance. The objective of this study was to characterize the Brassica rapa Salt Stress Resistance (BrSSR) gene, of which the function was unclear, although the full-length sequence was known. To characterize the role of BrSSR, a B. rapa Chinese cabbage inbred line ('CT001') was transformed with pSL94 vector containing the full length BrSSR cDNA. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of BrSSR in the transgenic line was 2.59-fold higher than that in the wild type. Analysis of phenotypic characteristics showed that plants overexpressing BrSSR were resistant to salinity stress and showed normal growth. Microarray analysis of BrSSR over-expressing plants confirmed that BrSSR was strongly associated with ERD15 (AT2G41430), a gene encoding a protein containing a PAM2 motif (AT4G14270), and GABA-T (AT3G22200), all of which have been associated with salt tolerance, in the co-expression network of genes related to salt stress. The results of this study indicate that BrSSR plays an important role in plant growth and tolerance to salinity.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.191-196
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• 1998

This experiment was conducted to investigate the mulch effect of rice straw and dead water foxtail treated with herbicides on weed germination, seedling establishment, and early growth of rice under the no-till culture condition. The percent seedling establishment of rice straw + dead water foxtail mulch was decreased significantly compared to rice straw mulch and non-mulch. Rice straw mulch and rice straw + dead water foxtail mulch inhibited the early growth of no-till direct seeded rice remarkably. However, they promoted rice growth after active tillering stage. Rice straw mulch and rice straw+dead water foxtail mulch in no-till direct seeding decreased weed occurrence significantly. The control value of barnyardgrass, compared with non-mulch, was 96.5% in the rice straw mulch and 75.6% in the rice straw + dead water foxtail mulch, respectively. The pH and concentration of dissolved oxygen in irrigated water were reduced significantly by both rice straw mulch and rice straw+dead water foxtail mulch during early rice growth stage.

• Journal of Mushroom
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• v.12 no.1
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• pp.29-34
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• 2014

Mycelial viability and cultivation characteristics of strains of Hypsizygus marmoreus after long-term storage were investigated. The experimental conditions were the storage at $4^{\circ}C$ using a slant culture technique with or without mineral oil, and in liquid nitrogen tank in the presence of 10% glycerol or 10% glycerol with 5% trehalose. The myceila of four strains of H. marmoreus were thawed at 9, 21, 33, and 45 months after beginning of the storage, and then the growth of the mycelia was measured on a PDA plate with serial transfers to new plate for the recovery. The mycelial growth data after 45 months showed that the mycelia were mostly viable but not fully active particularly when they were stored in liquid nitrogen with 10% glycerol. The growth activity could be fully recovered after second transfer to new PDA plate. Cultivation of mushroom fruiting body using the recovered mycelia also demonstrated that the storage methods employed in this work were applicable for the long-term storage of H. marmoreus.

• Food Science and Preservation
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• v.11 no.2
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• pp.250-256
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• 2004

The objectives of this study were to develop a new commercial grain cooler suited to domestic weather and post-harvesting conditions for paddy, and to evaluate the performance. A prototype grain cooler capable of cooling paddy of 200 tons within 24 hours was developed. The grain cooler was designed to control the refrigeration capacity from 0 to 100% by controlling the capacity of compressor with unloading solenoid valve and by changing the flow rates of hot refrigerant gas flowing into reheater and evaporator from compressor. And a controller with one chip microprocessor was developed to control temperature and relative humidity of cooling air. The maximum cooling capacity of the grain cooler was 35,284㎉/hr at condensing/evaporating pressure of 16.5/3.6 kgf/$\textrm{cm}^2$. Maximum flow rate of cooling air was 120 ㎥/min at static pressure of 279 mmAq. The total maximum required power was 22.8㎾, and total required energy was saved from 26.7 to 33.3% of maximum power depending on operating conditions. The coefficient of performance of refrigeration devices and total coefficient of performance of the grain cooler were 4.71 and 1.8, respectively.

• Food Science and Preservation
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• v.20 no.3
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• pp.296-303
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• 2013

This study was conducted to assess the possibility of preserving frozen vegetables (Aster scaber, soybean sprouts, Chinese cabbage, green pumpkin, and Welsh onion) for a long period and of using them after such storage by measuring changes in quality due to their preservation. Various freshly harvested vegetables were blanched under optimal conditions (that were determined in a preliminary experiment), quick-frozen at $-40^{\circ}C$ for 24 h, and stored at $-20^{\circ}C$. The change in the chromaticity of the frozen A. scaber, soybean sprouts, Chinese cabbage, green pumpkin, and Welsh onion did not vary. The hardness of the frozen A. scaber, green pumpkin, Chinese cabbage and Welsh onion did not change during the preservation period, whereas the hardness of the cotyledon and hypocotyl of the soybean sprouts significantly increased on the sixth month of their storage. The total bacterial counts of the A. scaber during the preservation period remained constant at $10^3$ CFU/g, whereas those of soybean sprouts, Chinese cabbage, green pumpkin, and Welsh onion decreased slightly to $10^2$ CFU/g. Coliform was not detected in any of the samples. The sensory evaluation showed that the preservation period that was used in this study did not significantly affect the marketability of the frozen vegetables. Therefore, it was considered that A. scaber, soybean sprouts, Chinese cabbage, green pumpkin, and Welsh onion can be safely preserved by freeze-storing them for up to 12 months.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.221-227
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• 2005

Apoptosis of Prunus salicina Lindl. cv. Soldam, which possesses hematopoiesis, osteoporosis prevention, and antimutagenic effects, at different growth stages was evaluated. Cytotoxic effect of acetone extracts of immature fruits against various tumor cell lines was higher than that of mature fruits, particularly in hormone-independent human breast cancer, MDA-MB-231 cell line. Immature fruit extract increased expression level of pro-apoptotic protein Bax and reduced that of anti-apoptotic protein Bcl-2, and stimulated caspase-3 activity in MDA-MB-231 cells. Results suggest immature fruit of P. salicina Lindl. cv. soldam to be natural source for development of functional food and medical agents to prevent human breast cancer.