Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Induction of Apoptosis by Immature Prunus salicina Lindl. cv. Soldam

피자두(Prunus salicina Lindl. cv. Soldam) 미숙과의 apoptosis 유도 효과

  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University) ;
  • Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University) ;
  • HwangBo, Mi-Hyang (Department of Food Science and Technology, Keimyung University) ;
  • Lee, Ji-Won (Department of Food Science and Technology, Keimyung University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 유미희 (계명대학교 식품가공학 전공) ;
  • 임효권 (계명대학교 식품가공학 전공) ;
  • 황보미향 (계명대학교 식품가공학 전공) ;
  • 이지원 (계명대학교 식품가공학 전공) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터)
  • Published : 2005.04.30
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Abstract

Apoptosis of Prunus salicina Lindl. cv. Soldam, which possesses hematopoiesis, osteoporosis prevention, and antimutagenic effects, at different growth stages was evaluated. Cytotoxic effect of acetone extracts of immature fruits against various tumor cell lines was higher than that of mature fruits, particularly in hormone-independent human breast cancer, MDA-MB-231 cell line. Immature fruit extract increased expression level of pro-apoptotic protein Bax and reduced that of anti-apoptotic protein Bcl-2, and stimulated caspase-3 activity in MDA-MB-231 cells. Results suggest immature fruit of P. salicina Lindl. cv. soldam to be natural source for development of functional food and medical agents to prevent human breast cancer.

본 연구에서는 생육시기별로 피자두를 수확한 후 피자두의 아세톤 추출물을 이용하여 인간 유래 간암, 위암, 자궁암, 백혈암 및 유방암 세포주에 대한 세포독성효과를 조사하였고, 특히 유방암 세포주인 MDA-MB-231 세포주를 사용하여 피자두의 유방암 세포의 세포주기에 미치는 영향과 세포사 관련 조절 단백질의 발현 양상을 분석함으로써 피자두의 유방암 억제 기전을 살펴보았다. 인간유래의 암세포주를 이용하여 피자두 추출물의 성장 저해 효과를 측정한 결과, 농도 의존적으로 모든 암세포주에 대해 성장 저해 효과를 보였으며, 특히 피자두 1-4의 미숙과 추출물에서 높은 성장 저해 효과를 보였다. 특히 호르몬 비의존성 유방암세포인 MDA-MB-231에 대한 피자두 추출물의 성장 저해 효과는 4mg/mL의 농도의 모든 추출물에서 높은 효과를 보였으며, 피자두 1의 추출물에서는 97%의 높은 성장 저해 효과를 보였다. MDA-MB-231 세포주에 피자두 미숙과 추출물을 처리했을 때 apoptosis를 유도하는 유전자인 Bcl-2 family에 영향을 미치는 것으로 보여지며, 순차적으로 capase-3 활성이 촉진되고, 이것에 의해 apoptosis가 유도되는 것으로 생각된다. 앞으로 이들 활성성분들의 규명과 인체실험과 더불어 피자두 미숙과에 대한 독성평가가 병행된다면 항암과 관련된 기능성 식품과 치료제 등의 개발에 있어 천연소재로서 피자두 미숙과의 유용성을 기대할 수 있을 것으로 생각된다.

Keywords

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